FastQCFastQC Report
Sat 31 Dec 2022
SRR5844473.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844473.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11406
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGCCGTCT1321.157285639137296No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGC1110.9731720147290899No Hit
AAGCCAGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1020.8942661756970015No Hit
CGTCTAGGGCAAGAATGTGTGAGCAAAGATTTTCACATGAGGCTTCCCAGAC950.8328949675609327No Hit
TCAAGGGGGAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAAC940.824127652112923No Hit
CGCTGGGATTACAGGCGTGAGCCACCGCGCCCGGCTTCCAGAATATAAAAAA920.8065930212169033No Hit
TAACCTGGGGATTACAGTTATAATTTTGGAGTTTATTTTTCAAATCATGTTC910.7978257057688936No Hit
GAACTGGGGGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCAC900.7890583903208838No Hit
TGGTATGGGAGAGGTTCAATTCCTCTTCTTAACAACATACCCATGGCCAACC890.780291074872874No Hit
TGTCAAGGGAAAATCTTTAGTATGAAGTGAGAGAAAGAAGAGGGAGGCTGGT870.7627564439768543No Hit
CATCAGGGTCTTCATGATTTAGTATCAGCTTATCTTAAAGAAAAAGAAAAAG850.7452218130808347No Hit
AGATAAGGGAACCAAAGATTGTACTTCAGCAAGAATAGTAAACCAGGGAGAA850.7452218130808347No Hit
GCCTCAGGGGTAACTATAAAGAAAGGTCAGGTCACCTACAAAGGGAAGCCCA830.727687182184815No Hit
GTTAGAGGGTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTT830.727687182184815No Hit
TATCAAGGGAGAGGGTCTTTGGATTCTCTCAGCGCTCCTGGTTTATTCCTGC780.6838506049447659No Hit
TGCAATGGGCATTCTCTGAGTCCCAGAGCAGATGTCCCCTCAGGTAGCAACC760.6663159740487463No Hit
CGATCAGGGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTTC740.6487813431527266No Hit
ACACGCGGGGATATATTTTTGTAATGTTGCCACCCATGTTGAAAACCTTAAA740.6487813431527266No Hit
TTCTAGGGAGCCATGATCAAGGCGATCCTAATCTTCAACAACCACGGGAAGC730.6400140277047168No Hit
GGGCTTAGGGATATATTATATTATATAAAACATATAATGTAATATATGATCT710.6224793968086972No Hit
GGTCTCGGGCTGACAGAGAAATGAAATAGTTTTATCTGGAAAATTCCAGAGC690.6049447659126775No Hit
GGTCAGGGGTAGCTTCGGTGCCATTTTTCTCTGTGCCCTTTTCCCTCCATGG680.5961774504646677No Hit
CTGGCGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC670.587410135016658No Hit
CGCTTGGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACA650.5698755041206383No Hit
GCACGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCA640.5611081886726285No Hit
GGCGTGGGGGACGACAGCGACAAGCCAGAGCGCGTTAGTGGCCCCTACGTGG640.5611081886726285No Hit
GTAATCGGGAAGCCCTAAAGCCAGTGTGGCTGTTATCTAGAGATGGACCTCT640.5611081886726285No Hit
ACCGCAGGGGCCTTGCATTATTGTTGGGCTATTTGCATGATTTCCTAAAATA630.5523408732246186No Hit
GTAGGTGGGGAGAAATTTATAGCACTAAATGCCCACAAGAGAAAGCAGGAAA620.5435735577766088No Hit
CGAGCCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC620.5435735577766088No Hit
TTTCTAGGGATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGC610.5348062423285991No Hit
AATCCAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG600.5260389268805892No Hit
AACAGGGGGATGTAAGAACATTATAAAGATAAAAGAAGAAATAAATGAAATT590.5172716114325794No Hit
GAATAAGGGGACTATTACAATCTCCATCTTCCCAGGCAATCTTGTGTCGTTA570.4997369805365597No Hit
TCTGTCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC560.4909696650885499No Hit
CTAAGTGGGGAGTCCTCGAAGGCTCGGAAGCCCGTCACCATGTCGTGCGAGT560.4909696650885499No Hit
TACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGG550.4822023496405401No Hit
CAGCCAGGGGTGGTATTGCTACTCAGAAACCAGTACCACATGAAATCAGAAA540.47343503419253025No Hit
CTCCAAGGGACGCCATCAAGAGTGAGGTGGAGGCAGAGGAGCGGGGCGCCCG530.46466771874452045No Hit
ATAATGGGGCGAAAGAAAAGAGACTCATCACATACAGGGAACCCTTTTAAGG520.45590040329651055No Hit
TTCAACGGGGGGGCGTGGCTCAGGCTCGTGGAGGCGTCCGAGCTCCAGGGCA500.43836577240049096No Hit
TTAATAGGGGCATGACAATGAGCGTGTGATCCATTACCAAGTCTCCTCATGA490.4295984569524811No Hit
CCAGCCTGGGTGACGGAGTGAGATCCTGTCTCAAAAAAAATAAAACAGATGT480.4208311415044713No Hit
TAACTAGGGGAAGTGGCCCTTGCAGGCAAGAGTGCTGGAGGGCGGCAGCGGC450.3945291951604419No Hit
TAAGCAGGGTTAACCACTAAGGATTATCAAGATTTTTGCGCAGACTTATACA450.3945291951604419No Hit
TTCTTAGGGAGCCTACCCTTGGTGGTCTAAACGGATGCTGCTGGGTGTTGCG450.3945291951604419No Hit
ATCCACGGGTACCTCTTCTATGAAGACATGAAGGAGAACCCCAAAAGGGAGA450.3945291951604419No Hit
TATTCCGGGGCTTCTCTGGTGTCATGGCTAAGTTCCATAGACATGAAGTACC450.3945291951604419No Hit
GTCACTGGGAACTGCACCAAATTTCAACCTGACGGGTGTGGTGCTTATTTCC450.3945291951604419No Hit
GACAGTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA440.3857618797124321No Hit
ATAGTGGGGCAGGCTGCCATCAGAGGGGCCCCGGCCGGCCCACGTGGTAGTG420.3682272488164124No Hit
TTAGCAGGGATGACTCACAGCAGGTAGTTCTGTTGCACTGACTTGTTCATTA420.3682272488164124No Hit
ATTTGGGGGGGCTATGTGTTAAGGTTGCAGAGGCATTAAGATCTAAACTCTG400.3506926179203928No Hit
CTGCTTGGGGACCAGGAAAATGATGAAAAAGATGAAAAGGAGCAAGTTGCTA390.34192530247238295No Hit
TTACGAGGGTGACCCATAGCCCATAAGGCCTCAACTCTGGGACAGTGGGAAC390.34192530247238295No Hit
AATTCCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC390.34192530247238295No Hit
TCAGCGTGGGACCTTTATCAAGATGCTATTAAAATTGAACATCAAGTATAAT380.33315798702437316No Hit
AGGATGGGGGACTGTTCCACCAACAACCCCTCCCAGGCTAAGCTGCGGCGGG380.33315798702437316No Hit
TTTAAGGGGAAGAAACTTGTGGTGGAGAATGTTGATGTGTTAACACAAATGA370.3243906715763633No Hit
GTAGTAGGGTTATCTAATTTTGGCCTGTGGTTTCTCTTCTGCTTAGGCATAA370.3243906715763633No Hit
TATTTAGGGGTGAAACAGGAGGTTGTTGATGTAATGAAAAAATCCTCAGAAG360.31562335612835346No Hit
GGCTATGGGGGCACTGGCCTTCAACCAAGCCAAGCGCCTTGGCTTCTGTCCT360.31562335612835346No Hit
TTTGACGGGTCCAGGGAAGAGGGGCTGGGGTTTAAATAGCATGCTTGGAGGA360.31562335612835346No Hit
TTAGAAGGGGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGAT350.3068560406803437No Hit
TGTCGCGGGCAATTAATAAAATATTTTTGCCATAGTATGTGACTCTACATAA340.2980887252323339No Hit
GGCAATGGGTTGAGGAAAATTAATATCACAGCATAACCCCACCCTTTACATT340.2980887252323339No Hit
ATTAGTGGGACTGGGACGATTCCGCGGAGCCGGGCAGAGGTTTTAGGGGAAT330.289321409784324No Hit
GGCTGAGGGTCTCATAGGAGCTATAAAGTATAATGCAATAATTGTTTCATTA320.28055409433631423No Hit
TTCAGTGGGGTCCAAATTAAAGGAATGAGAATACTTAACTCAGTCTTCTTTT310.2717867788883044No Hit
ATTAATGGGGGCAGAGGCCAAGCCCAGACTCCAGACGGACATGCGGATAAGG310.2717867788883044No Hit
AGATAAGGACTTTGCCATTGATATTATTAAAAGCACTCATGACCATTGGAAA310.2717867788883044No Hit
AGCACTGGGAGTTGAGAGGGTGCAGTCCAGACAGCTTGACTGCTTTTAAATG300.2630194634402946No Hit
GGACCAGGGAAGATCTGTCTTGTAAACCACATTCTTGACAACTATTTGCTTT290.2542521479922848No Hit
GTGAGAGGGAGCATCATTGCTATTTATGACAATGACTTTAAGAAATGCAAGA290.2542521479922848No Hit
AGTTTAGGGGGGATTACAGGCGTGAGCCATCGCGCCTGGCCATGATACAGTT290.2542521479922848No Hit
CATTTGGGGCCAGCAACCAAAAGGTGAGCCAGAAGCAGGGCCTTAAATGAAC280.24548483254427494No Hit
GCCCATGGGATTGTCATTCATCTCACTCTGGGCACCTGAGGTCCATAAGCGT280.24548483254427494No Hit
TTGTTAGGGGGGGCCTGGATGCAAGCATGTTGGGTATGCATTGTAGGGTAAT270.23671751709626512No Hit
AGACCTGGGCAGGCCTTTTCAGTTAAGGGGAAGAGGTTGAAATGGGCTGAGA260.22795020164825527No Hit
AAAACTGGGATCCCTGCCCCCACGTTCACCACTGACAACCCAACTCCATCCT260.22795020164825527No Hit
AGTTGTGGGGGACCTACAGCCGCCCGGGAGAAGATGGCTGCCCCAGCAGTGT260.22795020164825527No Hit
GGTTTGGGGATAATCACTTACAATCTCTGTAAATAAGGAACTATTTATGAGG250.21918288620024548No Hit
CCTTGGCGGGAGTGCTCTGAGCCCTAGGATTCATCTTTCTTTTCACCGTAGG240.21041557075223566No Hit
TTGCTTGGGGGCTTCGGGACTGCGGAGACTACACACCGAGCGAGCGCCTGGG240.21041557075223566No Hit
AAGCTGGGGATTGTTTAAAAATTGTACAAAAGGAAAAAATTAGAATAAGTAC240.21041557075223566No Hit
GTTTAAGGGAGGCTGTTCCTTGTACTCTTAGGATATTTGAGTCACTTGTGTC230.20164825530422584No Hit
TGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACAC230.20164825530422584No Hit
TCTACAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA230.20164825530422584No Hit
TTATGCGGGGAGCAGATGTGGATCAGCAAGCAGGAGTATGACGAGTCCGGCC220.19288093985621604No Hit
TTTCATGGGTTATGTACATAAATATGAAGAGGAAATTATCTTCAGGTTGATA220.19288093985621604No Hit
TAAAAAGGGTTATGAAAAAAGCTTTCTTTGCCTACTGCAGCTATCTAACATT220.19288093985621604No Hit
CACACTGGGATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTT210.1841136244082062No Hit
GCTTTAGGGTGCTGTGGTGCATGAGATTCAGCGGTTCATCACCCTCGTGCCC210.1841136244082062No Hit
GCATTCGGGGGAAGCGGAGCAAGGTCGAGGCCGGGTTGGCGCCGGAGCCGGG210.1841136244082062No Hit
GCGCTGGGGAAAGGTCAGAGATTGTAAATGGTCAATACTGACTTTTTTTTTA200.1753463089601964No Hit
ATAACCGGGGGGGTGAGGAGATGGGGGTCCAGTGATTTTCTTCTGATGTCTT190.16657899351218658No Hit
AACTCCGGGGTTGCACAAATCTAACTGGTATTTTGTTTTTGTTTTTAGGAGG190.16657899351218658No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAA190.16657899351218658No Hit
CGTTGCGGGCGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGAAACC180.15781167806417673No Hit
GACCAAGGGGGAAGTCCGTAGTGTCTCATTGCAGATAATTTTTAGCTTAGGG180.15781167806417673No Hit
TAAAGCGGGTAACGATGATGCTGTTTCACCAAGTATATTTTGAATTGGTTTC180.15781167806417673No Hit
AGGTTAGGGTAGAATTAAAAGCAACTTGACTTTTGGGTTTTGCTTGGAGGTA180.15781167806417673No Hit
TCGTGGGGGTTTGTCATGGGGGAACTGCCCTTTAAATTTTAAGTGACACTAC170.14904436261616694No Hit
TTTATCGGGACCTGACCTGCCGTCTAGAAAAACCTGCCAAATATGATGACAT160.14027704716815712No Hit
AGCAAAGGGACGGGCGTCCGCCGCCATGCCCGGCTAATTTTTGTATTTTTGG160.14027704716815712No Hit
CTCCATCGGGGCATTGGGATAACAACTTTCATCACCAATCATTGGACTTTTG160.14027704716815712No Hit
ATCAAAGGGTATTGCATGGGCTGCATTATCTACAGCATTGTACAATAACAAC150.1315097317201473No Hit
TCCAATGGGATGCGATAGGCTTAATTAAGCTGATGGTCTGTATCTCATGTTC150.1315097317201473No Hit
CATGTAGGGACCAAAACAGAGATATAGATCAATGGAACAGAACAGAGCCCTC150.1315097317201473No Hit
ATAGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG140.12274241627213747No Hit
AAATAGGGGCATTTTTCTAAGAGATGAGGGAAACCCTTTCTTTTGACTAAAT140.12274241627213747No Hit
TGTCCTGGGCCTCATGTTTATGTTTTTTGATTTGTGTTTGTATATTTAAACA140.12274241627213747No Hit
GCGAAAGGGGCTCCACTGTCTGTGACCTTTTGCAGGGATTTTGTTCCTCTAA140.12274241627213747No Hit
AACTCTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC140.12274241627213747No Hit
GTATAGAGGGGGTGTGAACCCATGTCCAGGCACGCACCGACACACATGCACG140.12274241627213747No Hit
CTTCTCGGGTCCTTTATTTACATTGGGTTTTTCTTTCGTAGTTTTGGTTTTT140.12274241627213747No Hit
TACTCGGGAGTGGGAAGGCCTGGGATTTGCTGCTGCTCCCTTAGAGCAGGGC130.11397510082412764No Hit
CACATAGGGCACTTCTGTGTTATAATGGAAGGTTTTTTGTGATGTATGAAAC130.11397510082412764No Hit
TGCTCAGGGAATCTCAAGACACCTCCCGAGTGTCTGCTCACTCCCCTTCCAC130.11397510082412764No Hit
AAAGTTGGGACTAGACATCGTACTACACGACACGTACTACGTTGTAGCCCAC120.10520778537611783No Hit
GAGCAAGGGGAAATATTTTTTAATTTAAAGCTTACTCTGTCCATTTATCCAC120.10520778537611783No Hit
TGGAAAGGGACGCTCTTCCAATCTCTTTCCGGGTCACCTTAAAAGTACAAAA120.10520778537611783No Hit
GTATCTGGGCCCAGGTGAATTAAATTTTTCCAGGACACTAGCTAGTGTGCCT120.10520778537611783No Hit
AGGATAGGGTATGACTAATTAATAATATTTGTTGTTATTTATTGTACAGATG120.10520778537611783No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAAA120.10520778537611783No Hit
TGACCTGGGGTTTGTTTAGACGAGATCATACCGGTAAAGCAGGAATGACAAA120.10520778537611783No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)