FastQCFastQC Report
Sat 31 Dec 2022
SRR5844518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37027
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT16274.394090798606422No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC14713.9727766224646883No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA2880.777810786723202No Hit
ACGCGCCTGGCTCTGCCACTGTTCTCTTCCCTCTGCTGCAAAGCCCAGTTAA2600.7021902935695573No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2260.610365409025846No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC2070.5590515029573014No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA1930.5212412563804791No Hit
CTACCGAGGGGGAATAATATTCAGCAATAAAAAGGAACAAAGTATTCATATA1860.5023361330920679No Hit
TCCGTGGGGAAGGAGAACTTCAGTTGCTTGACTCGTTTGGATCACAACCGAG1810.4888324736003457No Hit
AGCGCTGGGAATGCTTTGCTGGTGCTCTGGCATTTTGTCTTTATCTCATCAC1810.4888324736003457No Hit
GTAATTGGGATAAACAATGGTCACGTTGTGATAGGGATAACAATAAATCTAT1810.4888324736003457No Hit
ACAGTAGGGATCTAGTGTTGGGATGTAATAAAGTCAGACAAAATGCATACTT1790.48343100980365683No Hit
TGTGACGGGAGTACTATTCAGCCATAAAAAAGAATGAGATCCTGTCATTTGC1790.48343100980365683No Hit
TCAGCTGGGAGTGTCATCTATTGCGTGATTTTTATATAAATGGAATCATATG1610.4348178356334566No Hit
GACTGAGAGTGCCCCCACCCATCTCATGCCCCAGGGACCGCCACTGCCCACC1450.3916061252599455No Hit
TGGTGGGCTAGGCTCACTAAACATTCTACTACTCACTCTCACTGCCCAAGAA1430.38620466146325655No Hit
AGCATGAAGGGATAATAAATGAAAAAATAGTCTTACTGATTGCAAATTGATA1410.3808031976665676No Hit
GATATAGGGGATAACTCTATTATATGAATTCTTGTGAGTTATTTTATTGTAG1400.3781024657682232No Hit
TAATCTGGGGGTATAATTGGGATTGAAAACTAAAACTTGGAACTAAGATAGA1360.36729953817484534No Hit
GTTTCCGGGAGACTAATAAAGAAGAAAGACAGAAGAATCAAATAGACACAAT1330.35919734247981205No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1330.35919734247981205No Hit
CATATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1330.35919734247981205No Hit
GATCTTGGGCACGGGGAATGAGTGAAATAACCAGCAAGCAAACACCTGTCAG1320.3564966105814676No Hit
CATAACGGGGGATCCCCAAAGGATGTCTATTTTTGGCCACTCCATGGGAGGT1150.3105841683096119No Hit
AAATATGGGAGGATGGTAGTGGCACCCTCAGAGCCCCATCTTCAGCATGTTC1130.305182704512923No Hit
AACTTTGGGTTATACCACATATCGCATATGACCATCTTTCCATCAAATCAAT1100.29708050881788967No Hit
CACGCTGGGACCAGACTGCCAGACGACTACATCATTCTGCCCACAGACCTGC1090.2943797769195452No Hit
ATAACGGGGGAAGCAAAGGTTTATTTTTTGGTGTCCAGATTATCCATAGCTC1070.2889783131228563No Hit
CTTCCTGGGCGAACTGCTGGGATTACAGGTGTGAACCATTGTGCCCAACCCT1050.2835768493261674No Hit
AACAATGGGATTTAAGGATAAAGGTAAATCATTCAAGGCAGTTACCAACCAC1040.2808761174278229No Hit
TTCGATGGGTGCTGGGACAGAGTCCTGTAATCAGTGCATGTCCTTAGTCTGT1030.27817538552947846No Hit
CTTTCGGAGGGGAGACAAAGATGGCCTCATGTACTGGTACCAATTGGATCAA990.2673724579361007No Hit
GTAATAGGGTGTGTTTTTTGGTAAAATGGTGATTGTAGATAAGATTAGTTCC970.2619709941394118No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGT970.2619709941394118No Hit
GTAAATGGGCTTTGCTGGGCCTCCGCAAGGGCCTCTCTTGGGTCTGTGTCCT970.2619709941394118No Hit
TAACTGGGGACTGGAGCCCATACCTAGGAAAGGAGGCAAGGATGTATTCCAG960.25927026224106736No Hit
ATGCTCGGGCAAGATCACATCACATTTGTAAAAATGGTAAGTTAATGCATTT950.2565695303427229No Hit
TTTATAGGGTACATCTATTGGACAAGTTTTAATTAATTCTTTCCCTTGGCTG910.2457666027493451No Hit
GTTTCAGGGAATGTTGCGTCATTAAAGGTCAAGGGGTCATGCTATTAATCTC900.24306587085100062No Hit
CAGTTAGGGGCGTGTTTAATGATGATGACTCCGCAAGCCCTCTGACATTGTG890.24036513895265618No Hit
GCTTAGGGGATGTCTCTTTTAAGCAAATGTTTTGATGCATATCTTTTTAATT870.23496367515596725No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT850.22956221135927837No Hit
ATGCCAGGGGCGGCGTCGCCAGGAGGAGCGCGCGGGCACAGGGTGCCGCTGA850.22956221135927837No Hit
ATTATAGGGACTGTCATAGAAAAACTGAATTCTCGTAAGATATTGTTGTTTC840.22686147946093393No Hit
TGAATAGGGGAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCAT820.221460015664245No Hit
TACGGAGGGTGGCCATGAAGGACCTTTTGACCAACTCCCTGTCATTCCTAAC820.221460015664245No Hit
TCAATGCGGGATCATTTAATAATATACTTGTGGACTAAAAGATATAAGTGCT790.21335781996921166No Hit
AGCAATGGGAAGCTCGGGGCCATGCCGTGCCACCGTCACCAGAGCCAGCTGC790.21335781996921166No Hit
TATAACGGGATAATGATAAAGAGTTCAATTCAACAAGAAGACTTAACTATCC780.21065708807086722No Hit
AGGCACGGGAGAAATAGCTTGCTATACTGAAGGATGGAGGTAAGGACTGGTA760.2052556242741783No Hit
TGTAGTGGGATTGAATGTTTAATATATGATAAAAATCAGTACTAGTATTATA750.20255489237583382No Hit
TCTGCCTGGGAGGGGCTTTGGGGACATGGCCTGTCCCCACATCCAGCACTTG740.1998541604774894No Hit
AACTTCGGGTGAGGGACATGGGGGTACAAAGTAGGCTTTTTAACTTTATAAA720.1944526966808005No Hit
ATTTCAGGGGTGTAAACCTCAGGGTTAAAACAACCCTGTTATATAGGGATAA720.1944526966808005No Hit
AGTTCTGGGACATTTTCAAGTTTATTAGGTTAAGAAAAACAGGGCAGCCTTG680.18364976908742267No Hit
ACTAAAGGGGATTTTTAGAACCTTTGCCTATATTAGGTTGTACTTATGTACA680.18364976908742267No Hit
GACATGGGGACTGAAACATGCTTTAAATCGTGTCTAAAATCTGATTTGAGCA680.18364976908742267No Hit
GCAGTTGGGAGAGACCAACCGAACCCCCTTCGACCTTGCCGAAGGGGAGTCC670.18094903718907823No Hit
TTTACTTGGGGGAGTCAGTCATCCAATTTTTCATGACAGAAAAACAACATTT650.17554757339238933No Hit
ACACCGGGGAGGTCAGCCATATCCAAAAGACCACAAGTCATTACTAAGTTGA650.17554757339238933No Hit
ACTAATAGGCTCTCTGTTGATATTTAAAGAAGTGAGGGCTGTGGGTATTCAT650.17554757339238933No Hit
GTGATTGGGGTTACTTTTCTAGCAGATTTTAACAAATAATCAAGGACATTTT640.1728468414940449No Hit
GATTTTGGGCTGCCACTCTTAACACTAAGTGAGAGTTGGGGAGAAACTGAAG630.17014610959570042No Hit
ATAAATGGGGGGAAGAAGGAAGGAAGAGTAAATATTAACAAACATGATTTTT630.17014610959570042No Hit
GAGAATGGGGCAGCCTTCCTCTTCTGGGACTGCTGTGAGGCAGAGGAATGAT630.17014610959570042No Hit
TGAATGGGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTC620.16744537769735598No Hit
ACGTTTGGGGTAACTAAAGTTACTGGCTTGGTCTTATTTGCACAGTTTTTGC590.15934318200232264No Hit
AGAACAGGGGAGAGGAGTTCCAGCTGTTCTGGCCACACTGGGCTGGTGGGGA590.15934318200232264No Hit
GTAGTGGGGACCTCCCACAAGGATTACCCCTTCCATGAAGAGTTTTGAAGCT580.15664245010397818No Hit
TACTTGGGACAGAAGAGAACTCCTAGAAGAGCTTTACTTAACTATGAAAATT580.15664245010397818No Hit
GCGTAAGGGCAGAAAAGTAGTGAACTTATTTTCAGTAGACATAGAAAACAAT580.15664245010397818No Hit
ACAGATTAAGGTGGATACAAACCCTGTATGATCAGAGAAAGCACTGGAACTG560.15124098630728927No Hit
ACTTTTGGGAGGATGGCAATTGCTCAGACTTGTCCTTTGCTTGTCTGGTTAT560.15124098630728927No Hit
TTCCAAGGGACAGTCCTTCAGCAGATTGATTCAGTGGCTAATGCTGATATCA550.14854025440894483No Hit
CAGTTAGGGGGCAGAGGACGATAAGCACTGCTTCCCGCAGGCATTTTAAAAA540.14583952251060037No Hit
GCTAGGGAAACTGGAACAGTTTACTACCATGAATCTATCCTATGTCTTCATT520.14043805871391146No Hit
ATAGCAGGGAACAGTCGTATGATTTCTAGCCCATGGGTTTAAAACTGTATCT510.13773732681556702No Hit
CTCTTTTTCTCAGTAAATCATATCATCTGAAATATTACAAATTTCAAATTTC510.13773732681556702No Hit
TACTAGTGGGAGCCCACCAGGGTCCAGGGAGATGTTCGTTCTCGCTTTAAGT500.13503659491722256No Hit
TAATGCGGGGCTTTTGAAGTAGTGTTCTGTTTAAACTGGCGCTCTGGTCTAT490.13233586301887812No Hit
TAAGCCGGGAGCCCTCACAGATGCCCAGCCTCATAGCTGAAGTTGCCTGAAT480.12963513112053368No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTCTTATA470.12693439922218921No Hit
TCAATGGGGACTGGTGGGTGCTCTGAAAACCCACAGGATCCCACCTCCAGGC470.12693439922218921No Hit
GGTAGTGGGATCAACGGGATATCAAAATGATTTTGAGGATAGTAATTTGAGG470.12693439922218921No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGA460.12423366732384475No Hit
ATTATAGGGCAGAGACTTGACTGCCAGTTGAGTAAAGGTGCAAGATATGAGG460.12423366732384475No Hit
CTTAATGGGAATTCTATATTTTCTGGCAAGTTTCTATACGAAGTATGATCCA460.12423366732384475No Hit
GTTATGGGGAGCCTTGGGAAGCATTTCCATCTATCGGGACACCCACCAGTCG450.12153293542550031No Hit
TTTGGAGGGAATCAGAGATGGCACAGATAATAAAAACAGGAATGGATATGGA450.12153293542550031No Hit
TTCATGGGGCAAGAAGTATAATATGCGTGTGGAAGACTACGAACCTTACCCG450.12153293542550031No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTC450.12153293542550031No Hit
GGCTATGGGGGGTGCGGAATCAAATAGGAAGCATGCAGAGACTCGGCAGCTT440.11883220352715586No Hit
GTTTTGGGAAAGACTACAGTCATACCTCAACTATCTACAGAGGACTGGTTCC430.1161314716288114No Hit
CTATCAGGGCAACTCCTTGAGGGTAAAAGGGAACTCCTCCTTTTTGAAAGCT430.1161314716288114No Hit
GTTAATGGGATACAAACTGATGTTGACTTTTGTGAGATATATCTTCTGTATC430.1161314716288114No Hit
TCAATTGGGGATTAAAATAATTTGAAGGCGATCTTTTAAAAAGAGATTAAAC420.11343073973046697No Hit
ATCACAGGGACTTTTCTGATATGAGGGCAGCACCTGCCTCTGAATGGGAAAT420.11343073973046697No Hit
GTGCTTGGGGTAAACCAAAAGATGGAAGACAAAAAGACAAACACACCCACAC410.1107300078321225No Hit
AAGCAAGGGGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAA410.1107300078321225No Hit
TCATCAGGGCATGGCCCACATGGCCTCCAAGGAGTAAGACCCCTGGACCACC410.1107300078321225No Hit
TGTGCCGGGATACCTACTACTGTGTCATGAACATCTTTGTTAGCAATAATGG400.10802927593377806No Hit
TATCCGGGGGCGCATGCTCCGTTGTCCCTTCAGACTTCACTTATAACACACA400.10802927593377806No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCC400.10802927593377806No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGCCGTCT400.10802927593377806No Hit
ACGCTGGGGACTTGTAATTGTGCCTCAGACTTTTTGAAAGTCTACCTTCTAA400.10802927593377806No Hit
CATTTCCCATTAAGTCTGAGAGGCAAGAGCTAGGTTAGGCAAGGAGGTGGTT390.10532854403543361No Hit
GTCGACGGGATGCACTTGGAGTCCATGAGCCAATGGGATATGCAAAGACGCT390.10532854403543361No Hit
GTTATAAGGATACCAATTAAACATTTTCATAGTTCTGCCTATTGTCCTTCCC390.10532854403543361No Hit
AACTTTGGGAGGCCGAGGTGGGCAGATCACTTGAGGTTGGGAGTTCGAGACC390.10532854403543361No Hit
GATGCCGGGTCTCTCTGATTGTATGTGGGAGACACCCATTGCTCAGGCCAAA380.10262781213708914No Hit
GTTAGAGGGCTGAAGCGAGGGATATAAATTACAAAAGAGATTTTAAATTAAT380.10262781213708914No Hit
CAAGGTGGGATCTCCCCTACCAGCAGATTCTGCGAAAGCACCAAATTTCTCA380.10262781213708914No Hit
CATTTTGGGGATTTGTATGATCTTATAAAATGAAATAAGCAGACTTAAAAAA380.10262781213708914No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)