FastQCFastQC Report
Sat 31 Dec 2022
SRR5844400.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844400.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146054
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGC6980.4779054322373916No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGCCGTCT6450.44161748394429456Illumina PCR Primer Index 1 (95% over 23bp)
AATGTTGGGGATCTAGAGAGGGGTTGGAGAAAATAATGGAAGAGAGAGATAT3910.2677092034453011No Hit
TTATGTGGGGAGGGGCTCGTGTGCTGGGCGACAGTGACACACTCAGCAGCCC2380.16295342818409628No Hit
CTATTAGGGATCTCATTGTGGCTTTAATTTGCATTTCTCTGATGATTAGTGA2220.15199857586919907No Hit
AATTATGGGGCCTAGGCCCTGGTGAAACCACAGGTATGCAATTATGCTAGGG2120.1451517931723883No Hit
CTAGACGGGAAGTAACACATTTGGCCACAAAACACCAAAGAATTGTAGGCAG2110.14446711490270722No Hit
GTCTCGGGGAACCCGCCACATCTACCATCACCCTCTACATCACCGCCCCGAC2090.14309775836334504No Hit
TTAAAGGGAAGAGAAAGGAGGAAAGATCTAAAATCGACACCCCAACATCACA2040.13967436701493968No Hit
GCGTGGGGGCTGCCAGCTGCGAGCCCCTGGCCTCCGTGCTCAGGGCCAAGCC1920.13145822777876676No Hit
ATGCGAGGGGTCTTCAAACTGAAAATTTAGAATGAACATTTCAAAGTAGGAA1910.13077354950908568No Hit
TTTTCAGGGGAAATTGATCAGCAGGCAAAATGCCCTTGAGGAGCTTATCTTC1900.1300888712394046No Hit
TGCTAAGGGAGATATGGTGACATTCAGCTGGCATGTGGAAATGAATTATTGT1880.12871951470004245No Hit
ACGATAGGGCTGAACTCAGGGCACGACTAGAAGCCCTCAAAACCCCTGGGAC1850.12666547989099922No Hit
CAGTGCGGGAGGAATATACACACATAAGTCAAAAGAAGACATGTCTACAGAG1830.12529612335163706No Hit
TGTATGGGGTGGAGTCAGTTACGTTGTTAAGGATGCCTTTGGCTACAAGTAA1830.12529612335163706No Hit
TCGGGGACCAGCCACTGCAAAAACAAACCAAAATATAAAGACCAATGAGACT1820.12461144508195598No Hit
AACAATGGGAGAGGCCCCGGGGAGCTGTCTTGCCCTATTCTACTGTGGGTGG1810.12392676681227491No Hit
GCTTATGGGATCCCCAGTGTGCCCCCGGCCCCGGGTCCGCCTTGGCTCGGAG1770.1211880537335506No Hit
TCGACGGGGGTGTTGATGTGTGTCCTGCAACCTGGGGCATCAACATCCTAAT1750.11981869719418846No Hit
GCACCAGGAGAGGTTGCAGTGAGCCGAAAGTGCGCCACTGCGCTCCAGCCTG1740.11913401892450738No Hit
TCGCCAGGGATGGGCCAAACAGGTCCCATCCTCTTCATGACAGCTGTGAGCT1720.11776466238514521No Hit
TCTTTAGGGCAAAGCTGCAGAGTGAAGATAATATTGGACTAATAATTGGATT1710.11707998411546415No Hit
TGTGCTGGGATCCTCAATCTTGCTGCAGTGTCGGGCAGTCCTCCTATTGGCA1700.11639530584578307No Hit
TGACCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1690.115710627576102No Hit
AATTGTGGGGGCGCCGGAAGTGGAAGACCAGGCAGCCCAGCTGAAGGCAGTA1690.115710627576102No Hit
CCAGGCGGGCATCCCGGGTGCGATTTGGTCAACAAAAGCGATACCAAGATTG1680.1150259493064209No Hit
GTTGCCGGGTAGAAAATTTAGGTAGAAAATACTCTGAATAATAAAAAAGTAA1680.1150259493064209No Hit
GCTGGGGTGCAACAAAGATGCTGGGAAGTGGTTAAGTGTGCCCACCCGTGTT1670.11434127103673983No Hit
TATCTGGGGGGCGGCCGGCCAGTTCCAGCCCGCACCCCGCGTCGGTGCCCGC1660.11365659276705876No Hit
GGAGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1630.11160255795801552No Hit
CACACTGGGATATTAGTCTTTGGAAGTTTAGCTGCCTGGCCTCTTTCACTTA1630.11160255795801552No Hit
GCGTATGGGATAGATATTTCTCAAAAAAAGACAAACAAATGGCAAACAGGCA1630.11160255795801552No Hit
CAGCCTGGGCAACAAGACCGAAACTCCGTCTCAGATAAAAAACAAACAAACA1620.11091787968833444No Hit
TGGCATGGGATTTCTGCAGAACTTCTGTTGATGTACAGCATTGTATTTAGCA1620.11091787968833444No Hit
ACGTTGGGCATCCGGGCCCGGGTGTCTTCCGGCAGCTCCTCTGAGGTCTCCT1590.10886384487929122No Hit
AATTGGGGGGGAATTAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGG1580.10817916660961013No Hit
ACGATGGGGAGGTCCGAGGGGGCGGGGGCAGGGCCAAGAGTTCAAAAGAAGC1570.10749448833992908No Hit
TGCTATGGGATATAATGGTTTGCAGGAACAAAAGTATAATGTGACTTGCAAG1570.10749448833992908No Hit
TCACTGGGCTAGCTCTGGGACTGGTGATGAGATCGGGAAAGTGGGCTCAGGA1570.10749448833992908No Hit
TAACTTGGGGGTTCTGTGTTGTGTGCCGAGTGCCGCCCAGTGTTCTGTGACC1570.10749448833992908No Hit
AACACAGGGAGCATGAGGTATTCTAAGGTCTATTGTTCAACGAATAAGATAT1560.10680981007024799No Hit
GTATTTGGGGAGGGAAAAATGGGAACTATCAGCATAGAAATGGAAATTAAAA1560.10680981007024799No Hit
CAGCTGGGTAAAGCGGGAGGTAGACATGTAGCAGTAAAAATAGTTAAAAATG1540.10544045353088584No Hit
ATTGTTGGGAGTACAGTCCTTGCATCCATTCTATTTTTAACTTCCATATCTG1540.10544045353088584No Hit
TGTGCAGGGATGTACCCTTTACTGCTCTAAAATTGACCACATAATTGGACAT1530.10475577526120475No Hit
GCGCTAGGGATGGAATCCTTACCTCAGTAAACCACCGAAGCCCTCTTCAAGC1530.10475577526120475No Hit
TACCTTGGGAACTTTTCTGGGTCTCAGGCTCTGATGTTTGAAAAAGAAGGGA1530.10475577526120475No Hit
AGCCGGGGCTCACTGTTAAGTGCCCAAGGAATGAACATGTAATAATTTGTTT1530.10475577526120475No Hit
TCAGTGAGGCATCAAGATCTGGAAGGCAAAAACAAGGATAAAATTTCCCCAA1530.10475577526120475No Hit
ACGCAGGGGGATCTCTCTCGGGGTACACAGTTGGAATTCTGTATTCAGTGAT1520.1040710969915237No Hit
CAGCATGGGAAGTTAAGCATATGCTTACTGCAAGAAGTCACAACAAAAGTTT1520.1040710969915237No Hit
TATTGGGGGTCACCAGCGCCAGCACAACAAGGACAAGCCCTACAAGTGTCCC1520.1040710969915237No Hit
CATATAGGGCAGCAGCTTGCACAAAATCCTTACAGCTTGGGTGGTTCCCTGG1510.10338641872184261No Hit
GCAATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1510.10338641872184261No Hit
GCGAGGGGAAATCAGCAGAGGTGGAATTGAAGACAGGAGGAAATAATTCAAA1510.10338641872184261No Hit
CGGTCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1510.10338641872184261No Hit
CACAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1510.10338641872184261No Hit
TCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG1500.10270174045216153No Hit
TCGTAAGGGGCTCTCAGATTGTTTAGCTTGTTTGACAGCTCTGTAGACTTAT1500.10270174045216153No Hit
TACGCTGGGGTTCTCCCCAGCTTTGGGCCTTCCCTTCATCAGCTGCATTGCC1500.10270174045216153No Hit
GCTAGAGGGGGAGGAAAGAAATGGAAATTCAAATTCAAGAAGCTTAAACAAG1490.10201706218248045No Hit
TGCGTGGGGAACTTTTTGCCTCCATAAAGTTGTTAATTTATTATTTTATCTG1490.10201706218248045No Hit
TATACTGGGTAAGACACAGAGCTGCTTATCATCCAGACTTTCCAACAGTTCT1480.10133238391279936No Hit
AGCTAGGGACGAGGCCCCTCTCCCAATGCCTTTCCCAAATCTCTCCTTATGG1480.10133238391279936No Hit
GTGGCAGGGCAAGGGTTAAAGGGGTGACATTGAGGGATGCCTCAGAGTCAAA1470.10064770564311831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)