FastQCFastQC Report
Sat 31 Dec 2022
SRR5844446.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844446.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108330
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGCCGTCT7050.6507892550540016RNA PCR Primer, Index 37 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGC5900.5446321425274624No Hit
GGAACTGGGCAGGAACTGAATTTAGGAAAGGATTGTCCTTGGGGCATTTGTC1930.17815932797932243No Hit
ACATTAGGGAGGAGATTGTGATCTAGTAAATGTGTCTGGCTCAGTCATTCAG1750.16154343210560324No Hit
ATACTAGGGGAATGGGAATAAAGAAAAGCTGAATGACAGGATAAGAGAGGTT1620.14954306286347271No Hit
ACCACCGGGTTTCAGCAAACATTTATGAAGCTCCTGTAATATTCAAACCTCC1590.14677374688451952No Hit
ACTGGTGGGCCACTAAATGATACAGCTGAATGAAGAAAGAATGCTGGAGAAA1460.134773377642389No Hit
AGGTCTGGGATATAAGAACATCTGTTTCACAGTCTGGGACGTGGGAGGCCAG1390.12831164035816486No Hit
AACTAAGGGTTGTGACTACTTGTTGAAATGTGAGTAAAAGTACTTTGTGGCA1390.12831164035816486No Hit
TGAAAAGGGAAACACAAGGAGCCAGGATTGTGCTGGCAGCCAAGGAAACAGT1390.12831164035816486No Hit
ATAGCAGGGCCGTCCAATGCCTGCATGCTGCTTGAATTGCTCCACCCACACC1350.12461921905289393No Hit
AGCTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1350.12461921905289393No Hit
AATGCCGGGATTAGGTCAGCATGTGAAATGTTGGATTTGACATGTCAGGTAG1350.12461921905289393No Hit
CAATCCGGGTGGCCCAATAGCCTTTGCTTGCAAGCTCATCTGTAAATTTAAA1340.12369611372657621No Hit
TTGGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1320.12184990307394074No Hit
GACAAAGGGATGAAATCAGATTGCCAACTTCTTGACTTTCAATGTTAGACAT1320.12184990307394074No Hit
CGACGGGGGCGCCATGAGTCAATGTGTCCAATGTGACTAGAAACACTGATTG1310.120926797747623No Hit
TCCATCGTCCCTCATTACTCGGGTTTCATATTTTGCTGTTTTTGATGGACAT1290.11908058709498755No Hit
CACAATGGGTCAATTCAACAAAAAAACCTAACTATCTTAAATATATATGCAC1280.1181574817686698No Hit
AGTAAAGGGGTGCCTTTGGGGCAGACTCCAGACAAGGATGTCTGAGACCACT1270.11723437644235207No Hit
CTTGTTGGGATCCCTGGTGCAAAATTACTAAGAGTGAATAGAAGGCTAGAAA1260.11631127111603433No Hit
CTCCTAGGGACAGTGCAGCTCCACATTAACTCTACAGACCAAACCATTTGTA1250.1153881657897166No Hit
TTTATTGGGAGATTCCTTTGTTGGACTAATGGGCAAAAGAGCTTTAAATTCT1230.11354195513708114No Hit
TGTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1220.11261884981076341No Hit
TCAATCGGGAGTGTCAGGACTCTGCCAGTTACTTTCAGATGTTTCAGGGGAA1220.11261884981076341No Hit
GTCACAGGGTCAAAAGAGAATTCAGTCTACAGCCATTTAAATGATCATTGCT1210.11169574448444566No Hit
TTTGTAGGGCTACTGATGGTGGCCATCATGCTGGGTGTCTGCTCCATCATGG1210.11169574448444566No Hit
GCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATA1200.11077263915812793No Hit
ACTGATGGGATTGTTTAGACAAAATCATAATCTCAGTGTATTTTTTCTTGGG1200.11077263915812793No Hit
CAACGCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1200.11077263915812793No Hit
TAGACAGGGCAATGGCAGCGACGGACACAAGAGGAAAGAGGAATTCTTCATC1200.11077263915812793No Hit
ACAAGAGGGACTGAAAATCTGCTGCCAGATATACTGAAGCTCCATTGTATGT1200.11077263915812793No Hit
GATCTTGGGTCTACAGAACAAAATAGGTATGGTTTTTAGAGAAAAGTATATG1200.11077263915812793No Hit
AAAATAGGGCAACATCCTGAATGTTAGAAGCCTTCATACACTTGTGAAATTG1190.10984953383181022No Hit
CTAGGCGGGTAAATCACCTCCTTGCAACCCCCAAATGACTTTGGAATAACTG1190.10984953383181022No Hit
TCTGTTGGGACATCTAGGAGCTTACTGAAATATGAAAAGGCAAATTTTATAC1190.10984953383181022No Hit
ATTGAAGGGTGACATACTATAGGTTATGTTTTAAAACGACATCCAAATGCTA1180.10892642850549247No Hit
TGTTTTGGGAATTTGGGATTATTTCAACTTTACATTGTATGTCTGAATTTCA1180.10892642850549247No Hit
CGTTGCGGGATCCCACGTGCCTGCCAATCTGGAAATCAACACTGATGCAACA1180.10892642850549247No Hit
GTCTGGGGATAGATTGAAAATGCATTATTTACCTCTCTTTATTTTCATCTTC1180.10892642850549247No Hit
TTAGCAGGGAAAATGATACTATATGTTCATAATTGATGACTTTTAATTCTGG1180.10892642850549247No Hit
TCTTAAGGGATTACTTTAATGCTTTAAGCCATAGTTGTCCAATCTGTCTTTC1170.10800332317917474No Hit
TTCGAGGGGGAAGAAATGCTGCTGGCTCTATTTTTACATTCCCTTCCACCTC1170.10800332317917474No Hit
TAATGAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1160.107080217852857No Hit
CGGGGCGGGATCAGGAATTGCCACTACTGATTAGCAATAATATTTAACAAGG1160.107080217852857No Hit
TCTCCGGGGAAAGTGTCTCCTAAAATGGTGGGTGAAGGAGATTATTAATGAT1150.10615711252653928No Hit
TCGACAGGGATCCCTAAAGATGGGTTAATCCTGGCTGTTCATTCTCAGGAAA1150.10615711252653928No Hit
ATTTAAGGGAACTCCCTCAAGAGGTACGTAGAGGACATTTAAGGGAAGCCTA1150.10615711252653928No Hit
AGTGTAGGGCTCAGCGGATGTGGCAGCCCCGAGCCATGGCTCTCGCCGTCCG1140.10523400720022155No Hit
AGTACAGGGGATAACGGCGGCGAGCGGACGGCTGCATTTACGGGGTCTCCCG1140.10523400720022155No Hit
TGCGCGGTTCTTCTAATCCATAAACATGAACTATCTTTCCTTTTTTGTGTCC1140.10523400720022155No Hit
CGTTGTGGGGTCCCATGGTGTTATTCAAGGATTACAGTATTACAATAAACAC1140.10523400720022155No Hit
AGCCTTGGGAACCAAAGCCATCTTGAGCAAAAAAACAAAGCTGGAGGTGTTC1130.10431090187390381No Hit
TTTGTTGGGACTATAACATCACTTGATGGCGGATTGTGGTAAGTTTAAAATG1130.10431090187390381No Hit
AAGTATGGGAGCCAGAGGTGGAGCCTCAGGTGGACAGCCCTGCTCCTCCAAG1120.10338779654758608No Hit
CGATCCGGGGTGTTTTTTTCTTTGTCTGTTTTTAAATACCTGAACTTTTTTC1120.10338779654758608No Hit
CATCATGGGTGTTAGTGGAGACCGCTAAACTAATGATGTTTGAAAACAGTTC1120.10338779654758608No Hit
TTTAGAGGGTCTCTCAAACTATGGAAGTTGCATTGACATCTTCTCTGAATGC1120.10338779654758608No Hit
TCGCAGGGAATTCCGCTCTCTTGAGAAATCGGCTCTGTCCAGGCAGCAGCAA1110.10246469122126833No Hit
GCACCAGGGCTATGAATGTTGGCCTTGTGTGGAAATAGACAAAAATAGATAC1110.10246469122126833No Hit
AAATAAGGGACCCTCCTGCCTATTCAGGATGTGGATGCCACAGGAGAGCAGC1110.10246469122126833No Hit
AGCAATGGGCTATGCATTAATGAAAATGATCCTGAGTGGAGGTTAGCTGAAC1110.10246469122126833No Hit
CGCTAGAGGGCGAGAAAAGGAACTCTCACCTCGATCTGCCGTGCCGTCCCAA1100.10154158589495062No Hit
GCACTGCGGGCCTCCCCTCCTTTGAATTGAAATGGCACATTAAGACTTGTCA1100.10154158589495062No Hit
TATAATGGGCAAGCCTACGTTTTCACACTTCTAGTAAGCCTCTACCTGCACG1100.10154158589495062No Hit
AAGCCCGGAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT1100.10154158589495062No Hit
TCAGATGGGGGAAACGTAATGATCTTGGCAAGCTGTTAATGTCTGTCCCATC1100.10154158589495062No Hit
CACACAGGGCTGAGGAAAACTGCAATGAAAATGGATCTGATTTACTCATCTT1100.10154158589495062No Hit
ACGATAGGGAACAAATCCTAAAAAATAGGAAACTATCGTCTCCATTTTACAT1100.10154158589495062No Hit
TTGTCAGGGGTTGATCAGGCTGGTCTTGAACTCCTGACCTTGTGATCCACCC1100.10154158589495062No Hit
CAGACCGGGGATGATGTAATTAAAAAATGGTCAGTGTGCAAAGAAAAGAACT1100.10154158589495062No Hit
TTTCACGGGGTATGAAGATGAAATCAGCGATTCCAAAGCACAGCTAGCTGCC1090.10061848056863289No Hit
ATCTAAGGGGCATTTTTCTATTTCAATGTTACCTGAATATCACAGAGGTTAA1090.10061848056863289No Hit
GCGATGGGGACTGTTAGCAGACCTACTGCTATAAACTTTCTTCCTTTGGCTT1090.10061848056863289No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)