FastQCFastQC Report
Sat 31 Dec 2022
SRR5844351.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844351.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284722
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGC10760.37791249007804106No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGCCGTCT10740.3772100505054053Illumina PCR Primer Index 1 (95% over 23bp)
TCGCGAGGGTAGAGAAACATGGCATCTCTCAAACTGGCAATATTTCCCAGGT3700.12995132093761635No Hit
CTTAACGGGATATTTTGGTAAGTTTCATCTATTTTACCTAAAGTAATTGAAT3600.1264391230744375No Hit
ACGTGCGGAGGCATTTTGAGGTGTCTCTTTGGGTTCTTTCTGTTTGAAAGGA3540.12433180435653024No Hit
TATGCCTGGGATAATAAACAGAATTATTTGTAGGAATGGCAACTCATACTAT3410.11976594713439775No Hit
GACCACGGGTAGGCTTAGGTTTATGTGTGTGTATGTGTCTTAGTTTTTAACA3340.11730740863017258No Hit
GAGGAAGGGCAGCTGTCTGAAGGCAGAGACCATGATTTGTATCCATAGTATT3330.11695618884385471No Hit
ACACCAGGGATTACAGGGTAGACACGTTTTAACTGAAATCAATCAAGATAAC3310.11625374927121894No Hit
TGTTCTGGGAACCTCACCTTGATCAGGTAAAGAAAAAAAATCTAAATCTAAA3250.11414643055331164No Hit
TTCGACGGGGTTCAGAGATAAGAAATCCAAATCACAGTGTAGTTTGTTTCAA3240.11379521076699377No Hit
GCTAAAGGGGCTCTCTTCAAACCCAATTTTCAGGCCCTACTCTTTTCCAAGT3190.11203911183540437No Hit
TAAATAGGGCGATCTATGAACGGGGCTTTCCGTCCCGGGCAGCCAGCGCCAG3160.11098545247645072No Hit
CTGTAGAAGGGAGTGAAGCATCAGTATACTGAAATCAGCCAGCAAGCTTTCT3150.11063423269013282No Hit
GAATCAGGGGCCTCAGTGTCTTTGCCAGAAAAAGGCAAAGAGGCGGACAGTG3140.11028301290381495No Hit
TCACTGGGGGGATGACGACGATGGTGGTTTTGTCAAAAAGAAAAGGGGGAAA3090.10852691397222555No Hit
CACAGGGGATTTTGCAAGTGGATATTCAATTTTCTCAACACCACTTGTTGAA3070.10782447439958978No Hit
TAACTGGGTATTACCTCCAGTTAAGAGCCATTGAGCTAGAGAGTGCCAAGAA3070.10782447439958978No Hit
TGTGCAGGGTTGACAAACACGTCCTACTTTTAGATACAAGCACGACTGAGGC3050.10712203482695401No Hit
TAGTACGGATTGCAACACCTTCGTGTACAGCAGCAGCAAAGGGACAATCCGG3030.10641959525431825No Hit
TCTAATGGGGGCCGTCGGCTCCGCGGCGCCGCCATGGCCGACGTGGAAGACG3030.10641959525431825No Hit
TAGTACGGGAGTTCAGTGTGTGTCTGAGATTCCTTGGAGAAACAACAACAGC3020.10606837546800035No Hit
TATTAAGGGTGTGGTGGCCGTGTGCTACGGCTCCTTCTACAATCTCCTCACC3000.10536593589536461No Hit
CTATGTGGGAAACATGGTTCACTCTCATGTTATTTTTCCTTTGATCAGCTTT2970.10431227653641097No Hit
TGCCAAGGGTAGAATGAAATGTTTATGGTTTATGGAATAGACGTTTATGGTT2960.10396105675009307No Hit
TAGCTCGGGCCTGCTTGAGGTGAATTCATTATCACAAGACAGTCCTAAATCA2920.10255617760482154No Hit
CATTTTGGGTGTTCAAGAACTCCTTCTGGACTGTGGATACTGAATCAGTGTA2920.10255617760482154No Hit
CCTCTTCCTCCCAGTGTTGCTGCAGCTAGCCAGCGGCTGAGTTATCGGAGAA2910.10220495781850367No Hit
CCAATAGGGCAAGGGCCTGTTGAATAAGTGAATGAGCTAAAATATACTGGTT2900.1018537380321858No Hit
GTTATAGGGAAATGGACGGCTTCCGGACCTCATCTCCGATGCTCTTTTCTGC2900.1018537380321858No Hit
CCGAACGGGGTTTTGTTTTTGTATGTTTTTTACCCCCATGTAACAGTCTCCC2880.10115129845955002No Hit
CATGGCGGGTGAGACGGAGTTTCGCTCTTGTTGCTCAGGCTGGAGTGCAATG2850.10009763910059637No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)