FastQCFastQC Report
Sat 31 Dec 2022
SRR5844521.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844521.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61734
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT17692.8655198108011795No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC15082.442738199371497No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC3670.5944860206693232No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA2980.48271616937182105No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2340.37904558266109434No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA2030.32883014222308615No Hit
TGACTGGGGGTGAGGACTGTTCCCATCCAGGCCCAGCTGGGGCTTTCCCTTT1830.29643308387598405No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1660.2688955842809473No Hit
TTGCATGGGCAACATATCCAAAATATTATATCAACAGGTAATTAATATAAAA1530.24783749635533095No Hit
TCGTCAGGGGCTCTCTCCTCCCGCCGCCCAAGATGCCGAAAGGAAAGAAGGC1470.2381183788512003No Hit
GCACTGGGGATATCCTGTGACTTAGAGTGTAGGATTTGTCATAAAAATCAGC1430.23163896718177987No Hit
GATCATGGGAGGAATTCAACATCTAGAAGTAATTCATAATATTTTTGGATAT1370.22191984967764927No Hit
ACAACCGGGCTGAAGACAGAGCAAAATGCTTTGAAAAGAATGAGGCCATACA1270.20572132050409825No Hit
CTGGGTGGGAGAATTCTTAAACTCTGTGATTTAGCTGTTTCAGTAAATAAAT1270.20572132050409825No Hit
TATTTGTGGGGTGACCAATAGCATCTCATGCATCCTAGAGCTTCCAGTCTAG1230.19924190883467782No Hit
TTAGCAGGGAACCTCACTGAGTTAGAATCAGAGGTAAGTGGTATGCAGCTTA1210.1960022029999676No Hit
AAATGTGGGAGTTGACAGCATATTAATTGTGTGGCATTATAATTGTGAGAAA1200.1943823500826125No Hit
TCACATGGGAACTAAGAACATTTTAAATGTACTTTCAAGTTTGTGTGACTAA1130.18304337966112677No Hit
TTATATGGGAGCTCATTTGTCCAAATTTCTGGGCTCAGCGCTTGGGAGGGCA1100.17818382090906146No Hit
TCCAAAGGGAGACTAACAGTGGACTTCTCAGCAGAAACTTTACAAACCAGAA1090.17656396799170634No Hit
GCTCCGGGGAGTTTCCATTTATGTCAATGCTAAATCCAAAGTCACTTCAGAG1070.17332426215699615No Hit
TGTCGCGGGATAGGATATTAGTACATCGTACACGTGCTAGGAAAAAACAGCT1070.17332426215699615No Hit
AAACATGGGAGCTCTCAAAATCCACTGAAGAAGTCAAGTGAAGGTGGGTAAA1070.17332426215699615No Hit
ACACACGGGATTCAACACAGTTTTCAACAGTGCTACTGAAAGCTACAGGAGA1070.17332426215699615No Hit
CTACGATGGGATTTCCCAGATTAAAAATACCAAAACTATTTTTTAAACTTAA1040.16846470340493083No Hit
GCATGCGGGAAGGTCTCATCTCACTCTCTTGCCCAGGCTAGAGTGCAGTGGC1030.16684485048757572No Hit
ACATGTGGGGATCAAATAGAATGGCTTTCTGGAATGACTAATTTGAAATATT1030.16684485048757572No Hit
CAAGCGGGGATGCCTTGAAAACCAGGAGAATATGGAATAAAACTTTTGAGAT1010.16360514465286552No Hit
ATCAACGGGCACTGAGATTATTCCTATAATTTTAAAGTATCATTTATTTGAA1000.1619852917355104No Hit
ATATATGGGAAGAAGTCTGATTAAAAGTTGAAATCAGTATTTCTGAATTCAA970.1571257329834451No Hit
CTCACTGGGATGAGAACTTCAGGAAAAAGCAGATTGAAGAGCTGAAGGGACA970.1571257329834451No Hit
CATAGCGGGGCCACCATGCCTGGCTAATTTTTTGTGTGTTTTTAGTACAGAC960.15550588006609No Hit
TTGAAAGGGACAGCCAGCCCAGCTCCACTCAGGGCTGGCCCGGGGAGTCCCC950.1538860271487349No Hit
AAAGCTGGGGAGATCAAAAATGGCGGTAGTTGGTGTGTCCTCGGTTTCTCGG950.1538860271487349No Hit
AGTCATGGGATGTACATAATGGAATACTATTCAGCCATAAAAAAGAATGAAA950.1538860271487349No Hit
TACATAGGGAGCTCTGGAAGGCCTTGGATGTCAGATTAAAGGACTGAGATTT950.1538860271487349No Hit
GCTGCGGGGAGACTTTGGAAGTGGCCTTGCCAGCGCTGTCCTGTCTTTCTGG950.1538860271487349No Hit
GCCACTGGGCCCTCGTTTACCTCAGAGAAGTAAATTTGATAGTGATGAGGAA950.1538860271487349No Hit
GATGCTGGGAGTTCTCAGCTGTTTACCACAGCCCTCCAGGGTCCAAAGATTG950.1538860271487349No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT940.15226617423137978No Hit
ATCTCCGGGAGTTTCATGTCCCTTAATAAATAATTAAGAAATCACTAAAATA940.15226617423137978No Hit
ACGTTTGGGATTCCATTTTCCTAGTGCCACATGTGAAATTGGATTTTGATGA940.15226617423137978No Hit
GCGCGGGGGCATCCGGCGACAGAGGACAGCCAGAAGCCCAGCCAGCCCCAGA930.15064632131402467No Hit
AATCCGGGATGAGAGAACTTGCCATGCTACGAAGACAGCAGATCAAGGTATT930.15064632131402467No Hit
CCCAAAGGGATAGATAACCAAATGGAAACATATCCCATGCTCATGGATGGGA910.1474066154793145No Hit
AAACGCGGGATGTACAGATGTACAAACATCATTTTAGGGTTTAATTTGTTTT910.1474066154793145No Hit
TGCTAAGGGAAATATTCACATGTAATAAAATAATGTTAGCATATAAATATAC910.1474066154793145No Hit
TCTAGGGGGTGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG900.14578676256195938No Hit
ATGCTAGGGATCCTTTTATAGGCATTACCTTTGCAGGGGGCACAATTTTCAC890.14416690964460427No Hit
TAAATAGGGACTCCAGCCTGGGCAACAGGGCAAGAACCTGCCTCAAAAAAAG890.14416690964460427No Hit
GTAGTAGGGCGATCTACGTGGGGGATCTGGAGGTGAGCAAAGTATGTTTGGT880.14254705672724916No Hit
GAAGTGGGGAGACATTAAAAGCATTGTTGGTGAGGACTTGAAAGGAAATGAG870.14092720380989407No Hit
TTTGGTAGGGATTAGAAAGTCTGCAAATTAGGTTGTTTGCTTTGTGGTGAGG870.14092720380989407No Hit
TGTATGGGGATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAG870.14092720380989407No Hit
AATCAAGGGATGACAAGGAAACATCAGAATTACTAATCTAGCTAGTGTCATT860.13930735089253896No Hit
ATGGCGGGATCTAAAAGCTGGGATAGTTCACCCCAAAAACTTTAAGTAGATC860.13930735089253896No Hit
GGTAGCTTGGAGTCGAAGGGTGGTAGTACCCGAGCTATCAGCTGGTGTAGCC860.13930735089253896No Hit
AATCTCGGGCGATCTAAAGACGGGCGTCTACCTGGTGTACGACCAGGAGCTG860.13930735089253896No Hit
GGTGATGGGAAATCCAGGCTATTAAAGTCACTAAATTTCTAAGTATGTCCAT850.13768749797518387No Hit
TAGCTGGGGACATCACTGAAGATGGCACTGGGAAGCGATACACACAGCCAGC850.13768749797518387No Hit
CTTTTAGGGAGCAGCAGCATCGGAGGTACCCCCGCCGTCGCAGCCCCCGCGC850.13768749797518387No Hit
TTAGATGGGATGTCAAGAGTTAAACCTCCTCAGGTTCAACCTGTGATAAAAG850.13768749797518387No Hit
GCTCGGGGACGTGCGCCACCACACCCAGTTCATTTTTGTATTTTTAGTAGAG830.13444779214047364No Hit
TGGCTGGGATTGTTCTTCAAGCATGTTTTAGACCTAGAAAAGTATGGTTTGG830.13444779214047364No Hit
TAAGGTGGGAAGACATGGTTATTGACCTACTGCTTGACCAACTGACAGACCA830.13444779214047364No Hit
GCTGTAGGGATGTCTTTGTTATCTTATTACAAGAATTGTTATTTGAAAAAGA830.13444779214047364No Hit
ATGTCCGGGAGAAATGAAGTGGTTGTTATAAAAGGTTGAAATCACTAGGAAG820.13282793922311853No Hit
CCTGACCTTGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGG820.13282793922311853No Hit
ACCGCCGGGGAGAATCAAGCCAGCTGCAGAATTGGGGAGGAAGGGCTTTCCG820.13282793922311853No Hit
TGGCCGGGGCAAGATGTGCTTCTCTGCAATAACCAAAGACCTGAGCATGTCA810.13120808630576344No Hit
TAAAAGGGGGAGCATGGGCCTTGGGAGCCATTTGGAGGCTGGCTACCTCATG810.13120808630576344No Hit
GATCGAGGGGGGGTCAGATAAAAAATGAATAAAGCAAGGATCTCTCTCTCTC800.12958823338840833No Hit
TTTATAGGGCGATCTAATCAGGGGACATTTACATCTTTAATCCATCTGGAAT800.12958823338840833No Hit
TATCACGGGGTGTGGTAAAATTGGGGTAAAATGTGGTAAAATGTGATACGCA800.12958823338840833No Hit
TTGCCGGGGAAGTGCTTTATAGTTTACAGAGAGTTAAATATATATGTAGCAT800.12958823338840833No Hit
GGTTCTGGGAGGTTAATGATATGGTAAGGCCCCTAAAATAACTGAATTAATT800.12958823338840833No Hit
TTGCATGGGTGCAGAGGGGGGCCACAGTGGGCAGGTGGCACCGGGGGTCAGC800.12958823338840833No Hit
GTCATTGGGAAGTCTATGTCATGAAGGGAAAATAAAAATGTATTCAGTAGCA790.12796838047105324No Hit
TGTCGCGGGAGTTGTTTTGGAAATTTTATGTAAAATTAAAACTATCAGTATT790.12796838047105324No Hit
GTGCTAGGGACAGTGAAAATAGTTCTGAACGAGGACTTAAGGAGGGAAGTTG790.12796838047105324No Hit
GATCCGGGGGTTTTGTAGAAGTACTTTATTACATATATGAAATAAACTATAA790.12796838047105324No Hit
GATACTGGGTTATGCATTTATGCATGAGAACTAAATAGATTTTTAGATTCCT790.12796838047105324No Hit
ATAATGGGGCTGGGCTACGCATTTCTCCTTACCATGGCCACAACCAGTGACA790.12796838047105324No Hit
GTGGACGGGAACCGACTAATCACCACCCAACAATGACTAATCAAACTAACCT790.12796838047105324No Hit
TGAGAGGGTAACATTAATTAACTTTCTAGCATTAAATAAACTTGCTTTTCTG790.12796838047105324No Hit
GTCATAGGGGGAGCTGGGACGGGCGGCGGCCGGGTGGCCTCGGCCACCCGCT780.12634852755369813No Hit
GGCCAAGGGATCTGACTGAGAAAATAGTTATTTCAGAGATAAAATAAGGAAA780.12634852755369813No Hit
AAGGCTGGGAATGTTGGGGAAAGTGAATTTTTCTCCTTTGTAGAAGGAGGTA780.12634852755369813No Hit
TCACATAGGATTTCATCTTAGAAAAAGTTGCTTATATTCAGCAGGTTGGTTT780.12634852755369813No Hit
AATCAAGGGTTAGCAAGAAAATGAAAGATATTCCTGAAAAATATTGCAAAAC780.12634852755369813No Hit
GTTCTGGGGATTTGTATTAAACTTTTTCAATAAATATATACCAATTGATCCA780.12634852755369813No Hit
GTACGCGGGGGGACCGGCGCTGGGCAGCCAGGACAGCCGCGGCAGCCGGGTC780.12634852755369813No Hit
CAGAGCGGGATGACCTCACAGAAGGTGGCTCATGTATTGAATGGGAATTTGG770.12472867463634303No Hit
AAGGTGGGATGACAGTAGCATACTGATGAGACAACACACACACACACAAAAC770.12472867463634303No Hit
AAATATGGGGAAGACTGTCATAGGTAACTTTGGTTTCTTTCATACTGGGGAT770.12472867463634303No Hit
CGCTTAGGGGATAAAAAGAAAAGAAATTGGATGTATTTACAGCTGTCCTTGA770.12472867463634303No Hit
CGACAAGGGATATATGCTCACTTCAATAAAAAGTTACTTATAAAAACAAACA770.12472867463634303No Hit
ATTCTTGGGAGCAGCTCTCAAGCGTCACACTGCTCAGCTCCGCGAAGAGTTG760.12310882171898792No Hit
AACTTAGGGAGGAGAGGAAAAGGTGTAGGCCTGCCTTACCGAGAGCCAAACA760.12310882171898792No Hit
TTGCCGAGGGATATCCTATTCAGAAAGCTGTGCATACAATTTATTTAAAGGA750.12148896880163282No Hit
ACGGTTGGGGACGCTCTTCCGATCTACTGCTGGGGGAACAAGAATTACAGAT750.12148896880163282No Hit
GTTATGGGGCAAGGTATTTGTTGCAACTTACTACATAGCATATACCCAAAAT750.12148896880163282No Hit
AACGGGAAGGCCAGCTACAGGTGGACTCCTGGAATTTGAGGCATCATAATGA740.1198691158842777No Hit
ATGCCTGGGAGAGTTAAGCTACTAGACCTAGTGAAACAAAGAGGCTGAAATT740.1198691158842777No Hit
GTGATAGGGGAACTTTCTAGTTGTGTACTTAATTTTGATGTGGAAGCAGTAA730.1182492629669226No Hit
GCAATGGGGCAGAGAAAAGCTCAGAATTTGACGGCATCACTGTTGAATTCTA730.1182492629669226No Hit
TCACATGGGTCTCTGTGATTTTTCAATATTTTTAAATGTTTCCAAATCTTTA720.11662941004956749No Hit
CGCTATGGGATGTGCATCTCTGTCAGCCTGAGTGGCTTTGTGGTCTTGGGCT720.11662941004956749No Hit
GATCCAGGGCTGGCATTGGGGAGACATCCAGATTTTCTCCTCTATCCTATGC710.1150095571322124No Hit
CTACGAGGGATAATGGTTTCAGTTAGTGAAGATGTTTATTTTAATAACTTTA710.1150095571322124No Hit
AACTGGGGGGGTTTGCTTACGGTTCTGGTTTCCTACTCAGTGTCCGTGACCC710.1150095571322124No Hit
CGGCTTGGGAACAAAAAGCTGCTGCATGCCAAGTCACTGTGCTAGGCATTGA710.1150095571322124No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTC700.11338970421485728No Hit
AAGAGTGGGATAATTTGAATCAACACAACCACCCACAGCCTAATTATTAGCA700.11338970421485728No Hit
ATCAAAGGGAGAGGGAGCTGTGGTAAGGGTTGGGCCCCCAGCCTGTAGGGAA700.11338970421485728No Hit
TCGATAGGGCCGATCTGTTGCTGGGAGCAGACTTTTAGTGGAAACCTTACAG690.11176985129750219No Hit
AATCAGGGGGACGCTCTTCCGATCTAACCTAGGGAACCCCCTAGGAATCACC690.11176985129750219No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTCTTATA680.11014999838014708No Hit
CTTGCAGCGGGAGCACCAGCGCCGGCTGCGTTCCGGGCCTCCGGTCGCCCGT680.11014999838014708No Hit
ACGATGGGAACTATTAAGCATATGTGTAAGGGTTATGTATTTTAATAGCAAA680.11014999838014708No Hit
CCGCTCGGGAGTGTGGGCCCTCAGGCAGCCCTGAAGCCTGGCTGGGTCATCA680.11014999838014708No Hit
TGTATCGGGATCTTACAGAAGTTGAGGCCAAGGGAGAATGGTAGGCACAGAA680.11014999838014708No Hit
CTTAATGGGTAGCGAAGGGAGCATGAAAGATTTAGTCAACTAATGAAAGGTT680.11014999838014708No Hit
TACTAAGGGATCCGGCGCCGGCTTTCGGCGCGACGGTCGCCGCGTTCCATCG680.11014999838014708No Hit
GTAATAGGGATGTCTTACTAACTTCCTATACTCTGATCCGGCTCAAAATATC680.11014999838014708No Hit
GGCTATGGGCAGGTATCTCATTGAATCAGTGCGTCAGAGAGATTCTAAGATT680.11014999838014708No Hit
ACTTTGGGATATTCTTAAATAAACTAGCCTATTTAGAAATTGGTAAAATTTA670.10853014546279198No Hit
GCATTAGGGATGAGAAGCGAGAGGGTGGCTGAGAGGCCTGGGCCTCTGGGAT670.10853014546279198No Hit
TGCTAGGGGGGCTGCTGGCCTCACATTTCCACTGGCAGTGGAGCCTTTCCCT670.10853014546279198No Hit
CAGCTGGGGCCTGTTAAAAATGTAAAAGCCATTCTTAGCTTATAGCCCATAC670.10853014546279198No Hit
GACTAGGGGATTGCCCCTCCTCAGCTGTGCAAGGAGAAAGCATGCTTAGGAA670.10853014546279198No Hit
GATTCTGGGACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGC670.10853014546279198No Hit
ATGACAGGGAGGCGAGGGCGCCGCACCCACACCGCGCTGCGCAGTTTTGTTC660.10691029254543687No Hit
TTTACAGGGAAGGAGAAACAGCTTCAAAGGAGAGAGTTATTGGTGTGTGATT660.10691029254543687No Hit
CGTAAAGGGATCGAATACATGCCTGGCTTCTCCTTCGCCTTCCACCATGATT660.10691029254543687No Hit
TGCCGTGGGAGTCCTAGGCTCTGTTTGCTTATACTAAATGTCTCTTTATCCT660.10691029254543687No Hit
CTTAATGGGTTACAGAAATGAGTTGTCACTTCCTTGTTGTTACTTATTTCTG650.10529043962808178No Hit
GCCGCTGGGAGGAGCCCTGTTGGGGAAGTGGGAGATAGAGGAGATATTGGTT650.10529043962808178No Hit
AAATGGGGCGAGAGAAGGGGGTTCATCATGGCGGATGACCTAAAGCGATTCT640.10367058671072665No Hit
ATAGATGGGAAGTTTAGACACATACATACAGATAGGCGTAGCAATAGAGATA640.10367058671072665No Hit
CTAACGGGGATGCCTTCACTACAACATTAGAATATTGTAACTCACATGCCTT640.10367058671072665No Hit
GCGATAGGGATAGTAAGCAAGTTTGTGTTGAATTTAGCCATATGCTTAGCTG640.10367058671072665No Hit
TCAGCTGGGCATGTGTAATTTCACATCAATAACTTGTGTTTCTGAAATATCC630.10205073379337157No Hit
CGTGCGGGGGGGTTGTTGAAAACTAATTGTATTAAATGTCTTTTGATAAGCC630.10205073379337157No Hit
CTGTTTGGGATCATGGTGTAGTGTTCACTTACAAACATATCCTGTTGTTTCT630.10205073379337157No Hit
CGCTGGGGGATAGTGATAAATCTCTTATTAATAACCCGATTAATTTTTGTGA630.10205073379337157No Hit
CTGTTTAGGGTAGCTCCCACAGCTCCTTCAAGGGGCCGTGGGGGTGCCATTC630.10205073379337157No Hit
CGAAGCGGGACATATGTTTATTGCAGCACTATTCACAATAGCAAAGACTTGG630.10205073379337157No Hit
CGTGAGGGAATAAATAGCTTCGGTAAGGAGTTAATTTCCTTCTAGAAATCAG620.10043088087601645No Hit
CTTAAGGGGTATTATCAGCCCCATTTTATAGATAGACCCTAAGGCTTTGAGG620.10043088087601645No Hit
TACCAAGGGAAGTGACTTCCTAGACATGAAGAAAAATTTAACATGCAATTCT620.10043088087601645No Hit
TACACTGGGAGGCTGGAACGCACTGGTGTGATCACGGCTCACTGCAGCCTTG620.10043088087601645No Hit
TCGTAAGGGAATGATATTTATTATTGAATATTGATTTGCTTATATAAATGTG620.10043088087601645No Hit
TTTTGTGGGAAGCTTCACAAATGCTCATGTATAGGCCGGGCGCCGTGGCTCA620.10043088087601645No Hit
ATCCTCGGGTTTAGGAGTAAAGTTTGAAGAGATAAAAATAGCCAAAGATAGG620.10043088087601645No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)