FastQCFastQC Report
Sat 31 Dec 2022
SRR5844458.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844458.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145160
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGCCGTCT7650.5270046844860843RNA PCR Primer, Index 37 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGC5790.3988702121796638No Hit
GCAGTGGGGGGACAACCCCATGAAAAAGTGGGCAAAGGATATGAACATACAC2530.1742904381372279No Hit
CCGTTTGGGAGATGAGAGCCACTCGGGAAAAGAGCCATAGTCAACTGGGAGG1900.13089005235602094No Hit
TCAAAGGGGGTACCTGACTGTCGAAAGAAAAACAGAAAGCAACAACAACATC1890.13020115734362084No Hit
AGCAAGGGGGAAACTACCCCTGAAGAAGCACAGACTTCGGATGTACAAAACA1800.12400110223201985No Hit
GCATGAGGGAATGCATATTAGTAAAGAATCTGAGATGTCTTAAAGGAACCCG1590.10953430697161752No Hit
TCTCATGGGGGAGAGAGCGAGTGAGCAAGCGAGCAGAAAAGAGGTGGAGAGG1520.10471204188481677No Hit
CTACGAGGGGGGAAAAAATGAAAATAGGAAAAAAGGAAGTGAAAGAGATAAT1460.10057867181041608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)