FastQCFastQC Report
Sat 31 Dec 2022
SRR5844522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62524
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT18612.976457040496449No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC16062.5686136523574947No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC3510.5613844283795023No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA2820.4510268057066087No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2440.39025014394472524No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA2110.337470411362037No Hit
TGACTGGGGGTGAGGACTGTTCCCATCCAGGCCCAGCTGGGGCTTTCCCTTT1880.3006845371377391No Hit
GATCATGGGAGGAATTCAACATCTAGAAGTAATTCATAATATTTTTGGATAT1630.2606998912417632No Hit
TTGCATGGGCAACATATCCAAAATATTATATCAACAGGTAATTAATATAAAA1600.2559017337342461No Hit
TCGTCAGGGGCTCTCTCCTCCCGCCGCCCAAGATGCCGAAAGGAAAGAAGGC1560.2495041903908899No Hit
CTGGGTGGGAGAATTCTTAAACTCTGTGATTTAGCTGTTTCAGTAAATAAAT1420.22711278868914336No Hit
GCTCCGGGGAGTTTCCATTTATGTCAATGCTAAATCCAAAGTCACTTCAGAG1360.21751647367410915No Hit
TATTTGTGGGGTGACCAATAGCATCTCATGCATCCTAGAGCTTCCAGTCTAG1330.212718316166592No Hit
ACAACCGGGCTGAAGACAGAGCAAAATGCTTTGAAAAGAATGAGGCCATACA1310.20951954449491395No Hit
GCATGCGGGAAGGTCTCATCTCACTCTCTTGCCCAGGCTAGAGTGCAGTGGC1270.2031220011515578No Hit
ACACACGGGATTCAACACAGTTTTCAACAGTGCTACTGAAAGCTACAGGAGA1250.19992322947987975No Hit
GCACTGGGGATATCCTGTGACTTAGAGTGTAGGATTTGTCATAAAAATCAGC1230.19672445780820166No Hit
TGGCTGGGATTGTTCTTCAAGCATGTTTTAGACCTAGAAAAGTATGGTTTGG1220.19512507197236262No Hit
GCGCGGGGGCATCCGGCGACAGAGGACAGCCAGAAGCCCAGCCAGCCCCAGA1210.19352568613652357No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1200.19192630030068455No Hit
TTGAAAGGGACAGCCAGCCCAGCTCCACTCAGGGCTGGCCCGGGGAGTCCCC1200.19192630030068455No Hit
AACGGGAAGGCCAGCTACAGGTGGACTCCTGGAATTTGAGGCATCATAATGA1140.1823299852856503No Hit
TGTCGCGGGATAGGATATTAGTACATCGTACACGTGCTAGGAAAAAACAGCT1140.1823299852856503No Hit
AAACGCGGGATGTACAGATGTACAAACATCATTTTAGGGTTTAATTTGTTTT1130.18073059944981126No Hit
GCTGTAGGGATGTCTTTGTTATCTTATTACAAGAATTGTTATTTGAAAAAGA1110.1775318277781332No Hit
CTACGATGGGATTTCCCAGATTAAAAATACCAAAACTATTTTTTAAACTTAA1100.17593244194229415No Hit
CTCACTGGGATGAGAACTTCAGGAAAAAGCAGATTGAAGAGCTGAAGGGACA1100.17593244194229415No Hit
AGTCATGGGATGTACATAATGGAATACTATTCAGCCATAAAAAAGAATGAAA1070.17113428443477705No Hit
GATGCTGGGAGTTCTCAGCTGTTTACCACAGCCCTCCAGGGTCCAAAGATTG1070.17113428443477705No Hit
ACGTTTGGGATTCCATTTTCCTAGTGCCACATGTGAAATTGGATTTTGATGA1060.16953489859893803No Hit
ACATGTGGGGATCAAATAGAATGGCTTTCTGGAATGACTAATTTGAAATATT1060.16953489859893803No Hit
AAGGCTGGGAATGTTGGGGAAAGTGAATTTTTCTCCTTTGTAGAAGGAGGTA1050.16793551276309898No Hit
AAACATGGGAGCTCTCAAAATCCACTGAAGAAGTCAAGTGAAGGTGGGTAAA1040.16633612692725994No Hit
TGGCCGGGGCAAGATGTGCTTCTCTGCAATAACCAAAGACCTGAGCATGTCA1010.16153796941974283No Hit
TAAGGTGGGAAGACATGGTTATTGACCTACTGCTTGACCAACTGACAGACCA1000.15993858358390378No Hit
CATAGCGGGGCCACCATGCCTGGCTAATTTTTTGTGTGTTTTTAGTACAGAC990.15833919774806474No Hit
AAATGTGGGAGTTGACAGCATATTAATTGTGTGGCATTATAATTGTGAGAAA980.1567398119122257No Hit
AAAGCTGGGGAGATCAAAAATGGCGGTAGTTGGTGTGTCCTCGGTTTCTCGG960.15354104024054763No Hit
GCTCGGGGACGTGCGCCACCACACCCAGTTCATTTTTGTATTTTTAGTAGAG960.15354104024054763No Hit
GTGGACGGGAACCGACTAATCACCACCCAACAATGACTAATCAAACTAACCT960.15354104024054763No Hit
CGCTATGGGATGTGCATCTCTGTCAGCCTGAGTGGCTTTGTGGTCTTGGGCT950.1519416544047086No Hit
GGCCAAGGGATCTGACTGAGAAAATAGTTATTTCAGAGATAAAATAAGGAAA940.15034226856886956No Hit
GTATGGGGATTACAAAAGAAGAAACTGAGGGTCTCTGAGGTTAAGTAGCTTT940.15034226856886956No Hit
AACTTAGGGAGGAGAGGAAAAGGTGTAGGCCTGCCTTACCGAGAGCCAAACA940.15034226856886956No Hit
TATCACGGGGTGTGGTAAAATTGGGGTAAAATGTGGTAAAATGTGATACGCA930.14874288273303052No Hit
CAAGCGGGGATGCCTTGAAAACCAGGAGAATATGGAATAAAACTTTTGAGAT910.14554411106135245No Hit
CGCTTAGGGGATAAAAAGAAAAGAAATTGGATGTATTTACAGCTGTCCTTGA910.14554411106135245No Hit
GCTGCGGGGAGACTTTGGAAGTGGCCTTGCCAGCGCTGTCCTGTCTTTCTGG910.14554411106135245No Hit
TCTAGGGGGTGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG910.14554411106135245No Hit
TCCAAAGGGAGACTAACAGTGGACTTCTCAGCAGAAACTTTACAAACCAGAA900.1439447252255134No Hit
TTATATGGGAGCTCATTTGTCCAAATTTCTGGGCTCAGCGCTTGGGAGGGCA900.1439447252255134No Hit
AATCCGGGATGAGAGAACTTGCCATGCTACGAAGACAGCAGATCAAGGTATT900.1439447252255134No Hit
GTATATGGGAGGATTATTTAAACTATCCCAAACCTGGCTCAAATCTCTTAGA900.1439447252255134No Hit
CTTTTAGGGAGCAGCAGCATCGGAGGTACCCCCGCCGTCGCAGCCCCCGCGC900.1439447252255134No Hit
ATCAACGGGCACTGAGATTATTCCTATAATTTTAAAGTATCATTTATTTGAA890.14234533938967436No Hit
ACCGCCGGGGAGAATCAAGCCAGCTGCAGAATTGGGGAGGAAGGGCTTTCCG890.14234533938967436No Hit
TACATAGGGAGCTCTGGAAGGCCTTGGATGTCAGATTAAAGGACTGAGATTT890.14234533938967436No Hit
GTCATAGGGGGAGCTGGGACGGGCGGCGGCCGGGTGGCCTCGGCCACCCGCT880.14074595355383532No Hit
AATCAAGGGTTAGCAAGAAAATGAAAGATATTCCTGAAAAATATTGCAAAAC870.13914656771799627No Hit
TGCTAAGGGAAATATTCACATGTAATAAAATAATGTTAGCATATAAATATAC870.13914656771799627No Hit
CTCCTAGGGATAACCAGCAACCATCAGAGCTACCAGCCCAAGCCATTCCATC860.13754718188215725No Hit
CTCGCTGGGTCTTCCGATCTTGAATAGGGAAGGCCCTCCATGGGCCTCTGCC860.13754718188215725No Hit
TCACATAGGATTTCATCTTAGAAAAAGTTGCTTATATTCAGCAGGTTGGTTT850.1359477960463182No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGT850.1359477960463182No Hit
TTGCCGGGGAAGTGCTTTATAGTTTACAGAGAGTTAAATATATATGTAGCAT850.1359477960463182No Hit
CTAACGGGGATGCCTTCACTACAACATTAGAATATTGTAACTCACATGCCTT840.13434841021047916No Hit
CCCAAAGGGATAGATAACCAAATGGAAACATATCCCATGCTCATGGATGGGA830.13274902437464012No Hit
TGCTATGGGATTTCCTAAATGGGATGCAAAAAGAAACTGAATGATGTTGAAT830.13274902437464012No Hit
TCACATGGGAACTAAGAACATTTTAAATGTACTTTCAAGTTTGTGTGACTAA830.13274902437464012No Hit
GTAGTAGGGCGATCTACGTGGGGGATCTGGAGGTGAGCAAAGTATGTTTGGT830.13274902437464012No Hit
GAAGTGGGGAGACATTAAAAGCATTGTTGGTGAGGACTTGAAAGGAAATGAG820.1311496385388011No Hit
GATCCGGGGGTTTTGTAGAAGTACTTTATTACATATATGAAATAAACTATAA820.1311496385388011No Hit
TAAATAGGGACTCCAGCCTGGGCAACAGGGCAAGAACCTGCCTCAAAAAAAG810.12955025270296208No Hit
GCCACTGGGCCCTCGTTTACCTCAGAGAAGTAAATTTGATAGTGATGAGGAA810.12955025270296208No Hit
TGTATCGGGATCTTACAGAAGTTGAGGCCAAGGGAGAATGGTAGGCACAGAA800.12795086686712304No Hit
ATGCTAGGGATCCTTTTATAGGCATTACCTTTGCAGGGGGCACAATTTTCAC800.12795086686712304No Hit
GGTAGCTTGGAGTCGAAGGGTGGTAGTACCCGAGCTATCAGCTGGTGTAGCC800.12795086686712304No Hit
TTAGATGGGATGTCAAGAGTTAAACCTCCTCAGGTTCAACCTGTGATAAAAG800.12795086686712304No Hit
ATAATGGGGCTGGGCTACGCATTTCTCCTTACCATGGCCACAACCAGTGACA790.126351481031284No Hit
TGTATGGGGATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAG790.126351481031284No Hit
TGCCGTGGGAGTCCTAGGCTCTGTTTGCTTATACTAAATGTCTCTTTATCCT790.126351481031284No Hit
CGACAAGGGATATATGCTCACTTCAATAAAAAGTTACTTATAAAAACAAACA790.126351481031284No Hit
TTGCATGGGTGCAGAGGGGGGCCACAGTGGGCAGGTGGCACCGGGGGTCAGC790.126351481031284No Hit
TAGCTGGGGACATCACTGAAGATGGCACTGGGAAGCGATACACACAGCCAGC780.12475209519544495No Hit
CGAGCAGGGAAGGAAAAAGGCATGCTTTCTGACTGACATGATCTTTTGCAAT780.12475209519544495No Hit
CTTGCAGCGGGAGCACCAGCGCCGGCTGCGTTCCGGGCCTCCGGTCGCCCGT770.12315270935960591No Hit
GTCAGTGGGGAAGGTCATCCTCAATGCCGCCACCTTAACAGGTGCCATGGAT770.12315270935960591No Hit
CGTGAGGGAATAAATAGCTTCGGTAAGGAGTTAATTTCCTTCTAGAAATCAG760.12155332352376687No Hit
ATGGCGGGATCTAAAAGCTGGGATAGTTCACCCCAAAAACTTTAAGTAGATC760.12155332352376687No Hit
ACAAGGAGGGATCACACTTGTAAAGTTTGGGACTTATAGACTTCTTGTTCTG760.12155332352376687No Hit
GTTATGGGGCAAGGTATTTGTTGCAACTTACTACATAGCATATACCCAAAAT760.12155332352376687No Hit
AATCTCGGGCGATCTAAAGACGGGCGTCTACCTGGTGTACGACCAGGAGCTG760.12155332352376687No Hit
CAGAGCGGGATGACCTCACAGAAGGTGGCTCATGTATTGAATGGGAATTTGG750.11995393768792784No Hit
ATGTCCGGGAGAAATGAAGTGGTTGTTATAAAAGGTTGAAATCACTAGGAAG750.11995393768792784No Hit
CACTATGGGTATACATCTTAATTATGGTGGAATTCCTATGTAGAGTCTAAAA750.11995393768792784No Hit
ATCTCCGGGAGTTTCATGTCCCTTAATAAATAATTAAGAAATCACTAAAATA750.11995393768792784No Hit
AAGAGTGGGATAATTTGAATCAACACAACCACCCACAGCCTAATTATTAGCA750.11995393768792784No Hit
GTTCTGGGGATTTGTATTAAACTTTTTCAATAAATATATACCAATTGATCCA750.11995393768792784No Hit
CCTGACCTTGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGG750.11995393768792784No Hit
GCATTAGGGATGAGAAGCGAGAGGGTGGCTGAGAGGCCTGGGCCTCTGGGAT740.11835455185208879No Hit
CTGACAGGGGAATCTAACATCAGGCTTGTTTTGTGCAGCCCATGAGCAAATA740.11835455185208879No Hit
TGCTAGGGGGGCTGCTGGCCTCACATTTCCACTGGCAGTGGAGCCTTTCCCT730.11675516601624976No Hit
CAGCTGGGGCCTGTTAAAAATGTAAAAGCCATTCTTAGCTTATAGCCCATAC730.11675516601624976No Hit
TGTCGCGGGAGTTGTTTTGGAAATTTTATGTAAAATTAAAACTATCAGTATT730.11675516601624976No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT730.11675516601624976No Hit
GAATCCGGGATGTGTCTTTATAGCAGCATGATTCATAGTCCTTTGGGTATAT730.11675516601624976No Hit
GTGCTAGGGACAGTGAAAATAGTTCTGAACGAGGACTTAAGGAGGGAAGTTG720.11515578018041071No Hit
TCGTAAGGGAATGATATTTATTATTGAATATTGATTTGCTTATATAAATGTG720.11515578018041071No Hit
ATGCCTGGGAGAGTTAAGCTACTAGACCTAGTGAAACAAAGAGGCTGAAATT720.11515578018041071No Hit
TTCGATGGGAGATGCCATTCCAGGTTAAACTGGGTTGTCTGTACTAAATTAT720.11515578018041071No Hit
AAATGGGGCGAGAGAAGGGGGTTCATCATGGCGGATGACCTAAAGCGATTCT710.11355639434457168No Hit
GATCGAGGGGGGGTCAGATAAAAAATGAATAAAGCAAGGATCTCTCTCTCTC710.11355639434457168No Hit
CTGTTTGGGATCATGGTGTAGTGTTCACTTACAAACATATCCTGTTGTTTCT710.11355639434457168No Hit
GATACTGGGTTATGCATTTATGCATGAGAACTAAATAGATTTTTAGATTCCT710.11355639434457168No Hit
GCATTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGCTGGGGC710.11355639434457168No Hit
GCTAAGGGGATGAGTGGAAGAATTTGGATGGCATTTCCCATGCACCTAATGC700.11195700850873264No Hit
ATATATGGGAAGAAGTCTGATTAAAAGTTGAAATCAGTATTTCTGAATTCAA700.11195700850873264No Hit
CTACGAGGGATAATGGTTTCAGTTAGTGAAGATGTTTATTTTAATAACTTTA700.11195700850873264No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTC700.11195700850873264No Hit
CCGAGGGGGAAGTGGGGAGAAAACTTAGGGTGCTTCTGTGAATTAAAGCTAA700.11195700850873264No Hit
ACGATGGGAACTATTAAGCATATGTGTAAGGGTTATGTATTTTAATAGCAAA690.11035762267289362No Hit
CCGAAGGGGCACCAGTGTTTGCACTAGTGAAATCTACCAACTCTTAAAGAAA690.11035762267289362No Hit
ATGACAGGGAGGCGAGGGCGCCGCACCCACACCGCGCTGCGCAGTTTTGTTC690.11035762267289362No Hit
ATACCAGGGCGATCTGGAGCTGGGATATTACTTTCCATTGGCCATACTTAGT690.11035762267289362No Hit
TGTAAAGGGATGAGCCCCAAGGCTGGGGCCACAGCTCCTACCCCTGCCCAGC690.11035762267289362No Hit
TTAGCAGGGAACCTCACTGAGTTAGAATCAGAGGTAAGTGGTATGCAGCTTA690.11035762267289362No Hit
CTAGTAGGGTACAGATACGTTAGCAGAGGCCTTTCAAAAAGGGAGTGTTTTT680.10875823683705457No Hit
CTTAATGGGTAGCGAAGGGAGCATGAAAGATTTAGTCAACTAATGAAAGGTT680.10875823683705457No Hit
ATAGATGGGAAGTTTAGACACATACATACAGATAGGCGTAGCAATAGAGATA680.10875823683705457No Hit
GTGCGAGGGGGGGAGAGTCTTCCACCCCTGGGGTGACTTCTGGGATGGTTGT680.10875823683705457No Hit
AATCAAGGGATGACAAGGAAACATCAGAATTACTAATCTAGCTAGTGTCATT670.10715885100121554No Hit
TCCACAGGGTGAGGAGGTACTGCTTGTAGCATATAGAGCCTCTCCCTAGCTT670.10715885100121554No Hit
GTCATTGGGAAGTCTATGTCATGAAGGGAAAATAAAAATGTATTCAGTAGCA670.10715885100121554No Hit
TTTACAGGGAAGGAGAAACAGCTTCAAAGGAGAGAGTTATTGGTGTGTGATT670.10715885100121554No Hit
TTGCCGAGGGATATCCTATTCAGAAAGCTGTGCATACAATTTATTTAAAGGA670.10715885100121554No Hit
ATTCTTGGGAGCAGCTCTCAAGCGTCACACTGCTCAGCTCCGCGAAGAGTTG670.10715885100121554No Hit
GGTGATGGGAAATCCAGGCTATTAAAGTCACTAAATTTCTAAGTATGTCCAT670.10715885100121554No Hit
CTGTTTAGGGTAGCTCCCACAGCTCCTTCAAGGGGCCGTGGGGGTGCCATTC670.10715885100121554No Hit
TCGATAGGGCCGATCTGTTGCTGGGAGCAGACTTTTAGTGGAAACCTTACAG670.10715885100121554No Hit
GGTTCTGGGAGGTTAATGATATGGTAAGGCCCCTAAAATAACTGAATTAATT670.10715885100121554No Hit
TGGACTGGGATGTCAGATCCGTCGATCCCTTCTGCGGCCACTCTGATCAACA670.10715885100121554No Hit
GGCATAGGGAGAAGCGGATTCGGTAGGGTTTTAAACGCCTCTGCAGAAAACT660.1055594651653765No Hit
ACCGTTGGGTCTGTAAGTGGGACTTGTGCAAATTGCAAAAAGCCTTTACAGA660.1055594651653765No Hit
TACACTGGGAGGCTGGAACGCACTGGTGTGATCACGGCTCACTGCAGCCTTG660.1055594651653765No Hit
AACAACGGGATGACTTCTAAAAATTTTTTCTTAAAATATATTAACAACATTA660.1055594651653765No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTCTTATA650.10396007932953746No Hit
AAGGTGGGATGACAGTAGCATACTGATGAGACAACACACACACACACAAAAC650.10396007932953746No Hit
TACCAAGGGAAGTGACTTCCTAGACATGAAGAAAAATTTAACATGCAATTCT650.10396007932953746No Hit
TACTAAGGGATCCGGCGCCGGCTTTCGGCGCGACGGTCGCCGCGTTCCATCG640.10236069349369842No Hit
CTACTAGGGATTCTTCCCTGGGCTGGTGAAGTACATGAACTCAGGGCCGGTT640.10236069349369842No Hit
GATTCTGGGACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGC640.10236069349369842No Hit
AATAACGGGGTTAAGTATCAGGAGATAACACCAGACACTGAAGAGAATGATA630.10076130765785939No Hit
CCTCTTGGGTATCTGAAGGACACAGTGGTGAATTCCTTGAATTTTCTCTTTT630.10076130765785939No Hit
GACTAGGGGATTGCCCCTCCTCAGCTGTGCAAGGAGAAAGCATGCTTAGGAA630.10076130765785939No Hit
GCCGCTGGGAGGAGCCCTGTTGGGGAAGTGGGAGATAGAGGAGATATTGGTT630.10076130765785939No Hit
GCATTGGGGTTCCGCACTTTCCACTTCTTCCTTTCTCTTTCTCTCTTCCCTC630.10076130765785939No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)