FastQCFastQC Report
Sat 31 Dec 2022
SRR5844515.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844515.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105228
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT25982.468924620823355No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC19941.8949329075911354No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC3360.31930664842057244No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA3090.29364807845820506No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2980.28319458699205535No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA2360.2242749078192116No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1840.17485840270650396No Hit
GACTGGGGGAGAAAAGTATTTTTAGAAAAATAATCCTAGTGGCAAAATGGAA1690.16060364161629986No Hit
GAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCG1610.15300110236819098No Hit
GAAGAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA1410.13399475424791882No Hit
CTAGCGGGGACCACAGAATCCATTTTTTTCCCAGTTCCACAAAACACTCTGT1220.11593872353366025No Hit
GCTATTGGGACTTGCGCATTTCCATAACTTTTGTTTTCTTAGGCCATAGTCT1170.1111871365035922No Hit
CAAGCTGGGCACACTTATTCCCCTGTATGCAGTTTTGTGGACAGTGTTCAGG1140.10833618428555138No Hit
TGTAATGGGGCAGGCAAAAACCACAATTACTTTTGTTCCAACCTAGTACATA1090.10358459725548334No Hit
TCTGTCGGGCAGCCATGAAACAGTAATTGAAAATGTTGACTTTATCAAAATT1090.10358459725548334No Hit
CACACTGGGATGGACTTAAGGTTTCATCCCGCTTTATGAGTGCTTCTGAATC1080.10263427984946973No Hit
AGGTTAGGGAGAAAAGTTCAGAACACAATGCCACGCTTCTTCGACAATGAAG1080.10263427984946973No Hit
TTCTAAGGGATAGTATATGATTTACTTTTATATTTTAATAGATAATTCTACT1060.1007336450374425No Hit
TCTGGGAGTTCCCACCCATGAGTGAGAACATGCTCGCACCGCCGCTTCTAAA1060.1007336450374425No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)