FastQCFastQC Report
Sat 31 Dec 2022
SRR5844392.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844392.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168189
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGC11880.7063482154005316No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGCCGTCT11200.6659175094685146Illumina PCR Primer Index 1 (95% over 23bp)
ATCCTAGGGATGGCCAAACTTCTTCCAGGAAGAATGGTAGGCCTGGGATGAG2460.14626402440112019No Hit
AACAATGGGAACTGATGCAAGAAAGATTCACTGATTACCAAAATTAGTGGGG2160.1284269482546421No Hit
GGCCATGGGATGAGCCGATCTGGGGAGGTTGGTTTTATAGACAGAAAAGGGC2130.12664324063999427No Hit
TACTATGGGAGAGTTCCAGAGAGAAAAAGAGGAAAGAAAGATGGGAAAAAGG2090.12426496382046388No Hit
TCAAAAGGGTCTTTCTTGAATGCTTTAGTTTCTTTCAGGGAGTTGATAATAT2070.12307582541069867No Hit
CTAAGGGGGGCTAAGGAGGCCAGTCCAGGAGATGGTCCCTGAGGTAGGATGG2060.12248125620581607No Hit
CATTTTGGGAGTTGGTTTTTTGAAAAGATAAATAAAAATGGACAAATCTTCA2060.12248125620581607No Hit
AATTCTGGGGGGGGCGAGCGGAGGCGAGTGGAGGACGCGTAGACGCGCCGCG2040.12129211779605088No Hit
CTGACACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG2040.12129211779605088No Hit
AAAGCCGGGTGGTTGGAGTAAGAAAAATGCATTGATAAATATTACAAAACTG2030.12069754859116827No Hit
TTTGCAGGGACTATTTCAAGGCAGACCACTCTAACAGAAGTATAGATATGTC2000.11891384097652045No Hit
CAAAATGGGAGAGCATAATTTTGCAGCCACAAACGTTTTTCAACCAAAAATT1970.11713013336187267No Hit
GTGGATGGGGGAAATCCAGCAGCAGATCTGGATCCCGTCTGTATTTATTGGG1970.11713013336187267No Hit
ATTAGCGGGGGAGACCTTTGGGTTGATGGCAAGATTTGGAGGGCTGATTATG1930.11475185654234224No Hit
GTTGCTGGGGTTTATAGCTCAATAGTAAGAAGACACAACCCAGTTGAAAAAT1930.11475185654234224No Hit
GCGGCCGGGATAAAGAGCTCCCATATACTCATTGCCAAATTCAGTAGTTGTA1930.11475185654234224No Hit
GAATCCGGGTCAACTGGTGGAGATTGGCTGACACCCTGGAGAAGCCGAAACC1910.11356271813257703No Hit
AAATTAGGGATTCACTGAATCTTCTGTGCTTAAACGACATGAGAGAATTCAC1910.11356271813257703No Hit
TTTGCTGGGAGGCCGAGGGTGGGGCTGCCACGGCAGGGCCCAGCCGGGAACA1900.11296814892769445No Hit
TATTCCGGGTCTCCCCATTCTCTGCCCCCTCTCCACCTGTACCCTTATCCTG1890.11237357972281184No Hit
GGGTTTGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1890.11237357972281184No Hit
AGTTACGGGAGGTCAAATTTACCTAAAGTGAATACAGCCCATTCTCAATTAT1890.11237357972281184No Hit
GCAGTAGGGAATGATGATGATGGACTAAATCAGCAGATTCCTAGGAAGGAAA1890.11237357972281184No Hit
AAAGCAGGGAAGGATCACCGCTTTGAAAGTGTCAGTCACCTTATCAGCTACC1860.11058987210816403No Hit
AGCGCTGGGCTGTGATATTTGCCAGTTTGGTGCAGAATGTGACGAAGATGCC1850.10999530290328143No Hit
CTGTGAGGGACTAGCCCTAGTTGATAGGTGAGGTGGGTGAAGAAGGCTGGAG1840.10940073369839881No Hit
TGATGCGGGCAATGAAGAAAAAAACAACGTATTCAGGCAACAAATAGCACGA1840.10940073369839881No Hit
CTTGATGGGGTTTTATGACTATTTATTGCAAGGTCAGATTTACAGATTGTTA1820.10821159528863362No Hit
TATGCAGGGAGAGCAGCTGCTGGAGGGGAATCGAGGCGCGGCTCCGGGGATT1810.10761702608375102No Hit
TCGTACGGGTAATGATGAAGTGGTAAATCTCAATTCTCTCTGGCCTGGTGCT1790.10642788767398581No Hit
ATGCATGGGCTAGCTTGCAGCTCCCACTCAGATGGACAGAGTAGCATGTGGA1790.10642788767398581No Hit
AGTAACGGGCTGGGGAGGCATTTGCCTCTGTACCATCGCCTTTGCCGCTGCC1780.10583331846910321No Hit
TCCATTGGGGAGATGAAGAAATTTATTACGTAGCCAAAGATGCACACCGCAA1770.1052387492642206No Hit
GCTAGTGGGATCTTCATTAAATATTTCAATATCACCTATCCCTGGCTTATAT1760.10464418005933801No Hit
ACGTAGGGGGGGTGACGCCAGAGAGTGCGCGTCAGCAGTTTATTAGAGAGCT1760.10464418005933801No Hit
TTATCAGGGGCTGTGTGTCCATCAACACACAAATTCGTAAAAAACACACATG1760.10464418005933801No Hit
GACACAGGGCTGTCAAATTTTGTCATGGTAAATACTGGGGCTATACTGGCTA1730.1028604724446902No Hit
GTCAGGGAATGTAACCACTTTCTTCTTTGAGTAGTTGATATAGCAAATAGTT1720.1022659032398076No Hit
TTAGTGGGGTACGAAGTGGACTTCCCGGTGTTTGTTTGTTTGCAATACACTC1720.1022659032398076No Hit
CGACGAGGGTAGACTTCTTGGAGGTTTTTCAGGAGATATTCTGTCAGCTCTT1720.1022659032398076No Hit
TTTGCAGGGATCCTGACACCATTCTCTCTCCATTTACTTCTGGTGGTTACCC1710.101671334034925No Hit
TGTTTTGGGGGGCTGAGCCAGCGGCTCTTGGGAGGCTGCGTCCGCACGCCGG1710.101671334034925No Hit
ATAGATGGGTAAGTCAGACTTAAAGATTTGAGAAATTATTTGTGTCATTTAC1710.101671334034925No Hit
TTGACAGGGTGTTGCACTGATCAGATACATTCCTTTTAGGGTACTAAATTTG1710.101671334034925No Hit
ATCCGAGGGCTATGGAAAAGAAAAGAAAGTAAGCAAACAACAGATTAATTTA1710.101671334034925No Hit
GGCATAGGGGACTGCGCGCAGGCATCGTCCCGGCCGCCGTCACCGCCGCGCC1710.101671334034925No Hit
CTTATTGGGGTGCTGGTGGATGGGGAGCTCAAAGTGACGGACCTGGATGACG1700.10107676483004238No Hit
AGGACAGGGGGATAGACTAAGGAGAAGATCAGCAAAGAAAAAAATGATGTGA1700.10107676483004238No Hit
TGCGCAGGGGAATCACTTGAGCCCGGGAGGTGGAGGTTGCAGTGGGCCGGGA1690.10048219562515978No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)