FastQCFastQC Report
Sat 31 Dec 2022
SRR5844469.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844469.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65790
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGCCGTCT6320.9606323149414805No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGC3810.5791153670770633No Hit
TTTGCTGGGACTGACCGAAAAACCAATATGTGTCACCTGTTTATCCATACCC920.13983888128894967No Hit
CAGCGATGGGAACTACAGTTGTTTCTGAGTAGTGGGCCCTTTAGGTGATTTT900.13679890560875513No Hit
TGCATAGGGCACAGACGGGCATGGATCCAATAGTCAGTTTCACGAACTTCCC890.13527891776865783No Hit
GACTCGGGGAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCC850.12919896640826875No Hit
CCAGATGGGGCCTCGCCCCCACTTTTCTAGTTAACTTTTTCCATATCCCTCT850.12919896640826875No Hit
GACAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAG830.12615899072807416No Hit
GGCGTTGGGATTCTCTATATGAGAGCTTAAGCACAGAGGCTGCTCTGCCAGT830.12615899072807416No Hit
AGATGAGGGGTACAAGTCAATTCTATTTAAAGAAGACAAGCTGAAAATAAAA820.1246390028879769No Hit
TCCGCAGGGGTGTGGAAAACTGTTTATTAGTTTCCATCAGGATTTTCTGTTT810.12311901504787962No Hit
TGGTGTGGGGTTCTTCGCCCTGCCATGTTAGCTAAGTTTCGAGTTGCCCGTC810.12311901504787962No Hit
CGTTGAGGGGGTCTCTCAGGTGACTCTATCTTCTGGTTTGCCGGCCATCCCA810.12311901504787962No Hit
GGGCATGGGGTGGTTACAGAAAGGCAACATAGACAGATCAGAAATAGTCAGG800.12159902720778233No Hit
AGTTGCGGGGCCCCTCCCCGCGGTACCTTGCACTTTTCTCCCTCCCTGCCCC800.12159902720778233No Hit
TGTGCTGGGACAGACCAACGTTTCTTATTAATATAGATGCATAAATAACAAA790.12007903936768506No Hit
ATATCGGGGATTTGTTAAGAATTGACTTGTGCAGATTTCAACATGATGAAAT780.11855905152758778No Hit
AACTGAGGGGATCTGGCTTTGGGGATAAAAATAATTGCTTTCTGATGTGTTT780.11855905152758778No Hit
TTAGTGGGGGATGTATATTGGTTTTAAGATAGCTTACAATTTCAGAAACATT780.11855905152758778No Hit
CGTGGAGGGGAGTACCCCCATGTTCCACACGGATCAGGATTGTCTTGAGACT770.1170390636874905No Hit
ACCTCGGGGAACTCACTGTAGCTCACTAAATTAACAGATTAAAGAAGTATAG760.11551907584739322No Hit
TTGGTGGGGCGATCTGAGTTAGGGTATAAAACAAATGTTAACAGAATGGAAT760.11551907584739322No Hit
GGCAGTGGGATAATTTTTTGTTTTCGTTTTTGTTTGGCAAGAACACGAAAGT750.11399908800729594No Hit
CGACGGGGAGACATCAACCTGATCCTTCATTTTATATAAGATACATTTGTTA750.11399908800729594No Hit
GTATACGGGACAGCCACATATTTTATTAGATGGTGCACAATTGGTGTGTAGT750.11399908800729594No Hit
CACAGCGGGACCTAAGAAAAGAAAAGGAACAGAGGAGTGAAGAAGAGAGGAG750.11399908800729594No Hit
GCCTTGGGGAGGAGACTCAAACTAAATAATTTTCTATAGTCCTAGAACTTCT740.11247910016719866No Hit
TGTTTTGGGGAATTCAGAAGGAGCTAGCTGAAATAACCCTTGATCCTCCTCC740.11247910016719866No Hit
AGTTTAGGGATTTTTCCTTCAGTTGGTAACCTTATTGAGAGCAGAGTTAAAA740.11247910016719866No Hit
TTCTGCGGGAACGTACTTGAAATTTAACTATTAAAAAAGTTGGAGGAACTCA740.11247910016719866No Hit
TCGTCGCGGGAAATTTTAATCAAAATGAAGGCTTAGTTCAAACATAAGGAAA740.11247910016719866No Hit
GACATTGGGGTTGCCGTTATTAATATTTTAAAAATATTCAAAGCAATAAATA740.11247910016719866No Hit
TCAGGTGGGATATGAAACAATTCATCTTGATGCAAAATTTTCCTACATAAAC740.11247910016719866No Hit
AACCTGGGGAAGCAATGCAAATGTCTAATTGTCCCCCAGTGGGCATAGTCAG730.11095911232710139No Hit
CGTTTTGGGATAATCAAGGATCCTAGGTGATTGTCAACATTGTGAGTTTTTT720.1094391244870041No Hit
GACATCGGGATCACAATCGGCTTGATTCAGAACCATTTTGGCTATTTCATCA720.1094391244870041No Hit
GAAACAGGGGTATCAGTTCTACCAGGTTTTAGTTTTGTTCTGGTGGAGACAT720.1094391244870041No Hit
GGTATTGGGAGGCTCCTGCCACCATGCCCAGCTAATTTTTTTTTTTTTTTTT720.1094391244870041No Hit
AAATTAGGGAACCAAAACCTGGTTCCCAATCAGAAAACAGGCAAAGAATATT700.10639914880680955No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCCTGTCTCTTATAC690.10487916096671226No Hit
TTAGAGGGCCACCCGAGAGCTAGAGTACAGGCAGCTGCACACGTTACAGAAG690.10487916096671226No Hit
AATTACGGGACCAAAATAGGTTCATGCTTTCAGCGATTGGCAGCAACCTAGA690.10487916096671226No Hit
AATTGAGGGACCATTAATTTAAAAAGGCATCTATTTCTTTATAGAAAGAAAC690.10487916096671226No Hit
AACCGAGGGATTTCAAAAAGACCTTTGTTCAAATTCCAGCCTTGCTGCTTGG680.10335917312661498No Hit
AGCTGCGGGGCATCTGAAAAAAAATCTATAGACTCCAGCTGCTACATTAGAG670.10183918528651771No Hit
GAAGCAGGGATGTGACAGCAAGATAGTTCTTAGTTTCAAATCCAGCAAGCAA670.10183918528651771No Hit
AGTAGCGGGGAAGTTTTAGTTCCTGAAATGTTTAGAATATGAATTTAGAAAA670.10183918528651771No Hit
TTACAGGGGGACTGACGGCTGAGGCCTGCCTCTCCTTCCCAAGCCCCCTTCC670.10183918528651771No Hit
GTATTTGGGAGAACTGTGAAAAATAAATTTCTAAAGTTTATAAGCCACCCTG660.10031919744642043No Hit
TCGCTTGGGAACTGAAAAAGAAATTGCCCTCGAATTCCCGGCCTGCACACAA660.10031919744642043No Hit
TCGCCAGGGATTTACCCTTGTAATTGTGCATAGGGAAGCACTCACCCAATGA660.10031919744642043No Hit
AAAGCAGGGAATCATTAAATTAGCTATTTTCAGCTAACACATTTGTTGTTGC660.10031919744642043No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)