FastQCFastQC Report
Sat 31 Dec 2022
SRR5844343.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844343.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29461
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGC4581.5545976036115543No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGCCGTCT3561.2083771766063611Illumina PCR Primer Index 1 (95% over 23bp)
TGTCGCGGTGGAGGAGATGCCCATGAATGTGGCTGACCTTATTTAATTCCTG3011.021689691456502No Hit
TCTCTGGGAGTGTGTGAGAAAAGAATAAAAGTGAAATTTGCATAATGAATGT2800.9504090153083739No Hit
AGCACAGGGAACATCTATCATGCTGGAGGACTACTGTGATGGGGCTTTACAT2660.9028885645429551No Hit
GAACACGGGGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC2550.8655510675129832No Hit
GCATACGGGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGC2500.8485794779539051No Hit
GTTACAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC2490.8451851600420895No Hit
AAACATGGGGCTCCGACCCGGAACTGCTTTCTTGGGAAGATGGCGGCTGTGT2430.8248192525711958No Hit
GCATCTGGGTCGGGAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGAC2410.8180306167475646No Hit
TTAGAAGGGTGGTTCATTTGTAAATTATTATTCATATAGACCACTGTAGTAG2360.8010590271884865No Hit
CATATGGGGCTACTTGGGAATGCCCCAACTAAATACTACCGTATGGCCCACC2360.8010590271884865No Hit
TAACTAGGGCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTC2320.787481755541224No Hit
GCAGCCGGGCCTTATGAACACTGATGTAAAAATTTTCAACAGAATACTAGCA2290.7772988018057772No Hit
GTATATGGGGAGAGTGAGGATGAAAGCAATGAAGAGGAGGAAGAGGAAGATG2240.760327212246699No Hit
TGTTTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2230.7569328943348834No Hit
AGTATTGGGGGCGGCCCTCGCGGCAGGTTTCGGGCTTCAGGACAATTCGTGA2120.7195953973049116No Hit
GGCAAGGAGTTCCAGCGCCGCGCCAAGGCCATGATGCTGCGGGCAGCTGTGC2080.7060181256576491No Hit
ACGCCTGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC2060.6992294898340179No Hit
GGTAACTGGCACACTTCCCAAGCTCAAGAGCTCAAAAGAATGGTTGGAGCCC2060.6992294898340179No Hit
GTTATGGGGGTTCATTACCTCCTCCTACCCCAAGAGAAATGGATTCAGACAT2010.6822579002749397No Hit
TGTAATGGGACTAAGTAAGATCACAGATAGCATACAAATTCACTGACCATTA2000.6788635823631242No Hit
AAATTAGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1990.6754692644513085No Hit
GACGAAGGGTGAAAGATAGAGATTAATACAACTACTTAAAAAATATAGTCAA1980.6720749465394928No Hit
TGACGTGGGATAATAGAAAAAGTAATCATAAAATTTGAATGAAACCACAAAA1980.6720749465394928No Hit
CTTCCTGGGACTGGCGGGGAGCGCGTAACGAGAGACTGCTTGCTGCGGCAGA1970.6686806286276772No Hit
GCAGGCGGGCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTA1930.6551033569804148No Hit
CACCGTGGGGTGAGGCCAAATATCATTCTAAGGGGCCACAGTAATTACAAAC1930.6551033569804148No Hit
ATAAGTGGGATGGTGGTCATACTGCCAAGATATCTGATTTCTCCTGGAATCC1930.6551033569804148No Hit
TAGGGTGGGGGCCCAGCGCAGATCGCTTGCAGCTTGCTAGCTGTGTGGGCTG1920.6517090390685992No Hit
TCTGAAGGGTCAGGATAGAGAATATATGTTATACAAGTTTCAGGGGAAAGAT1900.6449204032449679No Hit
CAGAATGGTAATAAAACATGAATAATAAACGGTTGAGTTTTCTCATCCCAAG1880.6381317674213367No Hit
GGAAGGCTGGGGCTGTGTTTAAGTAAAGATACCAGATTCAGGTTGGCTGAAT1840.6245544957740742No Hit
ATAGTGGGTCGAAACAGAATAATAGAAAAATTCTTCTTTAATTTCCATTAAA1800.6109772241268117No Hit
CTCAATGGGAGATGTCGTGCACGGACTGTGTGAAAGAAGATGCTTTGCATAT1710.5804283629204712No Hit
CTTGCTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1680.5702454091850243No Hit
GCTAAAGGGATACATAAATACTACTAAAAAATTAATGAGCCTGGCAAACCAT1640.5566681375377618No Hit
ATGCCGGGACTTTTTGCATCAGTCACGTTAACCAGATGACTTTGCCTGTTAC1610.5464851838023149No Hit
TTGTCTGGGGGTTTTTTGTGGTGTACTCCGTGCCATCATGTCCGTCCTGACG1580.5363022300668681No Hit
CATAGAGGGGGTTGCTATAACTATATAACTTGACCAGTTGCAAACCGTCAAA1570.5329079121550524No Hit
CTGTTTGGGATCGGTCAGACAGCCACCATGTCACCAGATATGAACACATGTA1560.5295135942432369No Hit
GCAAACGGGGAGCTGGTTCGTACCAAGACCCTGGTGAAGAATTGCATCGTGC1520.5159363225959743No Hit
ATGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTT1490.5057533688605275No Hit
TGCTCTGGGGAGCACCGAAGACTGCGATGACTTCTGCGCTGACCCAGGGGCT1470.4989647330368962No Hit
CTACCAGGGACATTTGCCTACTAATGGAAAAGGTTAATAATTCATGCCAAAA1440.48878177930144934No Hit
ACACTCGGGCAAGTACCGAGGTTTTGAATAGTTAGGAATTAGATACTTTCAC1390.4718101897423712No Hit
ATAGCAGGGGTTGTTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCT1370.46502155391874006No Hit
GATTAAGGGGGAGTTTATCATTGAATAGAGTGCAGAGAAAGGACCGCAAGAA1360.4616272360069244No Hit
GTTGCGGGTAGGGATGCACGGGGGTGATCAAGACATGACTAGATGTGAACCT1320.44804996435966193No Hit
CCAGTGGGAGGTTCTCAAAGCAGGAACAGGGAGATGCAGCAGCTGCAGTTGG1320.44804996435966193No Hit
ATCGCAGGGCATTTATTATAGACAATAAAGGAACTATTGGACTAACAGTAGT1290.43786701062421507No Hit
CACGAGGGGAGCGGAGGTGGTGGCGGCGGAGGCTTTGGCAGCTCGGGACTGA1260.4276840568887682No Hit
AACGGTGGGGACAACCTGACTGTGAATTTGCAACCCATACACTGCGTGAATC1250.42428973897695255No Hit
ATGTGGGAGGATTCCTGTGGACTTTGAGATTCATGTTACCTCATCTTCTTTT1250.42428973897695255No Hit
GCGGCGGGGGCACCGTAACTTTCAAGCGCTCCTGTTGTTGTCGTTGTTTTAA1230.4175011031533214No Hit
CTTGCTGGGCTCGTGTAGGTATCTAGGGCTGCCTGTAGGTTCAGATGCTTGT1190.4039238315060588No Hit
ACAAGAGGGAGAAAAGAAAACATGTTCACATATATGAACACAGTAAATTAGA1180.40052951359424327No Hit
AACCCTGGGAATCATAACTCAAGGTAGCCTCGAATTCTTGGGCTCAAGACAT1170.39713519568242756No Hit
TGCTTGGGGGGTGTCTGAGATCTGGATCTGCCCATCACTTTGGCTAGTGACA1110.37676928821153394No Hit
ATTTCTGGGACATTTGTGAGCCACCACACCTGGCCAAGAATACCTTGTTAAA1090.3699806523879026No Hit
CTATAAGGGGGTAATAGACTGGTTTGACTATATTGTTAGCTGCCACAGTAAG1080.366586334476087No Hit
TCAGAAGGGCTCAGAAAAGGACCTTGTGAGACGGAGGAAGCGGCAGAAGGCG1080.366586334476087No Hit
ATATTGAGGGATCCTACTACTTTTAATCTGATTTTTCTTCAGGATTATTGAG1060.3597976986524558No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACG1040.35300906282882455No Hit
CGCAGGTGGGGGTCTCTAAGTTGATTTGTACCCAGTGGGTCAACTTCTGCAA1030.34961474491700895No Hit
GTCAATGGGAAGTTGACAGAGGTTCTGCTTGGGATAATGAAGAGCTGCCTGT1000.3394317911815621No Hit
GAGCACGGGGTACGTGGAGAAGATGGAGAACCAAATGAAAGGACTGGAGTCC980.3326431553579308No Hit
TGATAAGGGGTTGTAACATTGTGATTAGGATTTAAACTAATTCAGAGAATTG950.32246020162248396No Hit
CAGGCTGGAGTGCAGTGGCGTGAACTCGGCTTCACTGCAAGCTCCGCCACCT930.31567156579885275No Hit
AGTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTC930.31567156579885275No Hit
AAACCTTGGGGGAGGTGTAAGTTTCCAAGGGTCCTGGCTATGAACGAGAAAT930.31567156579885275No Hit
AACACAGGGGAAGAAAATAAAGTGAGGACTTAAAACAATTCATGAAAGTGGA890.3020942941515903No Hit
GATTCCGGGTAACTGAAATTTGCTGCTGATATGTTGAGTTTGTTCTTTAAAA880.2986999762397746No Hit
TTACTAGGGAGTGTCCTCCAGGCACTCAAACCTAGCCCTTCTGAAGCCGACC860.29191134041614336No Hit
GCGTATGGGAGGATGAAGAAGTTCGGCAAGGATTAAAAGCTTACTCAAATTG850.28851702250432776No Hit
TCAGATGGGAGGTGGGGTGGAGTCCCAGAGCTGCACAGGGTTTGGCCCAAGT850.28851702250432776No Hit
GAATAAGGGTTATGAATCAGCTAAACTTGTGTTTCCCCAAATATATTTGTTT850.28851702250432776No Hit
TCTCTGGGGGTTTAGCATTTGCCTCTAAACCCTGTTAGAAATATGTGAAGTT840.28512270459251216No Hit
TGTTCAGGGTGTGCATTCTGCAAGAAGACAATGAATCCATGTATTTCTATAT790.26815111503343403No Hit
TCTTGCGGGTGTGCATTCTGCAAGAAGACAATGAATCCATGTATTTCTATAT780.2647567971216184No Hit
TGATTTGGGGGTAGGCCAGCTGAGGCCCCAAGTGCCCAGGCTTGGTCACCGG760.25796816129798716No Hit
TTCTGTGGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAA740.25117952547435596No Hit
ACAAATGGGGCCCTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCC740.25117952547435596No Hit
TTTTTAGGCACTGGAGAGGTCTCCAAATGACATCCCAAGGACCTACCTCTGT710.2409965717389091No Hit
TTAAATGGGTAGTAGAAAATGTCTACTCCTCACTGCTGACATGTTAAACTTA710.2409965717389091No Hit
TATACTGGGATATATATATATATTTAAAAATATATCGGATAGTTGTATACAA690.23420793591527783No Hit
ACACACGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCT690.23420793591527783No Hit
GGGAAAGGGATGCTGAGAGCAATCTGAGTGGTCAAAATTAACTGTCCAGGAA660.22402498217983097No Hit
CTGTTAGGGAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGG650.22063066426801534No Hit
CGCAATGGGGAGAAACTCGCTGGCACCACAGTGGGCCCCTGGACCCGGCCGC640.2172363463561997No Hit
CAATCATATACCAAATCTCTCCCTCACTAAACGTAAGCCTTCTCCTCACTCT630.2138420284443841No Hit
TGCTATGGGGTTACAAGGTTCTTGGAAACAAAGTTGTATTGTCATTGGTGCC630.2138420284443841No Hit
TTTTGTGGGCAAGGATGGCGTGGTGGAGATCACCGGCAAGCACGAGGAGCGG620.21044771053256847No Hit
ATAGTAGGGATCTTGAAATGCCAAAAGGCATTTTAGTTTCTTTTCTCTGTGC610.20705339262075284No Hit
CATTGAGGGAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACC610.20705339262075284No Hit
GTAGGGGGGTGATACTCTTATGTACGAGATCTGTCTCTGCTGTTTAACTTCA600.20365907470893727No Hit
TTCTGAGGGCATTCCAGTGATAATGGTAGTCAGCCATGTGGCTGGATCATCG600.20365907470893727No Hit
TGCCGGGAAGAGGCCCTCTTACCGCTAGCGAGGTGATAGGACATCTGGCTTG590.20026475679712163No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGA570.19347612097349037No Hit
AAAAGCAGGGTGGGGGCCACCCCAATTATGCTGGGACCATTGTAAGTGGAGT550.18668748514985914No Hit
ACTTCCGGGGGATTCTGCCTCTTTAGTCCTCACTGTTAAATAAAACCCAATC540.1832931672380435No Hit
TAGACTGGGGATTCCATCTTCTATAGCTTTTTTAACAACATTGGAAAGGATC540.1832931672380435No Hit
AATAGGGGGGACTCTTACGCAGTCAAAAATCAGTATATAGCTTTTGACTCCT530.1798988493262279No Hit
TGTCAAGGGGAGTCTGAAGATGGCGGCCTCAGCAGCGCGAGGTGCTGCGGCG520.17650453141441227No Hit
CGTATAGGGTATGCACGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGAC520.17650453141441227No Hit
GTCGTGGGGGTTAAAGGGGACTTCAGCTTTTTATATAAACATCCACTTCTCT510.17311021350259664No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGATCCTGTCTCT500.16971589559078104No Hit
ACTTGGGGAGCTGGCTAGTGGCTTATTACTTGTGACTGGACCTCTGGTCCTC490.1663215776789654No Hit
CTAGCTGGGATTCATCCCTTTACCATACTGAAAATGTTTTGCTTAATTTCCA490.1663215776789654No Hit
CACTGTGGGGTGAGGTGTTTGCATGTGGCCATTACCGTCATTGGCCTGTGAA480.1629272597671498No Hit
GTTGAGGGCAAATGGTGGTAGTTGCTAGAATTAAAGACACGTTCTTGGTTCA470.15953294185533418No Hit
GAGGCGGGGCGTATCCTTATGGGGGAGCTCTCTAATCACCATGACCATCATG470.15953294185533418No Hit
GGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACT450.15274430603170291No Hit
CAGTTTGGGGATCTACTCTAGGGCACCATAGTCTAATAGAAAACAACCGAAA450.15274430603170291No Hit
ATTCTAGGGCAGTTAACAGAGCTCTAATAGAGTAAGTCAGTTGTAAATAATC420.14256135229625608No Hit
GCGACAGGGATAAATGCGGAGACGTAAAGCATTAATGCAAGTTAAAATGTTT410.13916703438444045No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGAACTCGTATGC410.13916703438444045No Hit
AGAACAGGGAGCGCAGAGCGGTTTGGTCGTTCGTTGGGCGGTGCTGGTTTTT400.13577271647262482No Hit
TGATCCGGGATATTTTTAAAAAAACAAAAGTAAGCCATCCTCAAATAAGGAA400.13577271647262482No Hit
ACTGCTGGGAGAATCCGAGGGGCCAATTTATGAAGGTCTCATCCTTTAAAAA400.13577271647262482No Hit
CGTTCGGGGGGCATGCCAGGGTTCGAGAAGAGTGAATGGCTTGACGTACTTG390.1323783985608092No Hit
GGCTTAGGGACTCCATGCTTTCATACTAATACACACTACACACACACAAATC380.12898408064899358No Hit
GCCACGCTGGACTGCCCACTGCATATAAATATATATATCTCTCTATTTTCAC380.12898408064899358No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATCACGATCCTGTCTCTTATAC370.12558976273717798No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGAT370.12558976273717798No Hit
ATTATGGGGTCTTCTAAAAGTTGCCAGTGCCACTTTTAAGAAGTGAATCACT370.12558976273717798No Hit
TATCCGGGATCATTGCTGCTACAGAAGTGCTTTAAGAGAATTGCCTGAAACA360.12219544482536233No Hit
AGGTATGGGGTCAGAGCGGTGAGCGAGGTGGGTTGGAGACTCAGCAGGCTCC350.11880112691354673No Hit
GTACACGGGGCGGAACCAGCCTGCACCCTCCCTTCTGGCCCAGCGACCTGGG330.11201249108991548No Hit
TAGTTTGGGGTTGACAATCGAGTAGTACTCCCAATTGAAGCCCCCATTCGTA320.10861817317809985No Hit
GCTTGAGGGGAATTCTAATACATAGGTGTATATTTGGTATTAGTTATTTTGA320.10861817317809985No Hit
ATCGCTGGGATGAATGTTAATTTTTTTTTAATTCCCTGCTAAAGACGTTTTC300.10182953735446863No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)