FastQCFastQC Report
Sat 31 Dec 2022
SRR5844342.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844342.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29206
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGC4481.5339313839621995No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGCCGTCT3511.201807847702527Illumina PCR Primer Index 1 (95% over 23bp)
TGTCGCGGTGGAGGAGATGCCCATGAATGTGGCTGACCTTATTTAATTCCTG3311.1333287680613573No Hit
GCATCTGGGTCGGGAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGAC2850.9758268848866671No Hit
TCTCTGGGAGTGTGTGAGAAAAGAATAAAAGTGAAATTTGCATAATGAATGT2550.8731082654249126No Hit
TGACGTGGGATAATAGAAAAAGTAATCATAAAATTTGAATGAAACCACAAAA2550.8731082654249126No Hit
AGCACAGGGAACATCTATCATGCTGGAGGACTACTGTGATGGGGCTTTACAT2530.8662603574607958No Hit
GAACACGGGGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC2480.8491405875505034No Hit
CATATGGGGCTACTTGGGAATGCCCCAACTAAATACTACCGTATGGCCCACC2440.8354447716222693No Hit
GCAGCCGGGCCTTATGAACACTGATGTAAAAATTTTCAACAGAATACTAGCA2420.8285968636581524No Hit
TGTTTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2390.8183250017119771No Hit
AGTATTGGGGGCGGCCCTCGCGGCAGGTTTCGGGCTTCAGGACAATTCGTGA2370.81147709374786No Hit
GACGAAGGGTGAAAGATAGAGATTAATACAACTACTTAAAAAATATAGTCAA2290.7840854618913922No Hit
TTAGAAGGGTGGTTCATTTGTAAATTATTATTCATATAGACCACTGTAGTAG2250.7703896459631583No Hit
GGTAACTGGCACACTTCCCAAGCTCAAGAGCTCAAAAGAATGGTTGGAGCCC2240.7669656919810998No Hit
AAACATGGGGCTCCGACCCGGAACTGCTTTCTTGGGAAGATGGCGGCTGTGT2220.7601177840169828No Hit
GGCAAGGAGTTCCAGCGCCGCGCCAAGGCCATGATGCTGCGGGCAGCTGTGC2190.7498459220708074No Hit
GTATATGGGGAGAGTGAGGATGAAAGCAATGAAGAGGAGGAAGAGGAAGATG2170.7429980141066904No Hit
CACCGTGGGGTGAGGCCAAATATCATTCTAAGGGGCCACAGTAATTACAAAC2170.7429980141066904No Hit
GTTACAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC2120.725878244196398No Hit
TGTAATGGGACTAAGTAAGATCACAGATAGCATACAAATTCACTGACCATTA2030.6950626583578716No Hit
GCATACGGGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGC2010.6882147503937547No Hit
TAACTAGGGCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTC1990.6813668424296377No Hit
TCTGAAGGGTCAGGATAGAGAATATATGTTATACAAGTTTCAGGGGAAAGAT1980.6779428884475792No Hit
GCAGGCGGGCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTA1970.6745189344655208No Hit
ACGCCTGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1900.6505512565911113No Hit
GGAAGGCTGGGGCTGTGTTTAAGTAAAGATACCAGATTCAGGTTGGCTGAAT1880.6437033486269944No Hit
CTTGCTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1820.6231596247346436No Hit
GTTATGGGGGTTCATTACCTCCTCCTACCCCAAGAGAAATGGATTCAGACAT1820.6231596247346436No Hit
ATAAGTGGGATGGTGGTCATACTGCCAAGATATCTGATTTCTCCTGGAATCC1810.6197356707525851No Hit
TAGGGTGGGGGCCCAGCGCAGATCGCTTGCAGCTTGCTAGCTGTGTGGGCTG1790.6128877627884681No Hit
ATAGTGGGTCGAAACAGAATAATAGAAAAATTCTTCTTTAATTTCCATTAAA1790.6128877627884681No Hit
CTGTTTGGGATCGGTCAGACAGCCACCATGTCACCAGATATGAACACATGTA1780.6094638088064096No Hit
ATCGCAGGGCATTTATTATAGACAATAAAGGAACTATTGGACTAACAGTAGT1740.5957679928781757No Hit
CTTCCTGGGACTGGCGGGGAGCGCGTAACGAGAGACTGCTTGCTGCGGCAGA1730.5923440388961173No Hit
ATGCCGGGACTTTTTGCATCAGTCACGTTAACCAGATGACTTTGCCTGTTAC1710.5854961309320003No Hit
CTCAATGGGAGATGTCGTGCACGGACTGTGTGAAAGAAGATGCTTTGCATAT1660.5683763610217079No Hit
CAGAATGGTAATAAAACATGAATAATAAACGGTTGAGTTTTCTCATCCCAAG1650.5649524070396493No Hit
ATGTGGGAGGATTCCTGTGGACTTTGAGATTCATGTTACCTCATCTTCTTTT1640.5615284530575909No Hit
ATGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTT1610.5512565911114155No Hit
AAATTAGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1600.547832637129357No Hit
TTGTCTGGGGGTTTTTTGTGGTGTACTCCGTGCCATCATGTCCGTCCTGACG1540.5272889132370061No Hit
GATTAAGGGGGAGTTTATCATTGAATAGAGTGCAGAGAAAGGACCGCAAGAA1520.5204410052728892No Hit
AACCCTGGGAATCATAACTCAAGGTAGCCTCGAATTCTTGGGCTCAAGACAT1510.5170170512908306No Hit
GCTAAAGGGATACATAAATACTACTAAAAAATTAATGAGCCTGGCAAACCAT1500.5135930973087722No Hit
AACGGTGGGGACAACCTGACTGTGAATTTGCAACCCATACACTGCGTGAATC1430.48962541943436283No Hit
ACAAGAGGGAGAAAAGAAAACATGTTCACATATATGAACACAGTAAATTAGA1430.48962541943436283No Hit
ATAGCAGGGGTTGTTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCT1400.47935355748818737No Hit
CATAGAGGGGGTTGCTATAACTATATAACTTGACCAGTTGCAAACCGTCAAA1390.47592960350612884No Hit
TGCTCTGGGGAGCACCGAAGACTGCGATGACTTCTGCGCTGACCCAGGGGCT1320.4519619256317195No Hit
GCAAACGGGGAGCTGGTTCGTACCAAGACCCTGGTGAAGAATTGCATCGTGC1290.44169006368554403No Hit
CTTGCTGGGCTCGTGTAGGTATCTAGGGCTGCCTGTAGGTTCAGATGCTTGT1240.4245702937752517No Hit
CTACCAGGGACATTTGCCTACTAATGGAAAAGGTTAATAATTCATGCCAAAA1220.41772238581113463No Hit
GTTGCGGGTAGGGATGCACGGGGGTGATCAAGACATGACTAGATGTGAACCT1210.4142984318290762No Hit
ATATTGAGGGATCCTACTACTTTTAATCTGATTTTTCTTCAGGATTATTGAG1190.4074505238649592No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACG1150.39375470793672535No Hit
CACGAGGGGAGCGGAGGTGGTGGCGGCGGAGGCTTTGGCAGCTCGGGACTGA1120.3834828459905499No Hit
CCAGTGGGAGGTTCTCAAAGCAGGAACAGGGAGATGCAGCAGCTGCAGTTGG1120.3834828459905499No Hit
TTACTAGGGAGTGTCCTCCAGGCACTCAAACCTAGCCCTTCTGAAGCCGACC1120.3834828459905499No Hit
CGCAGGTGGGGGTCTCTAAGTTGATTTGTACCCAGTGGGTCAACTTCTGCAA1110.3800588920084914No Hit
ATTTCTGGGACATTTGTGAGCCACCACACCTGGCCAAGAATACCTTGTTAAA1100.3766349380264329No Hit
AACACAGGGGAAGAAAATAAAGTGAGGACTTAAAACAATTCATGAAAGTGGA1100.3766349380264329No Hit
TGATTTGGGGGTAGGCCAGCTGAGGCCCCAAGTGCCCAGGCTTGGTCACCGG1080.36978703006231595No Hit
ACACTCGGGCAAGTACCGAGGTTTTGAATAGTTAGGAATTAGATACTTTCAC1070.3663630760802575No Hit
GTCAATGGGAAGTTGACAGAGGTTCTGCTTGGGATAATGAAGAGCTGCCTGT980.33554749024173114No Hit
CTATAAGGGGGTAATAGACTGGTTTGACTATATTGTTAGCTGCCACAGTAAG970.33212353625967267No Hit
TGTTCAGGGTGTGCATTCTGCAAGAAGACAATGAATCCATGTATTTCTATAT940.3218516743134972No Hit
TCAGATGGGAGGTGGGGTGGAGTCCCAGAGCTGCACAGGGTTTGGCCCAAGT930.31842772033143874No Hit
GCGGCGGGGGCACCGTAACTTTCAAGCGCTCCTGTTGTTGTCGTTGTTTTAA930.31842772033143874No Hit
GAATAAGGGTTATGAATCAGCTAAACTTGTGTTTCCCCAAATATATTTGTTT930.31842772033143874No Hit
TCAGAAGGGCTCAGAAAAGGACCTTGTGAGACGGAGGAAGCGGCAGAAGGCG920.31500376634938027No Hit
CTGTTAGGGAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGG890.3047319044032048No Hit
CAGGCTGGAGTGCAGTGGCGTGAACTCGGCTTCACTGCAAGCTCCGCCACCT870.29788399643908786No Hit
GATTCCGGGTAACTGAAATTTGCTGCTGATATGTTGAGTTTGTTCTTTAAAA860.2944600424570294No Hit
TGCTTGGGGGGTGTCTGAGATCTGGATCTGCCCATCACTTTGGCTAGTGACA850.2910360884749709No Hit
ACAAATGGGGCCCTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCC850.2910360884749709No Hit
TGATAAGGGGTTGTAACATTGTGATTAGGATTTAAACTAATTCAGAGAATTG850.2910360884749709No Hit
GAGCACGGGGTACGTGGAGAAGATGGAGAACCAAATGAAAGGACTGGAGTCC840.2876121344929124No Hit
AGTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTC830.2841881805108539No Hit
TCTCTGGGGGTTTAGCATTTGCCTCTAAACCCTGTTAGAAATATGTGAAGTT810.277340272546737No Hit
TTAAATGGGTAGTAGAAAATGTCTACTCCTCACTGCTGACATGTTAAACTTA800.2739163185646785No Hit
CATTGAGGGAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACC790.27049236458262No Hit
TGCTATGGGGTTACAAGGTTCTTGGAAACAAAGTTGTATTGTCATTGGTGCC760.2602205026364446No Hit
AAAAGCAGGGTGGGGGCCACCCCAATTATGCTGGGACCATTGTAAGTGGAGT750.2567965486543861No Hit
AAACCTTGGGGGAGGTGTAAGTTTCCAAGGGTCCTGGCTATGAACGAGAAAT740.2533725946723276No Hit
GCGTATGGGAGGATGAAGAAGTTCGGCAAGGATTAAAAGCTTACTCAAATTG720.24652468670821065No Hit
TTCTGTGGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAA720.24652468670821065No Hit
ATAGTAGGGATCTTGAAATGCCAAAAGGCATTTTAGTTTCTTTTCTCTGTGC720.24652468670821065No Hit
AGAACAGGGAGCGCAGAGCGGTTTGGTCGTTCGTTGGGCGGTGCTGGTTTTT690.2362528247620352No Hit
TTTTGTGGGCAAGGATGGCGTGGTGGAGATCACCGGCAAGCACGAGGAGCGG690.2362528247620352No Hit
TCTTGCGGGTGTGCATTCTGCAAGAAGACAATGAATCCATGTATTTCTATAT660.22598096281585975No Hit
TATACTGGGATATATATATATATTTAAAAATATATCGGATAGTTGTATACAA660.22598096281585975No Hit
CAATCATATACCAAATCTCTCCCTCACTAAACGTAAGCCTTCTCCTCACTCT650.22255700883380128No Hit
ACACACGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCT580.1985893309593919No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGATCCTGTCTCT560.19174142299527494No Hit
CGTATAGGGTATGCACGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGAC560.19174142299527494No Hit
TGTCAAGGGGAGTCTGAAGATGGCGGCCTCAGCAGCGCGAGGTGCTGCGGCG550.18831746901321644No Hit
CGCAATGGGGAGAAACTCGCTGGCACCACAGTGGGCCCCTGGACCCGGCCGC550.18831746901321644No Hit
CACTGTGGGGTGAGGTGTTTGCATGTGGCCATTACCGTCATTGGCCTGTGAA530.1814695610490995No Hit
TTCTGAGGGCATTCCAGTGATAATGGTAGTCAGCCATGTGGCTGGATCATCG510.17462165308498254No Hit
ATTATGGGGTCTTCTAAAAGTTGCCAGTGCCACTTTTAAGAAGTGAATCACT500.17119769910292404No Hit
TTTTTAGGCACTGGAGAGGTCTCCAAATGACATCCCAAGGACCTACCTCTGT490.16777374512086557No Hit
GGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACT490.16777374512086557No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGA480.1643497911388071No Hit
GTTGAGGGCAAATGGTGGTAGTTGCTAGAATTAAAGACACGTTCTTGGTTCA480.1643497911388071No Hit
TGCCGGGAAGAGGCCCTCTTACCGCTAGCGAGGTGATAGGACATCTGGCTTG480.1643497911388071No Hit
ACTTCCGGGGGATTCTGCCTCTTTAGTCCTCACTGTTAAATAAAACCCAATC450.15407792919263166No Hit
CAGTTTGGGGATCTACTCTAGGGCACCATAGTCTAATAGAAAACAACCGAAA450.15407792919263166No Hit
CTAGCTGGGATTCATCCCTTTACCATACTGAAAATGTTTTGCTTAATTTCCA430.1472300212285147No Hit
GTAGGGGGGTGATACTCTTATGTACGAGATCTGTCTCTGCTGTTTAACTTCA420.1438060672464562No Hit
ATTCTAGGGCAGTTAACAGAGCTCTAATAGAGTAAGTCAGTTGTAAATAATC420.1438060672464562No Hit
TAGACTGGGGATTCCATCTTCTATAGCTTTTTTAACAACATTGGAAAGGATC410.14038211326439773No Hit
AATAGGGGGGACTCTTACGCAGTCAAAAATCAGTATATAGCTTTTGACTCCT410.14038211326439773No Hit
GTCGTGGGGGTTAAAGGGGACTTCAGCTTTTTATATAAACATCCACTTCTCT390.13353420530028076No Hit
GGCTTAGGGACTCCATGCTTTCATACTAATACACACTACACACACACAAATC390.13353420530028076No Hit
GGGAAAGGGATGCTGAGAGCAATCTGAGTGGTCAAAATTAACTGTCCAGGAA390.13353420530028076No Hit
CGAGCTGGGTCCTTTCTCCAGTCCACGTGTAGACTTTGCGCTTGATGAAGAA390.13353420530028076No Hit
CGTTCGGGGGGCATGCCAGGGTTCGAGAAGAGTGAATGGCTTGACGTACTTG360.12326234335410532No Hit
ACTTGGGGAGCTGGCTAGTGGCTTATTACTTGTGACTGGACCTCTGGTCCTC350.11983838937204684No Hit
GAGGCGGGGCGTATCCTTATGGGGGAGCTCTCTAATCACCATGACCATCATG350.11983838937204684No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGAACTCGTATGC350.11983838937204684No Hit
GTTAATGGGTAACCTGCTCGTTAAATAATGTGTTGGTGTGAGATATCAGGAA350.11983838937204684No Hit
ACTGCTGGGAGAATCCGAGGGGCCAATTTATGAAGGTCTCATCCTTTAAAAA350.11983838937204684No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATCACGATCCTGTCTCTTATAC340.11641443538998836No Hit
TATCCGGGATCATTGCTGCTACAGAAGTGCTTTAAGAGAATTGCCTGAAACA340.11641443538998836No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGCC320.1095665274258714No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGAT320.1095665274258714No Hit
GCGACAGGGATAAATGCGGAGACGTAAAGCATTAATGCAAGTTAAAATGTTT310.10614257344381292No Hit
GTGTAAGGGCTGTTTCAAACTAAAGCTCCCACCTCGAACATTGACCCTTTGT310.10614257344381292No Hit
TACTAAGGGATGATTTCCAGGCTCCATATCTTCTACATGGAAAATATGTCAA300.10271861946175444No Hit
TGATCCGGGATATTTTTAAAAAAACAAAAGTAAGCCATCCTCAAATAAGGAA300.10271861946175444No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)