FastQCFastQC Report
Sat 31 Dec 2022
SRR5844414.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844414.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270364
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGC7550.27925315500584397No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGGAATATCTCGTATGCCGTCT6900.25521149265434745RNA PCR Primer, Index 37 (95% over 22bp)
CATCTAGGGAGCAGGTGCAACAAAAACAAGAAACAAATGTGCCCACCCCACT2900.10726280126052284No Hit
GAGCCCGGGAATTGGAATTTAAAATGAAAAACACAATACTATTTACATTAGC2750.10171472533325443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)