FastQCFastQC Report
Sat 31 Dec 2022
SRR5844352.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844352.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283384
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGCCGTCT10790.3807554413798944Illumina PCR Primer Index 1 (95% over 23bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGC10550.3722863676142619No Hit
CTTAACGGGATATTTTGGTAAGTTTCATCTATTTTACCTAAAGTAATTGAAT4100.14468001016288853No Hit
TCGCGAGGGTAGAGAAACATGGCATCTCTCAAACTGGCAATATTTCCCAGGT3430.12103717923383113No Hit
GACCACGGGTAGGCTTAGGTTTATGTGTGTGTATGTGTCTTAGTTTTTAACA3310.11680264235101488No Hit
GAGGAAGGGCAGCTGTCTGAAGGCAGAGACCATGATTTGTATCCATAGTATT3300.11644976427744687No Hit
ACACCAGGGATTACAGGGTAGACACGTTTTAACTGAAATCAATCAAGATAAC3230.1139796177624707No Hit
ACGTGCGGAGGCATTTTGAGGTGTCTCTTTGGGTTCTTTCTGTTTGAAAGGA3200.11292098354176663No Hit
GCTAAAGGGGCTCTCTTCAAACCCAATTTTCAGGCCCTACTCTTTTCCAAGT3190.11256810546819862No Hit
GAATCAGGGGCCTCAGTGTCTTTGCCAGAAAAAGGCAAAGAGGCGGACAGTG3160.11150947124749457No Hit
TCTAATGGGGGCCGTCGGCTCCGCGGCGCCGCCATGGCCGACGTGGAAGACG3140.11080371510035851No Hit
TAAATAGGGCGATCTATGAACGGGGCTTTCCGTCCCGGGCAGCCAGCGCCAG3060.10798069051181436No Hit
TTCGACGGGGTTCAGAGATAAGAAATCCAAATCACAGTGTAGTTTGTTTCAA3050.10762781243824633No Hit
CTGTAGAAGGGAGTGAAGCATCAGTATACTGAAATCAGCCAGCAAGCTTTCT3020.10656917821754226No Hit
TAACTGGGTATTACCTCCAGTTAAGAGCCATTGAGCTAGAGAGTGCCAAGAA3010.10621630014397425No Hit
TAGTCCGGGCAGTTTATCAGAGTTGAAAGCAGAGAAAAAACTTATTTTTAAA3000.10586342207040624No Hit
TAAGATCAACTGTTTAGTGCCTTAATTGCATCTGCAAAATCCCTTCAAAATA2990.10551054399683822No Hit
CACAGGGGATTTTGCAAGTGGATATTCAATTTTCTCAACACCACTTGTTGAA2980.1051576659232702No Hit
TGACACGGGGATCTCAATTTGGGGGCATATGATTCCATGAATAAATGAATAA2970.10480478784970218No Hit
TCACTGGGGGGATGACGACGATGGTGGTTTTGTCAAAAAGAAAAGGGGGAAA2970.10480478784970218No Hit
AACCAAGGGAAGTTTTAAAAATTGTTATACATTAGACCTGAAAAAAATTAGA2960.10445190977613417No Hit
TTTAACGGGATTCATGAACAGAGTTGAAGTTAAAGTAAAGATTCCTGAAGAG2960.10445190977613417No Hit
TATTAAGGGTGTGGTGGCCGTGTGCTACGGCTCCTTCTACAATCTCCTCACC2950.10409903170256612No Hit
AAGGCAGGGAAGGACTTCTGGTCCAGACAAAATAGCATAGATCCATTTCTGT2940.10374615362899811No Hit
ATGGCCGGGATGTTTCCAGGGGTCAGTCTGTCAGGCAGGAAGGACCCAGGAT2880.10162888518758999No Hit
GATGCCGGGATTGAGAATTCAGCTCCTTAAAATTGGCAGTGCTATCCAGCGA2860.10092312904045393No Hit
GTATATGGGGCGCGAGTTGGACTGTGAAGAAACATGGCGGCCGCGACGTTGA2860.10092312904045393No Hit
TGTGCAGGGTTGACAAACACGTCCTACTTTTAGATACAAGCACGACTGAGGC2850.10057025096688592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)