FastQCFastQC Report
Sat 31 Dec 2022
SRR5844508.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844508.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19297
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1860.9638803959164636No Hit
TAAGCTGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT1830.9483339379178111No Hit
GACTCAGGGGGGAGCCGCCGGGAGTGGACGCCGCCGAGGCCCGGAGTCGCGC1830.9483339379178111No Hit
GGTCAGGGGGCAGAGCTGATTGGGAGGCTGGAGAGCAAAGCACTGTGGTTCG1760.9120588692542884No Hit
GAACAAGGGCCGCACTAAGGCCGGCTCTTGTGCCGGAAGGAGGAAGGCGTGG1740.9016945639218531No Hit
TTAGCGAGGGGAGGAAGGGAGGCGGCGGCGCGAGGAGGAGGGAGCGGCCGTG1660.8602373425921128No Hit
TAAGAGGGGAGGCGGAGAGAGGCGGTAAGCCGCGAGGAGGAAAGGGACTCAC1570.8135979685961547No Hit
CGTTAAGGGCCCAAATCGATGGAGCCACATTAATAATACTTTTCATGCCTCA1530.7928693579312847No Hit
GACACAGGGTGTCTGTGTGGAGCACGTAGTGCACAGAATACGTGAGAGTTGC1520.7876872052650671No Hit
AATCATGGGAGTCAGATGCTGTGGTACTTGGCATTTAACAACTAGAATTCAG1470.7617764419339794No Hit
GAATATGGGGGCCTGTTTGGATTGGAAGAGGAGATCTTACACAGAGGAACTA1460.7565942892677618No Hit
GGCGATGGGAGATTTAGAAGAAAAGATCCTGTTTCCATTTGAAAGGAACTGT1460.7565942892677618No Hit
TTCTAAGGGGGACCTCCTCCCCCAGGCTTTAAGTCTGAGTTCACAGGACCAC1450.7514121366015443No Hit
CTTTGTGGGAGTTGATTCAGCGGTTGCGAAGAATGGAACGAAGCTTAAGTAG1450.7514121366015443No Hit
TGGCACGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1390.720319220604239No Hit
TCTAGCGGGATTTGTTGGTGGCATTAAAGAAGACACTGAAGAACATCACCTA1380.7151370679380215No Hit
TACAATGGGCACACTCACTACCTTGGGTTTGAAAATGGCTCAACCACCATCA1370.7099549152718039No Hit
GTAGAAGGGACAAGCTTTCCAGAAGCAATTTTATAACAGATTTCATTTTAAC1360.7047727626055864No Hit
TCTATGGGGAGGTGCAGGGGCTGCCCAGGACAAAGCGGGGGCAGGGGAAAGA1340.6944084572731513No Hit
ATCATCGGGGGGCTTCAGAAGTATCTGCACAATTAGAAAAGTCCTCAGAAGC1340.6944084572731513No Hit
TTTCTCGGGCCCAGACTATAATAATCCTGACTATGTAGACGGAAGTTTCTAT1330.6892263046069338No Hit
ATCGTTGGGAGATTTGCTAAAGTGTTCATGCCAAGTTTATAACTGACAGCAG1330.6892263046069338No Hit
GCTTTAGGGAGAACTGGATCTGTCGGAGCTGGAAGGCCTGGGTCTGTCAGAT1320.6840441519407161No Hit
TAACGTGGGGCACCTCCCGCATGGCCCCTCCGGCCAGCCGGGCCCCTCAGAT1310.6788619992744986No Hit
ATGCACGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1280.663315541275846No Hit
ACAATCGGGGCATTTTATAATAAAATGACAAACCAATTTTTTAAGTGAGCAA1270.6581333886096284No Hit
CACCGGGGATGTTTGTAGGGGGCTGTGTGGCTTTTATCCTGGATAACACCAT1260.6529512359434109No Hit
GTTTACGGGGTAGTACCACGAGGGACGCCAACTCCCAGAGGAGTCCTGTCCA1260.6529512359434109No Hit
TCACGCGGGCTCATTCAAATTTAAAGATATCTTTCTCCTCTTGATCAATCTA1220.6322226252785408No Hit
AGTCCAGGGGTCTTACCTCGTTGTGGCGTCAGAACCGCCGTCGCTCGCTCCC1210.6270404726123231No Hit
CAACAGAGGGGATCTGCAAATGGGGCTCACTCGCTCTGCCATGGTGCCATGA1210.6270404726123231No Hit
CATGATGGGATATCAGTATATAATGTTTTGAGACTATTATTACTCAATGTTA1210.6270404726123231No Hit
CTGCATGGGTGGCAACGATAACCTTAGAACAACTCATTTCACAGACCACCTG1200.6218583199461056No Hit
AGCCTGGGGGAGACCTTCGCATCGAGGGACGTGAGGGACATTTGTCCTAGAG1180.6114940146136705No Hit
AGTACTGGGAAGTAGAGGTTATTCTTCAGCATAGTGCTGCTGACATTGCCAG1160.6011297092812354No Hit
ACTCACGGGAGAGCTGCCACCCTCACCTGTCCCCAAGCCACCCTGTGTCCCA1130.5855832512825828No Hit
CCATGTGGGGAGCGCAGGGGTCACTGCACGCTGCAGGGCTCTTGCTGCCATG1120.5804010986163652No Hit
CTTACGGGGACTTCAACATGTGATTCAGAAGAATCACCTGGATGATGAGACC1110.5752189459501478No Hit
GTAGACGGGACATGTAAGCATGAAGGCTGGCTCGTTGGTGAGCCATTGCTTT1110.5752189459501478No Hit
TAAGTTGGGCCACTACGCCTGGCCCTGCTCTGGTTTTTATTCCCCTTTCTTT1100.5700367932839301No Hit
GCAAGCGGGGAAGAGTTTGAGGAAGAATTTGAGGAAGAAGAAGGTGAGTTAG1100.5700367932839301No Hit
AAAATGGGGATAACCATGCACACTACTATAACCACCCTAACCCTGACTTCCC1100.5700367932839301No Hit
GTGTTGGGGAGGGAGAAGATATTTAAGTGTCCAGAATGTGGGAAAACCTTCC1100.5700367932839301No Hit
TAAGCTGGGGGCTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTT1100.5700367932839301No Hit
GCGCTTGGGCTGATGGTTAATTTTAGACATCTACTTGAGTGGATTAAGAGAC1070.5544903352852775No Hit
CAGTCTGGGCAACAGACATCGCTATTGCGGTTCCGAGGCAGTGGGAAGAGAT1060.54930818261906No Hit
TCTCTAGGGACCGAGGAGTTAAAGGATTTGTCCGAGACCTTCAAGGTAACCC1050.5441260299528424No Hit
TTAGACGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1050.5441260299528424No Hit
TCTCAAGGGACTCAGGCTTGAGGCACCCGAATAAACAAAACTTAGAATTTAA1020.5285795719541898No Hit
GCTAAAGGGACTAATATATTGACTATATTTAATAATAAATTGTCAGATAAAA1020.5285795719541898No Hit
AGTGAAGGGATCTTTGATGAGGGCAGAGCTCACGTTGCATTGAAGACGAAAC1020.5285795719541898No Hit
TACCTGGGGCTGAGAGAGGGAGGTTGTGAATGCAGGTTTTATCAGAACTATG1010.5233974192879722No Hit
AATATAGGGATGTGAAATTATGCAATTTGACTTGATTCCTGAACTTCGTGCT1000.5182152666217547No Hit
AGCTTCGGGACCTATTGATGGGAACCCCCTAACCACAGCTGCAGGGCCAACA990.5130331139555372No Hit
AGTTGAGGGGGCTGCCCCCCAAAATATGCCAAGACCTTGTACCTGCTGTACG990.5130331139555372No Hit
GTCAATGGGACTGGGACATGAGTGAGCAGGAGGTACTAAATGCAATAGAGCA960.4974866559568845No Hit
TCACACGGGAAACCTGAAGGAGTTTGAAGGATTGTTGGCTCAGATGCGAAAG960.4974866559568845No Hit
ATGGTTGGGCCTTAATAATATAGTTTCTACAGAGCTTTCTTACTTATTTTTC960.4974866559568845No Hit
TCATCTGGGAGCCTACACAATTGTAGGTAGTGGCCTTGTCTTATTTACTAAA950.49230450329066694No Hit
ATGCCTGGGGCTGTTAAAGTAGATGACATCATGTGTTAGCCTGTTCCTAATC940.4871223506244494No Hit
GCACCTGGGATATAATGTCATGTAAAACTTTGCTTAAGATGGTCTGGTTTTT930.4819401979582318No Hit
TGCTGGAGGGACTCACCCTGCAGCTCTGACCTTTTTTCCCAAAGGGACTTAA920.47675804529201427No Hit
TGCAAAGGGTGCAAAGGAACAGGAGTGAGTCGTGACTGACAGGGGTGGTTGA910.47157589262579674No Hit
GGTTATGGGTACTAAAACTTTATAAAAATGTAAGTAATTATGTTGCCAATTT900.4663937399595792No Hit
ATCTATTGGGGATGATAGCACGAGTTTGTTTTTCAATTTTGCAAGATAATTT900.4663937399595792No Hit
TGCATAGGGGCAGGCGCAAGCCGGCAAGATGGCGGCGGCTGGGGCTGGCCGT840.43530082396227393No Hit
GGATGGGGGTCTGCGATAGGGATGTGAATTTTAAATTAGAATAACCGTCTTA840.43530082396227393No Hit
ATTTCAGGGATCTATCTAGATTTCTGAACCAAGATATATTTATAGTTCACTT830.4301186712960564No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGC810.41975436596362126No Hit
TCACAGGGATAAATTGAAAGGGCAGGTGGACAGAAAGAAGAGACTGACTCCT810.41975436596362126No Hit
GTATAGGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGC810.41975436596362126No Hit
GTGCTAGGGATCTCACATATTTTCCAGACACACTTATATCATTAGTCCATAG790.4093900606311862No Hit
GAAAAAGGGAGAGATTAATACAACTACTTAAAAAATATAGTCAATAGGTTAC780.40420790796496864No Hit
CAGTAAGGGCATGATGACCTAGGAGAGTTTCCATATGGATTGTTTGAACTTG760.39384360263253354No Hit
CTTGTTGGGGTGATTGATGTATCTAATGGGAAAGTTCATGTTGCTGAAAGCT750.388661449966316No Hit
GAATAGGGGTGATGTATATTTACTGATTTTGTACAATAATATATAAACATAT740.38347929730009844No Hit
CTACACGGGAGCCTTGCCTTGTGGAATGAGAAATATCTTCCTTTTAAAGGTA720.37311499196766335No Hit
CTCGCCTGGGTGCAGCAAATTTGTTCTTTTGGTTTCATCACTAACAAAAGTG720.37311499196766335No Hit
GATTAAGGGACAAATAGAAACATTAAAGCACTTTTAACTGATGATTTTCTAA710.3679328393014458No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGCCGTCT680.3523863813027932No Hit
GTTTTAGGGATTACAAGTGGGTAGGAAGGTATAAGGATATCATAGTGGGAAG680.3523863813027932No Hit
TACTCAAGTACCTAGACTCAGATACAGAGAAGGAACTGCAAGCACTTTATGC680.3523863813027932No Hit
GTTGATGGGAGCACAAACTGTGCAGTTTAGTTAATAGTAGCATAGCAATACT660.34202207597035805No Hit
TCGCCGGGGATGGTTATGCAGTGGTATCGTTTATATAGGAAAATCATGATAA650.33683992330414053No Hit
CTGTACGGGGATCTTAATGCGGGGACGCTCTTCCGATCTCGCGGTGGGCGAT640.331657770637923No Hit
GCTTCAGGGAACCCAAGTACGTTTTCTTATTTAAATGACAACTTTGGTGCTT640.331657770637923No Hit
GAGTACGGGTGAGGCAAGTGCATAGAAGGAGATGGGTTCAAAGAAGTAATTC640.331657770637923No Hit
TAGTATGGGGAAGCAAGCCTTTTGTCCTGAGAAGACTCTTTAATGTATATTT620.3212934653054879No Hit
TCAGAGGGACAGAGCTGGAGAAATACCCTTTATTGGAAAGCCATATGGATTG610.3161113126392704No Hit
GCTTATGGGAACCAAAAAACAGACAACAAAAGCAACCTGAGGAGAGGTTTCT600.3109291599730528No Hit
GGCGGCGGGCAGAGGACTACTACAGATGCAAAATCACCCCTTCTGCAAGAAA590.30574700730683524No Hit
AACAGTGGGCTTGGGATGGGGTCCTTTTGCGGGTGGCGGCGAACGCGGAGAG590.30574700730683524No Hit
CTCAAAGGGGTGCCATTGCACTCCAGCCTGGGCAATACAGTGAGACTCAGTC580.3005648546406177No Hit
AAGTTTGGGGTGTTCAAATCCTTTTGTTTTCTTATTAAGTCAGATGCTACTG580.3005648546406177No Hit
AGCAGCGGGAACTGCAGCTCTTTAAGAAGTTTTTTTTTTTTAGCTTCTAGGG570.29538270197440014No Hit
GCACCCAGGGGTTCTGCCAAGTTCCAATCACCAAGATCATGATTATGAATCC570.29538270197440014No Hit
CAAGGCAGGGGATCTCGGTTTGGGTAAGTCTCAAATTATCTCAACAAAATAA570.29538270197440014No Hit
GAACCGGGGATGCAAGAATTTAAAAGGAATGAACTGGAGCTGCAAGTGTCAA550.28501839664196504No Hit
GCTCAAGGGGGAGTGAGGACGCATGCGCGAGAGGACCCGCCGGGGGTTCAGC550.28501839664196504No Hit
GCAAGTGGGAAATATTACATTGTAGTGTTTCTAAAGAGTGCTTTGAAACTCA550.28501839664196504No Hit
GCGCTTGGGCACTTGTTAAGGTGGCACTTGCGTACTGAAACTTAATGATTCT550.28501839664196504No Hit
TAATAGGGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTAC540.2798362439757475No Hit
CATCATGGGGTGGGCCGGTCGCGGTGGCTCCCGGCTGTAATCCTAGCACTTT530.27465409130953No Hit
GTGCCAGGGGGGGATTGGTGAGCCCGTCTCTACCTGCGGGCGATGCATCCGT530.27465409130953No Hit
GCATCCGGGGAGATGAGATGAAATGAGATGCAATAATGAGATGAAATGCGAT490.25392548064465975No Hit
TGTAAGGGGGTGGGAGTAAAACTTTAATGGGAGGGGAGGGCTGGCTGCTGGA490.25392548064465975No Hit
TCATACTGGGAAATGAATTCAGAACATATTCCCTTCTCTTTCTCCCAGGGCT490.25392548064465975No Hit
AGAACAGGGATAACAATACTAAATATCACACTGTGATGGAGTTCCCAAATAT480.24874332797844226No Hit
CGTTGAGGGATCCATTGTACTGGAAAGAAACCCCGAGCTAGAATTTCAAGAC480.24874332797844226No Hit
CCAGCCTGGGTGACAGAGTGAAACTCTGTCTCAAAAAAGAAAGCCTGTACAT470.2435611753122247No Hit
CTTACGGCGGGTGTCACAATAACAGAACATGCAAGAACAATCATTATTTTAT470.2435611753122247No Hit
CTATGGGGGGGGAGAGAAGCCTTATCAGTGCAAGGAGTGTGGGAAAAGCTTC450.2331968699797896No Hit
GGACGGGGGGAGATTTTAATGAGTTATCACTTTTTCAGCTGATATATTCATT430.22283256464735451No Hit
CGAAATGGGGGGTCACATCTGAGTTATATTTCAGAGGTGGCATTGTCAATCG420.21765041198113697No Hit
ACGGCTGGGAGAGTCAGAGACTAATGGTGGCTTAAATGCACGAATGTCTTTT400.20728610664870187No Hit
AAGCCAGGGGAATACGAGGAGATTCTGAGACGCCCATCCCTGCAACGTCGAG400.20728610664870187No Hit
GAGCTGGGGAATCCCATTTTACCTCTAGACCTATAAGTTCTGGTTTATGTTT400.20728610664870187No Hit
GCAAAAGGGGTTCTAAATAATTTTTACAAGTTATGGTACAGACAGTTGTTAC390.20210395398248432No Hit
TACACAGGGATTGCATGCAAGTTTGCTGAGCTGAAGGAAAAGATTGATCGCC380.19692180131626677No Hit
GTACATGGGAACGAAGAGATGGAGCAGCCTATGCAGAATGGAGAGGAAGACC370.19173964865004922No Hit
TAACTGGGGTTTGTATAAATATGTTGTAAGGTGGCATTTGAAGTTCAGATAG360.18655749598383167No Hit
ATCTGAGGGCTCGTAGTGTCAGCTAATTATTTTGTATCCGGACTGTTTTATT350.18137534331761415No Hit
CCTTCCGGGTGGATTACCTGAGGTCAGGAGTTTGAGACTAGCCTGGCCAACA350.18137534331761415No Hit
ATATGGGAAGCAGTGATTTATCTTATCAATATGTAGACCTTATTAATGCTTT340.1761931906513966No Hit
CGCATAGGGATATTTAGATGTCTTAGTAGAGCGTATTATCATTTAAAGTGTA330.17101103798517903No Hit
AAATTAGGGAAGAGCATTTGTACTGCTGTGCTAGATAAGCTTTATGAATAGT330.17101103798517903No Hit
GGAACGGGTCCCACATTACTTTCTTTCAAAATCATATTAGCTTTCTAGTTTC320.1658288853189615No Hit
TTTAGCGGGTTCTGCAAGTTGATGAATCACAAAGTATCAAGAATGAGCTCAC320.1658288853189615No Hit
GACACTGGGATGGAAATTTTGTTAGTAGTTTGGTAAATGGTCCATTTTTATA320.1658288853189615No Hit
CAGTGCGGGACGCTCTTCCGATCTCTGTTTAAGGGATCTGTAATTTAAAGAT320.1658288853189615No Hit
AATTAAGGGAAGAGATGATTTATTTTATGTAAACATCTTTGCAAAGCAAGGT320.1658288853189615No Hit
ATTCTAGGGGAGGAAAAACAAGTATTATTAACATTAGCTCATTTCGGTAAAC320.1658288853189615No Hit
AAAATAGGGGATTCTCTAAGGTAATGGGGTGCTGCAGTGGGTGTAGCATTCA310.16064673265274396No Hit
TAAGGGGGAATGAGCACAGTCATCTATTCATGAAGCCTGACAAACCTTACAA300.1554645799865264No Hit
CTTAGTGGGACCTTTCAGAAACTTTTATGCTTTCTTCTGTCTCTCCATTTGT300.1554645799865264No Hit
TCCACGGGACGTTGCTAAAGATTTCTCTATAATAAGCCACACATTATATTTA300.1554645799865264No Hit
TAAGGAGGGAAGCACTTTCCAGACAACAGAAATAGAAGAAGTAAAAACACTG290.15028242732030886No Hit
TATACAGGGGCCTTAGCTGTTGAAGGAAAACGAATTGGAAAGGTGCTTGACA290.15028242732030886No Hit
TAAACAGGGAAGGCAGAACACTTACAGGAGTCCCTAACTGTGCCACCCTTGG290.15028242732030886No Hit
CACAAAGGGAAAAAAAAATGGAGAAAAAATTATAGGAAGAATTTTTAATAAT290.15028242732030886No Hit
GGATGCGGGTTTGCTCTGCTGCTTGAGATAATGGAGAGCAGGTTCTTTTAAC290.15028242732030886No Hit
TGGCTGGGGGTTGACAATTAGATTCTTGGACCAAGTGAGGGTGTACTCTGAT270.13991812198787376No Hit
CTTGAGGGGGTGTACGGAGTCGTATCTCCAACCTGAAGGATGCCAAGAACCC260.1347359693216562No Hit
TGCTTTGGGAGAGCGGGAGTCCATCATGAAGGAGTTCCGGTCGGGCGCCAGC260.1347359693216562No Hit
CCAAAAGGGACATGGAGTGTCTGAATCAGAGACCACCAGAAAACCCAGACAC250.12955381665543866No Hit
AAGGCTGGGGAGCCTCCTGCAGGTGCAGGGCCGGCCCGGAGCCCCCTTCTAG250.12955381665543866No Hit
TTAGCTGGGTTGGCCAAACTGGTCTCGAACACCTGACCTCAGGTGATCTGCT250.12955381665543866No Hit
ACTACGGGGGCTGGCACTCAGTTTTCTCTCCTGTCATCATCTAAAGAAGGAT240.12437166398922113No Hit
AATCCGGGGGTTGGGGGTGAAGGTAGCTGGTTGTAGGTGTGCAGAGCAGGAA240.12437166398922113No Hit
AGACATGGGCAGACTGTAACACAGTTACAGCAGTTGCTTCAGGCGGTAAACC240.12437166398922113No Hit
TCGAACGGGAGAATAGCTTCTTACTGAATATGCAAAAGAATAATTCCTTGTT240.12437166398922113No Hit
TAGCGTAGGGCTTAGAAGAATTCTATGTTCTGAAATGCACACTTTTAGAATG230.11918951132300357No Hit
CGCTAAGGGGTTTGTTTTATTCACCATCATGTACCAAGTGCTTGGCACATAG230.11918951132300357No Hit
TCCGTTGGGGCTTTTTCGTCTGGGCTGCCAACATGCCATCCAGACTGAGGAA230.11918951132300357No Hit
TCATGTGGGGGGTGCGCCCGCTGTCACCGCCATGGCTGCCCCGTGTTTGCTG220.11400735865678605No Hit
CTTGCTATGGGGTCCCTGGCATTTTAGGTGTCGGTTGGGTAGGCAGTCATGG210.10882520599056848No Hit
GTAAGTGGGTATTCCCCTCTGCCATACTATACATTCTTTTCCATTGTTTGGC210.10882520599056848No Hit
TGACTGGGGTGCCTACTGCCTGGGGGCTCTAGTCATTCCAGTGGAAGACGAA210.10882520599056848No Hit
GCAGAGGGAGGAGTGGCAGCCAGATGGAGTTGGTTGAGGGTAAGGTAGGGAG200.10364305332435093No Hit
AGACTGGGACGGGAGACGCTGTGAAGGTACCAGGGAAGGAACGAGTGAGGTT200.10364305332435093No Hit
CGTTGCGGGAGGTTGTACAGATTGAAATTTAGTTGTTTCATTGGCTGTCTGA200.10364305332435093No Hit
TGCTATGGGCCCATCGACTACAAACGGAACTTGCCGCGTCGAGGACTGTCGG200.10364305332435093No Hit
GACTACGGGATTGTTATTTCCACTTTCTCCAATACTTGTATTTTATGCTACA200.10364305332435093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)