FastQCFastQC Report
Sat 31 Dec 2022
SRR5844398.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844398.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142556
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGC6160.43211088975560485No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGCCGTCT5960.41808131541289034Illumina PCR Primer Index 1 (95% over 23bp)
AATGTTGGGGATCTAGAGAGGGGTTGGAGAAAATAATGGAAGAGAGAGATAT3730.2616515614916244No Hit
CTATTAGGGATCTCATTGTGGCTTTAATTTGCATTTCTCTGATGATTAGTGA2610.18308594517242346No Hit
TTATGTGGGGAGGGGCTCGTGTGCTGGGCGACAGTGACACACTCAGCAGCCC2310.1620415836583518No Hit
TGTATGGGGTGGAGTCAGTTACGTTGTTAAGGATGCCTTTGGCTACAAGTAA2030.14240017957855158No Hit
AATTATGGGGCCTAGGCCCTGGTGAAACCACAGGTATGCAATTATGCTAGGG1990.1395942647100087No Hit
TTTTCAGGGGAAATTGATCAGCAGGCAAAATGCCCTTGAGGAGCTTATCTTC1950.13678834984146582No Hit
GGAGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1880.13187799882151574No Hit
CTAGACGGGAAGTAACACATTTGGCCACAAAACACCAAAGAATTGTAGGCAG1860.1304750413872443No Hit
GCGTGGGGGCTGCCAGCTGCGAGCCCCTGGCCTCCGTGCTCAGGGCCAAGCC1830.12837060523583715No Hit
CACACTGGGATATTAGTCTTTGGAAGTTTAGCTGCCTGGCCTCTTTCACTTA1820.1276691265187014No Hit
GTCTCGGGGAACCCGCCACATCTACCATCACCCTCTACATCACCGCCCCGAC1810.1269676478015657No Hit
CGGTCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1810.1269676478015657No Hit
ACGTTGGGCATCCGGGCCCGGGTGTCTTCCGGCAGCTCCTCTGAGGTCTCCT1810.1269676478015657No Hit
TCGGGGACCAGCCACTGCAAAAACAAACCAAAATATAAAGACCAATGAGACT1800.12626616908442997No Hit
GGGAACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAAC1780.12486321165015853No Hit
CAGTGCGGGAGGAATATACACACATAAGTCAAAAGAAGACATGTCTACAGAG1740.12205729678161564No Hit
TCGCCAGGGATGGGCCAAACAGGTCCCATCCTCTTCATGACAGCTGTGAGCT1730.12135581806447993No Hit
GCACCAGGAGAGGTTGCAGTGAGCCGAAAGTGCGCCACTGCGCTCCAGCCTG1720.1206543393473442No Hit
CCAGGCGGGCATCCCGGGTGCGATTTGGTCAACAAAAGCGATACCAAGATTG1680.1178484244788013No Hit
GCGTATGGGATAGATATTTCTCAAAAAAAGACAAACAAATGGCAAACAGGCA1680.1178484244788013No Hit
TGTGCCGGGGTGTTACTAAAGTGTCTAGTTTTTTGAAATGCAAAGCTTTATG1640.11504250961025843No Hit
TCACTGGGCTAGCTCTGGGACTGGTGATGAGATCGGGAAAGTGGGCTCAGGA1640.11504250961025843No Hit
TGCTATGGGATATAATGGTTTGCAGGAACAAAAGTATAATGTGACTTGCAAG1620.11363955217598698No Hit
GCAAACGGGGAAATCATAACAAACAATCTCTCAGACCACAGTGCAATCAAAT1610.11293807345885126No Hit
GCTTATGGGATCCCCAGTGTGCCCCCGGCCCCGGGTCCGCCTTGGCTCGGAG1590.1115351160245798No Hit
TGCTAAGGGAGATATGGTGACATTCAGCTGGCATGTGGAAATGAATTATTGT1590.1115351160245798No Hit
ACGATAGGGCTGAACTCAGGGCACGACTAGAAGCCCTCAAAACCCCTGGGAC1590.1115351160245798No Hit
GATGTTGGGGCAGCGGCGGCCGGGGCTGGGAGCGGGCCCTCGGCGGCCCAGG1560.10943067987317266No Hit
AATTGTGGGGGCGCCGGAAGTGGAAGACCAGGCAGCCCAGCTGAAGGCAGTA1560.10943067987317266No Hit
TATACTGGGTAAGACACAGAGCTGCTTATCATCCAGACTTTCCAACAGTTCT1560.10943067987317266No Hit
AGAAGAGGGAATTTGGGGCTAGTAAAGAGAAGAAAATAAAAATAAGCAAAAC1550.10872920115603693No Hit
GTGGCAGGGCAAGGGTTAAAGGGGTGACATTGAGGGATGCCTCAGAGTCAAA1540.10802772243890121No Hit
TATCTGGGGGGCGGCCGGCCAGTTCCAGCCCGCACCCCGCGTCGGTGCCCGC1540.10802772243890121No Hit
AATTGGGGGGGAATTAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGG1540.10802772243890121No Hit
TATTGCGGGGAAGAGATTAACAGGTTTACCAAAGATTATTTTTATAACCTCT1530.10732624372176548No Hit
CAGCCTGGGCAACAAGACCGAAACTCCGTCTCAGATAAAAAACAAACAAACA1530.10732624372176548No Hit
GCGAGGGGAAATCAGCAGAGGTGGAATTGAAGACAGGAGGAAATAATTCAAA1530.10732624372176548No Hit
TCTTTAGGGCAAAGCTGCAGAGTGAAGATAATATTGGACTAATAATTGGATT1520.10662476500462977No Hit
GAACTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1520.10662476500462977No Hit
TGACCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1510.10592328628749403No Hit
CTTTCATGGGGTCTCTAGTCTGGGTAGATTAATCTGGGGTACAAGTATATTT1510.10592328628749403No Hit
ATGCGAGGGGTCTTCAAACTGAAAATTTAGAATGAACATTTCAAAGTAGGAA1510.10592328628749403No Hit
TCGACGGGGGTGTTGATGTGTGTCCTGCAACCTGGGGCATCAACATCCTAAT1510.10592328628749403No Hit
TTCCCAGGGAATATAAATACTCTCAAAATGGTTGTATGGATTTTATTTAGGT1500.10522180757035832No Hit
TCGTAAGGGGCTCTCAGATTGTTTAGCTTGTTTGACAGCTCTGTAGACTTAT1500.10522180757035832No Hit
TGTGCTGGGATCCTCAATCTTGCTGCAGTGTCGGGCAGTCCTCCTATTGGCA1490.10452032885322259No Hit
CAGCTTGGGCAGATTATGCTTACCTCAGGTTTCTTTAGTGTGCTTGAATGCC1460.10241589270181543No Hit
TTTCCAGGGACACAAATGAAATGGAAACACAACATACCAAAACCTATGGCAT1450.10171441398467972No Hit
TGCGTTGGGCTGTCTCTCTAGCCCTATGAGATTGCTAAAATTACTGATGCCT1450.10171441398467972No Hit
CAGCTGGGTAAAGCGGGAGGTAGACATGTAGCAGTAAAAATAGTTAAAAATG1440.10101293526754399No Hit
AGCTTAGGGGGAGAACTACAAACTACTGCTCAAATAAATCAGAGATGACATA1430.10031145655040827No Hit
AACAATGGGAGAGGCCCCGGGGAGCTGTCTTGCCCTATTCTACTGTGGGTGG1430.10031145655040827No Hit
TTAAAGGGAAGAGAAAGGAGGAAAGATCTAAAATCGACACCCCAACATCACA1430.10031145655040827No Hit
GGATCAGGGATAGAGAACAATTTTGAAGTTCCTCAAAAAACTAAAAACTGAG1430.10031145655040827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)