FastQCFastQC Report
Sat 31 Dec 2022
SRR5844500.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844500.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63851
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGCCGTCT1740.2725094360307591No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGC1460.2286573428763841No Hit
CCATAGGGACATAAGGACCCCAGCAGCAAGCCTGCCTGTGGACCATACCAAA960.15035003367214297No Hit
TATTGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA780.12215940235861615No Hit
TATCACGGGAATCTCAGGCCTCATGAAGCTTATATACCATTAGTATAGTTTT780.12215940235861615No Hit
GCTGCGAGGGAGATCAAAGCTAAGTGTGGAGAATAAGGGCTGCTTCTCCTTT740.11589481762227687No Hit
GCACAGGGGTCAAGAAAGCTCAGCTATAAAAACAGACTAGAAATCCAGGCAG740.11589481762227687No Hit
GCTCATGGGGTCTCATTTTTGATGTTTATTTATTATGTTCATTTGAAAGTTA730.11432867143819204No Hit
CATTCCGGGGATCTGCTAGGGGGGTAGTGGAGGTTTTACATTTGTTTGCACA730.11432867143819204No Hit
GTATATGGGGTTATCTTCTCTAGGAACTTTTCCCTAATAGCCGCCTCTACCT720.11276252525410722No Hit
TGACGCGGGAGAAAGCGTTATTTAATGAATTCAAATTCACAAAACTGCTTCT720.11276252525410722No Hit
TCTGCTTACGGGATGATGGAATTTATCATAAAGCTTTGTGATTAGTTTGTAC710.11119637907002239No Hit
GTTCATGGGGTGATAGTTTATGCATCACATAAAATTTGCCATATTAACCAGA710.11119637907002239No Hit
GTTTACGGGGGAAGGAAAAATGTAAGGTTTAACAGTAAGCACTTGCATTGAA670.1049317943336831No Hit
ATACGCGGGAAATAGGAGTCATGGGCGTTAGTTCTGTAGTAATAACTTCCCA670.1049317943336831No Hit
GCTAATTGGGAGATATTTCTCTGATAAATCAGGTAACAAAATGTATTTGATT660.10336564814959828No Hit
CAGTATGGGCTTATGAGATTAGTTGTTTCAAGTAGGAGACAGAAAAGTTCTG660.10336564814959828No Hit
CTACTCGGGAGCATATGTAGCGTTAATAGATCTGTCCTGGTAACTGTGTCTT660.10336564814959828No Hit
CTCTAGGGGCTAGGTGATGCAGCAGTAACAAACAACCCCGAGATCTTGGTGG650.10179950196551346No Hit
CCACCAGGGATGGTTTCCCATGAGACTCCTTCTCATACCTGAAGAGCTATTA650.10179950196551346No Hit
AACCAGAGGGCAAATTTAAGATAATGCAATGTAAGTGTTTGCTACCAAAAAT650.10179950196551346No Hit
TTAAAGGGGATCTACTCATTGTAAAAACTCAAACAGTGCAGAAATGTAGAAA650.10179950196551346No Hit
TTTGTTGGGAAGATTGTTCTGCTTATTCTTTGAAATAGGTTTTAATAAAATG640.10023335578142863No Hit
TCGCACGGGGGCAGAGCCGGGTGGAGCCCATTGACGTCCAGCGAAGCGAGGA640.10023335578142863No Hit
AATCCAGGGCTGCAAAGCACTGCTCGAAGACTTTTCTCTCAATGAAACTCGC640.10023335578142863No Hit
CAGTGAGGCTAGTGACTAGTTTACTAACTAGGTCATTCAAGGAAGTCAAGTT640.10023335578142863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)