FastQCFastQC Report
Sat 31 Dec 2022
SRR5844467.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844467.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65138
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGCCGTCT6551.005557431913783No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCTCGTATGC3830.5879824372869907No Hit
GCGAGAGGGACATCCCTTTATTTTGAGTTTTAAGTTGTTATTTTGCTATACA1000.1535202186127913No Hit
ATTAGCGGGGAGTTCTGGAGGATTAAACTACCGAGAATCTTGGGGAAGACAG990.15198501642666337No Hit
ACCTCGGGGAACTCACTGTAGCTCACTAAATTAACAGATTAAAGAAGTATAG900.13816819675151218No Hit
GGGCATGGGGTGGTTACAGAAAGGCAACATAGACAGATCAGAAATAGTCAGG890.13663299456538427No Hit
TTTGCTGGGACTGACCGAAAAACCAATATGTGTCACCTGTTTATCCATACCC860.13202738800700053No Hit
CGTTGAGGGGGTCTCTCAGGTGACTCTATCTTCTGGTTTGCCGGCCATCCCA850.1304921858208726No Hit
TCCGCAGGGGTGTGGAAAACTGTTTATTAGTTTCCATCAGGATTTTCTGTTT840.1289569836347447No Hit
CAGCGATGGGAACTACAGTTGTTTCTGAGTAGTGGGCCCTTTAGGTGATTTT840.1289569836347447No Hit
AACTGAGGGGATCTGGCTTTGGGGATAAAAATAATTGCTTTCTGATGTGTTT830.12742178144861677No Hit
AGTAGCGGGGAAGTTTTAGTTCCTGAAATGTTTAGAATATGAATTTAGAAAA820.12588657926248886No Hit
CTTCTTGGGATGACGATGGCAGCTGAGATTAAAAAGCAAATGGGTCCCGGAG820.12588657926248886No Hit
GTGTTGGGAACTTTGAAGGATTCAGTATGTCAAGTGAGAAAAAAATAATGTC810.12435137707636096No Hit
TTCTGCGGGAACGTACTTGAAATTTAACTATTAAAAAAGTTGGAGGAACTCA800.12281617489023305No Hit
GGTATTGGGAGGCTCCTGCCACCATGCCCAGCTAATTTTTTTTTTTTTTTTT790.12128097270410514No Hit
CACGAAGGGGCATAGTTAATGGTCATAGACTTTATTCCAAAACATAATTGGA780.1197457705179772No Hit
CTATGAGCGGGGTAGAACAAGCAAACAAAAATCATTGAGAACATAGAAAATG780.1197457705179772No Hit
AAATTAGGGAACCAAAACCTGGTTCCCAATCAGAAAACAGGCAAAGAATATT780.1197457705179772No Hit
TCAGGTGGGATATGAAACAATTCATCTTGATGCAAAATTTTCCTACATAAAC750.11514016395959348No Hit
AATTACGGGACCAAAATAGGTTCATGCTTTCAGCGATTGGCAGCAACCTAGA740.11360496177346556No Hit
TTGCTTTGGGGATATTCATATCCTGGAAGTATACATATTTGTTTTTCCAAAG730.11206975958733766No Hit
TTAGAGGGCCACCCGAGAGCTAGAGTACAGGCAGCTGCACACGTTACAGAAG730.11206975958733766No Hit
CGTTTTGGGATAATCAAGGATCCTAGGTGATTGTCAACATTGTGAGTTTTTT710.10899935521508182No Hit
GACATCGGGATCACAATCGGCTTGATTCAGAACCATTTTGGCTATTTCATCA710.10899935521508182No Hit
TGCGTGGGGGAGTTCTCTGCTCTTGGAATTAAAATTGCGTGCCCCCTGTATA700.10746415302895392No Hit
GAAACAGGGGTATCAGTTCTACCAGGTTTTAGTTTTGTTCTGGTGGAGACAT700.10746415302895392No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTCTAAATCCTGTCTCTTATAC690.105928950842826No Hit
CTAATAAGGGAGCTGGAGATAGGAATCAAAGCAAGAAAACTGCATCTTACTT690.105928950842826No Hit
TGCACTGGGGTTTGGGAAAAAGACTGCCTTTATGGAAGGAGAAATGTGGAAT690.105928950842826No Hit
TGCGATGGGGTGTGGGGAAGTCACTGAAAAGTGCTGCCTAAGGAAGTTTGGA690.105928950842826No Hit
AAGCAGGGGGTGGGATATAAATTAGTTTTTATTATTCTCTTAAAAATCAAAG680.1043937486566981No Hit
CGTTTTGGGCATCAAATGAAGTTAGAGAAGTATACTAATCCCAGCAACTATG680.1043937486566981No Hit
AAAGCGGGATCTCTGCCCCCTCTGCTGATGCCCCCATGTTCGTCATGGGTGT670.10285854647057018No Hit
TGTTTTGGGGAATTCAGAAGGAGCTAGCTGAAATAACCCTTGATCCTCCTCC670.10285854647057018No Hit
TGTGCTGGGACAGACCAACGTTTCTTATTAATATAGATGCATAAATAACAAA670.10285854647057018No Hit
TCTAGGGGCGATCTTACGACGGGGTTTGTAAGAAGTATCTTATGTCAAAGTC660.10132334428444227No Hit
GACTCGGGGAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCC660.10132334428444227No Hit
GTTCCGGGGATATTCTAAAAAGTCAAAACGTTAACAATAGTTTTTATCTAAT660.10132334428444227No Hit
TCGTCGCGGGAAATTTTAATCAAAATGAAGGCTTAGTTCAAACATAAGGAAA660.10132334428444227No Hit
ACAGCCGGGAAAGGATAAAAGGAAAATAACCGTCTGCCGATGGTCCGTACTT660.10132334428444227No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)