FastQCFastQC Report
Sat 31 Dec 2022
SRR5844394.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844394.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169577
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGCCGTCT12900.7607163707342387Illumina PCR Primer Index 1 (95% over 23bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGC11950.7046946225018723No Hit
ATCCTAGGGATGGCCAAACTTCTTCCAGGAAGAATGGTAGGCCTGGGATGAG2570.15155357153387547No Hit
TACTATGGGAGAGTTCCAGAGAGAAAAAGAGGAAAGAAAGATGGGAAAAAGG2410.14211832972631902No Hit
AACAATGGGAACTGATGCAAGAAAGATTCACTGATTACCAAAATTAGTGGGG2400.14152862711334674No Hit
GGCATAGGGGACTGCGCGCAGGCATCGTCCCGGCCGCCGTCACCGCCGCGCC2260.13327279053173485No Hit
ATTAGCGGGGGAGACCTTTGGGTTGATGGCAAGATTTGGAGGGCTGATTATG2160.12737576440201206No Hit
GTGGATGGGGGAAATCCAGCAGCAGATCTGGATCCCGTCTGTATTTATTGGG2130.12560665656309525No Hit
AGGCACGGGGGCTTTCAGCTCCAGTATCAAATGATTTTTTCAATATGGCTCC2120.12501695395012294No Hit
TTTGCAGGGACTATTTCAAGGCAGACCACTCTAACAGAAGTATAGATATGTC2120.12501695395012294No Hit
CATTTTGGGAGTTGGTTTTTTGAAAAGATAAATAAAAATGGACAAATCTTCA2120.12501695395012294No Hit
AGCGCTGGGCTGTGATATTTGCCAGTTTGGTGCAGAATGTGACGAAGATGCC2100.12383754872417839No Hit
GCAGTAGGGAATGATGATGATGGACTAAATCAGCAGATTCCTAGGAAGGAAA2000.11794052259445562No Hit
CTGACACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1990.11735081998148333No Hit
TATGCAGGGAGAGCAGCTGCTGGAGGGGAATCGAGGCGCGGCTCCGGGGATT1950.11499200952959422No Hit
AATCCAGGGAGATTCCTAGAGGCCCGATTCCGCTAGCCCGGAACAGACAAAG1920.11322290169067739No Hit
GACACAGGGCTGTCAAATTTTGTCATGGTAAATACTGGGGCTATACTGGCTA1920.11322290169067739No Hit
TTTGCAGGGATCCTGACACCATTCTCTCTCCATTTACTTCTGGTGGTTACCC1900.11204349646473284No Hit
ATGCATGGGCTAGCTTGCAGCTCCCACTCAGATGGACAGAGTAGCATGTGGA1900.11204349646473284No Hit
CTAGTTGGGTGATAAAATGATATATGATTTCAAAAATCCTACTGAAGCTAAT1900.11204349646473284No Hit
ATAGATGGGTAAGTCAGACTTAAAGATTTGAGAAATTATTTGTGTCATTTAC1900.11204349646473284No Hit
GGGTTTGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1890.11145379385176055No Hit
GTCAGGGAATGTAACCACTTTCTTCTTTGAGTAGTTGATATAGCAAATAGTT1890.11145379385176055No Hit
TCCATTGGGGAGATGAAGAAATTTATTACGTAGCCAAAGATGCACACCGCAA1870.110274388625816No Hit
GCGGCCGGGATAAAGAGCTCCCATATACTCATTGCCAAATTCAGTAGTTGTA1870.110274388625816No Hit
AAAGCCGGGTGGTTGGAGTAAGAAAAATGCATTGATAAATATTACAAAACTG1840.10850528078689917No Hit
GAATGGGGGATATCAAAGTACAATGCTTAAGTTATACTCAGTAATAAAAGAG1830.10791557817392688No Hit
AGTAACGGGCTGGGGAGGCATTTGCCTCTGTACCATCGCCTTTGCCGCTGCC1810.10673617294798234No Hit
AAAGCAGGGAAGGATCACCGCTTTGAAAGTGTCAGTCACCTTATCAGCTACC1810.10673617294798234No Hit
TTAGTGGGGTACGAAGTGGACTTCCCGGTGTTTGTTTGTTTGCAATACACTC1810.10673617294798234No Hit
GGAGTCTGTCTGCCTGGATTTGAATTATGACTCTACCGCCTACTACCTAATC1790.10555676772203777No Hit
AGTTTTGGGACTGTTAAAATATTTCAAGTGGGACCATAAAGAAATAGATGCT1790.10555676772203777No Hit
CTTCTTGGGGGCATCAACAACCGACTAATCACCACCCAACAATGACTAATCA1790.10555676772203777No Hit
GCTGGCGGGGCCCTACGGTATTCTTCAAAGGCTAGTCCCTTGACAGCATAAT1790.10555676772203777No Hit
GGCCATGGGATGAGCCGATCTGGGGAGGTTGGTTTTATAGACAGAAAAGGGC1790.10555676772203777No Hit
CTTATTGGGGTGCTGGTGGATGGGGAGCTCAAAGTGACGGACCTGGATGACG1780.1049670651090655No Hit
GAATAGGGGACAGCCTCCCAAACACCGAGGAGAATACTTAAATAAGAGAACG1770.10437736249609322No Hit
TTTGCACGGGGGGCAGGTGATTTACCATCCAAGATGAAGAGTTTTAAAAGAA1770.10437736249609322No Hit
CATAGTGGGGTTGCACTATTTTTAATAGCAAAAATATGAAACAAATCTATTT1770.10437736249609322No Hit
AATTCTGGGGGGGGCGAGCGGAGGCGAGTGGAGGACGCGTAGACGCGCCGCG1770.10437736249609322No Hit
TGTTTTGGGGGGCTGAGCCAGCGGCTCTTGGGAGGCTGCGTCCGCACGCCGG1760.10378765988312096No Hit
ACAGATGGGCGGAGGGAGCCCTGGATTGGACTACGCAGAGTTGGGGACGAAT1760.10378765988312096No Hit
ACAGCTGGGAGAATATTGAAGAACTGTAGGAGAAATACAAAAGAAGTAACAT1760.10378765988312096No Hit
GTGTTTGGGCAGCACCTGAAGTGAGGCATTTCCAGCAAGCAACTCCACAGAC1740.10260825465717638No Hit
GATCAGGGGAAGTATACTGACTTGTCTTTTGGGAATGGAATTATGGATGACT1740.10260825465717638No Hit
CTGTGAGGGACTAGCCCTAGTTGATAGGTGAGGTGGGTGAAGAAGGCTGGAG1730.10201855204420411No Hit
CAAGCAGGGAGGCAGAAGCAAATGCTACAGCTATCAGTAATCTCTTGCCATT1730.10201855204420411No Hit
TTGCCCGGGGACACAGGTTTTTTCCATGGCTTTTACTTTGTCTTGTGTATGA1730.10201855204420411No Hit
TTGTAGGGGATGAGCAGCTCTTCCAGTGGGCAAAGAGGACGCCCATAATTTC1730.10201855204420411No Hit
GAATCCGGGTCAACTGGTGGAGATTGGCTGACACCCTGGAGAAGCCGAAACC1720.10142884943123182No Hit
TATTCCGGGTCTCCCCATTCTCTGCCCCCTCTCCACCTGTACCCTTATCCTG1720.10142884943123182No Hit
AGACTAGGGAATAGGAAACTTGAATTAAAATGAACAGATTTGTACATCCCCA1720.10142884943123182No Hit
AAATGCGGGCTGAGCGAGGGACGGCCAAATACTTTTGAAAACACCTTTCTAT1710.10083914681825955No Hit
AATGGAGGGGATAGAGATTAATACAACTACTTAAAAAATATAGTCAATAGGT1710.10083914681825955No Hit
ATTTATGGGCCACGAGGAGCTGACACGTCCTCTCTGGCCGCCATCCGGCTCG1710.10083914681825955No Hit
ACACTGGGGGATGAAAGCTGACACGCAGCTCTCAGAATCACCCTTGCTATCA1710.10083914681825955No Hit
GGTCCAGGGAAGTCATCACTTCTTATTTTACCTATAAATGTTGATTAAACAG1710.10083914681825955No Hit
GTTGCTGGGGTTTATAGCTCAATAGTAAGAAGACACAACCCAGTTGAAAAAT1710.10083914681825955No Hit
ATTCTAGGGGTTTTTTCTATCTCCTTAGTGCCTCTTCTAATGATATGAAGTT1700.10024944420528727No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)