FastQCFastQC Report
Sat 31 Dec 2022
SRR5844468.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844468.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65573
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGCCGTCT6380.9729614322968295No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGC3660.5581565583395605No Hit
GGTATTGGGAGGCTCCTGCCACCATGCCCAGCTAATTTTTTTTTTTTTTTTT980.14945175605813368No Hit
AGTTGCGGGGCCCCTCCCCGCGGTACCTTGCACTTTTCTCCCTCCCTGCCCC890.13572659478748875No Hit
CCAGATGGGGCCTCGCCCCCACTTTTCTAGTTAACTTTTTCCATATCCCTCT880.1342015768685282No Hit
AAAGCAGGGAATCATTAAATTAGCTATTTTCAGCTAACACATTTGTTGTTGC880.1342015768685282No Hit
TCGTCGCGGGAAATTTTAATCAAAATGAAGGCTTAGTTCAAACATAAGGAAA870.13267655894956765No Hit
GAAACAGGGGTATCAGTTCTACCAGGTTTTAGTTTTGTTCTGGTGGAGACAT860.1311515410306071No Hit
TCGCTTGGGAACTGAAAAAGAAATTGCCCTCGAATTCCCGGCCTGCACACAA840.128101505192686No Hit
GGGCATGGGGTGGTTACAGAAAGGCAACATAGACAGATCAGAAATAGTCAGG810.12352645143580437No Hit
AATTACGGGACCAAAATAGGTTCATGCTTTCAGCGATTGGCAGCAACCTAGA810.12352645143580437No Hit
AGATGAGGGGTACAAGTCAATTCTATTTAAAGAAGACAAGCTGAAAATAAAA810.12352645143580437No Hit
ACAGCCGGGAAAGGATAAAAGGAAAATAACCGTCTGCCGATGGTCCGTACTT810.12352645143580437No Hit
CGACGGGGAGACATCAACCTGATCCTTCATTTTATATAAGATACATTTGTTA800.12200143351684384No Hit
GCGAGAGGGACATCCCTTTATTTTGAGTTTTAAGTTGTTATTTTGCTATACA800.12200143351684384No Hit
GACTCGGGGAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCC780.11895139767892274No Hit
CGTTGAGGGGGTCTCTCAGGTGACTCTATCTTCTGGTTTGCCGGCCATCCCA780.11895139767892274No Hit
AAAGCGGGATCTCTGCCCCCTCTGCTGATGCCCCCATGTTCGTCATGGGTGT770.11742637975996217No Hit
TCCGCAGGGGTGTGGAAAACTGTTTATTAGTTTCCATCAGGATTTTCTGTTT770.11742637975996217No Hit
GGCGTTGGGATTCTCTATATGAGAGCTTAAGCACAGAGGCTGCTCTGCCAGT770.11742637975996217No Hit
ACAGTCGGGGGAGAAAGATGGCGGCTACCTTACTAGCTGCTCGGGGAGCCGG770.11742637975996217No Hit
TTTGCTGGGACTGACCGAAAAACCAATATGTGTCACCTGTTTATCCATACCC750.11437634392204107No Hit
TCAGGTGGGATATGAAACAATTCATCTTGATGCAAAATTTTCCTACATAAAC750.11437634392204107No Hit
TGCGATGGGGTGTGGGGAAGTCACTGAAAAGTGCTGCCTAAGGAAGTTTGGA750.11437634392204107No Hit
AGTCTAGGGACCTGTGCAGGGCCCTCTGGGGCTCAGCCCTGAGCTGGCCTCA750.11437634392204107No Hit
TCGCCAGGGATTTACCCTTGTAATTGTGCATAGGGAAGCACTCACCCAATGA740.11285132600308054No Hit
AAATTAGGGAACCAAAACCTGGTTCCCAATCAGAAAACAGGCAAAGAATATT740.11285132600308054No Hit
ATATCGGGGATTTGTTAAGAATTGACTTGTGCAGATTTCAACATGATGAAAT730.11132630808411997No Hit
GAAGCAGGGATGTGACAGCAAGATAGTTCTTAGTTTCAAATCCAGCAAGCAA730.11132630808411997No Hit
GCCTTGGGGAGGAGACTCAAACTAAATAATTTTCTATAGTCCTAGAACTTCT730.11132630808411997No Hit
TTTTGGGGGACTGGGCTTGGAGAGCTTCCAGATGGCTGAATGCATGGAGGCT730.11132630808411997No Hit
AGTAGCGGGGAAGTTTTAGTTCCTGAAATGTTTAGAATATGAATTTAGAAAA720.10980129016515944No Hit
TGTATAGGGATTGTCTATAAGCCCTATTACCTACAAAAGATAAATATGACAT720.10980129016515944No Hit
CTCACCGGGACTTTTACCAAAAATTCTGTGAACATGTAATGTAACTGGCTTT720.10980129016515944No Hit
ACATCTGGGACACCCAGCTAATTTTGTATTTTTGGTAGAGACAGGGTATCTC720.10980129016515944No Hit
CAGCGATGGGAACTACAGTTGTTTCTGAGTAGTGGGCCCTTTAGGTGATTTT720.10980129016515944No Hit
TTATTTGGGTTCACCTTTCAGGTGAAAGGAAAAGTTGTGTGTGTCTCACTTT710.1082762722461989No Hit
GCTCCTGGGGTGGGTAAGGAGAGAAGAGATTTATGTAAGTAACAATTAGTGT710.1082762722461989No Hit
GTGTTGGGAACTTTGAAGGATTCAGTATGTCAAGTGAGAAAAAAATAATGTC710.1082762722461989No Hit
CTTCTTGGGATGACGATGGCAGCTGAGATTAAAAAGCAAATGGGTCCCGGAG710.1082762722461989No Hit
TCATATGGGGGAGCAGCAGCTCCTGAAGGTTCCGTGGGGGTGCGGGGGGTTG690.1052262364082778No Hit
TGATATGGGTTCTCCTGCTACTGCTGCTGCAACAACTTTGACCTTATTTAAT690.1052262364082778No Hit
GACATTGGGGTTGCCGTTATTAATATTTTAAAAATATTCAAAGCAATAAATA680.10370121848931725No Hit
CACAGCGGGACCTAAGAAAAGAAAAGGAACAGAGGAGTGAAGAAGAGAGGAG680.10370121848931725No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCCTGTCTCTTATAC670.1021762005703567No Hit
AGCTGCGGGGCATCTGAAAAAAAATCTATAGACTCCAGCTGCTACATTAGAG670.1021762005703567No Hit
GGCAAAGGGAGAAAAGCAAGAGTAAACCAAAACCAAAATCAGTAAATAAAGG670.1021762005703567No Hit
GCAACTGGGATAGCAATGTAGTTTGTAGAAAGGATTCCTGGATGTTTTACTA670.1021762005703567No Hit
TGTGCTGGGGTAGGAAGGCTGAAAAATGACGCCATGACAAATGAAATTGTCA660.10065118265139615No Hit
GGCTAAGGGATGAGGGAGACGACTGAAAACCACCTGGGGGCAGGACCCACAG660.10065118265139615No Hit
TCCGTAGGGAGATAATTCATGTGCTTTGCTAAAATTTAAAAACCACTGCTTA660.10065118265139615No Hit
TGTCTAGGGATCTAACATACTATCCGAGCTTCGGTCATTTGGAAGAACTGCA660.10065118265139615No Hit
CACGTGGGGAATGTTTACATATGGAATAAAAACCCCATTTGTTTAACATCTT660.10065118265139615No Hit
GCAATGGGGATACTTATAACTTGTTTTAATCTGTATTAGCCACATTTCAAGT660.10065118265139615No Hit
CATCACGGGGCCCACATCTGGGAAGTGCCATAGCCTTGAGTGAATTTATTTG660.10065118265139615No Hit
TTTTTGGGCTTTGCTGGCCCCGAGAAAGGTCGATGACACAGCCGCAAATCTA660.10065118265139615No Hit
ATTAGCGGGGAGTTCTGGAGGATTAAACTACCGAGAATCTTGGGGAAGACAG660.10065118265139615No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)