FastQCFastQC Report
Sat 31 Dec 2022
SRR5844474.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844474.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11792
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGCCGTCT1291.0939620081411128No Hit
GAACTGGGGGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCAC1060.8989145183175035No Hit
GTTAGAGGGTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTT1050.8904341926729986No Hit
AAGCCAGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1040.8819538670284939No Hit
CGTCTAGGGCAAGAATGTGTGAGCAAAGATTTTCACATGAGGCTTCCCAGAC1020.8649932157394844No Hit
CGCTGGGATTACAGGCGTGAGCCACCGCGCCCGGCTTCCAGAATATAAAAAA1000.8480325644504748No Hit
TAACCTGGGGATTACAGTTATAATTTTGGAGTTTATTTTTCAAATCATGTTC1000.8480325644504748No Hit
TGTCAAGGGAAAATCTTTAGTATGAAGTGAGAGAAAGAAGAGGGAGGCTGGT970.8225915875169607No Hit
GTAATCGGGAAGCCCTAAAGCCAGTGTGGCTGTTATCTAGAGATGGACCTCT960.8141112618724559No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGC950.8056309362279511No Hit
ACACGCGGGGATATATTTTTGTAATGTTGCCACCCATGTTGAAAACCTTAAA950.8056309362279511No Hit
TGGTATGGGAGAGGTTCAATTCCTCTTCTTAACAACATACCCATGGCCAACC950.8056309362279511No Hit
AGATAAGGGAACCAAAGATTGTACTTCAGCAAGAATAGTAAACCAGGGAGAA950.8056309362279511No Hit
TCAAGGGGGAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAAC910.7717096336499321No Hit
TGCAATGGGCATTCTCTGAGTCCCAGAGCAGATGTCCCCTCAGGTAGCAACC890.7547489823609227No Hit
CGCTTGGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACA820.6953867028493894No Hit
GCCTCAGGGGTAACTATAAAGAAAGGTCAGGTCACCTACAAAGGGAAGCCCA790.6699457259158752No Hit
GGCGTGGGGGACGACAGCGACAAGCCAGAGCGCGTTAGTGGCCCCTACGTGG750.6360244233378561No Hit
GGTCTCGGGCTGACAGAGAAATGAAATAGTTTTATCTGGAAAATTCCAGAGC750.6360244233378561No Hit
GGTCAGGGGTAGCTTCGGTGCCATTTTTCTCTGTGCCCTTTTCCCTCCATGG730.6190637720488467No Hit
TCTGTCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC700.5936227951153324No Hit
TTCTAGGGAGCCATGATCAAGGCGATCCTAATCTTCAACAACCACGGGAAGC690.5851424694708277No Hit
GCACGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCA690.5851424694708277No Hit
TACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGG690.5851424694708277No Hit
GTAGGTGGGGAGAAATTTATAGCACTAAATGCCCACAAGAGAAAGCAGGAAA680.5766621438263229No Hit
TTTCTAGGGATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGC650.5512211668928086No Hit
TTCTTAGGGAGCCTACCCTTGGTGGTCTAAACGGATGCTGCTGGGTGTTGCG650.5512211668928086No Hit
CGATCAGGGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTC650.5512211668928086No Hit
CTCCAAGGGACGCCATCAAGAGTGAGGTGGAGGCAGAGGAGCGGGGCGCCCG640.5427408412483039No Hit
AACAGGGGGATGTAAGAACATTATAAAGATAAAAGAAGAAATAAATGAAATT630.5342605156037992No Hit
CATCAGGGTCTTCATGATTTAGTATCAGCTTATCTTAAAGAAAAAGAAAAAG630.5342605156037992No Hit
TTAGCAGGGATGACTCACAGCAGGTAGTTCTGTTGCACTGACTTGTTCATTA610.5172998643147897No Hit
ACCGCAGGGGCCTTGCATTATTGTTGGGCTATTTGCATGATTTCCTAAAATA600.508819538670285No Hit
CGAGCCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC590.5003392130257802No Hit
GGGCTTAGGGATATATTATATTATATAAAACATATAATGTAATATATGATCT590.5003392130257802No Hit
CTGGCGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC590.5003392130257802No Hit
TTAATAGGGGCATGACAATGAGCGTGTGATCCATTACCAAGTCTCCTCATGA580.4918588873812755No Hit
CTAAGTGGGGAGTCCTCGAAGGCTCGGAAGCCCGTCACCATGTCGTGCGAGT570.4833785617367707No Hit
TATCAAGGGAGAGGGTCTTTGGATTCTCTCAGCGCTCCTGGTTTATTCCTGC540.4579375848032564No Hit
CAGCCAGGGGTGGTATTGCTACTCAGAAACCAGTACCACATGAAATCAGAAA520.44097693351424694No Hit
CTGCTTGGGGACCAGGAAAATGATGAAAAAGATGAAAAGGAGCAAGTTGCTA510.4324966078697422No Hit
GTCACTGGGAACTGCACCAAATTTCAACCTGACGGGTGTGGTGCTTATTTCC510.4324966078697422No Hit
TATTTAGGGGTGAAACAGGAGGTTGTTGATGTAATGAAAAAATCCTCAGAAG500.4240162822252374No Hit
AATCCAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG500.4240162822252374No Hit
ATTTGGGGGGGCTATGTGTTAAGGTTGCAGAGGCATTAAGATCTAAACTCTG500.4240162822252374No Hit
TAAGCAGGGTTAACCACTAAGGATTATCAAGATTTTTGCGCAGACTTATACA490.4155359565807327No Hit
TGTCGCGGGCAATTAATAAAATATTTTTGCCATAGTATGTGACTCTACATAA490.4155359565807327No Hit
ATCCACGGGTACCTCTTCTATGAAGACATGAAGGAGAACCCCAAAAGGGAGA490.4155359565807327No Hit
GAATAAGGGGACTATTACAATCTCCATCTTCCCAGGCAATCTTGTGTCGTTA490.4155359565807327No Hit
TAACTAGGGGAAGTGGCCCTTGCAGGCAAGAGTGCTGGAGGGCGGCAGCGGC470.3985753052917232No Hit
ATAATGGGGCGAAAGAAAAGAGACTCATCACATACAGGGAACCCTTTTAAGG450.3816146540027137No Hit
TTCAACGGGGGGGCGTGGCTCAGGCTCGTGGAGGCGTCCGAGCTCCAGGGCA450.3816146540027137No Hit
GGCAATGGGTTGAGGAAAATTAATATCACAGCATAACCCCACCCTTTACATT440.3731343283582089No Hit
TTTGACGGGTCCAGGGAAGAGGGGCTGGGGTTTAAATAGCATGCTTGGAGGA430.3646540027137042No Hit
TTTAAGGGGAAGAAACTTGTGGTGGAGAATGTTGATGTGTTAACACAAATGA420.35617367706919945No Hit
GGCTATGGGGGCACTGGCCTTCAACCAAGCCAAGCGCCTTGGCTTCTGTCCT420.35617367706919945No Hit
TTAGAAGGGGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGAT400.33921302578018997No Hit
TCTACAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA380.3222523744911805No Hit
GCTTTAGGGTGCTGTGGTGCATGAGATTCAGCGGTTCATCACCCTCGTGCCC370.3137720488466757No Hit
AGGATGGGGGACTGTTCCACCAACAACCCCTCCCAGGCTAAGCTGCGGCGGG370.3137720488466757No Hit
AGTTGTGGGGGACCTACAGCCGCCCGGGAGAAGATGGCTGCCCCAGCAGTGT370.3137720488466757No Hit
ATAGTGGGGCAGGCTGCCATCAGAGGGGCCCCGGCCGGCCCACGTGGTAGTG360.30529172320217096No Hit
CATTTGGGGCCAGCAACCAAAAGGTGAGCCAGAAGCAGGGCCTTAAATGAAC360.30529172320217096No Hit
TTGCTTGGGGGCTTCGGGACTGCGGAGACTACACACCGAGCGAGCGCCTGGG360.30529172320217096No Hit
GTAGTAGGGTTATCTAATTTTGGCCTGTGGTTTCTCTTCTGCTTAGGCATAA350.2968113975576662No Hit
CCTTGGCGGGAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGG350.2968113975576662No Hit
AATTCCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC350.2968113975576662No Hit
GGCTGAGGGTCTCATAGGAGCTATAAAGTATAATGCAATAATTGTTTCATTA340.28833107191316143No Hit
ATTAATGGGGGCAGAGGCCAAGCCCAGACTCCAGACGGACATGCGGATAAGG330.2798507462686567No Hit
TCAGCGTGGGACCTTTATCAAGATGCTATTAAAATTGAACATCAAGTATAAT330.2798507462686567No Hit
GACAGTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA330.2798507462686567No Hit
GGACCAGGGAAGATCTGTCTTGTAAACCACATTCTTGACAACTATTTGCTTT320.27137042062415195No Hit
AGACCTGGGCAGGCCTTTTCAGTTAAGGGGAAGAGGTTGAAATGGGCTGAGA310.2628900949796472No Hit
TAAAAAGGGTTATGAAAAAAGCTTTCTTTGCCTACTGCAGCTATCTAACATT310.2628900949796472No Hit
TTGTTAGGGGGGGCCTGGATGCAAGCATGTTGGGTATGCATTGTAGGGTAAT300.2544097693351425No Hit
CCAGCCTGGGTGACGGAGTGAGATCCTGTCTCAAAAAAAATAAAACAGATGT290.24592944369063774No Hit
TTATGCGGGGAGCAGATGTGGATCAGCAAGCAGGAGTATGACGAGTCCGGCC280.23744911804613297No Hit
TTCAGTGGGGTCCAAATTAAAGGAATGAGAATACTTAACTCAGTCTTCTTTT280.23744911804613297No Hit
TTACGAGGGTGACCCATAGCCCATAAGGCCTCAACTCTGGGACAGTGGGAAC280.23744911804613297No Hit
TAAAGCGGGTAACGATGATGCTGTTTCACCAAGTATATTTTGAATTGGTTTC270.2289687924016282No Hit
TGTCCTGGGCCTCATGTTTATGTTTTTTGATTTGTGTTTGTATATTTAAACA260.22048846675712347No Hit
ATTAGTGGGACTGGGACGATTCCGCGGAGCCGGGCAGAGGTTTTAGGGGAAT250.2120081411126187No Hit
GCCGCTGGGATAGTTGGGAGTAAAGCACTCCTCAGCAAATGTAAAAGAACAG240.20352781546811397No Hit
TCCTCGTGGGGAGGGGCAGAAGCAGGGCCGGCAAAGGTTGTACCTTCATAAG240.20352781546811397No Hit
AGTTTAGGGGGGATTACAGGCGTGAGCCATCGCGCCTGGCCATGATACAGTT230.19504748982360923No Hit
AGATAAGGACTTTGCCATTGATATTATTAAAAGCACTCATGACCATTGGAAA230.19504748982360923No Hit
AGCACTGGGAGTTGAGAGGGTGCAGTCCAGACAGCTTGACTGCTTTTAAATG220.18656716417910446No Hit
CACACTGGGATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTT220.18656716417910446No Hit
TCGTGGGGGTTTGTCATGGGGGAACTGCCCTTTAAATTTTAAGTGACACTAC210.17808683853459972No Hit
GCATTCGGGGGAAGCGGAGCAAGGTCGAGGCCGGGTTGGCGCCGGAGCCGGG210.17808683853459972No Hit
TATTCCGGGGCTTCTCTGGTGTCATGGCTAAGTTCCATAGACATGAAGTACC210.17808683853459972No Hit
AAAACTGGGATCCCTGCCCCCACGTTCACCACTGACAACCCAACTCCATCCT200.16960651289009498No Hit
GTTTAAGGGAGGCTGTTCCTTGTACTCTTAGGATATTTGAGTCACTTGTGTC190.16112618724559025No Hit
GGTTTGGGGATAATCACTTACAATCTCTGTAAATAAGGAACTATTTATGAGG180.15264586160108548No Hit
GCGCTGGGGAAAGGTCAGAGATTGTAAATGGTCAATACTGACTTTTTTTTTA180.15264586160108548No Hit
TGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACAC170.14416553595658071No Hit
AGGTTAGGGTAGAATTAAAAGCAACTTGACTTTTGGGTTTTGCTTGGAGGTA170.14416553595658071No Hit
AAGCTGGGGATTGTTTAAAAATTGTACAAAAGGAAAAAATTAGAATAAGTAC170.14416553595658071No Hit
CACACGGGGATCTGGAGTTATCAGGGAGCTGCAAAAGGGTTGGGACTATCGA160.13568521031207598No Hit
TACTCGGGAGTGGGAAGGCCTGGGATTTGCTGCTGCTCCCTTAGAGCAGGGC160.13568521031207598No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAA160.13568521031207598No Hit
GTGAGAGGGAGCATCATTGCTATTTATGACAATGACTTTAAGAAATGCAAGA150.12720488466757124No Hit
TCCAATGGGATGCGATAGGCTTAATTAAGCTGATGGTCTGTATCTCATGTTC150.12720488466757124No Hit
CAATCAGGGTATGAAAATGCCATAGAGAAGTATGTTGATTTTAGCAGATCCT150.12720488466757124No Hit
ATAGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG140.11872455902306649No Hit
GCCCATGGGATTGTCATTCATCTCACTCTGGGCACCTGAGGTCCATAAGCGT140.11872455902306649No Hit
GTATCTGGGCCCAGGTGAATTAAATTTTTCCAGGACACTAGCTAGTGTGCCT140.11872455902306649No Hit
TTTCATGGGTTATGTACATAAATATGAAGAGGAAATTATCTTCAGGTTGATA140.11872455902306649No Hit
GACCAAGGGGGAAGTCCGTAGTGTCTCATTGCAGATAATTTTTAGCTTAGGG140.11872455902306649No Hit
AACTCTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC140.11872455902306649No Hit
CCCTAACTGATTTCCATCCAAACCTACCTTATAGAGCACAATATTAAGTGTT130.11024423337856173No Hit
AGCAAAGGGACGGGCGTCCGCCGCCATGCCCGGCTAATTTTTGTATTTTTGG130.11024423337856173No Hit
TGGAAAGGGACGCTCTTCCAATCTCTTTCCGGGTCACCTTAAAAGTACAAAA120.10176390773405698No Hit
CGTTGCGGGCGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGAAACC120.10176390773405698No Hit
CTCCATCGGGGCATTGGGATAACAACTTTCATCACCAATCATTGGACTTTTG120.10176390773405698No Hit
CTTCTCGGGTCCTTTATTTACATTGGGTTTTTCTTTCGTAGTTTTGGTTTTT120.10176390773405698No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)