FastQCFastQC Report
Sat 31 Dec 2022
SRR5844509.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844509.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35513
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGC1080.3041139864275054No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGCCGTCT1050.29566637569340803No Hit
TCGTCAAGGGCTGCCCTCAAGCGGTTCATTTTCTTGGGAAGGAGGAGAGCAG580.1633204741925492No Hit
TACTTAGGGCTACTAATGCTGTTATTTGTTTATTTAGGAGTTAGTTTCCACT540.1520569932137527No Hit
GGCCCAGGGAATGTCTCATTTATAGAACATTATTTTACAATGAGGTTTTACA470.13234590150085884No Hit
GACCATGGGCATAGCAAATTACATTTAATATTTTCTGTTTTTAATAAATGTA460.12953003125615972No Hit
GATGATGGGCCAGCATGAGGAAAACATCCAGAAAGAAATCACTAATTGAAGG450.12671416101146057No Hit
GATTCAGGGGACTAAATCTGAGTCAACTTATGGATTTACCAGTTTGTTTAGA450.12671416101146057No Hit
GCGTATGGGCTGAAATATGTATAGTTAATCGTTAGAGCATGGAGACAGTTGT450.12671416101146057No Hit
AGCCGCGGGACTTGGCATTCAACAATATAAGACCATTACATAATTTTTACTT450.12671416101146057No Hit
GCAACTGGGTCTAGCGGCGGGCGCGAGGTTGTCTGCTAAACCTGCTCCTCCA450.12671416101146057No Hit
CAACTTGGGCACTATAGATAAGTGTTTTCATATACCAGTGAGTATTTTTTTG450.12671416101146057No Hit
ATTCGAGGGGAGACGACGACGAAGAAGCAGGAGTGGCACAAGATCTCCTAAA450.12671416101146057No Hit
CTGAGTGGGGTGTATACAAGTGAGATTAACTAACAACAGCTAAAGTGAAAGA440.12389829076676147No Hit
CACATGGAGGGCACTCTGGAGGAATTCACTGTTAATTTTTAATGGTTTCTGT440.12389829076676147No Hit
GTACAGGGATGAGCTGCCGATGTGGTGCTTAGTGATTGCGGTTTCGGTCGCT440.12389829076676147No Hit
TCCAACCGGGGAAGGAAACAAACAAGAAATACAAGAAATAAATGAGACAAAA430.12108242052206233No Hit
AGTAAAGGGAGGGCCGTGCTCTCCGTGGAAATCTATGTTGGAGACGACAGGC430.12108242052206233No Hit
ACAGGTGGGAACACGAGAACAGTATGGGGAAACTGCCCCCATGATTCAGTTA430.12108242052206233No Hit
CGATAAGGGAGGACAGTTCACAGCAAATTGTTTACTTACATGGCATAATTGA430.12108242052206233No Hit
TACTCTGGGAGCAAAGTGCCCCTCCCAAGTTCTTTTCTAGTATACTGTAGTT420.11826655027736323No Hit
TGCAATGGGATAGGCTTCATACCTACTGTGTTAGTTTTAGTTTCCTAGGCCT420.11826655027736323No Hit
TTTGCAGGGAGTCAGACTCTCTCATTCTACATTGCTGGAGATAATGATTGAG420.11826655027736323No Hit
GCAATGGGGATTTCTGAGATATATTCCATTGTGTGAATATACCATGATTTGT420.11826655027736323No Hit
AGGAAAGGGATAGCAAACAGTTTTCCAAAATGTTTTAAGCAATTTATACTTC420.11826655027736323No Hit
TCAAGTGGGACTTCAGGCATTCAGCAGATTTCAGAAAGAATTACCTTATTTT420.11826655027736323No Hit
TAATGTGGGATGAAGCCTCTTACGAAAAAATGCTTAAGCTGCGACGAGATCT410.1154506800326641No Hit
AATGCTGGGAATAATTTAAGTAATGAAAACACTACATATCAGAGTATTTGAA410.1154506800326641No Hit
ACTCATGGGGTGGTTTGCCAGCAGTCAGCCACACAACGACTGGAACATGCAT410.1154506800326641No Hit
GCTGAGGGCACAGCCTAATTATTAGCATCATCCCTCTACTATTTTTTAACCA410.1154506800326641No Hit
GACATAGGGCAGCCAATTTAGGAAGTTAAAAAGAACAACAAAATAAACTCAA410.1154506800326641No Hit
GTGGTTGGGAAAGAGACAACCCACAGAATGGGAGGAAATATTTGCAAACTAC410.1154506800326641No Hit
CGAACAGGGATTAGTTGACATGCATGGAGGACTACTTGGATGCCAAAAGCTT410.1154506800326641No Hit
ATCGTAGGGTCCGCAACTACCCTGCTGCTGCTGCTGCTGCTGCCCACAGAAA410.1154506800326641No Hit
GATCATGGGAGCCATAATAATGAATACCAAAATCCACCTTTTATTAGAGATT400.11263480978796497No Hit
CTTCACGGGATCTAATATGAGGAACTGAGTAAAAAAGAACCACTTTTTAAAT400.11263480978796497No Hit
TCATAGGGAAGAAAGATGAGTCCACTATAAAATGTATTGCTAAGATTTCATA400.11263480978796497No Hit
TTAGCTGGCCATAAAGAAGAGTGAATAGCAAATAGATGGTTACAGTCCAGAA400.11263480978796497No Hit
GATTTTGGGGGGGAGGTGCTTGTGTGCCTGGTGCGGGAGCTACGGGGCCCAG400.11263480978796497No Hit
TTGCCGGGGAATCCTAAAATGGTTATTTTCAGTGAATATTGGACTGATATCC400.11263480978796497No Hit
AATGCTGGGAAGATGGTCTTCAGATAATTTATACTTTTATAGAGCATAAAAT400.11263480978796497No Hit
GGTTCAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG390.10981893954326585No Hit
ACAAACGGGAGAACTAATAAATAAATTCAGTATGATTAACAGGATGAAAGAA390.10981893954326585No Hit
TATAAAGGGCGACATGGTTCCTGCTGTCATGAGCCTTCCAAAGGAATAGCGA390.10981893954326585No Hit
CAATGAGGGGATAGTGAAAATCATACGCAATAAAATATTGATGTTTGAAGGC390.10981893954326585No Hit
TGAACTGGGAGACACTGTTCAAAGAACTTCATGTTAACTCATTTAATCCTCC390.10981893954326585No Hit
GTAGAGGGGTTCAAAAAGCATCAGTGATTTCACAATTCTTCCACTCAAGCTT390.10981893954326585No Hit
ACGCAAGGGGCGACTGAGCCGGGTGGATGGTACTGCTGCATCCGGGTGTCTG380.10700306929856672No Hit
TGTAGTGGGAGCTCACTGCAGCCTTGAAATCCTGGACCCAAGTGATCCTACC380.10700306929856672No Hit
GCAGGTTGGGGTAACAAGAAACTGGTCAAATTTATTAAAAAGCTCTTATTTT380.10700306929856672No Hit
GGGTCTGGGTGGCAGGAAGCTTGTTGCGGCATTGATCTAACCTTTTTCCCCC380.10700306929856672No Hit
CACACCGGGGGATATGAACTTCTTATTAGATATATGGCTTGCAAATAATTTC380.10700306929856672No Hit
TTGCTAGGGAGCTTGGAAATGACTGAGCCAGGATTGAAATGCAAGTCACTGT380.10700306929856672No Hit
CTATTAGGGTCTTACAGCGGGATAACTAAAATTGTAATGGAAGACTGCAATT380.10700306929856672No Hit
GAATAAGGGCAGGTCACGGAGCTGCTGGACGTCTCCATGGAGCTGGGCTGTT380.10700306929856672No Hit
GACCTCGGGAGCTCTGCTTCTTGTCAGTTGGGCAATGTGGGACAAGTTAATC380.10700306929856672No Hit
TCGAACGGGGATTTTAATCTTACCCTTCTTCTAAAACAGGGGTTCTTAAGCC370.1041871990538676No Hit
CGACGCGGGTATTTCTGCAGCTACCTCAATCATGTATTGCCTGTCTCTATTT370.1041871990538676No Hit
ATGTCAGGGTAGTTACTCTTGGCTGCAGAAATAGGTTTGGGAAGCTATGAGA370.1041871990538676No Hit
ACCTGGGACCTAAATCCCAGAGCTGAAATCAGCAATTTAATTGTCTTCCTTT370.1041871990538676No Hit
AGGCATGGGACCTACCTGCTGATTGTGCTACTTTCCTACTCAAACATCTTGT370.1041871990538676No Hit
AGGCTGGGGAATTGAGGTGAAATAGAAAATGAATGTCAATAGTAATCATTTT370.1041871990538676No Hit
TTCTAAGGGACAGTGCTTCCTCTGATATCTTTCCTTACATCATTATACACTG370.1041871990538676No Hit
GCATCCGGGTAGCAAGGGCAATATAGCCTTCTCCTTAAATCTTGAGGAAAGC370.1041871990538676No Hit
CTTGGTGGGAGCCTGAAGGACATTTTCAGCATATTTATACATGAGAAACAGC370.1041871990538676No Hit
TCCAAGGGGGGATCTGAAAGCGGAGGGCGGGGAGTTGCCCCGCCGCGGGCCC360.10137132880916847No Hit
CAGCTAGGGGTGTAAATTCTCAAACTGGAATTATCTTTACCTTACTTGGAGC360.10137132880916847No Hit
AGGCATGGGATAGATAGCAACACAATAATAGGCGGTCACTCCAATTCTCCAG360.10137132880916847No Hit
ACTTCAGGGCGATCTGCAATGGGTAGAAAAGCAGGGGTTGCAATCCTAGTTG360.10137132880916847No Hit
GCTTCAGGGATGCCCTCTCTCACCACTCCTATTCAACATAGTGTTGGAAGTT360.10137132880916847No Hit
CGTAATGGGAGAAAAGAAATATAAATAAATTATTAACATATCTTTGCCTAGA360.10137132880916847No Hit
ACGGCGGGGATCTACGCTAGGGAATGCCCCAACTAAATACTACCGTATGGCC360.10137132880916847No Hit
CAAGTCGGGTTATTTCTCCTCATTTCTTCCTTATGTTTAAACACGCCCCCAT360.10137132880916847No Hit
GAATAGGGATAACCTGACATGTCCACCCTACTGCTATGTCCAGTCTCCATTG360.10137132880916847No Hit
GCCTGGGCGACAAGAGTGAGACTCGATCTCAAAAAAAATTATTTGTAATTAA360.10137132880916847No Hit
CTGCATGGCGGGTTTGATTGTCTGTCAGCCATTCAATAGATTGAGAGTGACA360.10137132880916847No Hit
CTTGACGGGGGAAAAGGCATTGCTTTTACTATCATCCTTGTAAAATCTAGTT360.10137132880916847No Hit
CGACTGGGTCAACCAGCACCTAAAAAGACTCTTTTCAAAAAATCACAGAAAC360.10137132880916847No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)