FastQCFastQC Report
Sat 31 Dec 2022
SRR5844460.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844460.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146502
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGCCGTCT8550.5836097800712617RNA PCR Primer, Index 37 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGC6540.44641028791415815No Hit
GCAGTGGGGGGACAACCCCATGAAAAAGTGGGCAAAGGATATGAACATACAC2460.1679157963713806No Hit
TCAAAGGGGGTACCTGACTGTCGAAAGAAAAACAGAAAGCAACAACAACATC2060.14061241484757886No Hit
AGCAAGGGGGAAACTACCCCTGAAGAAGCACAGACTTCGGATGTACAAAACA1850.12627813954758296No Hit
CCGTTTGGGAGATGAGAGCCACTCGGGAAAAGAGCCATAGTCAACTGGGAGG1820.12423038593329784No Hit
GCATGAGGGAATGCATATTAGTAAAGAATCTGAGATGTCTTAAAGGAACCCG1710.11672195601425236No Hit
AGGACCGGGCTGTGCGCCGCTGCCGCCGCAGCCACCCTGCAGCCTGCCTGGC1530.10443543432854159No Hit
GTCGGCGGGCCTCTGAAAGATTTGTAGAAGGTTAAAGTGGAAAGGGACTTGG1520.10375284979044655No Hit
CTACGAGGGGGGAAAAAATGAAAATAGGAAAAAAGGAAGTGAAAGAGATAAT1510.10307026525235151No Hit
TCTCATGGGGGAGAGAGCGAGTGAGCAAGCGAGCAGAAAAGAGGTGGAGAGG1500.10238768071425645No Hit
TTTAGCGGGCAACACAACCACCCACAGCCTAATTATTAGCATCATCCCTCTA1470.10033992709997132No Hit
TGATCCGGGCGCCTCCCACCTGCCTGAAAGGCTGGCACCAGGACGCTTTGAT1470.10033992709997132No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)