FastQCFastQC Report
Sat 31 Dec 2022
SRR5844471.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844471.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12375
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGCCGTCT1271.0262626262626262No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGC1050.8484848484848486No Hit
TCCTAAGGGCAAGAGATGGCTGCTGTACACTTTTTGCAACTGGTTTGATGTC380.30707070707070705No Hit
CGTCCTGGGGCAGCCAGGAATAGCCAAGTTTTTTCTAAAACTCTTACGGGAA340.27474747474747474No Hit
TTTCAAGGGATGAAGATGAGGCTGAAAGGGTTCTTTTTTCCTACGGCTATGG320.2585858585858586No Hit
AATAGGGGTAGTGAAGGGACTGTGTTGCTTGTGAAGCGGTGTAGTAGCATTT310.2505050505050505No Hit
AAAATGGGGATACTTCTCAAATAGCTATTGAAAGAATGTGTTCATTTTATTA310.2505050505050505No Hit
TGTGCAGGGATGTAAAATCCATTGTCGCATCCACCTTTATTATCAGTCTCTT300.24242424242424243No Hit
TTTTGAGGGATGCAGAGCGGAGGCTTCGCGCAGCAGAGCCCGCGCGCCGCCC280.22626262626262625No Hit
CTTAGTGGGAAACCAGTCAGGGCCGTCATGACTACGCAGCAGCAGCAGTTTC270.2181818181818182No Hit
GATCATGGGAATGAACACACGCCAACAGATAGCTTAGGTTTATTTGGTGACT270.2181818181818182No Hit
TGCTAGGGGCAGATTACATGTTTTTACAGTGGCCTGCTATTGAGGAAAGGTA260.2101010101010101No Hit
GCATTAGGGCGCGATGGTGTTGTTGGAGAGCGAGCAGTTCCTGACGGAGCTG260.2101010101010101No Hit
GTATTAGGAGAAGAAACATGGCAAACTGCTCTGTGCTTTCAAACCAAAGTGT260.2101010101010101No Hit
TTACAAGGGAACAGGAAGGAGAAGGAGTGGCCATTATGATGGGAGGACCAGC250.20202020202020202No Hit
GCTGCGGGGGCACCAAATCAAAATCTCTCCACTTTCAGCTGTCTTTTGGAGG250.20202020202020202No Hit
CCCTCCCGCTCCCCGTCCAAGGTGGAGGTGACCGAGAAGACGACAACTGTGC250.20202020202020202No Hit
TATTTGGGGGACAAGTTCCTGATCAAGAACTGCAATGATTTCCAGTATGAGA250.20202020202020202No Hit
ATTTACGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG250.20202020202020202No Hit
GTGCAAGGGTATGTCCCTTCACATAGTTTTTAAGGTTATTTATTTAAATGTC250.20202020202020202No Hit
TGGTCCGGGAAGTTTCTCAGCTCTTTTAACATGTCTTTCTTAGCATTTGAGC240.19393939393939394No Hit
ATTATCGGGTGATTAGAAAGGTGATGGAATATGTGACAATGCAAAATGAATT240.19393939393939394No Hit
AGGTCCCAGACTGATTACATGAGACTGGGGAGTCCTTCCCTGCCAGTTTTCT240.19393939393939394No Hit
GCACATGGGTTTGGGAAACTTTTAGGGAAATGAAAAATAAAAGTCACTGAGT240.19393939393939394No Hit
ACGCATGGGAGGGAGAGAGGGATGGTTTCTGAGGGGTTCTGAAAGTATGATT240.19393939393939394No Hit
TAGCAAGGGACAGCCTCAGCTCCAGGCTATCTGGCAATAACAAAGAAAGTGG240.19393939393939394No Hit
CGCAGAGGGAGTTCATACATATGCACCCAAAAACCAAAATAAGTCTCACTAA240.19393939393939394No Hit
TTAAATGGGGCTGTCGTGGGGGATTGTGGGAAAAGATGGCGGCTGCCGCACA230.18585858585858586No Hit
GCGCTGGGCTTTAAGCACTAATCCTATTCTAGGCCTTGCTGGGATGGACGTG230.18585858585858586No Hit
AAAAAAGGGACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT230.18585858585858586No Hit
TATATAGGGGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCT230.18585858585858586No Hit
CGGTCGGGATCATATTAGAAATTAAAACTTGTAATAAAAATATACTAATTTA220.17777777777777778No Hit
CTAATAAGGGACTGCTATGACGGTTAAGAATGTCAGTATACAAGAAGGAATA220.17777777777777778No Hit
AGTAGTGGGAGATTGTTAAAAGTTATGTTTGAATGTGTTAATTATTCAAGTA210.1696969696969697No Hit
TACGGCGGGCGATCTAGGATAGGGATCTTAGAGCGGGTTTCCACTGTGGCAA210.1696969696969697No Hit
TGAGATGGGAAGTTTAGTATATAGAGACAATTTTAATGGAAATAACTACTGT210.1696969696969697No Hit
AACGCAGGGAAGATGCGTATATGTACAATTTGGTGCCATTATTTCTCCTACG210.1696969696969697No Hit
GATAATGGGGTACTTGGGTGTTTCCCTCCATCCTTTCTGATATGACCAAAAA210.1696969696969697No Hit
TCTACGGGGAGAGCCTTCTTGCTTTCATTTTCCTTGAATGTACATAGGAACA210.1696969696969697No Hit
TACTTAGGGAAGTCGCTGACAGTCTACTAAGTTACACGGAACAGTATTTAGA210.1696969696969697No Hit
GTCTAAGGGAGATTTGCTTTATACAAGATTGTTGCAGTACCTTTTTCTGGTA210.1696969696969697No Hit
TGAACTGGGCCAGAAATTTATCTTCCTCATGTGCAGTTTAACAAACTTGGTC210.1696969696969697No Hit
AGCTGGGGGTTGGCCAGGGTGGTCTCGAACTCCTGACCTCAAGTGATCCGTC210.1696969696969697No Hit
AAACTTGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC210.1696969696969697No Hit
TTTCTAGGGATGTGATTAGCGCAGAGACCTTCACTGCCCTATCAGTACTAGG210.1696969696969697No Hit
CAATATGGGTATGATCTGGTAACTAGTTTCATGGAGACTAACATGGGAATCA200.1616161616161616No Hit
AGACCAGGGGAGTCCACGTTACGGATCGGCTTACTCCGCGGAGTTGGCCTCA200.1616161616161616No Hit
CAGCAGGGACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTAT200.1616161616161616No Hit
TGCACTGGGATGTTTAATAATAAATTGTAACTACATAACATAGATGGAGACT200.1616161616161616No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCC200.1616161616161616No Hit
ATTTCAGGGTAGAGGGGAGCTGGGAATTATGGTCTAGCTAGACCATGTGACC200.1616161616161616No Hit
TCTCTTGGGATTGCGGGGCCGCTTCCTTTCCGCTCGGCTGTTTTCCTGCGCA200.1616161616161616No Hit
GTATAGGGGAGCTTTCTCAGGAGAAAGGACGGTTGCACCAGCTGTATGAAAC190.15353535353535352No Hit
TAATGTGGGTCAATTTAATCGTTTACCTTTGGTGGTGAGTTTCATAGCCAGC190.15353535353535352No Hit
TAGACAGGGCATTCTTCTTGGCTCACCAACCCTGCAGGCCTCATCTTTCCCA190.15353535353535352No Hit
AGAACAGGGTGTCCAGTTAAAAATTGCTAAAAGCTGTAAATGTGCCTGCCTG190.15353535353535352No Hit
CTAGGGAGGGGCACAGTGAAGAAGGGAGCCCACCACCTCTCCGAAGAGGAAA190.15353535353535352No Hit
CCCAGCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA190.15353535353535352No Hit
GTCTATGGGAATGAAGAGATATTCAGCATTTGTCTATTTGATAAGGAATTAA190.15353535353535352No Hit
ACGTATGGGAAGGGGTAGAACCTATCTTGCCTTCACTCTCAAGATAACGACT190.15353535353535352No Hit
CGAGTGGGGAAAGTAAGAAAATCTTATTTATGATATATGCAGTTGACTTATC190.15353535353535352No Hit
GGTGCTGGGCCGGGGGCAGCAAACTGACATGGTTGAATGATAGCATTTCACT190.15353535353535352No Hit
GAACTTGGGAACATTACCTAAAAGGCACTAACATGCTCAGGTTCCCCAGAAA190.15353535353535352No Hit
TCGCGTGGGCCGATCTTATGAGTGGGCTCCCTAAAGCCCATGTCGAAGCCCC190.15353535353535352No Hit
GGTCGCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC190.15353535353535352No Hit
AGCCTGGGGCAAGACCGAGCCTCATCTCCTCCACGAAGCCTCCCTACTCCAT180.14545454545454545No Hit
TACTGTGGGGCAGGCCACTCTGTATCAGGCTCTTTGCTTATGCCAGGGCATG180.14545454545454545No Hit
TGTATGGGGAGATTTGCATCATTCAAGCATATCTTGTAAAACAAACACATAT180.14545454545454545No Hit
GAGTAAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC180.14545454545454545No Hit
AAGGTTGGGTCCCGAAAAACAATCTTCCAGTCTATAAATTGTTTGCTAGTAC180.14545454545454545No Hit
TACTTTGGGCTGGATTTCCCAGAGAGTCGGGTAATCAGTCAGCCAGATCAAA180.14545454545454545No Hit
AGCTCTGGGACACTGTGTGTAGAATTGGTTGTGTGGTGAAATACAATACTGT180.14545454545454545No Hit
AAGCTTGGGAGTTCGTGGAATTGAACAGAACCTTGATGGAATGCAGTTGCCT180.14545454545454545No Hit
TTTTTAGGGGATCTTTATCAGGGTAAGATAATCAATTATTTTTCCAAATTCT180.14545454545454545No Hit
TGGTTTAGGGACAACTCACAGATATGAATGAACAAGAGGAGGTATTACTAGA180.14545454545454545No Hit
ATTTGGGGGGGGTTGGGTCAGTGCCGCGCGCCGATCGGTCGTTACCGCGAGG180.14545454545454545No Hit
GTATTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC180.14545454545454545No Hit
CAAGGTGGGATCCTTTTGTCATAATTCAGATGCATTGACTTCTGCCACATTT180.14545454545454545No Hit
GTTCATGGGAGGATTCTTCGTGACATTGAGACTTTCTACAATACTACAGTGG170.13737373737373737No Hit
CAAGGGATAATGGATGTAGTCCCGAGGGGGCAGTTGGGGGAAGACAGGAGAT170.13737373737373737No Hit
CTGTAGGGAGGTTGCCTGTTCACTCTGATGGTAGTTTCTTTTGCTGTGCAGA170.13737373737373737No Hit
AGCTCTGGGGTCATGAAAAATGTTAGGATAGACACAGAAAGAGGGGAAAGGA170.13737373737373737No Hit
AGGCTGGGGGATAATAGGAGCCCCATAGGTAATTTAAAGTTTTCTGTTATTT170.13737373737373737No Hit
ATTAAGGGGGCTATTATTAGTCGACAAATAATTATAACACCCATTATGAGTC170.13737373737373737No Hit
CATTGACGGGACAGGGAAAATCTTCAAGAAAGAAAAGGAAATGAAGAAAGGT170.13737373737373737No Hit
ACAGGAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA170.13737373737373737No Hit
TCTAACGGGAAGAGAAAACAAAATGAAAATGTTGCCATGATTCCTTTTCACT170.13737373737373737No Hit
GTTAACGGGATCCCCAGCTGTCTTCAGTTCACCAAAAAACTCAAGAAGGCTC170.13737373737373737No Hit
TAAGCAGGGGAAGTCTTCTGCTAAAGAATTACCTCCAAAGCCATTGTCAAGA170.13737373737373737No Hit
TATCTAGGGTACTTTAATATGATTAGTTTACTGATAAATTAACCAGTAAAGA170.13737373737373737No Hit
TCAGATAGATTGAAAAAAAGGAAAGAATGTCACTTTGAAAACTATTAAGAAG170.13737373737373737No Hit
CATCCAGGGATGGAAACACCCTAAGTGTCCATCAATGGATGAATACATAAGG170.13737373737373737No Hit
CTCGGTTAGGGACCGGGTATTTAGGTGCTGGGAAGACAACACTTCTGAACTA170.13737373737373737No Hit
ACAGTCCGGGACCTTGTATAACTAACAAGCACAGTTATTGTTTAACATTATG170.13737373737373737No Hit
AAAAGGGGCAAGGGAATCTTGATCTGACTGGTTGTCAGGGGTTTTTTTCTAG170.13737373737373737No Hit
AAACATGGGATATACTGAAGCTTGAGTGGAAGAAAGGTTAAATCATTGATGC170.13737373737373737No Hit
AGTTCAGGGCAAATCAACAACAACCTATTTAGCTGTTCCCCAACCTTTTCCT160.1292929292929293No Hit
AGTCAGGGGATGAAGCTGGAGGGGTACACTTCTTGTGAGATCATGATTCCAT160.1292929292929293No Hit
CGACGAGGGGGGATTGCTTAATCCAACTCTGGAGAGGGACCAAGTCTTTTCT160.1292929292929293No Hit
TGCCATGGGGCATTCTAATCTCTCTGCCGCTTCAACTATGTGACCTGGGGCA160.1292929292929293No Hit
AAGAGGGGGACCAGAAAGAAAATATGGTTCAATGTACTTACAATGGAATCCC160.1292929292929293No Hit
GACCTGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC160.1292929292929293No Hit
TTGTATGGGAAGAGTCCGGATTTCTAACAGTCCTTGCTTTGGGGGGTGTGCT160.1292929292929293No Hit
TAATATGGGAGATGTGTTGATGCACACATGACTATTCTGTTTTTCTCACTGA160.1292929292929293No Hit
TCACTGGGGAAGTGCACTGTGTTCTCTCAAAGGAGATTTGCGTTGCTTTCTA160.1292929292929293No Hit
AGCGTAGGGTCTCCTCCACCTGGACATACTACTTGCAGACTGACAATGGGAA160.1292929292929293No Hit
CTGTCGGGTCCGATCTTATGTGGGGCATAAATACTGTAATACAGTGGAGAGA160.1292929292929293No Hit
TTATAGGGGGGGGTTCCCGGCGCGGGGAATGTCCCGGGTGGAGCTGGCTGAG160.1292929292929293No Hit
ATTCTCGGGGATAGAAGATGTTAATTTTGGGATGATAGATAGCTGATTGATT160.1292929292929293No Hit
GGACAAGGGTCCCCTGTAAGCCAAAAAGTATCTGAGACAAATACTTCCTGTT160.1292929292929293No Hit
TGCACTGGGAGATAGTCAGGGCCTGTAAAGTGTGCCAAAGAAATAATCCCCT160.1292929292929293No Hit
TAATAGGGGATGTCAGATAATGGCCAAAATGAGGAAAACCACGATGATAAAA160.1292929292929293No Hit
GTACTTGGGTAGCAAAGTGGATTGCTGACAGAACGGATGTTCATATTCGGGT160.1292929292929293No Hit
CTGTCTGGGTTATGGTGGAAGAGCCCCATAGGAAGACTGTTTTGAGTGGCCA160.1292929292929293No Hit
GGTTGGGACACACTTTGAATTCAAAATGGAAAACCCATTCCCTGGAATTAGA160.1292929292929293No Hit
AATAGAGGGTAATATATGCAGATGTAATGTTGACTATAAAGTATTAGCTCCT150.12121212121212122No Hit
AGAACGGGGGGTTGTTCACTTGCTGAGGTGCAAAAATTCTTAAGACTTCTGT150.12121212121212122No Hit
ACAACAGGGAGTTTGTTGAATATGATAAAAACAGTGATGATACTGTGACTTG150.12121212121212122No Hit
ATGCACGGGAACCGAGATAACTACTAAGTGACTACCAGGTGGGGAGTCTATA150.12121212121212122No Hit
ACTTGAGGGCAAAGCATTGACTGTTGTGTCATTAGCTGCCTGGTTACATTAG150.12121212121212122No Hit
GGTGCCGGGAACAGGACCTTACCTCACAGGACAGTGGCCACGGGATCCTCAT150.12121212121212122No Hit
GGGTCGGGATGACTTAGGTATTTCCCCCCAAACTTTAATATTCTTGAGCACT150.12121212121212122No Hit
ACATATGGGAGTTGGCTAGATATCGAGACTCATCCTCTGACTGAGAAAAACC150.12121212121212122No Hit
CGTCAAGGGGGTGAATAAGCACAGTGAAACCTTCACTCGCGATAACAACATG150.12121212121212122No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAA150.12121212121212122No Hit
CATATCGGGAATTCAGAGTGGTAGCCTGGAAGTGGTGAGAAGTAGTTGGATT150.12121212121212122No Hit
TACAAGGGGCCCCTGCTCATCTATCTTGAGGAGTTACGGTTCTTTTCCACTA150.12121212121212122No Hit
ATTGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC150.12121212121212122No Hit
AGACCAGGGGCACCTGGCCCAGGAATTTCCAATTTCCTTTTCTGCTCCAGAG150.12121212121212122No Hit
AGCCTTGGGCAACTATCAGTGATCTGCAACGTGGTAGATTGTGCTGATATCA150.12121212121212122No Hit
TTAGCTGGGCTCCCCACAAAAGGAAAAAAAAGCTGGATTTGATTTTTTTTTT150.12121212121212122No Hit
TCAAAAGGGAGTGCTCAAGAACAATTTGTGTCCCTCGGGCAGCAACATCATC150.12121212121212122No Hit
TTTGCTGGGTAGCATCTTCAGGTCTTTAGATCTTGGACAAGTTGGCAGGGTA140.11313131313131312No Hit
GTTTTGGGGTTCCGGGGATGACCCACCAATCACATGCCTATCATATAGTAAA140.11313131313131312No Hit
ATTCATGGGTAGTAAGAGATTTATAAAACAGAAACTGGTCATCATTGCCCCT140.11313131313131312No Hit
CGTGACGGGACCAGATACTGGAAGAAACTTCTGTGATCAGAAACCAATACAA140.11313131313131312No Hit
CTATTAGGGATCAGATTCCGATTTGACGGGCAACCAATCAATGAAACAGACA140.11313131313131312No Hit
CGCTTTGGGGAGGTGACATTACATTGTGTTTTTATCTCTTCTTAATTCATGA140.11313131313131312No Hit
ACTTCTGGGATGTCCAGGGTGACAGACTTCACCGGAGATCAGAGATCAGTTA140.11313131313131312No Hit
ATGGTCGGGAGTGTTTGGCCATGTGTATATTTATATCATGTTTACCAAATCT140.11313131313131312No Hit
AATGCGGGGGTCAGATTATTTCTTGACAAGTTACCAGGAAGAACCTTTAATG140.11313131313131312No Hit
GACTTGGGGACAGTTTCTAAAGGCTTGTGAACGTTTTATATTGATAGAAAAT140.11313131313131312No Hit
GAGCACGGGGACAAATTCTCAAATTTTGTTTGTGTTAAAATGTACTTACCTG140.11313131313131312No Hit
TGATCCGGGCCTGGAGTAGAAGTCCATCCTCCCCCCAACCTCCTGACCCATT140.11313131313131312No Hit
GCTCATGGGGCAGCATTTCAAAAAATTGTTCTACATGAACTTGAAAACAATG140.11313131313131312No Hit
CGCTGAGGGACAGCTGCTTCTTTTACATGTGGAAGAACAATGATACTATTTA140.11313131313131312No Hit
TGAGCTAGGGCAGAGAAGGAGGGACTTTTCAGAAATTAGAAAATTGTTGTGA140.11313131313131312No Hit
GCTAGGGGTAATTACAAACTTACTATCCGCCATCCCATACATTGGGACAGAC140.11313131313131312No Hit
TCTCCAGGGAGTGAAACTAGAAGAAGAGCTGTCAGTGGAAGGAAACACAGAC140.11313131313131312No Hit
AATTGAGGGAAGCTACCAAGATCCTGAATGTCAGAGATGTACTCAGAGGGTT130.10505050505050505No Hit
ATACTTGGGATTCAGAAGGAGTGGAAATCAGGGAATAAAGGCATTAAATTAT130.10505050505050505No Hit
ACACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC130.10505050505050505No Hit
TCCAAAGGGGAAATAAGCAATTTTCAGAAAAACTTTGTTTCTTATTAGTATA130.10505050505050505No Hit
TGGTTTGGGGACCTTCTGGGAGATTCTGACAGATTAAGGTTTCACAACAAAC130.10505050505050505No Hit
ATTATCGGGAGATTTTTCAATGGATAATTTCCTAAAATAAAAGTTAGATAAT130.10505050505050505No Hit
TCAAATGGGTGGTCTGGAAGACCGATGTCCACATGCTAAGAGAAGCAGTCAC130.10505050505050505No Hit
TTGATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC130.10505050505050505No Hit
ACAAGCGGGAACCAAGGCACACTCCCCATAAGAGTTTACTGCAGAGAAAGAA130.10505050505050505No Hit
GGAAATGGGTGGAGAAACAGGTTGAAAATATTGTCTTAGATGCAATGTCTCC130.10505050505050505No Hit
ACACACGGGAGTGAAGTGTAATGAAAAACATCTCTAGGAATGTGCTTTAACC130.10505050505050505No Hit
GTCACAGGGAAGGTGGCCGTCAGACCCAAGAGCAACGAACTCAGAGAAATGT130.10505050505050505No Hit
GCTGCGGGTGCATAAGCCTCTTTATAACATGTATCTTTAAAACAATTAAGTC130.10505050505050505No Hit
TACGGGGAATCTGAGAAATGACAAAGGGGAAATAGCCATTAAGATACAGGAA130.10505050505050505No Hit
TATCGCCGGGATGATCATTTAGAGGTTTATTTTGAACAACTGGCAATTCCAC130.10505050505050505No Hit
ATATTTGGGCTCTAGGTTTCAATTCCGTTTGACAGCATTTTGCTCGGAGTAG130.10505050505050505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)