FastQCFastQC Report
Sat 31 Dec 2022
SRR5844504.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844504.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59012
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGCCGTCT2540.4304209313359995No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGC2270.3846675252491019No Hit
TCGCATGGGGGGGAACACTATGAGTTTTCTCTTTCTTTTTATTTTTTCAGTT700.1186199417067715No Hit
TTTCCTGGGCGACCCCCCAAAGTGCTGATTACAGATGTGAGCCACTGTGTCT650.11014708872771639No Hit
ATTTGGGGGATTCTTGAGCCTTGACCTTGGTTATTTGTTCTGGTGTTCTGTG640.10845251813190537No Hit
AGTACAGGGGAAGCTCTTTTCTGTAGAGTATTAAATTGTGAAATCAGTAATA620.10506337694028334No Hit
ATCATGGGACCACTCTTCTGGTTCTGAAAATGAAGAGAAAGCCATGAGTAAA620.10506337694028334No Hit
TTGCTACGGGTGCTTTCCTATGGAGAAACCAGGCAATGTTATTCATCCTCAA620.10506337694028334No Hit
TTCAACGGGTACACGACGCTCTTCAGATCTACTAGTGGGGGATCATGGAAGA620.10506337694028334No Hit
GCTGCAGGGACAAGTGGTATTGCTGAATCCTGAAATATGCATGTGTTTAAAT600.1016742357486613No Hit
AATCAAGGGGACCTAAATGTAGGAGTTAAAACTATGAAACATGTAGAAGAAA600.1016742357486613No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)