FastQCFastQC Report
Sat 31 Dec 2022
SRR5844457.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844457.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114404
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGCCGTCT7500.6555714835145624RNA PCR Primer, Index 37 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGC6960.6083703367015139No Hit
AACGTTGGGACCACCCACTGACATCTTTTTCCAAAATACATTTTAACTACCC1950.17044858571378624No Hit
TTGTTGGGGGGATACCAGAGAGGCATTGGTCAAAAAATTTGGTGCTCAGAAT1790.15646306073214222No Hit
TGCAGAGGGAGCTTTTTGGGGAACTCTTTAAAATTGGCGCCAAGATCCGGTG1590.13898115450508725No Hit
TGAACGCGGGATGACAACTTTGGTATCGTGGAAGGACTCATGACCACAGTCC1580.1381070591937345No Hit
TAAGAAGGGGTGTACCAGTGGACTTTGAAGAGGTGCACGTGAGTTCCAATGC1580.1381070591937345No Hit
CATTGTGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1580.1381070591937345No Hit
TCCTGCGGGAGAGCTGTGCAGCTGAGGCGCCGCCGTGGAGCCGCCTTGGAGC1570.13723296388238174No Hit
AAGTTGGGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAA1560.13635886857102897No Hit
GTAGGGGGGAAGCTCAGAGGCACCTTCTCCTGTCAACGCCATGAATTCCTTT1550.13548477325967623No Hit
AAGCGTGGGCAGTGTTAATTGTGCCTCATTATTCTCTTAGGAATTTGCTGAT1530.13373658263697075No Hit
CTGGCTGGGGGGGTCGACTGACGGTAACGGGGCAGAGAGGCTGTTCGCAGAG1510.13198839201426524No Hit
CAGCAGCAGCAGGAAGGATCACTCGGTGAACAAGATGTGTGCCTCCTCCTGC1510.13198839201426524No Hit
GTTGGCGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA1500.1311142967029125No Hit
GTCCAAGGGCGATCTATTGTGGGGGATTTCTCTGTATTAAGTGATTTATAGC1500.1311142967029125No Hit
CGACGCGGGAAGAACATGATGGCCGCCTGCGACCCGCGCCACGGCCGCTACC1470.12849201076885422No Hit
ATCGAGGGGACAGCCCAAGAGGACTGAGACTGGCACTTAGCCCAAGAGGTCT1440.12586972483479597No Hit
CGGGATGGGACCTCTAAATTTCAAAATTATGAAGAATTACAGAATTACTCAT1440.12586972483479597No Hit
CTGCTGGGGATCTTAGCTCGGGGAAGAAAAGAATCACAGTACTTTCCAGCAG1440.12586972483479597No Hit
TAATGAGGGATGAAAGTAATCAATAGCTATGGCAATACAAGAATTGTACTCC1430.12499562952344323No Hit
GCGTCGGGGATGGGCTGATAACCAAATTTTAACACATCAGCATTCACCACCA1420.12412153421209049No Hit
CTCAGGGAGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCA1410.12324743890073775No Hit
TTTTATGGGTCAGCACCATGGAAGAACTTGTAGAACATTACAAAAAGGCACC1410.12324743890073775No Hit
TTAGAAGGGGAGTGCGGACATTGTCAGCTGCGTTTCCGCGGTCGCGGGTGAG1390.12149924827803224No Hit
CTAATAGGGTCCAGGAATGAATGAATAAATCAGATTTTCCTGGTACCCCACA1390.12149924827803224No Hit
AACAACGGGTAGTCGGCAGGACTCATTTTTTCATCATGGCTGGCTGATTTCT1390.12149924827803224No Hit
TAGTGTGGGGGGTTTACATGTGGAAGACTTTAATACAGAAGTTTTCCATGTT1380.12062515296667947No Hit
TCCACTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1380.12062515296667947No Hit
GCTCAAGGGAACACCTTCCTGGAGCGCCTGTTCACAACCAGGAGTTTCGACA1380.12062515296667947No Hit
TGTCGGGGGGCCTTGCAGGACCGCTGGGGAGGATGTTGTTAACGTAAAGAGC1360.11887696234397398No Hit
TGGTCGGGGGAAAAATTGATTAAATAAAAATTGATATGTATCCAGAGTCATC1360.11887696234397398No Hit
TTAGCAGGGGATAATGAAAGAGTGAAGAACCTTTCAGATAAAATGTAAGCTG1360.11887696234397398No Hit
TTAAGTGGGCACGACGCTCTTCCGTTCTACCGTTGGGATAGGTTTGGTCCTA1350.11800286703262124No Hit
GGTAGCGGGGGGCCTATCCTAGTGACCTCACTTTGACTTGATTGCCTTTGTA1330.11625467640991574No Hit
GGTGTAGGGCCTTTGTAGTGTTGTAAATACTTTTGCTTTGAAAACTTTTTCA1320.11538058109856299No Hit
GTTAATGGGGCCTCTCATAAATATTCATAAGATTATCCTATAACCTACTAAA1300.11363239047585749No Hit
AAAATTGGACTAAAAATGGACGCCTGGCAACCAAGTAAGTTCTTCCTTTTCC1290.11275829516450472No Hit
TTGCTAGGGAGGAAGTAGATATGAAAAATATCAACATGGATAATGATCTGGA1290.11275829516450472No Hit
CAGGAAGGCTGATGATGACCTATGGGGCAAACCAAAAAGTCATGGTCTATAT1290.11275829516450472No Hit
TCAATAGGGCACGACGCTCTTCCGTGGTCATGGGGAGGATAACCACTATTTT1280.11188419985315198No Hit
GGCAATGGGATTCTTGGTCATCTCGACTAATTCTGAGGCAATGATGGACAGA1280.11188419985315198No Hit
TTCCAGGGAGAGCTAACTGATAAGAGTGGAGGAGGAATGCCTTCTCCTAAGA1280.11188419985315198No Hit
GGCTTTGGGGGAGTGGGCCGGAGATGGCGGCGGCCGACGGGGCTTTGCCGGA1270.11101010454179923No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGGAAT1260.11013600923044649No Hit
AGGCAGGGATAACCAAGTACAAACATTGATGTATAATGACAGTATAAAATGC1260.11013600923044649No Hit
GGTTCTGGGTTCTTTGAAAATCCAAGACAGTCAGAAATATTTGAAAAAGCAG1250.10926191391909373No Hit
GCTGACGGGATACATGTGCAAGTGTGCCGAGGGCTATGGAGGGGACTTGTGT1250.10926191391909373No Hit
GACTTTGGGTCTGCCACAGCAAGGCTGTCGGGGTCAAGCTGGAAAGGCCAGC1250.10926191391909373No Hit
AGATTCGGGTTACAACCTGAAAATGCAAGGGAGTTAACAAGCCAAAGACAAA1240.108387818607741No Hit
AATATAGGGAGGCTAATTGCCAGGTATGCGGCAAGGCTGGCAGCAGAGTCCT1240.108387818607741No Hit
GGCTAGGGGGCCAACCAACGTGCAACAAAGATGCTGGGAAGTGGTTAAGTGT1240.108387818607741No Hit
ATGCCAGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1230.10751372329638824No Hit
CAAGTGGGGAACGCCCAAAATAATTAGTAGGAGTTCATCTTTAAAGGGGATA1220.1066396279850355No Hit
GGTAATGGGGACGAGGATGATGATTCAGCAGATATGGATGAATCAGATGAAG1220.1066396279850355No Hit
CTCATTGGGGGACTCATTCCCGGGCACACCTATGGCTCCTTCGGTATCACCT1220.1066396279850355No Hit
GTACAGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1220.1066396279850355No Hit
AGCCAAGGGACCTGTCCCAGCCACAGGCTTCTTGTCCACCTCTTCTCCCCTG1200.10489143736232998No Hit
GTTAATGGGGGACACTCCTGGGAAAGCCTGACTCAATTATGAATGCTTCATG1190.10401734205097724No Hit
CCAAGAGGGGATATGACACTTGGTTATAACTCTTTAGTTGGATATAACATTA1190.10401734205097724No Hit
TCTTTAGGGCGCCGCCGTGGTAGCAGCCTCAGCCGTTTCTGGAGTCTCGGGC1190.10401734205097724No Hit
TGCCAGGGGGAAGCAGTGAAAAATATCTGATGTGAAGACATGGGAAGGGCAT1190.10401734205097724No Hit
GCTAGCGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAG1190.10401734205097724No Hit
TACTTCGGGAAGATGTATGATGTTGGGTTTAACATCATATGTTCACAGTGTT1170.10226915142827174No Hit
TTACGTGGGATATTTATGTACTCTCTAAGAAGACAAATGAGGTCATAAACAC1170.10226915142827174No Hit
GCTGTCGGGCCACTGCATTCTTTGGCTAAGGCCTGAATGCTTGCTCATCTGT1170.10226915142827174No Hit
TTAATCGGGATATGAAAAGCATACAGTTTGGTTGTCTAAATCTGTAATTGTG1160.10139505611691899No Hit
ATTGCAGGGATTTTTTATAGAGACTAGGTTTCGCCTTGTTGCCCAGGCTGGA1160.10139505611691899No Hit
ATCTTTGGGTGGCGTAGGGAACCCTAAGTGGTTTTATCATTTTATATGTTAA1150.10052096080556625No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)