FastQCFastQC Report
Sat 31 Dec 2022
SRR5844510.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844510.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36880
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGC1190.32266811279826463No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGCCGTCT1000.27114967462039047No Hit
CTTAAGGGGATCTTTACAATCCCTTTTTACCACCAATAAAAGGATTTTTCTT530.14370932754880694No Hit
CTGCAAAGGGAGAACAATATAAAACTGAATGAAGGCTATGAAAGAAGTCACA520.14099783080260303No Hit
ACAAACGGGAGAACTAATAAATAAATTCAGTATGATTAACAGGATGAAAGAA510.13828633405639912No Hit
TTACACGGGACAATTTTATCTTCTGGCAAAGGGACATCATTCCTGTATTATA510.13828633405639912No Hit
TAATGTGGGATGAAGCCTCTTACGAAAAAATGCTTAAGCTGCGACGAGATCT510.13828633405639912No Hit
CTGCATGGGGAGGTCAGATGACATAATGAATATGTTCTAACTTCTGAAACTT500.13557483731019523No Hit
ATTGCTGGCAAACTCCTGGCCTCAAGTGATCCACCCACCTTGGCCTCCCAAA500.13557483731019523No Hit
TCGTCAAGGGCTGCCCTCAAGCGGTTCATTTTCTTGGGAAGGAGGAGAGCAG490.13286334056399132No Hit
CGCTCTGGGATGCACTTGCAGGCTTTGTTTTGTGTTTAGAAGGTTGTTTGTC490.13286334056399132No Hit
GATTCAGGGGACTAAATCTGAGTCAACTTATGGATTTACCAGTTTGTTTAGA480.1301518438177874No Hit
TCTACAGTGGAACGAGGGAAAGCCCTCCACCAAAAGCAAGCCCTTAGAAAAA470.12744034707158353No Hit
TCATAGGGAAGAAAGATGAGTCCACTATAAAATGTATTGCTAAGATTTCATA470.12744034707158353No Hit
TTAGCGGGGATAAGCACCAGTGAAGAAGTTCTAACTTTTAGCATGCTGCACA470.12744034707158353No Hit
TTGACGGGGGGAACACACATTCCTTTTGACAGGGTCTGTCACTGGGTGGGTG460.12472885032537961No Hit
CAGTCTGGGATCAAAGAAGAAATCTGATTGATCCTGATTCTCTCATCTCTGG460.12472885032537961No Hit
CGGCGCGGGAGTTTGAAACAGAAGCTGGAGGCAAAAGAAATCTCTGGAGTTG460.12472885032537961No Hit
TATCTCGGGAGAGAGGGCTCTAGCTCATGTTACCTGGTTCTCAATTTCTGCT460.12472885032537961No Hit
AAGAACGGGCCACCGGAGCCGCCTCGGTCAGGGCCACCACTGCCCAACGGCC460.12472885032537961No Hit
TCGAACGGGGATTTTAATCTTACCCTTCTTCTAAAACAGGGGTTCTTAAGCC450.1220173535791757No Hit
TAAGAGGGAAACTAATATATGTGAGAGTACTTAGTTGAAACAAAAAGGAATT450.1220173535791757No Hit
TCCAAGGGGGGATCTGAAAGCGGAGGGCGGGGAGTTGCCCCGCCGCGGGCCC440.1193058568329718No Hit
TTTGCAGGGAGTCAGACTCTCTCATTCTACATTGCTGGAGATAATGATTGAG440.1193058568329718No Hit
CGAACAGGGATTAGTTGACATGCATGGAGGACTACTTGGATGCCAAAAGCTT440.1193058568329718No Hit
GAATAAGGGCAGGTCACGGAGCTGCTGGACGTCTCCATGGAGCTGGGCTGTT440.1193058568329718No Hit
GTGCCGGGCAGCAAAAAGACTTCTACTAAATAATGATCAGAAGATTTTTTTT440.1193058568329718No Hit
TGCTTAGGGAAACTAAGTCAACCCTTTTAAAGACTTCTTGAAATTTTGTACC440.1193058568329718No Hit
ATCGTAGGGTCCGCAACTACCCTGCTGCTGCTGCTGCTGCTGCCCACAGAAA440.1193058568329718No Hit
GGTTCAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG430.11659436008676789No Hit
GATCATGGGAGCCATAATAATGAATACCAAAATCCACCTTTTATTAGAGATT430.11659436008676789No Hit
GCGTATGGGCTGAAATATGTATAGTTAATCGTTAGAGCATGGAGACAGTTGT430.11659436008676789No Hit
TGTAGTGGGAGCTCACTGCAGCCTTGAAATCCTGGACCCAAGTGATCCTACC430.11659436008676789No Hit
CTTGCCGGGAGAACAGTGCCTGGCACATGGTAGGTGCTCAATATGTGTTTAC430.11659436008676789No Hit
GCTGAGGGCACAGCCTAATTATTAGCATCATCCCTCTACTATTTTTTAACCA430.11659436008676789No Hit
AGGCATGGGACCTACCTGCTGATTGTGCTACTTTCCTACTCAAACATCTTGT430.11659436008676789No Hit
CTTCGGGGTATGTTTCTTTAACATGGCTTAAATTTTGAACTTAATGTATCAA430.11659436008676789No Hit
ACATTTGGGCAGTTTTCTTAAAGCACACATGCAAAAATGAAATGGTCCTAAA420.113882863340564No Hit
ACTCATGGGGTGGTTTGCCAGCAGTCAGCCACACAACGACTGGAACATGCAT420.113882863340564No Hit
ACCAATGGGGTGAATGGGAGGGTGCAAGCCCCAAGCCTTGGTGGCTTCCATG420.113882863340564No Hit
AGCAGCACTTTCCCTAGCAGGTTTGCCTCTATAACTGCAAAAGAAGTCTCAG420.113882863340564No Hit
GATTTTGGGGGGGAGGTGCTTGTGTGCCTGGTGCGGGAGCTACGGGGCCCAG420.113882863340564No Hit
ATAAAAGGGGGTGCCAAGATGCTGTAATAGAATAAAATTGCTTACATATGAT410.11117136659436008No Hit
ATGCATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA410.11117136659436008No Hit
CGTTGAGGGGGGCGGCGGCGGCTGCGGTGGCCAAGCAGGCAGATACTGCCTG410.11117136659436008No Hit
CGCGATGGGAGGGAGTCTCGTCCGCCCAAGCGGGCGCATCTGGGTCTGGCCC410.11117136659436008No Hit
TGAACTGGGAGACACTGTTCAAAGAACTTCATGTTAACTCATTTAATCCTCC410.11117136659436008No Hit
GTACTTGGGACTGAAAGTCGAGAAGGCCTTGCACAGCAAGTTCAACAGAGTT410.11117136659436008No Hit
TTGCAAGGGGACCGCTTTCGCTTTCCCTTCGCGGTGCCCACTCCACTCCTTG410.11117136659436008No Hit
ATTAACGGCGACGCTCTTCCGATCTAATTGTGGGATTGGGGGCTCACCTTGA410.11117136659436008No Hit
TAAGACGGGTAGACAATGTGGAATTACATAACAGGCATTGCACTAAAAGTGA410.11117136659436008No Hit
GTAGAGGGGTTCAAAAAGCATCAGTGATTTCACAATTCTTCCACTCAAGCTT410.11117136659436008No Hit
TACATAGGGGAAGAAAGCCTGGCAACAGCCACCATTTGTCTTGTGGACCTGA410.11117136659436008No Hit
CTTGGTGGGAGCCTGAAGGACATTTTCAGCATATTTATACATGAGAAACAGC410.11117136659436008No Hit
GTAGACGGGCTGGATGAACTTGTACAGTCATTGAAGGAATACGCCGATTATG400.10845986984815618No Hit
TTTCATGGGGTGTCTGTTCATGTCCTTTGCTCATTTTTCAATGGGGTTGTTT400.10845986984815618No Hit
ATAAGTGGGGGGTGTATGGGACACTGTGAGTGATGTACTCTTGGAAAAGGAG400.10845986984815618No Hit
ACCTCAGGGGTATTATGTATAACTTATTAAATGTATTATGTATAACTAATAA400.10845986984815618No Hit
ACGTCCGGGAAGGTGAAGACAGTCTGCCACTTTAGTTGTTCCCTTGATAGTA400.10845986984815618No Hit
TCTTTTGGGGAGAAATTCAGCATTAAAGAACACTGAAGCGGTAAGGTCACTG400.10845986984815618No Hit
CGTAGGGGCAGTGTGGTTTCAACCCTACTTCTGCATCTTAAAGACACTGTAT400.10845986984815618No Hit
GTCGAAGGGTGGATTTAACCTGAGTTGTACTAAATAATTTTGAGCAAGTAAG390.10574837310195229No Hit
AGTCACGGGTGAAAGGTTACTAGCAGGTTATTCAAAGGTACTTTTTTTGAAG390.10574837310195229No Hit
CTGAGTGGGGTGTATACAAGTGAGATTAACTAACAACAGCTAAAGTGAAAGA390.10574837310195229No Hit
CGACGCGGGTATTTCTGCAGCTACCTCAATCATGTATTGCCTGTCTCTATTT390.10574837310195229No Hit
GGTTATGGGTGGTTTGTACATCATTATTTCTCCTGAATTGCAATTAAACTCC390.10574837310195229No Hit
CAAACTGGGGTACCTTACCTCTGATGTGGAGTGTCTTACATGTGGCTTTAAT390.10574837310195229No Hit
GGGTCTGGGTGGCAGGAAGCTTGTTGCGGCATTGATCTAACCTTTTTCCCCC390.10574837310195229No Hit
TCAAACGGGAGATGAGGAAGGTGAGGCATAAATGATGGGGTGACTTACTTGA390.10574837310195229No Hit
CACTTAGGGGGATGTGGAAAACCTTTCCTAGCTTAGAGCATTTGTATCTACA390.10574837310195229No Hit
ATTCCAGGGAATGAAACATATTCCATGTATCATAAAGCCTACTCTCGATGGC390.10574837310195229No Hit
ACAGGTGGGAACACGAGAACAGTATGGGGAAACTGCCCCCATGATTCAGTTA390.10574837310195229No Hit
CAACAAGGGTCTCCACAAGGGGGTGGGCAGCATCGCCACACAGTACATGACC390.10574837310195229No Hit
GATTTAGGGAGTAAAACCTGGTGCAGTAGGGGCAGGAGAGTTTGTGAGCCCC390.10574837310195229No Hit
GTGAAAAGGGGTATTTTAAATAACAATGTCTCTTGAAGGACACTGTGTATAG390.10574837310195229No Hit
TGTAACGGGATACTTCTGCTGAGTTTAATATTCATCTAGAGTCAGTTGTGTT390.10574837310195229No Hit
GAGCGGGGGGGAACGGGAAGAGCCACTGACCCTGAGACCAATTTCCAGGCAG390.10574837310195229No Hit
TCTACGGGGAGCGCAGTCTCGGCCACATCTCGCTACATCGAGGAGCAGCAGG380.10303687635574837No Hit
AACAAGGGCTGAAAACCACTCACAAAACAATACCTTCTTTTATATGTGAAAA380.10303687635574837No Hit
TCTACGGGGCTCTAGCGGCTAGTTGCTGTGGCTCTGGACACTAACGGGCCTG380.10303687635574837No Hit
TGTGCTGGGAGATATGGTGATGTGATTAAAGCAGCACACATTCCTTTTGGTG380.10303687635574837No Hit
CGAACAGGGAAGTTGTTGTTACCGTGTGCCAATGTGTCCCATGTGGGTTGTG380.10303687635574837No Hit
AATGCTGGGTGCCTTCAAAGATCAGAAACATGATTTGTTCATTTCTGAGTCA380.10303687635574837No Hit
GATCAAGGGATCAGTTATCAACACACAGAATTGTTCAAATAGTAATTAACAG380.10303687635574837No Hit
TTTTACGGGAGGCTTCAATTCAACCTTGAAGCCATTTGGGCCTGGGCTTTTT380.10303687635574837No Hit
CGATAAGGGAGGACAGTTCACAGCAAATTGTTTACTTACATGGCATAATTGA380.10303687635574837No Hit
ACACCCGGGTTTACAGGTGAAGAGTGCGACATTGACATAAATGAATGTGACA380.10303687635574837No Hit
CAGCTAGGGGTGTAAATTCTCAAACTGGAATTATCTTTACCTTACTTGGAGC370.10032537960954448No Hit
AATCGCAGGGTAATATCACTGTGTTGTAATTATGAAGGCCTAGTTAGTAAGG370.10032537960954448No Hit
CGAAAAGGGAAGAGGATGTTCTTATTGGCCTACTCAATATGGAACTACAAAG370.10032537960954448No Hit
TCCAGCACTGATTTTGTACATAAACATTAGGCAGGTTGCTTAACCTTTTTAT370.10032537960954448No Hit
ATCGGTGGGACTCTTAATCATGAAACTTGATTTTCTCAATTGGCCAGTCTTC370.10032537960954448No Hit
CAACTTGGGCACTATAGATAAGTGTTTTCATATACCAGTGAGTATTTTTTTG370.10032537960954448No Hit
CTGCATGGCGGGTTTGATTGTCTGTCAGCCATTCAATAGATTGAGAGTGACA370.10032537960954448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)