FastQCFastQC Report
Sat 31 Dec 2022
SRR5844501.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844501.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65875
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGC1750.2656546489563567No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGCCGTCT1610.2444022770398482No Hit
CCATAGGGACATAAGGACCCCAGCAGCAAGCCTGCCTGTGGACCATACCAAA970.14724857685009488No Hit
GCTAATTGGGAGATATTTCTCTGATAAATCAGGTAACAAAATGTATTTGATT810.12296015180265656No Hit
TAGACTGGTTTAGCACAAGCCATCCACATTAATTCAAACCTGTGGCTCTGAA800.12144212523719165No Hit
TTTAGCGGGAATGAGAGTGAAAATCAAGAGTGATTAAAGCATGCAAAGGGAA770.11688804554079696No Hit
CAGACAGGGTCTCTCAGGTGACTCTATCTTCTGGTTTGCCGGCCATCCCATA740.11233396584440228No Hit
GCGTGGGGGTTTATAGAGGAGAAAAATAGCTGAAAATTTTCAAAAGTGGCTA740.11233396584440228No Hit
TTGCTTGGGTACAACATGCATGACACTTTGCCATGAACTGGGGCTCTGGGAG740.11233396584440228No Hit
AATCAAGGGCTATCAAAAAGAATAAAATATTGAGGAACACACTTAACAGAAG730.11081593927893738No Hit
AAACTAGGGACTGTTGGGAAGGAATAGATCTTTTTGAACTAAACAGAGTAAA710.10777988614800758No Hit
TATTGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA710.10777988614800758No Hit
GCAAACGGGAAACTAGAGTGGAATGGATGAGCGCCTTGTAAAGAGACATCCA710.10777988614800758No Hit
AACAATGGGACTGCCTGGCTGGACCCTTTAGCTAGTTAGAGCATTTATTGCT710.10777988614800758No Hit
ACACAAGGGATGAGGAACCCATGGATGTGGTGGCACTGTTTGGTCTGAGGGG700.1062618595825427No Hit
TATAATGGGCACAGCCCTCATGAAGCTCAGCACTCGCCTCTGTGGGGACAAC700.1062618595825427No Hit
AGTGCAGGGATGGCAATAACTATATTAGATATAAAAGGTCTAAACACCCCAG700.1062618595825427No Hit
ACTTTGGGGGGAAAAAATGTCAATATGTGGGAAACTGTTCCTTTGCTCATAG690.1047438330170778No Hit
GCATCTGGGAACAGATTTCATTTTAACAAAATACTGATCCAATTTTCATTAT690.1047438330170778No Hit
GTGCGGGGGAAGAAGAAATCGAAGAGAGAATAGAAACAGTTAAAAAATTTTT690.1047438330170778No Hit
AGACGCGGGATTCCTCATGAAAACTCACCAGATCAACAGCAACCTGAGGAGC680.1032258064516129No Hit
GCTCATGGGGTCTCATTTTTGATGTTTATTTATTATGTTCATTTGAAAGTTA680.1032258064516129No Hit
TCTCTGGGGGGTTTTTTTTCTTAGCACAAAACCAACTTCCCAAATTACAACT680.1032258064516129No Hit
CCACCAGGGATGGTTTCCCATGAGACTCCTTCTCATACCTGAAGAGCTATTA680.1032258064516129No Hit
GCTCAAGGGATATTCTGTTGTGTATATACCCAGTAATGGGATTGCTGAGTTG670.10170777988614801No Hit
TGTATTGGGGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCAAAAGAAAC670.10170777988614801No Hit
TCTATTGGGCAAAGTTTGGAATATTTGCATTATACTTACCAGTTGGGCATCC670.10170777988614801No Hit
TAACCGGGGAAGGGGCATATTTCTACTTCAGGAAACTCAGCTTTGATCAGGC670.10170777988614801No Hit
ATGTTAGGGTGGTGACCTCCTTTAAGGAATCTTTGTGAACTGGGTTGGAAGT670.10170777988614801No Hit
ACTTTTCATTGGGAACTGAGACACTGGCTGTGAGCCTTTCTGTCCTGAGATG660.10018975332068313No Hit
ATTACAGGGGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTT660.10018975332068313No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)