FastQCFastQC Report
Sat 31 Dec 2022
SRR5844399.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844399.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144814
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGC6250.4315881061223362No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGCCGTCT6150.42468269642437884Illumina PCR Primer Index 1 (95% over 23bp)
AATGTTGGGGATCTAGAGAGGGGTTGGAGAAAATAATGGAAGAGAGAGATAT3400.23478392973055093No Hit
TTATGTGGGGAGGGGCTCGTGTGCTGGGCGACAGTGACACACTCAGCAGCCC2730.18851768475423647No Hit
TCGGGGACCAGCCACTGCAAAAACAAACCAAAATATAAAGACCAATGAGACT2230.1539906362644496No Hit
AATTATGGGGCCTAGGCCCTGGTGAAACCACAGGTATGCAATTATGCTAGGG2190.15122847238526663No Hit
CTATTAGGGATCTCATTGTGGCTTTAATTTGCATTTCTCTGATGATTAGTGA2150.14846630850608367No Hit
GCGTGGGGGCTGCCAGCTGCGAGCCCCTGGCCTCCGTGCTCAGGGCCAAGCC2100.14501360365710497No Hit
CCAGGCGGGCATCCCGGGTGCGATTTGGTCAACAAAAGCGATACCAAGATTG2060.14225143977792204No Hit
TCGACGGGGGTGTTGATGTGTGTCCTGCAACCTGGGGCATCAACATCCTAAT1900.13120278426119023No Hit
ATGCGAGGGGTCTTCAAACTGAAAATTTAGAATGAACATTTCAAAGTAGGAA1880.12982170232159876No Hit
TGACCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1860.12844062038200726No Hit
TTTTCAGGGGAAATTGATCAGCAGGCAAAATGCCCTTGAGGAGCTTATCTTC1860.12844062038200726No Hit
GGAGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1810.12498791553302857No Hit
CAGCTGGGTAAAGCGGGAGGTAGACATGTAGCAGTAAAAATAGTTAAAAATG1800.12429737456323284No Hit
ACGTTGGGCATCCGGGCCCGGGTGTCTTCCGGCAGCTCCTCTGAGGTCTCCT1790.12360683359343709No Hit
TAACTTGGGGGTTCTGTGTTGTGTGCCGAGTGCCGCCCAGTGTTCTGTGACC1770.12222575165384561No Hit
GCACCAGGAGAGGTTGCAGTGAGCCGAAAGTGCGCCACTGCGCTCCAGCCTG1760.12153521068404988No Hit
CTAGACGGGAAGTAACACATTTGGCCACAAAACACCAAAGAATTGTAGGCAG1750.12084466971425416No Hit
ACGATAGGGCTGAACTCAGGGCACGACTAGAAGCCCTCAAAACCCCTGGGAC1730.11946358777466268No Hit
TCAGTGAGGCATCAAGATCTGGAAGGCAAAAACAAGGATAAAATTTCCCCAA1730.11946358777466268No Hit
AGCCGGGGCTCACTGTTAAGTGCCCAAGGAATGAACATGTAATAATTTGTTT1710.1180825058350712No Hit
TCTTTAGGGCAAAGCTGCAGAGTGAAGATAATATTGGACTAATAATTGGATT1710.1180825058350712No Hit
TGCTAAGGGAGATATGGTGACATTCAGCTGGCATGTGGAAATGAATTATTGT1700.11739196486527546No Hit
TGTATGGGGTGGAGTCAGTTACGTTGTTAAGGATGCCTTTGGCTACAAGTAA1700.11739196486527546No Hit
CAGCCTGGGCAACAAGACCGAAACTCCGTCTCAGATAAAAAACAAACAAACA1680.11601088292568398No Hit
CAGTGCGGGAGGAATATACACACATAAGTCAAAAGAAGACATGTCTACAGAG1680.11601088292568398No Hit
TACCTTGGGAACTTTTCTGGGTCTCAGGCTCTGATGTTTGAAAAAGAAGGGA1680.11601088292568398No Hit
GCTTATGGGATCCCCAGTGTGCCCCCGGCCCCGGGTCCGCCTTGGCTCGGAG1670.11532034195588824No Hit
TGCTATGGGATATAATGGTTTGCAGGAACAAAAGTATAATGTGACTTGCAAG1670.11532034195588824No Hit
TATCTGGGGGGCGGCCGGCCAGTTCCAGCCCGCACCCCGCGTCGGTGCCCGC1670.11532034195588824No Hit
GTCTCGGGGAACCCGCCACATCTACCATCACCCTCTACATCACCGCCCCGAC1650.11393926001629677No Hit
TTAAAGGGAAGAGAAAGGAGGAAAGATCTAAAATCGACACCCCAACATCACA1650.11393926001629677No Hit
TCACTGGGCTAGCTCTGGGACTGGTGATGAGATCGGGAAAGTGGGCTCAGGA1650.11393926001629677No Hit
TGATGGGGGGCGAGAGTTAGCGGCCTCCGGTGTGGGATGGCCGCGGAGCCGG1640.11324871904650102No Hit
CACAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1640.11324871904650102No Hit
TCTGTGGGGGGAACCCAGAAACAGGAGAACAAGTAGCCATGTATTCTGACTT1640.11324871904650102No Hit
GTGGCAGGGCAAGGGTTAAAGGGGTGACATTGAGGGATGCCTCAGAGTCAAA1620.11186763710690954No Hit
GCGTATGGGATAGATATTTCTCAAAAAAAGACAAACAAATGGCAAACAGGCA1620.11186763710690954No Hit
TCGCCAGGGATGGGCCAAACAGGTCCCATCCTCTTCATGACAGCTGTGAGCT1610.11117709613711381No Hit
GAACTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1610.11117709613711381No Hit
TGTCTCGGGCACTTCGAAAAGTTATTTAATCTTCTAATGTTCAATAATTTCA1590.10979601419752233No Hit
GTTGCCGGGTAGAAAATTTAGGTAGAAAATACTCTGAATAATAAAAAAGTAA1580.10910547322772661No Hit
CAGCATGGGAAGTTAAGCATATGCTTACTGCAAGAAGTCACAACAAAAGTTT1580.10910547322772661No Hit
ACGCAGGGGGATCTCTCTCGGGGTACACAGTTGGAATTCTGTATTCAGTGAT1570.10841493225793088No Hit
AATTGGGGGGGAATTAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGG1570.10841493225793088No Hit
CGGTCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1560.10772439128813513No Hit
TTTAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1560.10772439128813513No Hit
AATTGTGGGGGCGCCGGAAGTGGAAGACCAGGCAGCCCAGCTGAAGGCAGTA1560.10772439128813513No Hit
GTAACAGGGATGCTATTTTGCTCTTCCCATCAAAATAAAGAAACTTCCAGAG1550.1070338503183394No Hit
TGTGCAGGGATGTACCCTTTACTGCTCTAAAATTGACCACATAATTGGACAT1550.1070338503183394No Hit
GGATCAGGGATAGAGAACAATTTTGAAGTTCCTCAAAAAACTAAAAACTGAG1540.10634330934854365No Hit
CACACTGGGATATTAGTCTTTGGAAGTTTAGCTGCCTGGCCTCTTTCACTTA1530.10565276837874792No Hit
ATATAAGGGCAGTGGTTGGCTTGTAAGCTCTGGCTCCAAGTGTTCTGAGGTG1530.10565276837874792No Hit
GCAGCAGGGCTCTCCTCCCTTATCACCTAGTGTTGCTTAAAGAGGAGGCCAT1520.10496222740895217No Hit
GCGAGGGGAAATCAGCAGAGGTGGAATTGAAGACAGGAGGAAATAATTCAAA1520.10496222740895217No Hit
TGTGCCGGGGTGTTACTAAAGTGTCTAGTTTTTTGAAATGCAAAGCTTTATG1520.10496222740895217No Hit
CACGCAGGGTCCCAGAGCAGAGTGGACCAGACATAGCCATGCACATGCGAAA1520.10496222740895217No Hit
AACTACGGGTTACTCTGCTTGTCTTTATGTTGCTGGAACATTTCTAAAAGAC1520.10496222740895217No Hit
CTGTCTGGGAGATCAACTTCTGTTATGGTGCTATGCTTGGATAAGAAGAGAA1520.10496222740895217No Hit
GGGGCAGGGGAACTCATTTAAAGAAAGAAGGGTAAACTTCTAGAAGTGAGAT1510.10427168643915644No Hit
CTCTATGGGATAGAGTTCTGACCTTCACCTGCTTCCTTCTGTTTCTGTGGCT1500.1035811454693607No Hit
TTATAAGGGCGGGGACACCAGGGGCTTCCTGATTGACGGCTATCCTCGGGAG1500.1035811454693607No Hit
TATTGCGGGGAAGAGATTAACAGGTTTACCAAAGATTATTTTTATAACCTCT1490.10289060449956496No Hit
CGTGGCGGGTGCAGGTGGGCATTTATGTGGCAGAAGTTCTGTTCCCTATAAT1490.10289060449956496No Hit
AATTGTGGGTACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGAC1490.10289060449956496No Hit
GTATTTGGGGAGGGAAAAATGGGAACTATCAGCATAGAAATGGAAATTAAAA1490.10289060449956496No Hit
TCCTAGGGGATGCCCCGTATTTTTGTTTACTTCTCCAAATCCACTATGCACC1490.10289060449956496No Hit
GCGCATGGGGAGGTGAAGGGCCGCTGTGGCCACAGCCAGTCGGAGCTGCAAG1480.10220006352976922No Hit
TGTCAGGGGATAGCGTGGATTCCCCGGAACAGCTGTCTGGAATAAGGATGAT1480.10220006352976922No Hit
TAACAGGGGGATTCTTGCCGCTGGCCCAGTCACTATGTAGTGGAGGGGCAGA1480.10220006352976922No Hit
ATGCTAGGGCTGATAACAAAAAGTGGAAGTTGCAGTTACATGAACTGACTAA1480.10220006352976922No Hit
TGCGGGGGAGCCTCCGATGCTCTGTGAAATGGAAGGAATGTGCTTGCCTGTC1480.10220006352976922No Hit
TCTACGGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1470.10150952255997348No Hit
GCTACTGGGAGCTCATATCTACCTGTCTAGTCAATACATCAGCTTTGCTGGG1470.10150952255997348No Hit
GTTTGGGTAATACAGGAAGAACTCCTGCAACTCAATCAGAAAAACAACAGAT1470.10150952255997348No Hit
GATGTTGGGGCAGCGGCGGCCGGGGCTGGGAGCGGGCCCTCGGCGGCCCAGG1460.10081898159017774No Hit
ATTGTTGGGAGTACAGTCCTTGCATCCATTCTATTTTTAACTTCCATATCTG1460.10081898159017774No Hit
GTTATGGGGGGCTGTAGGGAGCCTGTGCTGTGCCGCGCAGTTAGGCAGCAGC1460.10081898159017774No Hit
TCCATGGGGCCTCTAGAAGACCTGAGTCTTCCTGACCGACTCCACCATCACC1450.100128440620382No Hit
TTGTTTGGGTGAATGAATATAGTCCCAAAATAATATGGTTAGTATTTTTGGT1450.100128440620382No Hit
TATTGGGGGTCACCAGCGCCAGCACAACAAGGACAAGCCCTACAAGTGTCCC1450.100128440620382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)