FastQCFastQC Report
Sat 31 Dec 2022
SRR5844455.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844455.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112252
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGCCGTCT6890.6137975269928375RNA PCR Primer, Index 37 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGC6740.6004347361294231No Hit
ATCGAGGGGACAGCCCAAGAGGACTGAGACTGGCACTTAGCCCAAGAGGTCT1870.16658945943056694No Hit
AAGTTGGGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAA1760.156790079464063No Hit
GGTTCTGGGTTCTTTGAAAATCCAAGACAGTCAGAAATATTTGAAAAAGCAG1710.15233581584292485No Hit
TGGTCGGGGGAAAAATTGATTAAATAAAAATTGATATGTATCCAGAGTCATC1710.15233581584292485No Hit
AACGTTGGGACCACCCACTGACATCTTTTTCCAAAATACATTTTAACTACCC1690.1505541103944696No Hit
TTAGCAGGGGATAATGAAAGAGTGAAGAACCTTTCAGATAAAATGTAAGCTG1570.139863877703738No Hit
TTGTTGGGGGGATACCAGAGAGGCATTGGTCAAAAAATTTGGTGCTCAGAAT1560.1389730249795104No Hit
TAAGAAGGGGTGTACCAGTGGACTTTGAAGAGGTGCACGTGAGTTCCAATGC1530.13630046680682747No Hit
TAGTGTGGGGGGTTTACATGTGGAAGACTTTAATACAGAAGTTTTCCATGTT1500.13362790863414462No Hit
CTGGCTGGGGGGGTCGACTGACGGTAACGGGGCAGAGAGGCTGTTCGCAGAG1500.13362790863414462No Hit
AAGCGTGGGCAGTGTTAATTGTGCCTCATTATTCTCTTAGGAATTTGCTGAT1490.13273705590991697No Hit
TGAACGCGGGATGACAACTTTGGTATCGTGGAAGGACTCATGACCACAGTCC1470.13095535046146173No Hit
CTGCTGGGGATCTTAGCTCGGGGAAGAAAAGAATCACAGTACTTTCCAGCAG1470.13095535046146173No Hit
TGCAGAGGGAGCTTTTTGGGGAACTCTTTAAAATTGGCGCCAAGATCCGGTG1450.12917364501300643No Hit
AACAACGGGTAGTCGGCAGGACTCATTTTTTCATCATGGCTGGCTGATTTCT1450.12917364501300643No Hit
GTTGGCGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA1440.1282827922887788No Hit
TCCTGCGGGAGAGCTGTGCAGCTGAGGCGCCGCCGTGGAGCCGCCTTGGAGC1430.1273919395645512No Hit
TTTTATGGGTCAGCACCATGGAAGAACTTGTAGAACATTACAAAAAGGCACC1420.12650108684032357No Hit
CTAATAGGGTCCAGGAATGAATGAATAAATCAGATTTTCCTGGTACCCCACA1420.12650108684032357No Hit
CAGCAGCAGCAGGAAGGATCACTCGGTGAACAAGATGTGTGCCTCCTCCTGC1410.12561023411609593No Hit
CATTGTGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1410.12561023411609593No Hit
GTCCAAGGGCGATCTATTGTGGGGGATTTCTCTGTATTAAGTGATTTATAGC1400.1247193813918683No Hit
TAATGAGGGATGAAAGTAATCAATAGCTATGGCAATACAAGAATTGTACTCC1390.12382852866764066No Hit
GTACAGGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1390.12382852866764066No Hit
AAAATTGGACTAAAAATGGACGCCTGGCAACCAAGTAAGTTCTTCCTTTTCC1380.12293767594341302No Hit
TTCCAGGGAGAGCTAACTGATAAGAGTGGAGGAGGAATGCCTTCTCCTAAGA1380.12293767594341302No Hit
GCCAGAAGGGAGCTGCAGAAGGACAAGCAGGTCTACCGGGCCACGCACCGCC1370.1220468232191854No Hit
TTAATCGGGATATGAAAAGCATACAGTTTGGTTGTCTAAATCTGTAATTGTG1360.12115597049495777No Hit
ATCTTTGGGTGGCGTAGGGAACCCTAAGTGGTTTTATCATTTTATATGTTAA1360.12115597049495777No Hit
GTAGGGGGGAAGCTCAGAGGCACCTTCTCCTGTCAACGCCATGAATTCCTTT1340.11937426504650252No Hit
GGTAGCGGGGGGCCTATCCTAGTGACCTCACTTTGACTTGATTGCCTTTGTA1340.11937426504650252No Hit
GTTAATGGGGCCTCTCATAAATATTCATAAGATTATCCTATAACCTACTAAA1320.11759255959804724No Hit
GGTGTAGGGCCTTTGTAGTGTTGTAAATACTTTTGCTTTGAAAACTTTTTCA1310.11670170687381962No Hit
TACTTGGGTAATAATGGCATTGATAGAAGAAAACAAGAAAATTTCAAGAAGA1310.11670170687381962No Hit
CGGGATGGGACCTCTAAATTTCAAAATTATGAAGAATTACAGAATTACTCAT1290.11492000142536436No Hit
CCAAGAGGGGATATGACACTTGGTTATAACTCTTTAGTTGGATATAACATTA1270.11313829597690911No Hit
AATATAGGGAGGCTAATTGCCAGGTATGCGGCAAGGCTGGCAGCAGAGTCCT1270.11313829597690911No Hit
GGCTTTGGGGGAGTGGGCCGGAGATGGCGGCGGCCGACGGGGCTTTGCCGGA1260.11224744325268148No Hit
GCGCATGGGTCAAAATACTGATTACAGATGTACAATTTAGCTTAATCAGAAA1260.11224744325268148No Hit
TGCCAGGGGGAAGCAGTGAAAAATATCTGATGTGAAGACATGGGAAGGGCAT1260.11224744325268148No Hit
ATGCCAGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1250.11135659052845383No Hit
TTGCTAGGGAGGAAGTAGATATGAAAAATATCAACATGGATAATGATCTGGA1240.1104657378042262No Hit
TACTTCGGGAAGATGTATGATGTTGGGTTTAACATCATATGTTCACAGTGTT1240.1104657378042262No Hit
AGATTCGGGTTACAACCTGAAAATGCAAGGGAGTTAACAAGCCAAAGACAAA1230.10957488507999857No Hit
GCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATA1220.10868403235577094No Hit
GTGCAGGGTGGCATATTTCATCACAAGTTGCTGAACTTAAGAAACTTGTGGT1220.10868403235577094No Hit
GTAACTGGGCTCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAG1220.10868403235577094No Hit
GCTCAAGGGAACACCTTCCTGGAGCGCCTGTTCACAACCAGGAGTTTCGACA1220.10868403235577094No Hit
TTAGAAGGGGAGTGCGGACATTGTCAGCTGCGTTTCCGCGGTCGCGGGTGAG1210.10779317963154332No Hit
TTACGGGGGTGAGCTAAAGATTCATTATTCCTGTTTCAAAATTTATTCCTAT1210.10779317963154332No Hit
TAAACTGGGGGGGGCTGGGAGCCCTGGGCGTGTAGTGTAACTGGAATCTTTT1210.10779317963154332No Hit
CTCATTGGGGGACTCATTCCCGGGCACACCTATGGCTCCTTCGGTATCACCT1210.10779317963154332No Hit
CAAGTGGGGAACGCCCAAAATAATTAGTAGGAGTTCATCTTTAAAGGGGATA1200.10690232690731569No Hit
GGCAATGGGATTCTTGGTCATCTCGACTAATTCTGAGGCAATGATGGACAGA1200.10690232690731569No Hit
CACAAAGGGAGACCCACCCACTGCCAAGGCTGCCGTGGAATGGTTTGATGGG1200.10690232690731569No Hit
TATTTAGGGAGAGCCAGCTGATTTCACAAGTATTTACAAAGTGTTACCAGAG1200.10690232690731569No Hit
CGACGCGGGAAGAACATGATGGCCGCCTGCGACCCGCGCCACGGCCGCTACC1190.10601147418308807No Hit
TCTTTAGGGCGCCGCCGTGGTAGCAGCCTCAGCCGTTTCTGGAGTCTCGGGC1180.1051206214588604No Hit
ATTGCAGGGATTTTTTATAGAGACTAGGTTTCGCCTTGTTGCCCAGGCTGGA1180.1051206214588604No Hit
CAATGTCGGGATGCAAAATGATGGAAGGAAAGAAAAGCCAGTTCACTTTAAA1180.1051206214588604No Hit
TCACCTGGGTACTGTGAAATTTTGTCAGAATAGAATCTGAATATACTGAGTT1170.10422976873463279No Hit
AATTTTGGGATATGGAAATGTTGAAAACTATTGTAAAGTAGTATTATGAAGT1170.10422976873463279No Hit
GCGTAAGGGGTCCCAGCTACTGAGGAGGCTGAGGTGGGAGGATTGCTTGAGC1170.10422976873463279No Hit
ATACTTGGGAACCTCTACAAGAAAGTTGATAAACATTTATGTGAAGAAGAGA1160.10333891601040515No Hit
AAATCTGGGGTGAACACAAAGCATTTTGTGTAAGGAGAACCCCTTTCTTTTA1150.10244806328617753No Hit
AGCCAAGGGACCTGTCCCAGCCACAGGCTTCTTGTCCACCTCTTCTCCCCTG1150.10244806328617753No Hit
GGTAATGGGGACGAGGATGATGATTCAGCAGATATGGATGAATCAGATGAAG1150.10244806328617753No Hit
TTGAAAGGGGTTTATGTCAGGAAATGAAAAAGATACTGTACCAAATCTGGAA1150.10244806328617753No Hit
TTAAGTGGGCACGACGCTCTTCCGTTCTACCGTTGGGATAGGTTTGGTCCTA1140.1015572105619499No Hit
GGTATTGGGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATA1140.1015572105619499No Hit
GCTGACGGGATACATGTGCAAGTGTGCCGAGGGCTATGGAGGGGACTTGTGT1130.10066635783772228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)