FastQCFastQC Report
Sat 31 Dec 2022
SRR5844517.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844517.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105987
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT26992.5465387264475834No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC20731.955900251917688No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA3350.3160764999481069No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC3320.313245964127676No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2950.27833602234236277No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA2260.2132336984724542No Hit
GACTGGGGGAGAAAAGTATTTTTAGAAAAATAATCCTAGTGGCAAAATGGAA1760.16605810146527403No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1620.1528489343032636No Hit
GAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCG1530.1443573268419712No Hit
GAAGAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA1440.13586571938067876No Hit
CTAGCGGGGACCACAGAATCCATTTTTTTCCCAGTTCCACAAAACACTCTGT1370.12926113579967355No Hit
TTCTGGGAGTGGCAGGCAGAGGCCCAGGCTGCCGTCCCCTAGAGTCCCAGGT1250.11793899251795031No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT1230.11605196863766311No Hit
ACGCCTGGGCACCTCCTGGGCGCAAGCAATCCTCCTGCCTTAGCCTGCCAAA1210.1141649447573759No Hit
TGCCTGAAAAAGCGTCAAAGAAGCCAAAACAACATCCCAGTGCCAAGATTCT1190.11227792087708871No Hit
GTCCTTGGGAAGATGGAGGCGACTACGGCTGGTGTGGGCCGGCTAGAGGAAG1160.10944738505665788No Hit
TTCTAAGGGATAGTATATGATTTACTTTTATATTTTAATAGATAATTCTACT1140.10756036117637068No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCC1120.10567333729608347No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTCTTATA1080.10189928953550907No Hit
TCTGCAGGGAAGTTCATTCCAGGAATTTGTTTCACTGCACTTTCTAGGTTTC1070.10095577759536548No Hit
AATCACGGGAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATA1070.10095577759536548No Hit
TAGTGTGGGTGATTATATGCTTGTCTACATAGGAAAAGGAAAGAATCTTGAG1060.10001226565522187No Hit
TGGTAGGGGTGACAAACACAATCACAATACAAATAAAACGAATGATTTTTCT1060.10001226565522187No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)