FastQCFastQC Report
Sat 31 Dec 2022
SRR5844393.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844393.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170293
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGC12120.711714515570223No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGCCGTCT12090.7099528459772275Illumina PCR Primer Index 1 (95% over 23bp)
ATCCTAGGGATGGCCAAACTTCTTCCAGGAAGAATGGTAGGCCTGGGATGAG2620.15385247778828257No Hit
TACTATGGGAGAGTTCCAGAGAGAAAAAGAGGAAAGAAAGATGGGAAAAAGG2620.15385247778828257No Hit
AACAATGGGAACTGATGCAAGAAAGATTCACTGATTACCAAAATTAGTGGGG2390.14034634424198292No Hit
TTTGCAGGGACTATTTCAAGGCAGACCACTCTAACAGAAGTATAGATATGTC2230.13095077307933972No Hit
GGCCATGGGATGAGCCGATCTGGGGAGGTTGGTTTTATAGACAGAAAAGGGC2220.13036354988167453No Hit
AATTCTGGGGGGGGCGAGCGGAGGCGAGTGGAGGACGCGTAGACGCGCCGCG2200.12918910348634413No Hit
TGCGCAGGGGAATCACTTGAGCCCGGGAGGTGGAGGTTGCAGTGGGCCGGGA2150.12625298749801814No Hit
ATTAGCGGGGGAGACCTTTGGGTTGATGGCAAGATTTGGAGGGCTGATTATG2130.1250785411026877No Hit
TCCATTGGGGAGATGAAGAAATTTATTACGTAGCCAAAGATGCACACCGCAA2090.12272964831202691No Hit
TGAACAGGGCCGATCTAACTGAGGGATTTTCCTTTTCCTAGGTAAAGTATGG2080.12214242511436173No Hit
AGCGCTGGGCTGTGATATTTGCCAGTTTGGTGCAGAATGTGACGAAGATGCC2020.1186190859283705No Hit
CTGACACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG2010.11803186273070532No Hit
GACACAGGGCTGTCAAATTTTGTCATGGTAAATACTGGGGCTATACTGGCTA2000.11744463953304013No Hit
AAATTAGGGATTCACTGAATCTTCTGTGCTTAAACGACATGAGAGAATTCAC1980.11627019313770971No Hit
GCAACAGGGAATCAAAGTTGACGTCAAACATCTTACTACAAAAATTCAGTGA1980.11627019313770971No Hit
ATGCAAGGGTAGAAAGTCAGATGACTACCGTGTTGGTTGTGACTTCCCCTTA1970.11568296994004451No Hit
TATCTTGGGTCATTATTATGAACCTTTTGGACCTGATGAATGTACAATGTAC1960.1150957467423793No Hit
GTTGCTGGGGTTTATAGCTCAATAGTAAGAAGACACAACCCAGTTGAAAAAT1950.11450852354471411No Hit
ACAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATA1930.11333407714938372No Hit
CGCTGAGGGAGGGTAGATTTCGAAGCACTGGTTCTGTTGAAGTTAAGTTTAT1930.11333407714938372No Hit
GTGGATGGGGGAAATCCAGCAGCAGATCTGGATCCCGTCTGTATTTATTGGG1930.11333407714938372No Hit
CTTATTGGGGTGCTGGTGGATGGGGAGCTCAAAGTGACGGACCTGGATGACG1900.11157240755638811No Hit
GGCATAGGGGACTGCGCGCAGGCATCGTCCCGGCCGCCGTCACCGCCGCGCC1900.11157240755638811No Hit
ACACTTGGGGGAGAATGAAGATAAGTAATTTACCTGTCCAGGATCAAAAGAA1890.11098518435872291No Hit
AAAGCCGGGTGGTTGGAGTAAGAAAAATGCATTGATAAATATTACAAAACTG1880.1103979611610577No Hit
CATTTTGGGAGTTGGTTTTTTGAAAAGATAAATAAAAATGGACAAATCTTCA1880.1103979611610577No Hit
TAGCATGGGGAAACTAAGGCTTGTTGGTGACAGCTTGGTAATAGTATTCTAC1880.1103979611610577No Hit
GCTAGTGGGATCTTCATTAAATATTTCAATATCACCTATCCCTGGCTTATAT1870.10981073796339251No Hit
TTTGCAGGGATCCTGACACCATTCTCTCTCCATTTACTTCTGGTGGTTACCC1870.10981073796339251No Hit
ATGAGAGGGATCCCTGTGTGTGCCGTGCGAGGACGCAACGTCGAGAACATGA1870.10981073796339251No Hit
CATAGTGGGGTTGCACTATTTTTAATAGCAAAAATATGAAACAAATCTATTT1870.10981073796339251No Hit
GCGAACGGGGAGACTCCGGAGTCGCCAACATGTCGACCGCCATGAATTTCGG1850.1086362915680621No Hit
ATGTTAGGGTAAGGAAAGAAGCAATCAGTAGAGAATTCAGGATAGTTTTGTT1840.1080490683703969No Hit
GAATCCGGGTCAACTGGTGGAGATTGGCTGACACCCTGGAGAAGCCGAAACC1830.1074618451727317No Hit
ATTTATGGGCCACGAGGAGCTGACACGTCCTCTCTGGCCGCCATCCGGCTCG1830.1074618451727317No Hit
TGATGCGGGCAATGAAGAAAAAAACAACGTATTCAGGCAACAAATAGCACGA1830.1074618451727317No Hit
ACAGATGGGCGGAGGGAGCCCTGGATTGGACTACGCAGAGTTGGGGACGAAT1830.1074618451727317No Hit
CTTGATGGGGTTTTATGACTATTTATTGCAAGGTCAGATTTACAGATTGTTA1830.1074618451727317No Hit
CAAAATGGGAGAGCATAATTTTGCAGCCACAAACGTTTTTCAACCAAAAATT1810.1062873987774013No Hit
CTAAGGGGGGCTAAGGAGGCCAGTCCAGGAGATGGTCCCTGAGGTAGGATGG1810.1062873987774013No Hit
GAATGGGGGATATCAAAGTACAATGCTTAAGTTATACTCAGTAATAAAAGAG1800.1057001755797361No Hit
GTGAAGGGGATGAAAAAACAAGACCCAGTGGCCTGTCGCCTATAGGAAACAC1800.1057001755797361No Hit
GGAGTCTGTCTGCCTGGATTTGAATTATGACTCTACCGCCTACTACCTAATC1790.10511295238207091No Hit
AGTAACGGGCTGGGGAGGCATTTGCCTCTGTACCATCGCCTTTGCCGCTGCC1790.10511295238207091No Hit
TCAGTTGGGACCGCCTGGATCCTCAGCTCCAGCTGCACTGCTCAGACGAGAT1790.10511295238207091No Hit
GTGTTTGGGCAGCACCTGAAGTGAGGCATTTCCAGCAAGCAACTCCACAGAC1790.10511295238207091No Hit
GTTAAGGGGATGTGTTCCATGGCTGGGAATTGCCTACCACCCACATCAGCTT1790.10511295238207091No Hit
TAAGCGGGGGAGTTAGCACAGACTGCAAAAGTTATGGGCTCAGTTTTCTCCA1790.10511295238207091No Hit
AGCTTTGGGGGCATTACAAGGAGCCAGTCAAATTATTGCTGAAATCCGGGAG1790.10511295238207091No Hit
CATAATGGGAGCTCAGGGCCGAGCTGTGCCGCCAGCGGACTGAGTACGAGAG1790.10511295238207091No Hit
GTCAAGGGGGGCCCCTGTCTCATATCCAAATAGTAATAGAGGCTCAAGCATT1780.1045257291844057No Hit
CTATGTGGGGAAAAAAGTAAAACTTGCTGCTGATGAAGATGATGACGATGAT1780.1045257291844057No Hit
AGGACAGGGGGATAGACTAAGGAGAAGATCAGCAAAGAAAAAAATGATGTGA1780.1045257291844057No Hit
ATTCTAGGGGTTTTTTCTATCTCCTTAGTGCCTCTTCTAATGATATGAAGTT1780.1045257291844057No Hit
CGCTGGGGCGGCGCCCTTATTCCCCTGCCTTCCTGGCTTGATTGAAGGAAAG1770.1039385059867405No Hit
GAATTTGGGGCATTCATCTATTCTTGAGGGATCCATCTCCCTCACCCAAACA1770.1039385059867405No Hit
AATGTAGGGAGGATAACCAGTGTAGAGAAGAACATAAATGACCTGATGGAAC1770.1039385059867405No Hit
ACAGCTGGGAGAATATTGAAGAACTGTAGGAGAAATACAAAAGAAGTAACAT1770.1039385059867405No Hit
CTGTGAGGGACTAGCCCTAGTTGATAGGTGAGGTGGGTGAAGAAGGCTGGAG1760.10335128278907531No Hit
AGGCACGGGGGCTTTCAGCTCCAGTATCAAATGATTTTTTCAATATGGCTCC1760.10335128278907531No Hit
TCTTGCGGGACACACATTTGCAATATAATTTGATGAGTAATGTTATAGGGGA1760.10335128278907531No Hit
AGACTAGGGAATAGGAAACTTGAATTAAAATGAACAGATTTGTACATCCCCA1760.10335128278907531No Hit
CTGTCCGGGTCTCTCGTTGGGGTGACTCACAGTCACAAAGTAAGCACATGCT1750.1027640595914101No Hit
TATTCCGGGTCTCCCCATTCTCTGCCCCCTCTCCACCTGTACCCTTATCCTG1740.10217683639374489No Hit
ATGGCCGGGCAGCAGTTCCAGTACGATGACAGTGGGAACACCTTCTTCTACT1740.10217683639374489No Hit
TATGCAGGGAGAGCAGCTGCTGGAGGGGAATCGAGGCGCGGCTCCGGGGATT1740.10217683639374489No Hit
GTCAGGGAATGTAACCACTTTCTTCTTTGAGTAGTTGATATAGCAAATAGTT1740.10217683639374489No Hit
AACCAAGGGGTGGCAGCAACCTCAGGAGATGGCTACCGGGGTCAGACCTCCC1740.10217683639374489No Hit
CGCCATGGGGTATGAACAGTTATTTAAATAAACAAAATTCTTTGGGTCTGAC1730.1015896131960797No Hit
AAAGCAGGGAAGGATCACCGCTTTGAAAGTGTCAGTCACCTTATCAGCTACC1720.1010023899984145No Hit
ATTAGCGGGCTCCCCAACCTCAAAAGAAAACAAAAAATTATTTTTTTAAATT1720.1010023899984145No Hit
ACGTCCGGGGATCTCAAATCTGCTGTTTTCATTTACTTTTTTTGGTTCATGA1710.1004151668007493No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)