FastQCFastQC Report
Sat 31 Dec 2022
SRR5844341.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844341.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29190
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGC4311.4765330592668722No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATCACGATCTCGTATGCCGTCT3311.1339499828708461Illumina PCR Primer Index 1 (95% over 23bp)
TGTCGCGGTGGAGGAGATGCCCATGAATGTGGCTGACCTTATTTAATTCCTG2910.9969167523124357No Hit
AGCACAGGGAACATCTATCATGCTGGAGGACTACTGTGATGGGGCTTTACAT2790.9558067831449126No Hit
GCATCTGGGTCGGGAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGAC2690.9215484755053102No Hit
GAACACGGGGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC2630.9009934909215485No Hit
GACGAAGGGTGAAAGATAGAGATTAATACAACTACTTAAAAAATATAGTCAA2560.8770126755738267No Hit
TGTTTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2510.8598835217540254No Hit
TCTCTGGGAGTGTGTGAGAAAAGAATAAAAGTGAAATTTGCATAATGAATGT2390.8187735525865022No Hit
TTAGAAGGGTGGTTCATTTGTAAATTATTATTCATATAGACCACTGTAGTAG2360.8084960602946214No Hit
AAACATGGGGCTCCGACCCGGAACTGCTTTCTTGGGAAGATGGCGGCTGTGT2310.7913669064748201No Hit
ATAAGTGGGATGGTGGTCATACTGCCAAGATATCTGATTTCTCCTGGAATCC2280.7810894141829393No Hit
CATATGGGGCTACTTGGGAATGCCCCAACTAAATACTACCGTATGGCCCACC2280.7810894141829393No Hit
GCAGCCGGGCCTTATGAACACTGATGTAAAAATTTTCAACAGAATACTAGCA2280.7810894141829393No Hit
GCATACGGGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGC2280.7810894141829393No Hit
GTATATGGGGAGAGTGAGGATGAAAGCAATGAAGAGGAGGAAGAGGAAGATG2210.7571085988352175No Hit
TGTAATGGGACTAAGTAAGATCACAGATAGCATACAAATTCACTGACCATTA2180.7468311065433368No Hit
TAACTAGGGCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTC2170.7434052757793765No Hit
CTTGCTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC2120.7262761219595751No Hit
CACCGTGGGGTGAGGCCAAATATCATTCTAAGGGGCCACAGTAATTACAAAC2120.7262761219595751No Hit
GGCAAGGAGTTCCAGCGCCGCGCCAAGGCCATGATGCTGCGGGCAGCTGTGC2110.722850291195615No Hit
GGAAGGCTGGGGCTGTGTTTAAGTAAAGATACCAGATTCAGGTTGGCTGAAT2080.7125727989037342No Hit
AGTATTGGGGGCGGCCCTCGCGGCAGGTTTCGGGCTTCAGGACAATTCGTGA2080.7125727989037342No Hit
CAGAATGGTAATAAAACATGAATAATAAACGGTTGAGTTTTCTCATCCCAAG2030.6954436450839329No Hit
GCAGGCGGGCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTA2000.6851661527920521No Hit
GTTACAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1980.6783144912641316No Hit
TTGTCTGGGGGTTTTTTGTGGTGTACTCCGTGCCATCATGTCCGTCCTGACG1940.6646111682082906No Hit
ACGCCTGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1910.6543336759164098No Hit
GTTATGGGGGTTCATTACCTCCTCCTACCCCAAGAGAAATGGATTCAGACAT1900.6509078451524495No Hit
TGACGTGGGATAATAGAAAAAGTAATCATAAAATTTGAATGAAACCACAAAA1890.6474820143884892No Hit
ATAGTGGGTCGAAACAGAATAATAGAAAAATTCTTCTTTAATTTCCATTAAA1890.6474820143884892No Hit
AAATTAGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1870.6406303528605687No Hit
GGTAACTGGCACACTTCCCAAGCTCAAGAGCTCAAAAGAATGGTTGGAGCCC1810.6200753682768071No Hit
CATAGAGGGGGTTGCTATAACTATATAACTTGACCAGTTGCAAACCGTCAAA1710.5858170606372045No Hit
TAGGGTGGGGGCCCAGCGCAGATCGCTTGCAGCTTGCTAGCTGTGTGGGCTG1680.5755395683453237No Hit
ACAAGAGGGAGAAAAGAAAACATGTTCACATATATGAACACAGTAAATTAGA1650.5652620760534429No Hit
CTACCAGGGACATTTGCCTACTAATGGAAAAGGTTAATAATTCATGCCAAAA1630.5584104145255224No Hit
ATGCCGGGACTTTTTGCATCAGTCACGTTAACCAGATGACTTTGCCTGTTAC1620.5549845837615622No Hit
GATTAAGGGGGAGTTTATCATTGAATAGAGTGCAGAGAAAGGACCGCAAGAA1600.5481329222336416No Hit
CTTCCTGGGACTGGCGGGGAGCGCGTAACGAGAGACTGCTTGCTGCGGCAGA1580.5412812607057211No Hit
TCTGAAGGGTCAGGATAGAGAATATATGTTATACAAGTTTCAGGGGAAAGAT1570.5378554299417608No Hit
ATGTGGGAGGATTCCTGTGGACTTTGAGATTCATGTTACCTCATCTTCTTTT1510.5173004453579994No Hit
TGCTCTGGGGAGCACCGAAGACTGCGATGACTTCTGCGCTGACCCAGGGGCT1490.5104487838300787No Hit
ATAGCAGGGGTTGTTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCT1490.5104487838300787No Hit
CACGAGGGGAGCGGAGGTGGTGGCGGCGGAGGCTTTGGCAGCTCGGGACTGA1450.49674546077423776No Hit
CTGTTTGGGATCGGTCAGACAGCCACCATGTCACCAGATATGAACACATGTA1410.4830421377183967No Hit
GCTAAAGGGATACATAAATACTACTAAAAAATTAATGAGCCTGGCAAACCAT1400.4796163069544364No Hit
GCAAACGGGGAGCTGGTTCGTACCAAGACCCTGGTGAAGAATTGCATCGTGC1400.4796163069544364No Hit
ATATTGAGGGATCCTACTACTTTTAATCTGATTTTTCTTCAGGATTATTGAG1370.46933881466255567No Hit
ATCGCAGGGCATTTATTATAGACAATAAAGGAACTATTGGACTAACAGTAGT1350.4624871531346352No Hit
CCAGTGGGAGGTTCTCAAAGCAGGAACAGGGAGATGCAGCAGCTGCAGTTGG1330.4556354916067146No Hit
ATTTCTGGGACATTTGTGAGCCACCACACCTGGCCAAGAATACCTTGTTAAA1320.4522096608427543No Hit
ATGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTT1310.44878383007879413No Hit
CTTGCTGGGCTCGTGTAGGTATCTAGGGCTGCCTGTAGGTTCAGATGCTTGT1300.4453579993148339No Hit
GTTGCGGGTAGGGATGCACGGGGGTGATCAAGACATGACTAGATGTGAACCT1260.4316546762589928No Hit
TCAGATGGGAGGTGGGGTGGAGTCCCAGAGCTGCACAGGGTTTGGCCCAAGT1230.42137718396711205No Hit
AACCCTGGGAATCATAACTCAAGGTAGCCTCGAATTCTTGGGCTCAAGACAT1210.4145255224391915No Hit
CTCAATGGGAGATGTCGTGCACGGACTGTGTGAAAGAAGATGCTTTGCATAT1200.41109969167523125No Hit
CTATAAGGGGGTAATAGACTGGTTTGACTATATTGTTAGCTGCCACAGTAAG1180.4042480301473107No Hit
AACGGTGGGGACAACCTGACTGTGAATTTGCAACCCATACACTGCGTGAATC1160.39739636861939015No Hit
GAATAAGGGTTATGAATCAGCTAAACTTGTGTTTCCCCAAATATATTTGTTT1160.39739636861939015No Hit
CGCAGGTGGGGGTCTCTAAGTTGATTTGTACCCAGTGGGTCAACTTCTGCAA1150.39397053785542996No Hit
TCTCTGGGGGTTTAGCATTTGCCTCTAAACCCTGTTAGAAATATGTGAAGTT1140.39054470709146966No Hit
ACACTCGGGCAAGTACCGAGGTTTTGAATAGTTAGGAATTAGATACTTTCAC1140.39054470709146966No Hit
GCGGCGGGGGCACCGTAACTTTCAAGCGCTCCTGTTGTTGTCGTTGTTTTAA1090.3734155532716684No Hit
TGTTCAGGGTGTGCATTCTGCAAGAAGACAATGAATCCATGTATTTCTATAT1040.3562863994518671No Hit
TTACTAGGGAGTGTCCTCCAGGCACTCAAACCTAGCCCTTCTGAAGCCGACC1040.3562863994518671No Hit
TGCTTGGGGGGTGTCTGAGATCTGGATCTGCCCATCACTTTGGCTAGTGACA1020.3494347379239465No Hit
AACACAGGGGAAGAAAATAAAGTGAGGACTTAAAACAATTCATGAAAGTGGA1020.3494347379239465No Hit
CAGGCTGGAGTGCAGTGGCGTGAACTCGGCTTCACTGCAAGCTCCGCCACCT1000.34258307639602603No Hit
GATTCCGGGTAACTGAAATTTGCTGCTGATATGTTGAGTTTGTTCTTTAAAA980.3357314148681055No Hit
CATTGAGGGAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACC980.3357314148681055No Hit
ACAAATGGGGCCCTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCC970.3323055841041453No Hit
GTCAATGGGAAGTTGACAGAGGTTCTGCTTGGGATAATGAAGAGCTGCCTGT970.3323055841041453No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACG940.32202809181226444No Hit
GCGTATGGGAGGATGAAGAAGTTCGGCAAGGATTAAAAGCTTACTCAAATTG910.3117505995203837No Hit
AGTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTC910.3117505995203837No Hit
TCAGAAGGGCTCAGAAAAGGACCTTGTGAGACGGAGGAAGCGGCAGAAGGCG910.3117505995203837No Hit
AAACCTTGGGGGAGGTGTAAGTTTCCAAGGGTCCTGGCTATGAACGAGAAAT900.3083247687564234No Hit
TGATTTGGGGGTAGGCCAGCTGAGGCCCCAAGTGCCCAGGCTTGGTCACCGG880.3014731072285029No Hit
TTAAATGGGTAGTAGAAAATGTCTACTCCTCACTGCTGACATGTTAAACTTA880.3014731072285029No Hit
TGATAAGGGGTTGTAACATTGTGATTAGGATTTAAACTAATTCAGAGAATTG880.3014731072285029No Hit
TCTTGCGGGTGTGCATTCTGCAAGAAGACAATGAATCCATGTATTTCTATAT840.28776978417266186No Hit
CTGTTAGGGAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGG780.26721479958890026No Hit
TTCTGAGGGCATTCCAGTGATAATGGTAGTCAGCCATGTGGCTGGATCATCG780.26721479958890026No Hit
TTTTGTGGGCAAGGATGGCGTGGTGGAGATCACCGGCAAGCACGAGGAGCGG770.2637889688249401No Hit
CAATCATATACCAAATCTCTCCCTCACTAAACGTAAGCCTTCTCCTCACTCT770.2637889688249401No Hit
TGCCGGGAAGAGGCCCTCTTACCGCTAGCGAGGTGATAGGACATCTGGCTTG740.2535114765330593No Hit
CAGTTTGGGGATCTACTCTAGGGCACCATAGTCTAATAGAAAACAACCGAAA720.24665981500513876No Hit
GAGCACGGGGTACGTGGAGAAGATGGAGAACCAAATGAAAGGACTGGAGTCC700.2398081534772182No Hit
TATACTGGGATATATATATATATTTAAAAATATATCGGATAGTTGTATACAA700.2398081534772182No Hit
TGCTATGGGGTTACAAGGTTCTTGGAAACAAAGTTGTATTGTCATTGGTGCC690.236382322713258No Hit
ACACACGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCT680.2329564919492977No Hit
AATAGGGGGGACTCTTACGCAGTCAAAAATCAGTATATAGCTTTTGACTCCT670.22953066118533744No Hit
GGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACT650.22267899965741694No Hit
ATAGTAGGGATCTTGAAATGCCAAAAGGCATTTTAGTTTCTTTTCTCTGTGC640.21925316889345667No Hit
TAGACTGGGGATTCCATCTTCTATAGCTTTTTTAACAACATTGGAAAGGATC590.20212401507365535No Hit
TGTCAAGGGGAGTCTGAAGATGGCGGCCTCAGCAGCGCGAGGTGCTGCGGCG580.19869818430969508No Hit
AAAAGCAGGGTGGGGGCCACCCCAATTATGCTGGGACCATTGTAAGTGGAGT580.19869818430969508No Hit
CGCAATGGGGAGAAACTCGCTGGCACCACAGTGGGCCCCTGGACCCGGCCGC580.19869818430969508No Hit
GTAGGGGGGTGATACTCTTATGTACGAGATCTGTCTCTGCTGTTTAACTTCA550.1884206920178143No Hit
CGTTCGGGGGGCATGCCAGGGTTCGAGAAGAGTGAATGGCTTGACGTACTTG530.18156903048989378No Hit
CACTGTGGGGTGAGGTGTTTGCATGTGGCCATTACCGTCATTGGCCTGTGAA510.17471736896197326No Hit
TTCTGTGGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAA500.17129153819801302No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGATCCTGTCTCT490.16786570743405274No Hit
CGTATAGGGTATGCACGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGAC490.16786570743405274No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGA480.1644398766700925No Hit
ATTATGGGGTCTTCTAAAAGTTGCCAGTGCCACTTTTAAGAAGTGAATCACT480.1644398766700925No Hit
GAGGCGGGGCGTATCCTTATGGGGGAGCTCTCTAATCACCATGACCATCATG470.16101404590613222No Hit
GTTGAGGGCAAATGGTGGTAGTTGCTAGAATTAAAGACACGTTCTTGGTTCA460.15758821514217197No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAATCACGAACTCGTATGC460.15758821514217197No Hit
AGAACAGGGAGCGCAGAGCGGTTTGGTCGTTCGTTGGGCGGTGCTGGTTTTT450.1541623843782117No Hit
GTGTAAGGGCTGTTTCAAACTAAAGCTCCCACCTCGAACATTGACCCTTTGT450.1541623843782117No Hit
TACATGGGACTGCCCTAAGTCAAGAGATTAATGGAAATTGAGAGTGTTCTCA440.15073655361425145No Hit
TTTTTAGGCACTGGAGAGGTCTCCAAATGACATCCCAAGGACCTACCTCTGT430.14731072285029118No Hit
ATTCTAGGGCAGTTAACAGAGCTCTAATAGAGTAAGTCAGTTGTAAATAATC430.14731072285029118No Hit
ACTTGGGGAGCTGGCTAGTGGCTTATTACTTGTGACTGGACCTCTGGTCCTC420.14388489208633093No Hit
GGGAAAGGGATGCTGAGAGCAATCTGAGTGGTCAAAATTAACTGTCCAGGAA420.14388489208633093No Hit
ACTTCCGGGGGATTCTGCCTCTTTAGTCCTCACTGTTAAATAAAACCCAATC410.14045906132237065No Hit
TATCCGGGATCATTGCTGCTACAGAAGTGCTTTAAGAGAATTGCCTGAAACA410.14045906132237065No Hit
GTCGTGGGGGTTAAAGGGGACTTCAGCTTTTTATATAAACATCCACTTCTCT400.1370332305584104No Hit
ACTGCTGGGAGAATCCGAGGGGCCAATTTATGAAGGTCTCATCCTTTAAAAA400.1370332305584104No Hit
CTAGCTGGGATTCATCCCTTTACCATACTGAAAATGTTTTGCTTAATTTCCA400.1370332305584104No Hit
TAGTTTGGGGTTGACAATCGAGTAGTACTCCCAATTGAAGCCCCCATTCGTA370.12675573826652964No Hit
TGATCCGGGATATTTTTAAAAAAACAAAAGTAAGCCATCCTCAAATAAGGAA370.12675573826652964No Hit
GTTAATGGGTAACCTGCTCGTTAAATAATGTGTTGGTGTGAGATATCAGGAA370.12675573826652964No Hit
ATCGCTGGGATGAATGTTAATTTTTTTTTAATTCCCTGCTAAAGACGTTTTC370.12675573826652964No Hit
GCTTGAGGGGAATTCTAATACATAGGTGTATATTTGGTATTAGTTATTTTGA370.12675573826652964No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATCACGATCCTGTCTCTTATAC360.12332990750256938No Hit
GCGACAGGGATAAATGCGGAGACGTAAAGCATTAATGCAAGTTAAAATGTTT360.12332990750256938No Hit
GGCTTAGGGACTCCATGCTTTCATACTAATACACACTACACACACACAAATC330.11305241521068858No Hit
GTACACGGGGCGGAACCAGCCTGCACCCTCCCTTCTGGCCCAGCGACCTGGG310.10620075368276806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)