FastQCFastQC Report
Sat 31 Dec 2022
SRR5844459.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844459.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146298
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGCCGTCT7820.5345254207166195RNA PCR Primer, Index 37 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGC6330.4326785055161383No Hit
GCAGTGGGGGGACAACCCCATGAAAAAGTGGGCAAAGGATATGAACATACAC2550.17430176762498462No Hit
TCAAAGGGGGTACCTGACTGTCGAAAGAAAAACAGAAAGCAACAACAACATC1750.11961886013479336No Hit
CCGTTTGGGAGATGAGAGCCACTCGGGAAAAGAGCCATAGTCAACTGGGAGG1740.11893532379116598No Hit
AGCAAGGGGGAAACTACCCCTGAAGAAGCACAGACTTCGGATGTACAAAACA1680.11483410572940163No Hit
GCTGGGAGTGCTTTGATCTGAGTTATCAATAACAGGTAAATGCCATTATGGA1670.11415056938577424No Hit
GCATGAGGGAATGCATATTAGTAAAGAATCTGAGATGTCTTAAAGGAACCCG1650.11278349669851948No Hit
GTAGAGGGGATCCTGATGACAGTGCCTGGTAGTCAATTAAACTATAACTTTC1630.11141642401126467No Hit
TGCATGCGGGAAATCACAGAATACAGCTTTCAAGAAGCATGCACTAGACTGC1620.11073288766763728No Hit
TACGCCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1610.11004935132400989No Hit
TCTCATGGGGGAGAGAGCGAGTGAGCAAGCGAGCAGAAAAGAGGTGGAGAGG1530.10458106057499078No Hit
TCGAGATCGTGCCACTGCTCTCCAGTCTGGGAAACAGAGCAAGACTCCGTCA1530.10458106057499078No Hit
TAGAGAGGGAAAGCATACACTTCAGCCTAAAATGCAGACTTCCAAGTCCATT1500.1025304515441086No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)