FastQCFastQC Report
Sat 31 Dec 2022
SRR5844350.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844350.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280921
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGCCGTCT10390.36985487023042063Illumina PCR Primer Index 1 (95% over 23bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGC9120.3246464308471065No Hit
CTTAACGGGATATTTTGGTAAGTTTCATCTATTTTACCTAAAGTAATTGAAT3540.12601407513144267No Hit
ACACCAGGGATTACAGGGTAGACACGTTTTAACTGAAATCAATCAAGATAAC3420.12174241156766494No Hit
ACGTGCGGAGGCATTTTGAGGTGTCTCTTTGGGTTCTTTCTGTTTGAAAGGA3280.11675880407659094No Hit
TCGCGAGGGTAGAGAAACATGGCATCTCTCAAACTGGCAATATTTCCCAGGT3280.11675880407659094No Hit
GACCACGGGTAGGCTTAGGTTTATGTGTGTGTATGTGTCTTAGTTTTTAACA3260.11604686014929463No Hit
TATGCCTGGGATAATAAACAGAATTATTTGTAGGAATGGCAACTCATACTAT3240.11533491622199835No Hit
TAACTGGGTATTACCTCCAGTTAAGAGCCATTGAGCTAGAGAGTGCCAAGAA3170.11284311247646137No Hit
GAGGAAGGGCAGCTGTCTGAAGGCAGAGACCATGATTTGTATCCATAGTATT3160.11248714051281322No Hit
TAGCTCGGGCCTGCTTGAGGTGAATTCATTATCACAAGACAGTCCTAAATCA3140.11177519658551692No Hit
CACAGGGGATTTTGCAAGTGGATATTCAATTTTCTCAACACCACTTGTTGAA3110.1107072806945725No Hit
AATGTCGGGGCTTCAAAGGAATAGGGTATTTCAAGGATAAATATATTAAGAA3110.1107072806945725No Hit
TGCCAAGGGTAGAATGAAATGTTTATGGTTTATGGAATAGACGTTTATGGTT3060.10892742087633177No Hit
TTTAACGGGATTCATGAACAGAGTTGAAGTTAAAGTAAAGATTCCTGAAGAG3030.10785950498538735No Hit
GAATCAGGGGCCTCAGTGTCTTTGCCAGAAAAAGGCAAAGAGGCGGACAGTG3020.1075035330217392No Hit
CTGTAGAAGGGAGTGAAGCATCAGTATACTGAAATCAGCCAGCAAGCTTTCT3000.10679158909444292No Hit
TCACTGGGGGGATGACGACGATGGTGGTTTTGTCAAAAAGAAAAGGGGGAAA3000.10679158909444292No Hit
TTCGACGGGGTTCAGAGATAAGAAATCCAAATCACAGTGTAGTTTGTTTCAA2970.1057236732034985No Hit
AGAGAGGGGATCTAAAAGCGGGTACATGATTCTGTGTTTTCTTGTGTGCTTA2960.10536770123985034No Hit
TGTTCTGGGAACCTCACCTTGATCAGGTAAAGAAAAAAAATCTAAATCTAAA2950.1050117292762022No Hit
GCTAAAGGGGCTCTCTTCAAACCCAATTTTCAGGCCCTACTCTTTTCCAAGT2920.10394381338525778No Hit
CCTCTTCCTCCCAGTGTTGCTGCAGCTAGCCAGCGGCTGAGTTATCGGAGAA2910.10358784142160964No Hit
CATTTTGGGTGTTCAAGAACTCCTTCTGGACTGTGGATACTGAATCAGTGTA2910.10358784142160964No Hit
TCTAATGGGGGCCGTCGGCTCCGCGGCGCCGCCATGGCCGACGTGGAAGACG2870.10216395356701705No Hit
TCGTCGGGGATGCCTATTGTAATATAGAAAAAAACATTCTATCTACTGATTT2850.10145200963972079No Hit
TGTGCAGGGTTGACAAACACGTCCTACTTTTAGATACAAGCACGACTGAGGC2820.10038409374877634No Hit
AACAGGGGTGTCCTCGGTATTTGAGGATCAGAACGCAGCAACCCATCTCATT2810.1000281217851282No Hit
TGTACAGGGTGTCTACATCTATAAGTGTATTTTTATCTTCATCTATCTAATC2810.1000281217851282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)