FastQCFastQC Report
Sat 31 Dec 2022
SRR5844519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37071
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT16704.504869035094818No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC15024.051684605217016No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA2770.7472148040247093No Hit
ACGCGCCTGGCTCTGCCACTGTTCTCTTCCCTCTGCTGCAAAGCCCAGTTAA2750.7418197512880688No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2160.5826656955571741No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC2140.5772706428205335No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA2070.5583879582422918No Hit
TCCGTGGGGAAGGAGAACTTCAGTTGCTTGACTCGTTTGGATCACAACCGAG1990.5368077472957298No Hit
TGGTGGGCTAGGCTCACTAAACATTCTACTACTCACTCTCACTGCCCAAGAA1970.5314126945590893No Hit
TCAGCTGGGAGTGTCATCTATTGCGTGATTTTTATATAAATGGAATCATATG1810.48825227266596527No Hit
GTAATTGGGATAAACAATGGTCACGTTGTGATAGGGATAACAATAAATCTAT1770.4774621671926843No Hit
GACTGAGAGTGCCCCCACCCATCTCATGCCCCAGGGACCGCCACTGCCCACC1660.4477893771411615No Hit
ACAGTAGGGATCTAGTGTTGGGATGTAATAAAGTCAGACAAAATGCATACTT1540.41541906072131857No Hit
AGCGCTGGGAATGCTTTGCTGGTGCTCTGGCATTTTGTCTTTATCTCATCAC1540.41541906072131857No Hit
GATATAGGGGATAACTCTATTATATGAATTCTTGTGAGTTATTTTATTGTAG1530.4127215343529983No Hit
CTACCGAGGGGGAATAATATTCAGCAATAAAAAGGAACAAAGTATTCATATA1410.3803512179331553No Hit
CATATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1350.3641660597232338No Hit
AGCATGAAGGGATAATAAATGAAAAAATAGTCTTACTGATTGCAAATTGATA1300.3506784278816325No Hit
TGTGACGGGAGTACTATTCAGCCATAAAAAAGAATGAGATCCTGTCATTTGC1280.345283375144992No Hit
AAATATGGGAGGATGGTAGTGGCACCCTCAGAGCCCCATCTTCAGCATGTTC1220.3290982169350705No Hit
GATCTTGGGCACGGGGAATGAGTGAAATAACCAGCAAGCAAACACCTGTCAG1190.3210056378301098No Hit
AACTTTGGGTTATACCACATATCGCATATGACCATCTTTCCATCAAATCAAT1130.30482047962018827No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1120.30212295325186805No Hit
ATGCCAGGGGCGGCGTCGCCAGGAGGAGCGCGCGGGCACAGGGTGCCGCTGA1110.2994254268835478No Hit
GTTTCCGGGAGACTAATAAAGAAGAAAGACAGAAGAATCAAATAGACACAAT1100.29672790051522757No Hit
TAATCTGGGGGTATAATTGGGATTGAAAACTAAAACTTGGAACTAAGATAGA1100.29672790051522757No Hit
CACGCTGGGACCAGACTGCCAGACGACTACATCATTCTGCCCACAGACCTGC1100.29672790051522757No Hit
CTTCCTGGGCGAACTGCTGGGATTACAGGTGTGAACCATTGTGCCCAACCCT1100.29672790051522757No Hit
ATTATAGGGACTGTCATAGAAAAACTGAATTCTCGTAAGATATTGTTGTTTC1080.29133284777858703No Hit
AACAATGGGATTTAAGGATAAAGGTAAATCATTCAAGGCAGTTACCAACCAC1060.28593779504194655No Hit
GTAAATGGGCTTTGCTGGGCCTCCGCAAGGGCCTCTCTTGGGTCTGTGTCCT1000.269752636832025No Hit
GTAATAGGGTGTGTTTTTTGGTAAAATGGTGATTGTAGATAAGATTAGTTCC990.2670551104637048No Hit
CATAACGGGGGATCCCCAAAGGATGTCTATTTTTGGCCACTCCATGGGAGGT980.2643575840953845No Hit
ATAACGGGGGAAGCAAAGGTTTATTTTTTGGTGTCCAGATTATCCATAGCTC940.25356747862210355No Hit
GCTTAGGGGATGTCTCTTTTAAGCAAATGTTTTGATGCATATCTTTTTAATT930.2508699522537833No Hit
TTCGATGGGTGCTGGGACAGAGTCCTGTAATCAGTGCATGTCCTTAGTCTGT930.2508699522537833No Hit
TGAATAGGGGAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCAT920.24817242588546304No Hit
ATGCTCGGGCAAGATCACATCACATTTGTAAAAATGGTAAGTTAATGCATTT870.23468479404386175No Hit
CTTTCGGAGGGGAGACAAAGATGGCCTCATGTACTGGTACCAATTGGATCAA850.22928974130722127No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT830.22389468857058076No Hit
TGTAGTGGGATTGAATGTTTAATATATGATAAAAATCAGTACTAGTATTATA830.22389468857058076No Hit
CAGTTAGGGGCGTGTTTAATGATGATGACTCCGCAAGCCCTCTGACATTGTG830.22389468857058076No Hit
TTTACTTGGGGGAGTCAGTCATCCAATTTTTCATGACAGAAAAACAACATTT820.22119716220226054No Hit
TAACTGGGGACTGGAGCCCATACCTAGGAAAGGAGGCAAGGATGTATTCCAG790.21310458309729977No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGT780.21040705672897955No Hit
AGCAATGGGAAGCTCGGGGCCATGCCGTGCCACCGTCACCAGAGCCAGCTGC770.20770953036065928No Hit
GACATGGGGACTGAAACATGCTTTAAATCGTGTCTAAAATCTGATTTGAGCA750.20231447762401877No Hit
GTTTCAGGGAATGTTGCGTCATTAAAGGTCAAGGGGTCATGCTATTAATCTC750.20231447762401877No Hit
ACTTTTGGGAGGATGGCAATTGCTCAGACTTGTCCTTTGCTTGTCTGGTTAT740.1996169512556985No Hit
TATAACGGGATAATGATAAAGAGTTCAATTCAACAAGAAGACTTAACTATCC690.18612931941409727No Hit
TACTAGTGGGAGCCCACCAGGGTCCAGGGAGATGTTCGTTCTCGCTTTAAGT660.17803674030913652No Hit
ACACCGGGGAGGTCAGCCATATCCAAAAGACCACAAGTCATTACTAAGTTGA650.17533921394081625No Hit
ATAGCAGGGAACAGTCGTATGATTTCTAGCCCATGGGTTTAAAACTGTATCT640.172641687572496No Hit
TTCATGGGGCAAGAAGTATAATATGCGTGTGGAAGACTACGAACCTTACCCG630.16994416120417577No Hit
TACTTGGGACAGAAGAGAACTCCTAGAAGAGCTTTACTTAACTATGAAAATT630.16994416120417577No Hit
ATAAATGGGGGGAAGAAGGAAGGAAGAGTAAATATTAACAAACATGATTTTT620.16724663483585553No Hit
TTTATAGGGTACATCTATTGGACAAGTTTTAATTAATTCTTTCCCTTGGCTG620.16724663483585553No Hit
GTAGTGGGGACCTCCCACAAGGATTACCCCTTCCATGAAGAGTTTTGAAGCT610.16454910846753526No Hit
TGAATGGGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTC610.16454910846753526No Hit
AGTTCTGGGACATTTTCAAGTTTATTAGGTTAAGAAAAACAGGGCAGCCTTG600.16185158209921502No Hit
TAAGCCGGGAGCCCTCACAGATGCCCAGCCTCATAGCTGAAGTTGCCTGAAT600.16185158209921502No Hit
TTCCAAGGGACAGTCCTTCAGCAGATTGATTCAGTGGCTAATGCTGATATCA600.16185158209921502No Hit
GTTAATGGGATACAAACTGATGTTGACTTTTGTGAGATATATCTTCTGTATC590.15915405573089478No Hit
GAGAATGGGGCAGCCTTCCTCTTCTGGGACTGCTGTGAGGCAGAGGAATGAT590.15915405573089478No Hit
GTTATGGGGAGCCTTGGGAAGCATTTCCATCTATCGGGACACCCACCAGTCG580.1564565293625745No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTC580.1564565293625745No Hit
AGGCACGGGAGAAATAGCTTGCTATACTGAAGGATGGAGGTAAGGACTGGTA580.1564565293625745No Hit
GCGTAAGGGCAGAAAAGTAGTGAACTTATTTTCAGTAGACATAGAAAACAAT580.1564565293625745No Hit
GTGCTTGGGGTAAACCAAAAGATGGAAGACAAAAAGACAAACACACCCACAC570.15375900299425427No Hit
GCAGTTGGGAGAGACCAACCGAACCCCCTTCGACCTTGCCGAAGGGGAGTCC560.15106147662593403No Hit
ACTAATAGGCTCTCTGTTGATATTTAAAGAAGTGAGGGCTGTGGGTATTCAT560.15106147662593403No Hit
GATTTTGGGCTGCCACTCTTAACACTAAGTGAGAGTTGGGGAGAAACTGAAG550.14836395025761379No Hit
TATCCGGGGGCGCATGCTCCGTTGTCCCTTCAGACTTCACTTATAACACACA540.14566642388929352No Hit
ACGTTTGGGGTAACTAAAGTTACTGGCTTGGTCTTATTTGCACAGTTTTTGC540.14566642388929352No Hit
CTATCAGGGCAACTCCTTGAGGGTAAAAGGGAACTCCTCCTTTTTGAAAGCT540.14566642388929352No Hit
CTTAATGGGAATTCTATATTTTCTGGCAAGTTTCTATACGAAGTATGATCCA530.14296889752097328No Hit
CTCTTTTTCTCAGTAAATCATATCATCTGAAATATTACAAATTTCAAATTTC530.14296889752097328No Hit
TCTGCCTGGGAGGGGCTTTGGGGACATGGCCTGTCCCCACATCCAGCACTTG520.14027137115265303No Hit
AGAACAGGGGAGAGGAGTTCCAGCTGTTCTGGCCACACTGGGCTGGTGGGGA520.14027137115265303No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTCTTATA510.13757384478433277No Hit
CATTTTGGGGATTTGTATGATCTTATAAAATGAAATAAGCAGACTTAAAAAA510.13757384478433277No Hit
ACTAAAGGGGATTTTTAGAACCTTTGCCTATATTAGGTTGTACTTATGTACA500.1348763184160125No Hit
TCAATGCGGGATCATTTAATAATATACTTGTGGACTAAAAGATATAAGTGCT500.1348763184160125No Hit
ATTATAGGGCAGAGACTTGACTGCCAGTTGAGTAAAGGTGCAAGATATGAGG500.1348763184160125No Hit
AACTTCGGGTGAGGGACATGGGGGTACAAAGTAGGCTTTTTAACTTTATAAA500.1348763184160125No Hit
GGCTATGGGGGGTGCGGAATCAAATAGGAAGCATGCAGAGACTCGGCAGCTT480.12948126567937202No Hit
GGTAGTGGGATCAACGGGATATCAAAATGATTTTGAGGATAGTAATTTGAGG480.12948126567937202No Hit
TAGTGTGGGGTCCGCCAGACCGCCGCCGCGCCGCCATCATGGACACCAGCCG480.12948126567937202No Hit
ATTTCAGGGGTGTAAACCTCAGGGTTAAAACAACCCTGTTATATAGGGATAA470.12678373931105177No Hit
TCATCAGGGCATGGCCCACATGGCCTCCAAGGAGTAAGACCCCTGGACCACC460.12408621294273152No Hit
TACGGAGGGTGGCCATGAAGGACCTTTTGACCAACTCCCTGTCATTCCTAAC450.12138868657441126No Hit
ATTGATGGGGACAATTGAGCAATTACCACAGAGATTAGCAATGGAGTTCCTA450.12138868657441126No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTAT450.12138868657441126No Hit
TAATGCGGGGCTTTTGAAGTAGTGTTCTGTTTAAACTGGCGCTCTGGTCTAT440.11869116020609101No Hit
AATAACAGGGATAGAAAGAATATGTGTCTTTTTCACATTTGAATATCTATGG440.11869116020609101No Hit
AAGCAAGGGGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAA430.11599363383777077No Hit
GGCATGGGGGTCCGGGGCCCTGGGTCAGAGGGGCCGGCGGACGAGGCCTCGG420.11329610746945051No Hit
GTTTTGGGAAAGACTACAGTCATACCTCAACTATCTACAGAGGACTGGTTCC410.11059858110113027No Hit
TAAAAAGGGTAAGAGATCAAGCCGGAGAGCTGGTAAAGCTGAACAAACAGGG410.11059858110113027No Hit
CAGCATTTTGGGAGACTGAGGCGGGCTGATCACTTGAGGTCAGGAGATCGAG410.11059858110113027No Hit
CAGTTAGGGGGCAGAGGACGATAAGCACTGCTTCCCGCAGGCATTTTAAAAA390.10520352836448978No Hit
ATCCCAAGAATCCATCAGCAACCTCAGACCAACCCAAGAAGATAATTTAAAT390.10520352836448978No Hit
GCTACTGGGAGCCACAGAAAAACAGGCAGTCAATTTTTAAAGGACTTCAGCT390.10520352836448978No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCC380.10250600199616952No Hit
GTGATTGGGGTTACTTTTCTAGCAGATTTTAACAAATAATCAAGGACATTTT380.10250600199616952No Hit
GTCTATGGGGTGGCCTGGTTGGTACATGCCGTCATGATGAAGGATGACTTTG380.10250600199616952No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)