FastQCFastQC Report
Sat 31 Dec 2022
SRR5844494.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844494.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197694
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGC2940.14871468026343743No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGCCGTCT2830.14315052555970337No Hit
ATGTACGGGGGAATGAAAAAATCTAAGATTAGTTGAGTTCTTTGCTGCCTTC2420.12241140348214917No Hit
TTGTTGGGGAACCGTGGGCCTGTTACCTCCAGTACGAGCCATGAGCCAGCGG2330.11785891327000314No Hit
ACCTGTGGGACTGGGAGTCAAAAAGAGTCAATGCTATTACTGGCATGTTAAA2330.11785891327000314No Hit
TGCAGCGGGGAAATAAAAAAAGGACTTTTTTCCTTTTGCAATTACTATAAAA2300.11634141653262113No Hit
GTAGGCGGGATGTTAGTGCATTAGAGAGACAGCAGGTTTCTTTGCTGGAGAT2230.11280059081206309No Hit
TTCATCGGGATCCTTAAAAACAAAGCATCTATTTTGGCAGAAATTGTGTTCT2210.11178892632047507No Hit
CGTACAGGGACTCCATCTTGCCTGGTCCCCAGACACACAGGCCTTCTTTGGT2190.11077726182888707No Hit
CAAGCTGGGATTCTTCATTTATGATAACGGTATATTTTATGAAGAGGTTTCT2140.10824810059991705No Hit
TGGCTTGGGGGGAAAGGGTGTTTGGCCTTGACCAGCCACTGCTGACCTCAAT2120.10723643610832903No Hit
GACCATGGGACTTGGGAAAAGAACATTTCTGATGAATGAACTAAAGAGGTGA2110.10673060386253504No Hit
TACACTGGGAATTTTAGTGCATTTCTCCTCATCAGGAATGCTGGAGGTGCAT2090.10571893937094702No Hit
TTTCGAGGGGTCATTTCTTTCTCTGAGTTGTACATTCTTTAAATAATACCAA2070.104707274879359No Hit
CAAACAGGGAACTCCCAGTGGACTGCAGCCTGAAATAATCTGTCTTGTTACT2040.103189778141977No Hit
CCTTGTGGGACTCGTGGCAGGCATTGCTAGTCGATCCATGATACTCTTTCCT2020.10217811365038898No Hit
GCGCTAGGGGAAGACCAGAAAACCTGCTAGATAAATTCTAAGAGAGCTGTAA2000.10116644915880096No Hit
CTGAGTGGGGTTGGTAAGGAGTAGTGGTCAAGTCTACAGCAGTTCTACCTTC1990.10066061691300697No Hit
CTAGAAGGGATTTTAAAAACTTCACGTGTCTTAGTCTCAAAGTTATCAAAGG1990.10066061691300697No Hit
ACTCTCGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1980.10015478466721298No Hit

[OK]Adapter Content

Adapter graph