FastQCFastQC Report
Sat 31 Dec 2022
SRR5844447.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844447.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111184
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGCCGTCT7380.6637645704417902RNA PCR Primer, Index 37 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGC6540.5882141315297165No Hit
ATACTAGGGGAATGGGAATAAAGAAAAGCTGAATGACAGGATAAGAGAGGTT1670.15020146783709887No Hit
ACATTAGGGAGGAGATTGTGATCTAGTAAATGTGTCTGGCTCAGTCATTCAG1590.1430061879407109No Hit
GCCAAAGGGATCCAACTGAAGCCCGATGTGGTCATCACTGAAAAGGGCATCT1570.1412073679666139No Hit
CGTTGCGGGATCCCACGTGCCTGCCAATCTGGAAATCAACACTGATGCAACA1510.13581090804432291No Hit
TTCGAGGGGGAAGAAATGCTGCTGGCTCTATTTTTACATTCCCTTCCACCTC1500.13491149805727443No Hit
ATTTAAGGGAACTCCCTCAAGAGGTACGTAGAGGACATTTAAGGGAAGCCTA1490.13401208807022594No Hit
ACAAGAGGGACTGAAAATCTGCTGCCAGATATACTGAAGCTCCATTGTATGT1470.13221326809612896No Hit
GGAACTGGGCAGGAACTGAATTTAGGAAAGGATTGTCCTTGGGGCATTTGTC1460.13131385810908044No Hit
ACTGGTGGGCCACTAAATGATACAGCTGAATGAAGAAAGAATGCTGGAGAAA1460.13131385810908044No Hit
TCAGGTGGGGATGAAGGACTTGGAGGCCCTGATGTTTGATCGCTCCTTTGTG1460.13131385810908044No Hit
ACCACCGGGTTTCAGCAAACATTTATGAAGCTCCTGTAATATTCAAACCTCC1450.13041444812203193No Hit
GATCAAGGGAAATATTGTGGGTGTTGCATAAATTGCAGAGATATTTGTATTA1440.12951503813498344No Hit
CTCCTAGGGACAGTGCAGCTCCACATTAACTCTACAGACCAAACCATTTGTA1440.12951503813498344No Hit
TCAATCGGGAGTGTCAGGACTCTGCCAGTTACTTTCAGATGTTTCAGGGGAA1440.12951503813498344No Hit
ATTGCAGGGTCACCCCAAGTTGTAAAAGCTGCAGATACCATAACACCTGAAC1410.12681680817383795No Hit
GCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATA1400.12591739818678946No Hit
GACAAAGGGATGAAATCAGATTGCCAACTTCTTGACTTTCAATGTTAGACAT1400.12591739818678946No Hit
CACAATGGGTCAATTCAACAAAAAAACCTAACTATCTTAAATATATATGCAC1400.12591739818678946No Hit
TAGCTAGGGGTAATAATAAAAATGATTTATCCAATTAGAAAAGGACAAGATA1400.12591739818678946No Hit
CACACAGGGCTGAGGAAAACTGCAATGAAAATGGATCTGATTTACTCATCTT1380.12411857821269248No Hit
AGCCATGGGATAAAAGACTATTTTTTATCCACTAAATTGACTTTGCATTTTT1380.12411857821269248No Hit
TGCTCGGGAAGGTCACTGATCAGTGCCTGTCCTTCTTCAAACGCTGTGGAAA1370.12321916822564398No Hit
TCAGCTGGGAGAAGGTAGACAGCTGTAACCTCTAACATTGATACTTCGATCT1370.12321916822564398No Hit
AATGCCGGGATTAGGTCAGCATGTGAAATGTTGGATTTGACATGTCAGGTAG1340.1205209382644985No Hit
AGTACAGGGGATAACGGCGGCGAGCGGACGGCTGCATTTACGGGGTCTCCCG1340.1205209382644985No Hit
GCAGTGGGGAGATTATCACCAGTACTCACATTGTGCATTCTAAAAGCGATTT1330.11962152827745No Hit
CTTGTTGGGATCCCTGGTGCAAAATTACTAAGAGTGAATAGAAGGCTAGAAA1320.11872211829040148No Hit
TGAAAAGGGAAACACAAGGAGCCAGGATTGTGCTGGCAGCCAAGGAAACAGT1310.117822708303353No Hit
CGCTAGAGGGCGAGAAAAGGAACTCTCACCTCGATCTGCCGTGCCGTCCCAA1300.11692329831630449No Hit
TATAATGGGCAAGCCTACGTTTTCACACTTCTAGTAAGCCTCTACCTGCACG1300.11692329831630449No Hit
TGTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1300.11692329831630449No Hit
CGGGGCGGGATCAGGAATTGCCACTACTGATTAGCAATAATATTTAACAAGG1300.11692329831630449No Hit
CACGGAGGGGAGCCGGGGAGGTGATCTGTATAGACGACTGACTGGATCCCAG1290.116023888329256No Hit
CGAATAGGGATCGTAGGAAAACATTAAAGAGCAGATGCATGGCCATTTTTCT1290.116023888329256No Hit
GGCGTAGGGGATGACACCACTTTCTGGGAACTTGAGGCAAGGTAGGTGGCAT1290.116023888329256No Hit
AAAATAGGGCAACATCCTGAATGTTAGAAGCCTTCATACACTTGTGAAATTG1290.116023888329256No Hit
CTCCGAGGGCCAGCTGCCTTTACCAAGGATCAAAGAATCAGAAACTAGGCAG1290.116023888329256No Hit
GCACCAGGGCTATGAATGTTGGCCTTGTGTGGAAATAGACAAAAATAGATAC1280.11512447834220751No Hit
GTCTGGGGATAGATTGAAAATGCATTATTTACCTCTCTTTATTTTCATCTTC1280.11512447834220751No Hit
TTTGTAGGGCTACTGATGGTGGCCATCATGCTGGGTGTCTGCTCCATCATGG1270.11422506835515901No Hit
AGCGAGGGTCAGCAGGTCTGAAAGTAGTTGATGAAAATAATGCAAAAGAACA1270.11422506835515901No Hit
TAAGTGGGAGTGTCGGCAGGGAAATCTAGAACTCTCATATACCATTTGGTTA1260.11332565836811052No Hit
TTGGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1260.11332565836811052No Hit
TTTAGAGGGTCTCTCAAACTATGGAAGTTGCATTGACATCTTCTCTGAATGC1260.11332565836811052No Hit
AGCCTTGGGAACCAAAGCCATCTTGAGCAAAAAAACAAAGCTGGAGGTGTTC1250.11242624838106202No Hit
TCGACAGGGATCCCTAAAGATGGGTTAATCCTGGCTGTTCATTCTCAGGAAA1250.11242624838106202No Hit
AGTAATGGGAGCAGTCTGCCTCCTCTTTCAACATGACAGATGCCGCTGTGTC1250.11242624838106202No Hit
TTTGTTGGGACTATAACATCACTTGATGGCGGATTGTGGTAAGTTTAAAATG1240.11152683839401353No Hit
CAACGCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1230.11062742840696503No Hit
AGCTCAGGTCCACAGATGCATTACGAAAAATACAGGAAAGCTATGGAGATGT1230.11062742840696503No Hit
AACTAAGGGTTGTGACTACTTGTTGAAATGTGAGTAAAAGTACTTTGTGGCA1230.11062742840696503No Hit
GTGTAATGGGATCTCCGTCCGCCTTCTCTCCTACCTAAGTGCGTGCCGCCAC1220.10972801841991654No Hit
TTTATTGGGAGATTCCTTTGTTGGACTAATGGGCAAAAGAGCTTTAAATTCT1210.10882860843286805No Hit
TGACCTGGGGATTTTTCTTTCACATTGCAAGTGGGAGAGCAAAAGTGTTTTA1210.10882860843286805No Hit
GCGATGGGGACTGTTAGCAGACCTACTGCTATAAACTTTCTTCCTTTGGCTT1210.10882860843286805No Hit
GTCACAGGGTCAAAAGAGAATTCAGTCTACAGCCATTTAAATGATCATTGCT1200.10792919844581954No Hit
TTACCTGGGGGATCTCGATATTTCAGATGGGCTTTTGATGCACTGTTGCCAA1190.10702978845877104No Hit
TTAGTAGGAGTGAGCTATGATCATGCCATTGTCTTCCAGCCTGAGTGTCAGA1190.10702978845877104No Hit
ATTGAAGGGCAAGACTGTCTTTATTTTACCGGATTGGAGCTTGCCAGGTGTG1190.10702978845877104No Hit
CGGCAAGGGGTGTACATGTCATATAAAAATAAATACTTTTTAAAATAATTTT1180.10613037847172255No Hit
TAATCAGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG1180.10613037847172255No Hit
GAGCCGGGGATCACTGGGAGGGGAAGCAGAGAAATGATGAAGAGGATGGAGG1170.10523096848467405No Hit
AGCTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1170.10523096848467405No Hit
CTTAGCGGGTTGTTGGATCTTAGGATAAAGTTGGTTGTGTCCAGAGGTGACT1170.10523096848467405No Hit
AATCTAGGGATCTATATATCAGCTATACCATTATATGCATACTATTTTTCTT1170.10523096848467405No Hit
AGAATAGGGACCCCAAAATATAGGTTGAATTTGGTATTGATCCCTGGCCTTC1170.10523096848467405No Hit
ATAGCAGGGAGAGGTCCTTCACGTCCCTTGTAAGTTGGATTCCTAAGTATTT1160.10433155849762556No Hit
CAATCCGGGTGGCCCAATAGCCTTTGCTTGCAAGCTCATCTGTAAATTTAAA1160.10433155849762556No Hit
GCACTGCGGGCCTCCCCTCCTTTGAATTGAAATGGCACATTAAGACTTGTCA1150.10343214851057705No Hit
TCTGTTGGGACATCTAGGAGCTTACTGAAATATGAAAAGGCAAATTTTATAC1150.10343214851057705No Hit
TGTCGTGGGGCCCATCTCCATAGCCAGACCAAGCACTCTGGAAGCCAGGGTT1150.10343214851057705No Hit
CGCACCGGTTCAACAACAAATTTTGGAACATAAAAAGAAGATACTTAAAAAG1150.10343214851057705No Hit
TGGCCGGGATAAATGTCAATTCTGAAAAGTACAAATCTCTTGGAAGCTGAAA1140.10253273852352857No Hit
GATAAAGGGGGAATAGTCAATTCAGAAATGGTGAGTGACAGATGTGGTTTGG1140.10253273852352857No Hit
AAGGCTGGGGCTGCCTGGGCTACACGCTGGTGAGGGAATTCAGCAAAGACCT1140.10253273852352857No Hit
GATCTTGGGTCTACAGAACAAAATAGGTATGGTTTTTAGAGAAAAGTATATG1140.10253273852352857No Hit
TCAGCAGGGGCTGGCTTTTAACATGTATACTAGCAAACCTATTTATGAGAGC1130.10163332853648008No Hit
TATTATGGGAACACTCTCTTCCTGTAGATAGTTTGATGGCTCAAATATTCCC1130.10163332853648008No Hit
TCACGGGGGAACCAATTTATACTCCTGTCAGCAATTTATTATAGTATCCATT1130.10163332853648008No Hit
GGCATAGGGGTTAGATGACATTGTCTTCATTAAGATCTTCATGTCAAGCCTG1130.10163332853648008No Hit
TGTGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1120.10073391854943158No Hit
CGATCCGGGGTGTTTTTTTCTTTGTCTGTTTTTAAATACCTGAACTTTTTTC1120.10073391854943158No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)