FastQCFastQC Report
Sat 31 Dec 2022
SRR5844448.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844448.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110770
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGCCGTCT7200.6499954861424574RNA PCR Primer, Index 37 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGC6660.601245824681773No Hit
ATACTAGGGGAATGGGAATAAAGAAAAGCTGAATGACAGGATAAGAGAGGTT1750.1579850139929584No Hit
ACATTAGGGAGGAGATTGTGATCTAGTAAATGTGTCTGGCTCAGTCATTCAG1650.14895729890764647No Hit
TTGGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1540.13902681231380337No Hit
GGAACTGGGCAGGAACTGAATTTAGGAAAGGATTGTCCTTGGGGCATTTGTC1540.13902681231380337No Hit
TCAATCGGGAGTGTCAGGACTCTGCCAGTTACTTTCAGATGTTTCAGGGGAA1540.13902681231380337No Hit
ACCACCGGGTTTCAGCAAACATTTATGAAGCTCCTGTAATATTCAAACCTCC1530.13812404080527219No Hit
AACTAAGGGTTGTGACTACTTGTTGAAATGTGAGTAAAAGTACTTTGTGGCA1480.13361018326261623No Hit
CTCCTAGGGACAGTGCAGCTCCACATTAACTCTACAGACCAAACCATTTGTA1470.13270741175408504No Hit
ACTGGTGGGCCACTAAATGATACAGCTGAATGAAGAAAGAATGCTGGAGAAA1420.12819355421142908No Hit
AGCCTTGGGAACCAAAGCCATCTTGAGCAAAAAAACAAAGCTGGAGGTGTTC1410.1272907827028979No Hit
AAAATAGGGCAACATCCTGAATGTTAGAAGCCTTCATACACTTGTGAAATTG1400.1263880111943667No Hit
CAAAAGGGCAAATCCAAGAGTTATTGGCCTTAGAGAGGAGGTAAGAAAAGAA1390.1254852396858355No Hit
CGTTGCGGGATCCCACGTGCCTGCCAATCTGGAAATCAACACTGATGCAACA1360.12277692516024193No Hit
TCAGCTGGGAGAAGGTAGACAGCTGTAACCTCTAACATTGATACTTCGATCT1360.12277692516024193No Hit
ACTGATGGGATTGTTTAGACAAAATCATAATCTCAGTGTATTTTTTCTTGGG1350.12187415365171077No Hit
TGTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1350.12187415365171077No Hit
CAATCCGGGTGGCCCAATAGCCTTTGCTTGCAAGCTCATCTGTAAATTTAAA1340.12097138214317957No Hit
TTTGTAGGGCTACTGATGGTGGCCATCATGCTGGGTGTCTGCTCCATCATGG1330.12006861063464838No Hit
CTTAGCGGGTTGTTGGATCTTAGGATAAAGTTGGTTGTGTCCAGAGGTGACT1320.11916583912611718No Hit
TTTAGAGGGTCTCTCAAACTATGGAAGTTGCATTGACATCTTCTCTGAATGC1320.11916583912611718No Hit
GCCAAAGGGATCCAACTGAAGCCCGATGTGGTCATCACTGAAAAGGGCATCT1310.118263067617586No Hit
AATGCCGGGATTAGGTCAGCATGTGAAATGTTGGATTTGACATGTCAGGTAG1300.1173602961090548No Hit
GCAGCCGGGATCAAAGGCAAATATTACTTAATGGCGCACATTGGCAGAGGAA1300.1173602961090548No Hit
AGAAAGGGATAGCCATGGTTTTCAAACTGCCGCTCTAAAGCTAGGGAAAATT1300.1173602961090548No Hit
GATCAAGGGAAATATTGTGGGTGTTGCATAAATTGCAGAGATATTTGTATTA1290.11645752460052361No Hit
ACAAGAGGGACTGAAAATCTGCTGCCAGATATACTGAAGCTCCATTGTATGT1290.11645752460052361No Hit
AGTACAGGGGATAACGGCGGCGAGCGGACGGCTGCATTTACGGGGTCTCCCG1270.11465198158346122No Hit
TTCGAGGGGGAAGAAATGCTGCTGGCTCTATTTTTACATTCCCTTCCACCTC1270.11465198158346122No Hit
CTAGTAGGGAGCATTGAGCAGAAGACATCTGCAGACGGCAATGAGAAGAAGA1260.11374921007493004No Hit
GTACATGGGATGAAGATTTCCTGCTTTAGGCTTGAGTTTGAATTGGAATTTT1250.11284643856639884No Hit
CTTGTTGGGATCCCTGGTGCAAAATTACTAAGAGTGAATAGAAGGCTAGAAA1250.11284643856639884No Hit
TTTATTGGGAGATTCCTTTGTTGGACTAATGGGCAAAAGAGCTTTAAATTCT1240.11194366705786765No Hit
CAACGCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1240.11194366705786765No Hit
TTCAGAGGGAGTGTAATGAATGTGGAAAGACCTTTTTTCGGAAGTCAGCCCT1240.11194366705786765No Hit
CACGGAGGGGAGCCGGGGAGGTGATCTGTATAGACGACTGACTGGATCCCAG1230.11104089554933645No Hit
ATTTAAGGGAACTCCCTCAAGAGGTACGTAGAGGACATTTAAGGGAAGCCTA1230.11104089554933645No Hit
AGTAGCGGGGCGGGCGAGAACGGCGGCTGTGTGCGCGCTCGCAGGCCAGCAT1220.11013812404080527No Hit
GCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATA1210.10923535253227407No Hit
TGACCTGGGGATTTTTCTTTCACATTGCAAGTGGGAGAGCAAAAGTGTTTTA1210.10923535253227407No Hit
GGATAAGGGATATATGTCTTGTGTGCGTGTCCTTAAACTTCCAATCTTACTT1210.10923535253227407No Hit
ATTGAAGGGCAAGACTGTCTTTATTTTACCGGATTGGAGCTTGCCAGGTGTG1210.10923535253227407No Hit
CTCATAGGGAGGTTTTATTCACAGCTTATTCAGCATCAGATAATTCATACTG1200.10833258102374288No Hit
CACAATGGGTCAATTCAACAAAAAAACCTAACTATCTTAAATATATATGCAC1200.10833258102374288No Hit
TTGAACGGGGTAATTACAAACTTACTATCCGCCATCCCATACATTGGGACAG1200.10833258102374288No Hit
AGCCATGGGATAAAAGACTATTTTTTATCCACTAAATTGACTTTGCATTTTT1200.10833258102374288No Hit
TGCGCGGTTCTTCTAATCCATAAACATGAACTATCTTTCCTTTTTTGTGTCC1200.10833258102374288No Hit
CTCCGAGGGCCAGCTGCCTTTACCAAGGATCAAAGAATCAGAAACTAGGCAG1200.10833258102374288No Hit
TCCATCGTCCCTCATTACTCGGGTTTCATATTTTGCTGTTTTTGATGGACAT1200.10833258102374288No Hit
GCTTATGGGAAGTCACATAGCAGAAGGGAAGCCTCTCCTAACTTAGCTGATC1190.10742980951521171No Hit
TGTCGTGGGGCCCATCTCCATAGCCAGACCAAGCACTCTGGAAGCCAGGGTT1190.10742980951521171No Hit
CGACGGGGGCGCCATGAGTCAATGTGTCCAATGTGACTAGAAACACTGATTG1180.10652703800668052No Hit
CGCTAGAGGGCGAGAAAAGGAACTCTCACCTCGATCTGCCGTGCCGTCCCAA1180.10652703800668052No Hit
TATAATGGGCAAGCCTACGTTTTCACACTTCTAGTAAGCCTCTACCTGCACG1180.10652703800668052No Hit
TGACTAGGGGTGGTAGAATTAAAGGAGGACACGAGCCTGCTTCTGTTACCTC1180.10652703800668052No Hit
GTAGTGGGGTCCACTGGGTTCAATCTGATGTAATGAATATAAAGCATAAGCA1180.10652703800668052No Hit
TTGTCAGGGGTTGATCAGGCTGGTCTTGAACTCCTGACCTTGTGATCCACCC1170.10562426649814932No Hit
GTCACAGGGTCAAAAGAGAATTCAGTCTACAGCCATTTAAATGATCATTGCT1160.10472149498961814No Hit
ATTGCAGGGTCACCCCAAGTTGTAAAAGCTGCAGATACCATAACACCTGAAC1160.10472149498961814No Hit
GACCTCATGATCAACCCTCCTCAGCCTCTCAAAGTGCCGGGATTACAGGCGT1150.10381872348108694No Hit
CGAATAGGGATCGTAGGAAAACATTAAAGAGCAGATGCATGGCCATTTTTCT1150.10381872348108694No Hit
CGATCCGGGGTGTTTTTTTCTTTGTCTGTTTTTAAATACCTGAACTTTTTTC1150.10381872348108694No Hit
AGCTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1150.10381872348108694No Hit
TTAGCAGGGAAAATGATACTATATGTTCATAATTGATGACTTTTAATTCTGG1150.10381872348108694No Hit
CGCTTAGGGACGATCTTGGCTCACTGCAACCTCTGCCTCCCGGGTTCAAGTG1150.10381872348108694No Hit
GATCTTGGGTCTACAGAACAAAATAGGTATGGTTTTTAGAGAAAAGTATATG1150.10381872348108694No Hit
ATATCGGGGAATGTTTAACTTGTTCTGAAAGAAAAACTTATGTCTGTAGGGT1140.10291595197255575No Hit
GCACTGCGGGCCTCCCCTCCTTTGAATTGAAATGGCACATTAAGACTTGTCA1140.10291595197255575No Hit
CAGACCGGGGATGATGTAATTAAAAAATGGTCAGTGTGCAAAGAAAAGAACT1130.10201318046402455No Hit
TCTGTTGGGACATCTAGGAGCTTACTGAAATATGAAAAGGCAAATTTTATAC1130.10201318046402455No Hit
TGAAAAGGGAAACACAAGGAGCCAGGATTGTGCTGGCAGCCAAGGAAACAGT1130.10201318046402455No Hit
TTCCGAGGGAGGTATAAGGTGATATTTCGTTGTGGTTTTAATTTGCATTTCC1120.10111040895549336No Hit
GAGCCGGGGATCACTGGGAGGGGAAGCAGAGAAATGATGAAGAGGATGGAGG1120.10111040895549336No Hit
TAACCAGGGATTCTCCCTGCTACTTTCAGGTACACCAATCAAATGTAGATTT1120.10111040895549336No Hit
TTATTTGGGCTACCTTTGCTTTCCTTTTTCTAAGTTCTTGAGCTTGCAGCTT1120.10111040895549336No Hit
TAATGAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1110.10020763744696218No Hit
GCACCAGGGCTATGAATGTTGGCCTTGTGTGGAAATAGACAAAAATAGATAC1110.10020763744696218No Hit
AGCGAGGGTCAGCAGGTCTGAAAGTAGTTGATGAAAATAATGCAAAAGAACA1110.10020763744696218No Hit
TTAGCCGGGCCTCAGTTTTATCCTGTTAAATGGGAAAGCTTCCCCATCCTCT1110.10020763744696218No Hit
TCAGGTGGGGATGAAGGACTTGGAGGCCCTGATGTTTGATCGCTCCTTTGTG1110.10020763744696218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)