FastQCFastQC Report
Sat 31 Dec 2022
SRR5844516.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844516.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105182
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT27002.5669791409176477No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC22192.1096765606282446No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC3600.3422638854556863No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2990.2842691715312506No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA2830.2690574432887756No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA2050.19490026810671027No Hit
GACTGGGGGAGAAAAGTATTTTTAGAAAAATAATCCTAGTGGCAAAATGGAA1940.18444220494000874No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1730.16447681162176037No Hit
GAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCG1680.15972314654598696No Hit
GAAGAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA1480.14070848624289328No Hit
TTCTGGGAGTGGCAGGCAGAGGCCCAGGCTGCCGTCCCCTAGAGTCCCAGGT1420.13500408815196516No Hit
CAAGCTGGGCACACTTATTCCCCTGTATGCAGTTTTGTGGACAGTGTTCAGG1230.11694016086402617No Hit
GCTATTGGGACTTGCGCATTTCCATAACTTTTGTTTTCTTAGGCCATAGTCT1190.11313722880340743No Hit
CTAGCGGGGACCACAGAATCCATTTTTTTCCCAGTTCCACAAAACACTCTGT1170.11123576277309805No Hit
CTACGCGGGAGATTCAGAAGTTGTCACCTTTGAAATTTTGTCTGATGACTAC1130.10743283071247933No Hit
TCTGTCGGGCAGCCATGAAACAGTAATTGAAAATGTTGACTTTATCAAAATT1110.10553136468216995No Hit
ACAGCAGGGGTGAAAGTAACACTTACCTTCTGTGCAATTGTCCTTTGTACAA1070.1017284326215512No Hit
GAAGGGGGGATTTGGCATTTGACTTCGTGGATAAAATCTTACTCGATTTCTC1070.1017284326215512No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT1060.10077769960639654No Hit
GGCATTGGGGAAAAATACTACTTCTTTAGCTTGGGCTACATGTGTCAGGGTT1060.10077769960639654No Hit
TGCCTGAAAAAGCGTCAAAGAAGCCAAAACAACATCCCAGTGCCAAGATTCT1060.10077769960639654No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)