FastQCFastQC Report
Sat 31 Dec 2022
SRR5844475.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844475.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11792
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGCCGTCT1421.2042062415196744No Hit
AAGCCAGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1231.0430800542740841No Hit
GCCTCAGGGGTAACTATAAAGAAAGGTCAGGTCACCTACAAAGGGAAGCCCA1010.8565128900949796No Hit
CGTCTAGGGCAAGAATGTGTGAGCAAAGATTTTCACATGAGGCTTCCCAGAC1000.8480325644504748No Hit
TGTCAAGGGAAAATCTTTAGTATGAAGTGAGAGAAAGAAGAGGGAGGCTGGT980.8310719131614654No Hit
TAACCTGGGGATTACAGTTATAATTTTGGAGTTTATTTTTCAAATCATGTTC970.8225915875169607No Hit
TGCAATGGGCATTCTCTGAGTCCCAGAGCAGATGTCCCCTCAGGTAGCAACC940.7971506105834464No Hit
TGGTATGGGAGAGGTTCAATTCCTCTTCTTAACAACATACCCATGGCCAACC930.7886702849389418No Hit
GAACTGGGGGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCAC920.7801899592944369No Hit
GTAATCGGGAAGCCCTAAAGCCAGTGTGGCTGTTATCTAGAGATGGACCTCT900.7632293080054274No Hit
GTTAGAGGGTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTT900.7632293080054274No Hit
CGCTGGGATTACAGGCGTGAGCCACCGCGCCCGGCTTCCAGAATATAAAAAA890.7547489823609227No Hit
AGATAAGGGAACCAAAGATTGTACTTCAGCAAGAATAGTAAACCAGGGAGAA890.7547489823609227No Hit
TTCTAGGGAGCCATGATCAAGGCGATCCTAATCTTCAACAACCACGGGAAGC880.7462686567164178No Hit
ACACGCGGGGATATATTTTTGTAATGTTGCCACCCATGTTGAAAACCTTAAA880.7462686567164178No Hit
TCAAGGGGGAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAAC880.7462686567164178No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGC860.7293080054274084No Hit
CAGCCAGGGGTGGTATTGCTACTCAGAAACCAGTACCACATGAAATCAGAAA790.6699457259158752No Hit
TATCAAGGGAGAGGGTCTTTGGATTCTCTCAGCGCTCCTGGTTTATTCCTGC770.6529850746268656No Hit
CGAGCCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC760.644504748982361No Hit
CTGGCGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC760.644504748982361No Hit
CGCTTGGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACA750.6360244233378561No Hit
AACAGGGGGATGTAAGAACATTATAAAGATAAAAGAAGAAATAAATGAAATT740.6275440976933514No Hit
GGTCAGGGGTAGCTTCGGTGCCATTTTTCTCTGTGCCCTTTTCCCTCCATGG720.6105834464043419No Hit
TTAGCAGGGATGACTCACAGCAGGTAGTTCTGTTGCACTGACTTGTTCATTA700.5936227951153324No Hit
GCACGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCA700.5936227951153324No Hit
GGCGTGGGGGACGACAGCGACAAGCCAGAGCGCGTTAGTGGCCCCTACGTGG680.5766621438263229No Hit
CATCAGGGTCTTCATGATTTAGTATCAGCTTATCTTAAAGAAAAAGAAAAAG670.5681818181818182No Hit
CGATCAGGGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTC640.5427408412483039No Hit
GGTCTCGGGCTGACAGAGAAATGAAATAGTTTTATCTGGAAAATTCCAGAGC640.5427408412483039No Hit
ATCCACGGGTACCTCTTCTATGAAGACATGAAGGAGAACCCCAAAAGGGAGA620.5257801899592944No Hit
TACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGG620.5257801899592944No Hit
GTCACTGGGAACTGCACCAAATTTCAACCTGACGGGTGTGGTGCTTATTTCC590.5003392130257802No Hit
ACCGCAGGGGCCTTGCATTATTGTTGGGCTATTTGCATGATTTCCTAAAATA590.5003392130257802No Hit
CTCCAAGGGACGCCATCAAGAGTGAGGTGGAGGCAGAGGAGCGGGGCGCCCG580.4918588873812755No Hit
TAAGCAGGGTTAACCACTAAGGATTATCAAGATTTTTGCGCAGACTTATACA570.4833785617367707No Hit
TTAATAGGGGCATGACAATGAGCGTGTGATCCATTACCAAGTCTCCTCATGA570.4833785617367707No Hit
CATTTGGGGCCAGCAACCAAAAGGTGAGCCAGAAGCAGGGCCTTAAATGAAC560.47489823609226595No Hit
TTTGACGGGTCCAGGGAAGAGGGGCTGGGGTTTAAATAGCATGCTTGGAGGA550.46641791044776115No Hit
TTTCTAGGGATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGC540.4579375848032564No Hit
GAATAAGGGGACTATTACAATCTCCATCTTCCCAGGCAATCTTGTGTCGTTA520.44097693351424694No Hit
GGGCTTAGGGATATATTATATTATATAAAACATATAATGTAATATATGATCT510.4324966078697422No Hit
GACAGTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA500.4240162822252374No Hit
TTCTTAGGGAGCCTACCCTTGGTGGTCTAAACGGATGCTGCTGGGTGTTGCG490.4155359565807327No Hit
TGTCGCGGGCAATTAATAAAATATTTTTGCCATAGTATGTGACTCTACATAA490.4155359565807327No Hit
AATCCAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG490.4155359565807327No Hit
TTCAACGGGGGGGCGTGGCTCAGGCTCGTGGAGGCGTCCGAGCTCCAGGGCA480.40705563093622793No Hit
TTTAAGGGGAAGAAACTTGTGGTGGAGAATGTTGATGTGTTAACACAAATGA470.3985753052917232No Hit
TCTGTCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC470.3985753052917232No Hit
GGCTATGGGGGCACTGGCCTTCAACCAAGCCAAGCGCCTTGGCTTCTGTCCT460.39009497964721845No Hit
GTAGGTGGGGAGAAATTTATAGCACTAAATGCCCACAAGAGAAAGCAGGAAA460.39009497964721845No Hit
ATAATGGGGCGAAAGAAAAGAGACTCATCACATACAGGGAACCCTTTTAAGG460.39009497964721845No Hit
TAACTAGGGGAAGTGGCCCTTGCAGGCAAGAGTGCTGGAGGGCGGCAGCGGC440.3731343283582089No Hit
ATAGTGGGGCAGGCTGCCATCAGAGGGGCCCCGGCCGGCCCACGTGGTAGTG430.3646540027137042No Hit
CTGCTTGGGGACCAGGAAAATGATGAAAAAGATGAAAAGGAGCAAGTTGCTA430.3646540027137042No Hit
TTCAGTGGGGTCCAAATTAAAGGAATGAGAATACTTAACTCAGTCTTCTTTT430.3646540027137042No Hit
GCTTTAGGGTGCTGTGGTGCATGAGATTCAGCGGTTCATCACCCTCGTGCCC410.3476933514246947No Hit
GGACCAGGGAAGATCTGTCTTGTAAACCACATTCTTGACAACTATTTGCTTT400.33921302578018997No Hit
CCTTGGCGGGAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGG400.33921302578018997No Hit
CTAAGTGGGGAGTCCTCGAAGGCTCGGAAGCCCGTCACCATGTCGTGCGAGT400.33921302578018997No Hit
GTAGTAGGGTTATCTAATTTTGGCCTGTGGTTTCTCTTCTGCTTAGGCATAA380.3222523744911805No Hit
TATTTAGGGGTGAAACAGGAGGTTGTTGATGTAATGAAAAAATCCTCAGAAG370.3137720488466757No Hit
GGCAATGGGTTGAGGAAAATTAATATCACAGCATAACCCCACCCTTTACATT370.3137720488466757No Hit
GGCTGAGGGTCTCATAGGAGCTATAAAGTATAATGCAATAATTGTTTCATTA360.30529172320217096No Hit
TTGTTAGGGGGGGCCTGGATGCAAGCATGTTGGGTATGCATTGTAGGGTAAT350.2968113975576662No Hit
ATTTGGGGGGGCTATGTGTTAAGGTTGCAGAGGCATTAAGATCTAAACTCTG350.2968113975576662No Hit
TTAGAAGGGGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGAT350.2968113975576662No Hit
AAGCTGGGGATTGTTTAAAAATTGTACAAAAGGAAAAAATTAGAATAAGTAC340.28833107191316143No Hit
AGCACTGGGAGTTGAGAGGGTGCAGTCCAGACAGCTTGACTGCTTTTAAATG310.2628900949796472No Hit
AATTCCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC310.2628900949796472No Hit
AGGATGGGGGACTGTTCCACCAACAACCCCTCCCAGGCTAAGCTGCGGCGGG310.2628900949796472No Hit
GTTTAAGGGAGGCTGTTCCTTGTACTCTTAGGATATTTGAGTCACTTGTGTC300.2544097693351425No Hit
TTACGAGGGTGACCCATAGCCCATAAGGCCTCAACTCTGGGACAGTGGGAAC300.2544097693351425No Hit
TGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACAC290.24592944369063774No Hit
TAAAGCGGGTAACGATGATGCTGTTTCACCAAGTATATTTTGAATTGGTTTC290.24592944369063774No Hit
TCTACAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA290.24592944369063774No Hit
TTGCTTGGGGGCTTCGGGACTGCGGAGACTACACACCGAGCGAGCGCCTGGG290.24592944369063774No Hit
CCAGCCTGGGTGACGGAGTGAGATCCTGTCTCAAAAAAAATAAAACAGATGT290.24592944369063774No Hit
AGATAAGGACTTTGCCATTGATATTATTAAAAGCACTCATGACCATTGGAAA280.23744911804613297No Hit
CACACTGGGATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTT270.2289687924016282No Hit
TCAGCGTGGGACCTTTATCAAGATGCTATTAAAATTGAACATCAAGTATAAT270.2289687924016282No Hit
TTATGCGGGGAGCAGATGTGGATCAGCAAGCAGGAGTATGACGAGTCCGGCC260.22048846675712347No Hit
AACTCCGGGGTTGCACAAATCTAACTGGTATTTTGTTTTTGTTTTTAGGAGG260.22048846675712347No Hit
GCATTCGGGGGAAGCGGAGCAAGGTCGAGGCCGGGTTGGCGCCGGAGCCGGG260.22048846675712347No Hit
GGTTTGGGGATAATCACTTACAATCTCTGTAAATAAGGAACTATTTATGAGG250.2120081411126187No Hit
AGTTTAGGGGGGATTACAGGCGTGAGCCATCGCGCCTGGCCATGATACAGTT250.2120081411126187No Hit
TACTCGGGAGTGGGAAGGCCTGGGATTTGCTGCTGCTCCCTTAGAGCAGGGC250.2120081411126187No Hit
AGTTGTGGGGGACCTACAGCCGCCCGGGAGAAGATGGCTGCCCCAGCAGTGT250.2120081411126187No Hit
ATTAATGGGGGCAGAGGCCAAGCCCAGACTCCAGACGGACATGCGGATAAGG240.20352781546811397No Hit
TAAAAAGGGTTATGAAAAAAGCTTTCTTTGCCTACTGCAGCTATCTAACATT240.20352781546811397No Hit
AGACCTGGGCAGGCCTTTTCAGTTAAGGGGAAGAGGTTGAAATGGGCTGAGA220.18656716417910446No Hit
TTTATCGGGACCTGACCTGCCGTCTAGAAAAACCTGCCAAATATGATGACAT210.17808683853459972No Hit
TGTCCTGGGCCTCATGTTTATGTTTTTTGATTTGTGTTTGTATATTTAAACA210.17808683853459972No Hit
TCGTGGGGGTTTGTCATGGGGGAACTGCCCTTTAAATTTTAAGTGACACTAC200.16960651289009498No Hit
AACTCTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC200.16960651289009498No Hit
GCCGCTGGGATAGTTGGGAGTAAAGCACTCCTCAGCAAATGTAAAAGAACAG190.16112618724559025No Hit
AAAACTGGGATCCCTGCCCCCACGTTCACCACTGACAACCCAACTCCATCCT190.16112618724559025No Hit
TTTCATGGGTTATGTACATAAATATGAAGAGGAAATTATCTTCAGGTTGATA190.16112618724559025No Hit
CACACGGGGATCTGGAGTTATCAGGGAGCTGCAAAAGGGTTGGGACTATCGA180.15264586160108548No Hit
GAGCAAGGGGAAATATTTTTTAATTTAAAGCTTACTCTGTCCATTTATCCAC180.15264586160108548No Hit
ATTAGTGGGACTGGGACGATTCCGCGGAGCCGGGCAGAGGTTTTAGGGGAAT180.15264586160108548No Hit
AGGTTAGGGTAGAATTAAAAGCAACTTGACTTTTGGGTTTTGCTTGGAGGTA180.15264586160108548No Hit
ATAGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG170.14416553595658071No Hit
GTATCGGGGATCCCCTTCCCTGCTTTATTTTTCTCCTCAGCATGTATTGTTA170.14416553595658071No Hit
GCGAAAGGGGCTCCACTGTCTGTGACCTTTTGCAGGGATTTTGTTCCTCTAA170.14416553595658071No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAA170.14416553595658071No Hit
CTTCTCGGGTCCTTTATTTACATTGGGTTTTTCTTTCGTAGTTTTGGTTTTT170.14416553595658071No Hit
CAATATCGGGATGGATGCAGACCCTGTTCTTGTTTCCCTCTGGAAGTCCTTA170.14416553595658071No Hit
AAATAGGGGCATTTTTCTAAGAGATGAGGGAAACCCTTTCTTTTGACTAAAT160.13568521031207598No Hit
GCGCTGGGGAAAGGTCAGAGATTGTAAATGGTCAATACTGACTTTTTTTTTA160.13568521031207598No Hit
GTGAGAGGGAGCATCATTGCTATTTATGACAATGACTTTAAGAAATGCAAGA160.13568521031207598No Hit
ATGCTGGGGCCCTGTGTCTACCTCTTTTATGGTACAGGCTGGTGGACTGGAA160.13568521031207598No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAAA160.13568521031207598No Hit
CTCCATCGGGGCATTGGGATAACAACTTTCATCACCAATCATTGGACTTTTG160.13568521031207598No Hit
ATAACCGGGGGGGTGAGGAGATGGGGGTCCAGTGATTTTCTTCTGATGTCTT150.12720488466757124No Hit
CGTTGCGGGCGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGAAACC150.12720488466757124No Hit
GCCCATGGGATTGTCATTCATCTCACTCTGGGCACCTGAGGTCCATAAGCGT150.12720488466757124No Hit
AGCAAAGGGACGGGCGTCCGCCGCCATGCCCGGCTAATTTTTGTATTTTTGG150.12720488466757124No Hit
TATTCCGGGGCTTCTCTGGTGTCATGGCTAAGTTCCATAGACATGAAGTACC150.12720488466757124No Hit
GCCAAAGGGGGCGTCTGCCAGGAGCTACGGCCGGAAGATGGCGGCGGCCGCA150.12720488466757124No Hit
TCCTCGTGGGGAGGGGCAGAAGCAGGGCCGGCAAAGGTTGTACCTTCATAAG150.12720488466757124No Hit
GACCAAGGGGGAAGTCCGTAGTGTCTCATTGCAGATAATTTTTAGCTTAGGG140.11872455902306649No Hit
TGACCTGGGGTTTGTTTAGACGAGATCATACCGGTAAAGCAGGAATGACAAA140.11872455902306649No Hit
CAATCAGGGTATGAAAATGCCATAGAGAAGTATGTTGATTTTAGCAGATCCT130.11024423337856173No Hit
GATCTAGGGTTGGGAGACAGAAGAGGCATCTGTGCACAGCTCGATCTTCTAC130.11024423337856173No Hit
CATGTAGGGACCAAAACAGAGATATAGATCAATGGAACAGAACAGAGCCCTC120.10176390773405698No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)