FastQCFastQC Report
Sat 31 Dec 2022
SRR5844495.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844495.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208413
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGC3620.17369357957517045No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGCCGTCT2990.14346513893087284No Hit
ACCTGTGGGACTGGGAGTCAAAAAGAGTCAATGCTATTACTGGCATGTTAAA2840.13626789115842103No Hit
TAGCTGGGGCTCGCGAGACAAGCTCACCGAAATGGCCGCGTCCAGTCAAGGA2450.1175550469500463No Hit
CAAGCTGGGATTCTTCATTTATGATAACGGTATATTTTATGAAGAGGTTTCT2430.1165954139137194No Hit
CAAACAGGGAACTCCCAGTGGACTGCAGCCTGAAATAATCTGTCTTGTTACT2390.11467614784106557No Hit
TAAGAAGGGACGTTTTGAAATCACGTAGTCAAATTCTTGAGCTTTGCTCTTC2350.11275688176841177No Hit
CGTTCCGGGCTACTAGTTCAATGTGCTCTTTTTTACTTGACTCCAAATGACA2320.11131743221392139No Hit
ACTCTCGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA2310.11083761569575794No Hit
CTGAGTGGGGTTGGTAAGGAGTAGTGGTCAAGTCTACAGCAGTTCTACCTTC2300.11035779917759449No Hit
ATGTACGGGGGAATGAAAAAATCTAAGATTAGTTGAGTTCTTTGCTGCCTTC2290.10987798265943104No Hit
TTGTTGGGGAACCGTGGGCCTGTTACCTCCAGTACGAGCCATGAGCCAGCGG2290.10987798265943104No Hit
TTGACGTGGGACTCTTGTCCTTTTGATGGCATGATAACAGAAACTAAGGGAA2280.10939816614126759No Hit
TTTCGAGGGGTCATTTCTTTCTCTGAGTTGTACATTCTTTAAATAATACCAA2250.1079587165867772No Hit
GACCATGGGACTTGGGAAAAGAACATTTCTGATGAATGAACTAAAGAGGTGA2250.1079587165867772No Hit
TCTACAGGGATTTTCTTCCCAGATAAAACAATAAAATTATGGAAAATCAGTG2220.10651926703228685No Hit
GCTACTGGGCATGTCACTTGTGCTTTAAAATTAGTCTGTATCACCATTTATT2220.10651926703228685No Hit
TTCATCGGGATCCTTAAAAACAAAGCATCTATTTTGGCAGAAATTGTGTTCT2180.10460000095963302No Hit
GTAGGCGGGATGTTAGTGCATTAGAGAGACAGCAGGTTTCTTTGCTGGAGAT2170.10412018444146957No Hit
TAAGTAGGGGAGTAGGGAAAGACCCAGGCTGCGGGACGCGGTGCAGGCTGCG2160.10364036792330612No Hit
CCTTGTGGGACTCGTGGCAGGCATTGCTAGTCGATCCATGATACTCTTTCCT2150.10316055140514267No Hit
TTCTAGGGGAAAGTCTCCTATTTAAAAACCAGTTCAGGGAACACCAGACCTG2120.10172110185065232No Hit
CTTGTCGGGTGCCCAAGATATTTTAGAGCCATCCCATTCATAATAATCAATA2110.10124128533248884No Hit
TGGCTTGGGGGGAAAGGGTGTTTGGCCTTGACCAGCCACTGCTGACCTCAAT2090.10028165229616194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)