FastQCFastQC Report
Sat 31 Dec 2022
SRR5844482.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844482.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68141
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGCCGTCT2780.4079775759087774No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGC2070.30378186407596014No Hit
GATTTGGGGATCTCATTAACACAAAGAGGTCTGTGCCTGACCTAGGATCTTT1100.16142997607901263No Hit
TGGCTTGGGGGTTCTATGGTGGGAGAAGTGAACCTTTATTATCTGGGTATTC1020.14968961418235718No Hit
TACAGGGGGAGAAGTCAATTAATACAATCATTTTGTCTTAATTATTTAAAGT990.14528697847111136No Hit
GGTATGGGGGCGATCCTTTGGCCTCGGCCTCCCAAAGTGCATGAGCCACCAT980.14381943323402946No Hit
TTTGAGGGGACGTTCTCCTTTGTAAATTAGGGTGATCTTTTTTTTTTTTTTT940.1379492522857017No Hit
TCATGGGATTTGAAATGAGAAGTAGTATGTGTGCATATATTCATGTACGTGT900.13207907133737398No Hit
TTTGCTTGGGGAAATCAATCAAGATAACTTTATTCAAAGAGCAGCCCGCTTT890.13061152610029206No Hit
GTCGATGGGGATGAAGAAAGGATCCTAAAAGCAGAGAGAGAAACAAATACCA880.1291439808632101No Hit
TTAGATGGGATCTTTAATTAATGAGGTAATTCGGGCAAAGAGTAGAATTTAA870.12767643562612815No Hit
GTTTGTGGGATTCAAGTATTTGATCAGTAAGTCACAGTTATCTCCAGTGCAT860.12620889038904626No Hit
TAGCGTGGGCATCATTAAAATCTCTTTCCTGACCTTTGCTTTCTAGGTGATT860.12620889038904626No Hit
ACGCCGGGATAGCTGACCTTGAATGAGTTGTTTCATCTCTCTGCCCCTGTTT860.12620889038904626No Hit
ATCTCAGGGGACTCCACTAATAGAGGAATAAATGCCTAATAAAAAGAGAGCA860.12620889038904626No Hit
CTTGACCGGGATTCTGGAGAAATAAAGGCTTGATAGAAGAAAACAAGTCACT860.12620889038904626No Hit
CCACTTGGGATTACCAGAAGCTCATTATTTCTTCAAATATTTCCTCTGTTTT850.1247413451519643No Hit
AGCACTGGGCAAGCATTGATTGTGAATGTCATATCAAAGATTGGCTTTCTTA840.12327379991488238No Hit
ACTGAAGGGAAAGAGGACATAGACTATAGGCATTTTAAAGATGAAGAAATGA840.12327379991488238No Hit
CCTAGTGGTTTTTCTCTTGATACGTTCAAATGAAAAGGCCCGCATAGAAGCT840.12327379991488238No Hit
CAGTGAGGGGGATATAAAGAGTCAAGATTCAGGTGTGCTGTATTCAGGGTTC830.12180625467780044No Hit
AAATGTGGGGGCAGTGACATAGCTTATGAAAGGCACAAGTGGCACCAATAGA830.12180625467780044No Hit
AATGACGGCACAGACATCTGGGGGAAGAAAAGGAGCAGGAAACTACCCCGCA830.12180625467780044No Hit
CGTACCGGGTAAATGATCAAGAACAAAATTCATAAACTTACTTATTTGGATT830.12180625467780044No Hit
ATTTAGGGATCCACATGATGTGGTAGGTTTTTCTTTATTTTTACAACATGAT830.12180625467780044No Hit
GTGTCAGGGCTGGTAAAAATATTTCCTAGTGATATTTGTGATTTCTTCTTTG820.12033870944071852No Hit
TATCTGGGAGGACAAATCTTTCACCTGTTCCTTCATCCTTCAGTTCATCCAT820.12033870944071852No Hit
CTTACTGGGGGGTAAATACATAAAGGAAAAATCAAAAATGTGCATATTTTCT820.12033870944071852No Hit
TGTACTGGGAAGAATACTGATGTGACTGTAGAAATTATTGGCACATTTTTGA810.11887116420363658No Hit
ATAACAGGGTCTCTCCTCAAATCTCTGTAAGGAAGGTTTTATTGCTCTGTGA810.11887116420363658No Hit
AACGCCGGGGCATTACTTCGAAGCACGAAAGTGCTTTTCCATTATCAAAATT810.11887116420363658No Hit
TATAGGAGGGAGCTTTGCCTGTTTTGAACTTTAAATAATGTATATTCCCCAC800.11740361896655463No Hit
AGCTGAGGGTAAGGTACCTATACTACCTGTGAAAGGGTATAGTATTCTTTGA800.11740361896655463No Hit
TATGCTGGGCTCAAACAATAGCAAAAAGAACAATCCAACTTAAAAATGGCCA800.11740361896655463No Hit
CTCTTCGGGATATTACTATTGGGAATCTAAAATGATGTAGCTACTGAAGTTA800.11740361896655463No Hit
GTCAAGTCTGTCCCCAGGGGAGGTCCCCCTTTCGGGAGGAAGTTTTTAAGGG800.11740361896655463No Hit
TAGCGCGGGGACAGCACAGGAAAAACTACCATTTATAAAACCATCAGATCTC800.11740361896655463No Hit
CTTTATGGGACAGTTATATTATCTTCTGAGATCTGGCCTTAATATCCTTTAT790.11593607372947272No Hit
CAATCTGGGATAGTTGAACTTAAATATTTAAATTCAATTTTAGATTTTAAAA790.11593607372947272No Hit
CTTCTTGGGATAATGAAACTATCTACAATTCTTGTTTTAGCACATCTGTTAT790.11593607372947272No Hit
GTTCTAGGGAGTTCACTGTAACCTCCGCCTCCTGGGTTCAAGTGATTCTCAT790.11593607372947272No Hit
TGACGAGGGAAATATCTTTATTTTGCTATCTGAAAGTTTTTAACGCATCATT790.11593607372947272No Hit
CAACCTGGGGTATGTTCATAAAGAGGACTTAAGTCTCTTGTCTTTGTTAAGA790.11593607372947272No Hit
AGTGCTGGGTATTGGATGAAATCCCAAGGGAGATGGACGTAAGAAGAGGTTA780.11446852849239077No Hit
TCAGCTGGGAGGTGTGTTTATTTTCTTCCTCCATTTTCCAAAGTTACATGAC770.11300098325530886No Hit
TTATAAGGGATTGTGCACGTGTACCCTAAAACTGAAAGTATTAAAAAAAAGA770.11300098325530886No Hit
GCACTGGGGGGTTTTCTTTAAAAATCATTTTCGTATTATGCTTTAAGTGCCA770.11300098325530886No Hit
CCAAATGGGGGGGAAAAATAACTGTTTGACTTTCTGGAATATGTGATATAAG770.11300098325530886No Hit
TGCCGGGATCACGCCCTGGCACTCCAGCCTGGGTGACAGAGACTTTGTCTCA760.1115334380182269No Hit
CTTGGTGGGGAAAATGGAATTTTGATAAAAAATCCCATTTTAGATGCTTTAG760.1115334380182269No Hit
GCTATGGGGATTGATTCAGTGATGTTTTTTCAATTGACAAAATATTTTTTTA750.11006589278114498No Hit
CTTGGTGGGAGTTTGTTCATGGTACATTTGTCTCCAGCCCTAGACTCAGAAA740.10859834754406304No Hit
AGTTGTGGGCGATCTGTACGGGGGAGGTCCAGCTCCCTGGATAATTGCTCTT730.10713080230698112No Hit
CATGAGGGGCATCTATCTGAGTGAGTCTGGCAGGCTTTTTAGTTCCTGAGTT730.10713080230698112No Hit
TTATAAGGGACCATGGGTGTACCTGCTTAGGTTAAAAATCATACCAAGGCCT730.10713080230698112No Hit
GCCAACGGGATAATTTATATGTAAAAATAGGTAACTTTTATGATACTATTTT730.10713080230698112No Hit
GGCTCAGGGATCCTTGCATACCTGGAATAAACCCATTTGATCATGGTGTATT730.10713080230698112No Hit
TGATTAGGGGAATCAACTTTCCACTTAGTTTCCAATTTTCCCCTAGTCCACT730.10713080230698112No Hit
CTAATGGAATTCCTGCTGTACCATGCCATGCTCCCTCTCATTCTGAATCTCA720.10566325706989918No Hit
CCACTAGGGGAGCGGCAGAGGCAGCATCCAGCGGCGGCGCCAGCAGTTCCAG720.10566325706989918No Hit
TTATCTGGGAGAGATGAGCCACTGTGCTCAGCCCTTTCTGTAATATTTTATT720.10566325706989918No Hit
CTAAAGGGAATTTATTATAGAAATGATATTCTCACAATGATTTCATTTGTAG710.10419571183281724No Hit
CACAAAGGGATGGTTAAGGAATTTTCAGCACAAATTATTGCTTGGAGACCAT710.10419571183281724No Hit
CAGCCTGGGTGACAGAGCGAGACTTCGTCTCAAAAACAAACAAACAAACAAA710.10419571183281724No Hit
ATACTTGGGGACTATGTAATTACTAACCACCTGTCAGCTTCCATTTGCCCTT710.10419571183281724No Hit
GCAAAGGGGATTCTCCAAGGCATTATCACCAGCCAACATGATATATATACAG710.10419571183281724No Hit
GCATGCGGGACCTCTCCCAAGCCCCCATTGTGTGTTGAAATCCTGTCATGAA710.10419571183281724No Hit
GTACATGGGAGAAAATGAGTTGATTTTTAGAAATGTTTTTAATTGTAAAGAT700.10272816659573532No Hit
CGTACCGGGGCTTATAAGAACTTTTACAGGACTGTATTCATACACCAAATGT700.10272816659573532No Hit
TGGCTTGGGATAAGTGAAGTGAGGCTATTATTTTCTTTTTTGTACTTCCCTG700.10272816659573532No Hit
GTTCAGGGGAGTCTCTTCCCCACCAGGCAATGGAAGTCACCTCTGGCAATAT700.10272816659573532No Hit
TTTGTAGGGGCTTTCCTTTCAGCGGAGCGCGGCGGCAAGATGGCAGTGCAAA700.10272816659573532No Hit
ATAGGTGGGCTTGCTGGCTGATGCTGCTGTCTCTCAGTGGTTTATGATGAAG700.10272816659573532No Hit
CCTATTGGGCTACGCACGGGATTATTGAAGATCTTAAAAAACACGATTTTAT700.10272816659573532No Hit
TATATAGGGGAGCTTCCAAACCCAGGGCTTGCGCTTGCCTTTGCCTCTTCCA700.10272816659573532No Hit
CTAAGTGGGATTTCCTTCAGTGTCAAACATATGTAAGAGATGTTCCAAGCCC700.10272816659573532No Hit
CGGCAAGGGAACAGTACCTTTCTCAGTTTTTCTCTCTGAGATCACATGACGT690.10126062135865338No Hit
AAATCGGGGAATTGTGATAGATCAAGAAAGCAAAAAAACAAATACAGAAAGT690.10126062135865338No Hit
TACCGTGGGATAATAAATTAACCCATACAGATCTGAAGCCTGAAAATATTTT690.10126062135865338No Hit
CTATTTAAGGGCATGATGGCTCCAGTAGATTCTACCTCTTGGTCTTTAATTT690.10126062135865338No Hit
AACACAGGGATTAGAGAATCTATTCCAGGTGTGAGCCTCAGCAGCGATTTCA690.10126062135865338No Hit
GGTTTCGGGCTCCGTGTTCTACTATGAGATCCAGAATGCACCTGAGCAAGCC690.10126062135865338No Hit
ACTATTGGGTTTCTAGTCAGAGTCACTGTAATTCTTTTAAACCTTCAGATCT690.10126062135865338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)