FastQCFastQC Report
Sat 31 Dec 2022
SRR5844507.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844507.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19823
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1940.978661151188014No Hit
GGTCAGGGGGCAGAGCTGATTGGGAGGCTGGAGAGCAAAGCACTGTGGTTCG1830.9231700549866316No Hit
GAACAAGGGCCGCACTAAGGCCGGCTCTTGTGCCGGAAGGAGGAAGGCGTGG1780.8979468294405488No Hit
TAAGCTGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT1770.8929021843313323No Hit
TTAGCGAGGGGAGGAAGGGAGGCGGCGGCGCGAGGAGGAGGGAGCGGCCGTG1690.8525450234575997No Hit
CGTTAAGGGCCCAAATCGATGGAGCCACATTAATAATACTTTTCATGCCTCA1660.8374110881299501No Hit
GAATATGGGGGCCTGTTTGGATTGGAAGAGGAGATCTTACACAGAGGAACTA1650.8323664430207335No Hit
GACTCAGGGGGGAGCCGCCGGGAGTGGACGCCGCCGAGGCCCGGAGTCGCGC1640.8273217979115168No Hit
TAACGTGGGGCACCTCCCGCATGGCCCCTCCGGCCAGCCGGGCCCCTCAGAT1580.7970539272562175No Hit
CACCGGGGATGTTTGTAGGGGGCTGTGTGGCTTTTATCCTGGATAACACCAT1570.7920092821470011No Hit
TACAATGGGCACACTCACTACCTTGGGTTTGAAAATGGCTCAACCACCATCA1560.7869646370377844No Hit
TTCTAAGGGGGACCTCCTCCCCCAGGCTTTAAGTCTGAGTTCACAGGACCAC1540.7768753468193512No Hit
TGGCACGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1510.7617414114917015No Hit
AATCATGGGAGTCAGATGCTGTGGTACTTGGCATTTAACAACTAGAATTCAG1480.7466074761640519No Hit
GTGTTGGGGAGGGAGAAGATATTTAAGTGTCCAGAATGTGGGAAAACCTTCC1450.7314735408364021No Hit
TCTATGGGGAGGTGCAGGGGCTGCCCAGGACAAAGCGGGGGCAGGGGAAAGA1450.7314735408364021No Hit
TCTAGCGGGATTTGTTGGTGGCATTAAAGAAGACACTGAAGAACATCACCTA1440.7264288957271856No Hit
TAAGTTGGGCCACTACGCCTGGCCCTGCTCTGGTTTTTATTCCCCTTTCTTT1420.7163396055087524No Hit
TTTCTCGGGCCCAGACTATAATAATCCTGACTATGTAGACGGAAGTTTCTAT1420.7163396055087524No Hit
AAAATGGGGATAACCATGCACACTACTATAACCACCCTAACCCTGACTTCCC1410.7112949603995359No Hit
TAAGAGGGGAGGCGGAGAGAGGCGGTAAGCCGCGAGGAGGAAAGGGACTCAC1400.7062503152903193No Hit
CTTTGTGGGAGTTGATTCAGCGGTTGCGAAGAATGGAACGAAGCTTAAGTAG1390.7012056701811028No Hit
CATGATGGGATATCAGTATATAATGTTTTGAGACTATTATTACTCAATGTTA1380.6961610250718863No Hit
GGCGATGGGAGATTTAGAAGAAAAGATCCTGTTTCCATTTGAAAGGAACTGT1370.6911163799626696No Hit
TCTCTAGGGACCGAGGAGTTAAAGGATTTGTCCGAGACCTTCAAGGTAACCC1340.6759824446350199No Hit
ATCATCGGGGGGCTTCAGAAGTATCTGCACAATTAGAAAAGTCCTCAGAAGC1300.6558038641981537No Hit
ACTCACGGGAGAGCTGCCACCCTCACCTGTCCCCAAGCCACCCTGTGTCCCA1280.6457145739797205No Hit
GCACCTGGGATATAATGTCATGTAAAACTTTGCTTAAGATGGTCTGGTTTTT1280.6457145739797205No Hit
CAACAGAGGGGATCTGCAAATGGGGCTCACTCGCTCTGCCATGGTGCCATGA1270.640669928870504No Hit
TCTCAAGGGACTCAGGCTTGAGGCACCCGAATAAACAAAACTTAGAATTTAA1270.640669928870504No Hit
GACACAGGGTGTCTGTGTGGAGCACGTAGTGCACAGAATACGTGAGAGTTGC1270.640669928870504No Hit
TAAGCTGGGGGCTCTCTGCTCCTCCTGTTCGACAGTCAGCCGCATCTTCTTT1250.6305806386520708No Hit
CAGTCTGGGCAACAGACATCGCTATTGCGGTTCCGAGGCAGTGGGAAGAGAT1230.6204913484336377No Hit
ATGCACGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1230.6204913484336377No Hit
GCTAAAGGGACTAATATATTGACTATATTTAATAATAAATTGTCAGATAAAA1220.6154467033244212No Hit
TTAGACGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1220.6154467033244212No Hit
CCATGTGGGGAGCGCAGGGGTCACTGCACGCTGCAGGGCTCTTGCTGCCATG1210.6104020582152045No Hit
GTAGAAGGGACAAGCTTTCCAGAAGCAATTTTATAACAGATTTCATTTTAAC1200.605357413105988No Hit
AATATAGGGATGTGAAATTATGCAATTTGACTTGATTCCTGAACTTCGTGCT1190.6003127679967715No Hit
TGCATAGGGGCAGGCGCAAGCCGGCAAGATGGCGGCGGCTGGGGCTGGCCGT1180.5952681228875548No Hit
ACAATCGGGGCATTTTATAATAAAATGACAAACCAATTTTTTAAGTGAGCAA1180.5952681228875548No Hit
AGCCTGGGGGAGACCTTCGCATCGAGGGACGTGAGGGACATTTGTCCTAGAG1170.5902234777783383No Hit
GTAGACGGGACATGTAAGCATGAAGGCTGGCTCGTTGGTGAGCCATTGCTTT1150.5801341875599051No Hit
GTCAATGGGACTGGGACATGAGTGAGCAGGAGGTACTAAATGCAATAGAGCA1130.570044897341472No Hit
AGTACTGGGAAGTAGAGGTTATTCTTCAGCATAGTGCTGCTGACATTGCCAG1130.570044897341472No Hit
GTTTACGGGGTAGTACCACGAGGGACGCCAACTCCCAGAGGAGTCCTGTCCA1120.5650002522322555No Hit
ATGCCTGGGGCTGTTAAAGTAGATGACATCATGTGTTAGCCTGTTCCTAATC1100.5549109620138223No Hit
AGTCCAGGGGTCTTACCTCGTTGTGGCGTCAGAACCGCCGTCGCTCGCTCCC1090.5498663169046057No Hit
ATCGTTGGGAGATTTGCTAAAGTGTTCATGCCAAGTTTATAACTGACAGCAG1090.5498663169046057No Hit
TACCTGGGGCTGAGAGAGGGAGGTTGTGAATGCAGGTTTTATCAGAACTATG1090.5498663169046057No Hit
CTTACGGGGACTTCAACATGTGATTCAGAAGAATCACCTGGATGATGAGACC1080.5448216717953892No Hit
TGCTGGAGGGACTCACCCTGCAGCTCTGACCTTTTTTCCCAAAGGGACTTAA1080.5448216717953892No Hit
TCACACGGGAAACCTGAAGGAGTTTGAAGGATTGTTGGCTCAGATGCGAAAG1040.5246430913585229No Hit
GGATGGGGGTCTGCGATAGGGATGTGAATTTTAAATTAGAATAACCGTCTTA1040.5246430913585229No Hit
CTGCATGGGTGGCAACGATAACCTTAGAACAACTCATTTCACAGACCACCTG1040.5246430913585229No Hit
GCTTTAGGGAGAACTGGATCTGTCGGAGCTGGAAGGCCTGGGTCTGTCAGAT1010.5095091560308732No Hit
AGTTGAGGGGGCTGCCCCCCAAAATATGCCAAGACCTTGTACCTGCTGTACG1010.5095091560308732No Hit
TCACGCGGGCTCATTCAAATTTAAAGATATCTTTCTCCTCTTGATCAATCTA990.49941986581244013No Hit
GCGCTTGGGCTGATGGTTAATTTTAGACATCTACTTGAGTGGATTAAGAGAC980.49437522070322354No Hit
GCAAGCGGGGAAGAGTTTGAGGAAGAATTTGAGGAAGAAGAAGGTGAGTTAG970.489330575594007No Hit
TCATCTGGGAGCCTACACAATTGTAGGTAGTGGCCTTGTCTTATTTACTAAA950.4792412853755738No Hit
CTTGTTGGGGTGATTGATGTATCTAATGGGAAAGTTCATGTTGCTGAAAGCT940.47419664026635727No Hit
TGCAAAGGGTGCAAAGGAACAGGAGTGAGTCGTGACTGACAGGGGTGGTTGA920.46410735004792414No Hit
AGCTTCGGGACCTATTGATGGGAACCCCCTAACCACAGCTGCAGGGCCAACA910.4590627049387076No Hit
GAAAAAGGGAGAGATTAATACAACTACTTAAAAAATATAGTCAATAGGTTAC900.45401805982949095No Hit
GGTTATGGGTACTAAAACTTTATAAAAATGTAAGTAATTATGTTGCCAATTT860.43383947939262474No Hit
GTTGATGGGAGCACAAACTGTGCAGTTTAGTTAATAGTAGCATAGCAATACT840.4237501891741916No Hit
GCATCCGGGGAGATGAGATGAAATGAGATGCAATAATGAGATGAAATGCGAT840.4237501891741916No Hit
GCACCCAGGGGTTCTGCCAAGTTCCAATCACCAAGATCATGATTATGAATCC830.41870554406497507No Hit
ATGGTTGGGCCTTAATAATATAGTTTCTACAGAGCTTTCTTACTTATTTTTC800.40357160873732534No Hit
AGTGAAGGGATCTTTGATGAGGGCAGAGCTCACGTTGCATTGAAGACGAAAC800.40357160873732534No Hit
TCACAGGGATAAATTGAAAGGGCAGGTGGACAGAAAGAAGAGACTGACTCCT790.39852696362810874No Hit
CTACACGGGAGCCTTGCCTTGTGGAATGAGAAATATCTTCCTTTTAAAGGTA790.39852696362810874No Hit
GCTTATGGGAACCAAAAAACAGACAACAAAAGCAACCTGAGGAGAGGTTTCT770.3884376734096756No Hit
ATTTCAGGGATCTATCTAGATTTCTGAACCAAGATATATTTATAGTTCACTT760.38339302830045907No Hit
GTTTTAGGGATTACAAGTGGGTAGGAAGGTATAAGGATATCATAGTGGGAAG760.38339302830045907No Hit
AACAGTGGGCTTGGGATGGGGTCCTTTTGCGGGTGGCGGCGAACGCGGAGAG740.37330373808202594No Hit
GTGCTAGGGATCTCACATATTTTCCAGACACACTTATATCATTAGTCCATAG740.37330373808202594No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGC720.3632144478635928No Hit
TCGCCGGGGATGGTTATGCAGTGGTATCGTTTATATAGGAAAATCATGATAA720.3632144478635928No Hit
ATCTATTGGGGATGATAGCACGAGTTTGTTTTTCAATTTTGCAAGATAATTT720.3632144478635928No Hit
ACGGCTGGGAGAGTCAGAGACTAATGGTGGCTTAAATGCACGAATGTCTTTT690.34808051253594313No Hit
GAATAGGGGTGATGTATATTTACTGATTTTGTACAATAATATATAAACATAT690.34808051253594313No Hit
GGCGGCGGGCAGAGGACTACTACAGATGCAAAATCACCCCTTCTGCAAGAAA680.34303586742672654No Hit
TACTCAAGTACCTAGACTCAGATACAGAGAAGGAACTGCAAGCACTTTATGC680.34303586742672654No Hit
GTATAGGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGC680.34303586742672654No Hit
TCAGAGGGACAGAGCTGGAGAAATACCCTTTATTGGAAAGCCATATGGATTG670.33799122231750994No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGCCGTCT670.33799122231750994No Hit
CAGTAAGGGCATGATGACCTAGGAGAGTTTCCATATGGATTGTTTGAACTTG660.3329465772082934No Hit
TAGTATGGGGAAGCAAGCCTTTTGTCCTGAGAAGACTCTTTAATGTATATTT650.32790193209907686No Hit
GCGCTTGGGCACTTGTTAAGGTGGCACTTGCGTACTGAAACTTAATGATTCT650.32790193209907686No Hit
GATTAAGGGACAAATAGAAACATTAAAGCACTTTTAACTGATGATTTTCTAA640.32285728698986027No Hit
GTGCCAGGGGGGGATTGGTGAGCCCGTCTCTACCTGCGGGCGATGCATCCGT630.3178126418806437No Hit
TAATAGGGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTAC620.3127679967714271No Hit
CTCAAAGGGGTGCCATTGCACTCCAGCCTGGGCAATACAGTGAGACTCAGTC610.3077233516622106No Hit
CTCGCCTGGGTGCAGCAAATTTGTTCTTTTGGTTTCATCACTAACAAAAGTG610.3077233516622106No Hit
GCTTCAGGGAACCCAAGTACGTTTTCTTATTTAAATGACAACTTTGGTGCTT600.302678706552994No Hit
GAGTACGGGTGAGGCAAGTGCATAGAAGGAGATGGGTTCAAAGAAGTAATTC590.2976340614437774No Hit
CTTACGGCGGGTGTCACAATAACAGAACATGCAAGAACAATCATTATTTTAT580.29258941633456087No Hit
GAACCGGGGATGCAAGAATTTAAAAGGAATGAACTGGAGCTGCAAGTGTCAA550.27745548100691114No Hit
CATCATGGGGTGGGCCGGTCGCGGTGGCTCCCGGCTGTAATCCTAGCACTTT540.2724108358976946No Hit
AGCAGCGGGAACTGCAGCTCTTTAAGAAGTTTTTTTTTTTTAGCTTCTAGGG530.267366190788478No Hit
TGTAAGGGGGTGGGAGTAAAACTTTAATGGGAGGGGAGGGCTGGCTGCTGGA530.267366190788478No Hit
GCTCAAGGGGGAGTGAGGACGCATGCGCGAGAGGACCCGCCGGGGGTTCAGC530.267366190788478No Hit
CAAGGCAGGGGATCTCGGTTTGGGTAAGTCTCAAATTATCTCAACAAAATAA520.26232154567926147No Hit
GCAAGTGGGAAATATTACATTGTAGTGTTTCTAAAGAGTGCTTTGAAACTCA520.26232154567926147No Hit
CTGTACGGGGATCTTAATGCGGGGACGCTCTTCCGATCTCGCGGTGGGCGAT510.25727690057004493No Hit
AGAACAGGGATAACAATACTAAATATCACACTGTGATGGAGTTCCCAAATAT490.24718761035161177No Hit
CTATGGGGGGGGAGAGAAGCCTTATCAGTGCAAGGAGTGTGGGAAAAGCTTC490.24718761035161177No Hit
ATCTGAGGGCTCGTAGTGTCAGCTAATTATTTTGTATCCGGACTGTTTTATT480.24214296524239518No Hit
AAAATAGGGGATTCTCTAAGGTAATGGGGTGCTGCAGTGGGTGTAGCATTCA480.24214296524239518No Hit
TACACAGGGATTGCATGCAAGTTTGCTGAGCTGAAGGAAAAGATTGATCGCC470.23709832013317864No Hit
AAGTTTGGGGTGTTCAAATCCTTTTGTTTTCTTATTAAGTCAGATGCTACTG460.23205367502396207No Hit
CCAGCCTGGGTGACAGAGTGAAACTCTGTCTCAAAAAAGAAAGCCTGTACAT450.22700902991474547No Hit
CGAAATGGGGGGTCACATCTGAGTTATATTTCAGAGGTGGCATTGTCAATCG440.22196438480552894No Hit
GCAGAGGGAGGAGTGGCAGCCAGATGGAGTTGGTTGAGGGTAAGGTAGGGAG430.21691973969631237No Hit
AAATTAGGGAAGAGCATTTGTACTGCTGTGCTAGATAAGCTTTATGAATAGT430.21691973969631237No Hit
CGTTGAGGGATCCATTGTACTGGAAAGAAACCCCGAGCTAGAATTTCAAGAC420.2118750945870958No Hit
GACACTGGGATGGAAATTTTGTTAGTAGTTTGGTAAATGGTCCATTTTTATA420.2118750945870958No Hit
GGACGGGGGGAGATTTTAATGAGTTATCACTTTTTCAGCTGATATATTCATT420.2118750945870958No Hit
AATTAAGGGAAGAGATGATTTATTTTATGTAAACATCTTTGCAAAGCAAGGT420.2118750945870958No Hit
TAAGGGGGAATGAGCACAGTCATCTATTCATGAAGCCTGACAAACCTTACAA400.20178580436866267No Hit
GTACATGGGAACGAAGAGATGGAGCAGCCTATGCAGAATGGAGAGGAAGACC400.20178580436866267No Hit
CGCATAGGGATATTTAGATGTCTTAGTAGAGCGTATTATCATTTAAAGTGTA390.1967411592594461No Hit
CAGTGCGGGACGCTCTTCCGATCTCTGTTTAAGGGATCTGTAATTTAAAGAT380.19169651415022954No Hit
ATATGGGAAGCAGTGATTTATCTTATCAATATGTAGACCTTATTAATGCTTT370.18665186904101297No Hit
CCTTCCGGGTGGATTACCTGAGGTCAGGAGTTTGAGACTAGCCTGGCCAACA370.18665186904101297No Hit
TTTAGCGGGTTCTGCAAGTTGATGAATCACAAAGTATCAAGAATGAGCTCAC370.18665186904101297No Hit
CCAAAAGGGACATGGAGTGTCTGAATCAGAGACCACCAGAAAACCCAGACAC350.17656257882257984No Hit
AAGCCAGGGGAATACGAGGAGATTCTGAGACGCCCATCCCTGCAACGTCGAG340.17151793371336327No Hit
TCATACTGGGAAATGAATTCAGAACATATTCCCTTCTCTTTCTCCCAGGGCT340.17151793371336327No Hit
AAAGCGGGTCTCCGTCGCCATGGAAACGAAAGCGGCCAAGTAGAGCTCCGTC340.17151793371336327No Hit
GCAAAAGGGGTTCTAAATAATTTTTACAAGTTATGGTACAGACAGTTGTTAC340.17151793371336327No Hit
TGCTATGGGCCCATCGACTACAAACGGAACTTGCCGCGTCGAGGACTGTCGG330.1664732886041467No Hit
CACAAAGGGAAAAAAAAATGGAGAAAAAATTATAGGAAGAATTTTTAATAAT330.1664732886041467No Hit
TCCACGGGACGTTGCTAAAGATTTCTCTATAATAAGCCACACATTATATTTA320.16142864349493014No Hit
TTAGCTGGGTTGGCCAAACTGGTCTCGAACACCTGACCTCAGGTGATCTGCT320.16142864349493014No Hit
GGAACGGGTCCCACATTACTTTCTTTCAAAATCATATTAGCTTTCTAGTTTC310.15638399838571354No Hit
AATCCGGGGGTTGGGGGTGAAGGTAGCTGGTTGTAGGTGTGCAGAGCAGGAA310.15638399838571354No Hit
AAGGCTGGGGAGCCTCCTGCAGGTGCAGGGCCGGCCCGGAGCCCCCTTCTAG310.15638399838571354No Hit
TCGAACGGGAGAATAGCTTCTTACTGAATATGCAAAAGAATAATTCCTTGTT310.15638399838571354No Hit
TAAACAGGGAAGGCAGAACACTTACAGGAGTCCCTAACTGTGCCACCCTTGG290.14629470816728044No Hit
TCCGTTGGGGCTTTTTCGTCTGGGCTGCCAACATGCCATCCAGACTGAGGAA290.14629470816728044No Hit
AGACTGGGACGGGAGACGCTGTGAAGGTACCAGGGAAGGAACGAGTGAGGTT280.14125006305806387No Hit
ATTCTAGGGGAGGAAAAACAAGTATTATTAACATTAGCTCATTTCGGTAAAC280.14125006305806387No Hit
AGCTCAGGGGCTTTTTAAATTTTCCATTAATTTCAACATAATTATGGGAACA270.1362054179488473No Hit
TTATGGGGGAAGTTGATTTTTAAAGGCAGCATCAAAAACTGAAAAGGAAGGG270.1362054179488473No Hit
GAGCTGGGGAATCCCATTTTACCTCTAGACCTATAAGTTCTGGTTTATGTTT270.1362054179488473No Hit
TGACTGGGGTGCCTACTGCCTGGGGGCTCTAGTCATTCCAGTGGAAGACGAA260.13116077283963073No Hit
CTTAGTGGGACCTTTCAGAAACTTTTATGCTTTCTTCTGTCTCTCCATTTGT260.13116077283963073No Hit
CTTGAGGGGGTGTACGGAGTCGTATCTCCAACCTGAAGGATGCCAAGAACCC260.13116077283963073No Hit
TAACATGGGATTTAATTTTGATCTGAGACATTTTTCCAAGCTGTTAATTTCA260.13116077283963073No Hit
TAAGGAGGGAAGCACTTTCCAGACAACAGAAATAGAAGAAGTAAAAACACTG250.12611612773041417No Hit
GGACATGGGTCCTAATTTAACGGTGCAAATATTAATGTATATACTGTACAGT250.12611612773041417No Hit
TAGCGTAGGGCTTAGAAGAATTCTATGTTCTGAAATGCACACTTTTAGAATG240.12107148262119759No Hit
AGTGCGGGGTGTGACTGACCATGTCTGACTGCCACTCCTTTTTGCAGGAGCA240.12107148262119759No Hit
TAACTGGGGTTTGTATAAATATGTTGTAAGGTGGCATTTGAAGTTCAGATAG240.12107148262119759No Hit
TATACAGGGGCCTTAGCTGTTGAAGGAAAACGAATTGGAAAGGTGCTTGACA240.12107148262119759No Hit
TGCATAGGGATCCTCGTGCACAGAAATGCTCAGGGTCCCCATGTGCCTGTTG240.12107148262119759No Hit
TGCTTTGGGAGAGCGGGAGTCCATCATGAAGGAGTTCCGGTCGGGCGCCAGC230.11602683751198103No Hit
AGTCTGGGGATGTTCATGATATATATTAGTGTCTGCATATATATACTGCAAA220.11098219240276447No Hit
GCTTCCGGGAGCACTTTGAAAACAACACAGTTTTCTTGTACCCTGGGAGAGA220.11098219240276447No Hit
CTTTTAGGGGGAGCCTCCCAGAGGCCCAGAGCCTGTGGCCAAGGCCACTGTT220.11098219240276447No Hit
ATTAGGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAG220.11098219240276447No Hit
TAGATTTGGGGTGATTATAACAGAAAATATGTAAATAGTGCCTACACTGATT210.1059375472935479No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)