FastQCFastQC Report
Sat 31 Dec 2022
SRR5844456.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844456.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113498
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGCCGTCT7040.6202752471409188RNA PCR Primer, Index 37 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGC6850.6035348640504679No Hit
TAAGAAGGGGTGTACCAGTGGACTTTGAAGAGGTGCACGTGAGTTCCAATGC1980.1744524132583834No Hit
ATCGAGGGGACAGCCCAAGAGGACTGAGACTGGCACTTAGCCCAAGAGGTCT1890.1665227581102751No Hit
AAGTTGGGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAA1830.1612363213448695No Hit
AACGTTGGGACCACCCACTGACATCTTTTTCCAAAATACATTTTAACTACCC1760.1550688117852297No Hit
TGCAGAGGGAGCTTTTTGGGGAACTCTTTAAAATTGGCGCCAAGATCCGGTG1700.14978237501982414No Hit
AACAACGGGTAGTCGGCAGGACTCATTTTTTCATCATGGCTGGCTGATTTCT1700.14978237501982414No Hit
TGAACGCGGGATGACAACTTTGGTATCGTGGAAGGACTCATGACCACAGTCC1640.1444959382544186No Hit
CAGCAGCAGCAGGAAGGATCACTCGGTGAACAAGATGTGTGCCTCCTCCTGC1630.14361486546018432No Hit
TTAGCAGGGGATAATGAAAGAGTGAAGAACCTTTCAGATAAAATGTAAGCTG1630.14361486546018432No Hit
TCCTGCGGGAGAGCTGTGCAGCTGAGGCGCCGCCGTGGAGCCGCCTTGGAGC1610.1418527198717158No Hit
CATTGTGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1580.139209501489013No Hit
AGCCAAGGGACCTGTCCCAGCCACAGGCTTCTTGTCCACCTCTTCTCCCCTG1560.1374473559005445No Hit
CTCAGGGAGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCA1530.13480413751784173No Hit
ATGCCAGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1520.13392306472360746No Hit
TTGTTGGGGGGATACCAGAGAGGCATTGGTCAAAAAATTTGGTGCTCAGAAT1500.13216091913513894No Hit
TGGTCGGGGGAAAAATTGATTAAATAAAAATTGATATGTATCCAGAGTCATC1470.12951770075243615No Hit
TTGCTAGGGAGGAAGTAGATATGAAAAATATCAACATGGATAATGATCTGGA1440.1268744823697334No Hit
AAAATTGGACTAAAAATGGACGCCTGGCAACCAAGTAAGTTCTTCCTTTTCC1430.12599340957549912No Hit
GTGCAGGGTGGCATATTTCATCACAAGTTGCTGAACTTAAGAAACTTGTGGT1430.12599340957549912No Hit
TGCCAGGGGGAAGCAGTGAAAAATATCTGATGTGAAGACATGGGAAGGGCAT1430.12599340957549912No Hit
AAGCGTGGGCAGTGTTAATTGTGCCTCATTATTCTCTTAGGAATTTGCTGAT1430.12599340957549912No Hit
GTAGGGGGGAAGCTCAGAGGCACCTTCTCCTGTCAACGCCATGAATTCCTTT1420.12511233678126488No Hit
CGGGATGGGACCTCTAAATTTCAAAATTATGAAGAATTACAGAATTACTCAT1410.1242312639870306No Hit
GCTCAAGGGAACACCTTCCTGGAGCGCCTGTTCACAACCAGGAGTTTCGACA1400.12335019119279633No Hit
TTAATCGGGATATGAAAAGCATACAGTTTGGTTGTCTAAATCTGTAATTGTG1390.1224691183985621No Hit
GTTAATGGGGCCTCTCATAAATATTCATAAGATTATCCTATAACCTACTAAA1380.12158804560432783No Hit
TTTTATGGGTCAGCACCATGGAAGAACTTGTAGAACATTACAAAAAGGCACC1380.12158804560432783No Hit
TCTTTAGGGCGCCGCCGTGGTAGCAGCCTCAGCCGTTTCTGGAGTCTCGGGC1380.12158804560432783No Hit
TACTTGGGTAATAATGGCATTGATAGAAGAAAACAAGAAAATTTCAAGAAGA1370.12070697281009357No Hit
GGTTCTGGGTTCTTTGAAAATCCAAGACAGTCAGAAATATTTGAAAAAGCAG1360.11982590001585931No Hit
TTCCAGGGAGAGCTAACTGATAAGAGTGGAGGAGGAATGCCTTCTCCTAAGA1360.11982590001585931No Hit
CTAATAGGGTCCAGGAATGAATGAATAAATCAGATTTTCCTGGTACCCCACA1350.11894482722162505No Hit
TAATGAGGGATGAAAGTAATCAATAGCTATGGCAATACAAGAATTGTACTCC1340.11806375442739078No Hit
AGATTCGGGTTACAACCTGAAAATGCAAGGGAGTTAACAAGCCAAAGACAAA1330.11718268163315652No Hit
TGTCGGGGGGCCTTGCAGGACCGCTGGGGAGGATGTTGTTAACGTAAAGAGC1330.11718268163315652No Hit
GGCTTTGGGGGAGTGGGCCGGAGATGGCGGCGGCCGACGGGGCTTTGCCGGA1320.11630160883892228No Hit
CTCATTGGGGGACTCATTCCCGGGCACACCTATGGCTCCTTCGGTATCACCT1320.11630160883892228No Hit
TATTTAGGGAGAGCCAGCTGATTTCACAAGTATTTACAAAGTGTTACCAGAG1310.11542053604468802No Hit
CGACGCGGGAAGAACATGATGGCCGCCTGCGACCCGCGCCACGGCCGCTACC1310.11542053604468802No Hit
GCGTCGGGGATGGGCTGATAACCAAATTTTAACACATCAGCATTCACCACCA1300.11453946325045375No Hit
GGTAATGGGGACGAGGATGATGATTCAGCAGATATGGATGAATCAGATGAAG1300.11453946325045375No Hit
GCCAGAAGGGAGCTGCAGAAGGACAAGCAGGTCTACCGGGCCACGCACCGCC1300.11453946325045375No Hit
CAGGAAGGCTGATGATGACCTATGGGGCAAACCAAAAAGTCATGGTCTATAT1290.11365839045621949No Hit
CTGGCTGGGGGGGTCGACTGACGGTAACGGGGCAGAGAGGCTGTTCGCAGAG1290.11365839045621949No Hit
TCCACTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1290.11365839045621949No Hit
TTAGAAGGGGAGTGCGGACATTGTCAGCTGCGTTTCCGCGGTCGCGGGTGAG1280.11277731766198523No Hit
GTTGGCGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA1280.11277731766198523No Hit
GCGCATGGGTCAAAATACTGATTACAGATGTACAATTTAGCTTAATCAGAAA1280.11277731766198523No Hit
GTACAGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1270.11189624486775097No Hit
CACAAAGGGAGACCCACCCACTGCCAAGGCTGCCGTGGAATGGTTTGATGGG1260.1110151720735167No Hit
GCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATA1250.11013409927928247No Hit
CAAGTGGGGAACGCCCAAAATAATTAGTAGGAGTTCATCTTTAAAGGGGATA1230.10837195369081394No Hit
CCAAGAGGGGATATGACACTTGGTTATAACTCTTTAGTTGGATATAACATTA1220.10749088089657968No Hit
TTAAGTGGGCACGACGCTCTTCCGTTCTACCGTTGGGATAGGTTTGGTCCTA1220.10749088089657968No Hit
GTCCAAGGGCGATCTATTGTGGGGGATTTCTCTGTATTAAGTGATTTATAGC1220.10749088089657968No Hit
ATCTTTGGGTGGCGTAGGGAACCCTAAGTGGTTTTATCATTTTATATGTTAA1220.10749088089657968No Hit
GGTAGCGGGGGGCCTATCCTAGTGACCTCACTTTGACTTGATTGCCTTTGTA1220.10749088089657968No Hit
TAGTGTGGGGGGTTTACATGTGGAAGACTTTAATACAGAAGTTTTCCATGTT1200.10572873530811115No Hit
GCCGAGGGTGCAGACTGATGCTCTTCCCTGATGGAGACCCTGAGATCTTCCC1200.10572873530811115No Hit
GCTGACGGGATACATGTGCAAGTGTGCCGAGGGCTATGGAGGGGACTTGTGT1190.1048476625138769No Hit
GGTGTAGGGCCTTTGTAGTGTTGTAAATACTTTTGCTTTGAAAACTTTTTCA1180.10396658971964265No Hit
TACTTCGGGAAGATGTATGATGTTGGGTTTAACATCATATGTTCACAGTGTT1180.10396658971964265No Hit
TCAATAGGGCACGACGCTCTTCCGTGGTCATGGGGAGGATAACCACTATTTT1170.10308551692540839No Hit
CTGCTGGGGATCTTAGCTCGGGGAAGAAAAGAATCACAGTACTTTCCAGCAG1170.10308551692540839No Hit
AATATAGGGAGGCTAATTGCCAGGTATGCGGCAAGGCTGGCAGCAGAGTCCT1170.10308551692540839No Hit
GTTAATGGGGGACACTCCTGGGAAAGCCTGACTCAATTATGAATGCTTCATG1160.10220444413117412No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)