FastQCFastQC Report
Sat 31 Dec 2022
SRR5844301.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844301.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24612
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGTGGGGTTCAAGGTGATAAAGTTTTTCTAGTAAGGCTCCCCTCG2280.9263773768893223No Hit
CCGACTGGGCATACAACAATTGAAAGTGTTTGCTCATGAAATCAGTTATAAA2150.8735576141719487No Hit
ATTAGTGGGGTGTTCCTTGGCTTAAAGCCCCTTCCTCCATCTGCAAAGCCAG2130.8654314968308142No Hit
ACTTTTGGGAAGAAAATAGTGGTGTGATTTGGGGATATTTTTTTCTTTTCTT2110.8573053794896799No Hit
AAGGCTGGGCTCTGCCGGTGCAGAATATGAACAATGCCATGTTCTTGCAGAA2100.8532423208191127No Hit
TTATCTGGGATACTCTTCAATCAGCCACATAGCCCTCGTAGTAACAGCCATT2080.8451162034779781No Hit
AGTTACGGGCTCCAAGACTTCATCAATATTAAACTCAATTCAGCTTCTATCT2070.841053144807411No Hit
TTCACTGGGAGATTATTTCTGTAGTTATATTTCTCCACTCTTTCTCGAATTT2030.8248009101251422No Hit
TCAATTGGGATCAGCTCAAGTTGTACTCAGAGAAGCAGAGGAACTGGGAGGA1950.7922964407606046No Hit
TTAAAGGGGTGCTTCTTTCTTTGGGAATATTTGAATCTTGTCTCAATGCTCA1870.759791971396067No Hit
CATAGGGGGGCAAATAGGGAGATTATCCTGAATTATCTCTTAGAATCCAAGG1820.7394766780432309No Hit
TATTAGGGGACAGTTTCTTGTTTCGAGTGCTGACTGACCCGTTTCTGAAATG1810.7354136193726637No Hit
TAGCTTGGGAGTGTGTAAGCTAAACAAAAACTAAAACTAAGAATTCTCAAAA1790.7272875020315294No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGTATGC1740.7069722086786934No Hit
CTTCAAGAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC1680.6825938566552902No Hit
CGTTGAGGGGACTGAAGAAACCCGCCGGCTGCTGGGGCAGGGCGGCGAAAGG1670.6785307979847229No Hit
GACAGGGAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGA1620.6582155046318869No Hit
TGATATGGGGGGGACGGACCGGAAGTGCCCGAGGGCGGCCGCAGAACGGTCA1500.6094588005850805No Hit
CTTATGGGGTACTTATTTTATTGATTCAGTTTTGCTAATTTTGCTAGTAGGA1460.5932065659028116No Hit
GAAACAGGGCATCATGAACTTGGACCAAAATGAATTCGCTGGCTTCTCTTAT1440.5850804485616773No Hit
AAGCCAGGGACCTACCCAGTATTGTGTCAAGCTTGAACAATAAAATAGAGTT1410.5728912725499756No Hit
GTCAATGGGAATGCTTTTTTGTTTTTGCTTTTTCTAACAAAACAATCTTTTT1370.5566390378677069No Hit
ATTCATGGGGCAGGTGCTCCTCGATGTCCTTGCGGTCGTAGGTGATGCCACT1360.5525759791971396No Hit
TCACAGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1350.5485129205265724No Hit
ATACCTGGGGTTATGACTTTGATGTGCATACAATGAAGACACTAAAAAACAT1340.5444498618560052No Hit
AGCCTAGGGTCTGTCGTTGGGCTTTGGTATGATGTTAGCCATGGGTTTGTCA1340.5444498618560052No Hit
AGTCAAGGGTGGGAATGATGATTTCACAGACTAGAGTCTCCGATGCTGGTCA1330.5403868031854381No Hit
TGTGCTGGCATGAATATTCTAAAAGAGGTATCTGTGCTTTTTTTGTGACCAC1310.5322606858443036No Hit
TAAACAAGGGGTACAATAGAGGACTGTAACTACTATTTAGGTTGTACAGATT1300.5281976271737364No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGTATGCCGTCT1290.5241345685031692No Hit
ACAGCTGGGACAAGTTTTTTTCTTAGTAGTTTTTTTGTTTTGAGTAAATATA1290.5241345685031692No Hit
AGACAAGGACCTGACGTTCCACAAAAACAAGATGAAACTGTGATGCTTGTGA1250.5078823338209004No Hit
TCGAATGGGGGCGCTCTTCCTTTCCAACTTGGACGCTGCAGAATGGCTCCCG1250.5078823338209004No Hit
GGCAACGGGATGTAAGGGCTTTAAATAAAAGAGGTCCATTAATACTTCCTTA1230.499756216479766No Hit
TGCTGTGGGACTTTATAGTAAGAATATAGCTTACTTTTTTTTCCCTAAATAC1180.47944092312692993No Hit
AGGACCGGGGTTCCTGTCCTGTTTCCCCTTCCTCTTTGGGGCTGAGGAGGAG1180.47944092312692993No Hit
TATTGCGGGGTGCTCTGCTGTGTTGTCTTCTCATGGTGAGCTTTTCTGTGCC1170.47537786445636276No Hit
TGTGGTGGGATCTCACTTGAAGTAAATGGACTTAATATTACCCAACTGTGTA1170.47537786445636276No Hit
TTCTCCCGGGGTGCTTTTCTAAGCATTTAAAACATAATTGCCAATTGAAACC1160.4713148057857956No Hit
GCTAGTGGGTAATCCCAACACTTTGGGAGGCCAGGAGTTTTGAGACCAGCCT1110.45099951243295955No Hit
TTAAGCAGGGTTATATTTTCATGTTTTTATAGATACAACATGACAAGAATAC1100.4469364537623924No Hit
TTTATTGGGGGCAGTGACAATGAGGAGGAGGACAAGGAGGCGGCACAGCTGC1090.4428733950918251No Hit
CGCTATGGGGTCACACATCAAAAAAAGATTACCCTTAGTGTATGTGTTTTAA1090.4428733950918251No Hit
TTAGATGGGAGGTTCAGTCATTCCAGTGAGCTCCAAAGAGGGGCCACCTGTT1080.43881033642125794No Hit
GTTTATGGGAATCTTTCCATATTGAGGACAGCCTCTGCATATAAAAATGTTC1040.42255810173898906No Hit
GTTTTAGGGCAAATATGTGTGATCAACCATCTAAAATAAATAAATGCTAACC1040.42255810173898906No Hit
ATACGAGGGACCAGCCCTCCAGCATCTAATAGACTTGAATCTACTCTAAACG990.4022428083861531No Hit
AGATTAGGGATAGTTAGTGCTGTTGGCCATGAGTTCAATGTTAATGAATCAA990.4022428083861531No Hit
GCGCAAGGGGCCCATTAGCACCCAGATAATTCTATCATGTTAGTTTCCCATC980.39817974971558584No Hit
GCCGCAGGGATTTTCCTTCTAGTCTATTTCAGCCTAGAAGAAAAGATAATGA980.39817974971558584No Hit
TATTGTGGGGGATAATCCTGAATTGAGCTGGTCAATAAACTCACAGGGAACA970.3941166910450187No Hit
CATTAGGGAATTTGTGCCATGTGTGAATAAGTGCACAAAATGCTCAAATAAG960.39005363237445145No Hit
GCTTTTGGGGGTTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTCG950.3859905737038843No Hit
GTTTCTGGGCACAGATCCACCTGTCTCTTGAAAATATAAAAAGACAAAACAG940.38192751503331707No Hit
AGTAGCGGGCCGGCCAACAGGCGCACCACGCCCGTCTGAACTGACCTCTGAC910.36973833902161546No Hit
TGTTGAGGGGGTTTTTGGCCTAGGTAACGGGGCAGAGATGTGGTTCGAGATT900.36567528035104824No Hit
CGAATAGGGGATGGATGAGAAAAAACACCACCAAATGACAAGTCACACCCTC890.36161222168048107No Hit
TGCCGAGGGTCACTGAGAACATCAGCTCAAATCAGGAAGGATTCTGCTGTAT880.35754916300991385No Hit
TTAGGCGGTGGTAAAATAATTGAAAAAATACGTTTTCAGGAACAGGTCTTGA880.35754916300991385No Hit
TACTGCGGGAGTTTCTCACAGAATCATTGCAATTATGTTAAAAGAAATCTCT870.3534861043393467No Hit
CGCTGCGGGATGTTTGAGGCTTTTGCTTTAGTTTATAGACTGGTTTATTATA860.34942304566877946No Hit
AAGTCAGGGCTTCTGGGTTTGGCTAGATAGGGTTGTGTCCCTCTATGGAATG850.34535998699821224No Hit
AACTATGGATATGCAATTCTTCTGGTAAATAGCAATAGAATAAAACATATTT840.3412969283276451No Hit
CGTTAAGGGAATCTTGACAATGAGCAGCTGCCATCTTGGGGGATTTCATTCT820.3331708109865107No Hit
TGCCTAGGGGCCCTCGGAGCGGGCGGCGGCGTTGGCGGCTTGTGCAGCAATG820.3331708109865107No Hit
TGTTAAGGGTGGATTCTCTTCTTCACTTTTTCTGACTTTGGGTCCATTTTCT790.320981634974809No Hit
AACTTCGGGAGTGTTAAAAGAAAATAAATTACAATTGATCTGTCATCCATAT790.320981634974809No Hit
GTCACTGGGGGTTTACAGATGAGGAAGTGGATGAGCTGTACAGAGAAGCACC780.31691857630424186No Hit
ATTCGGGGCTCTTTTACCAAGGACCCGCCAACATGGGCCGCGTTCGCACCAA770.31285551763367464No Hit
TGCCGGTGGGCAGATCAATGAATTTCAACTTCTCCCGTAAGGTTTGCTGTAA750.30472940029254025No Hit
TGACGAGGGAGTTTCATGTTGGGTTTAGAAACGCATGACCGAAAATCAGAAG750.30472940029254025No Hit
AGTAATGGGATTCATCGTAAGTCTCCCTTCACCTGTTACAGAGTTTCAGATC720.29254022428083865No Hit
TGGTCGGGTGACTCTTCCAAATTGTATGAATTTATCAATGTATTACACAAAT720.29254022428083865No Hit
CTTGCTGGGAGCACTCAAACCTGGAGCTGAGGAATCTAATTCAGACAGAGAC710.2884771656102714No Hit
GCTTGTGGGACAAAGACAGATGTTTATGGCATTATGCTTGGTTTATTTGTGT700.2844141069397042No Hit
ATAGGTGGGTATAAAAGATGGGGTCTGTAAAATCTTTCTTTCTTAGAAATTT690.280351048269137No Hit
GTGCAGGGGAAGCTCTGAAAATCTGAGGAACATTGTAGGAAAGGTTTGTTAA690.280351048269137No Hit
ACAAATGGGCTCACGACGGGGAGAGCCGGACGGCGCTTCCCGGTGGCGGCGG680.2762879895985698No Hit
CTGGGATTTGTTATGAATGTTATCACAAGTCATTCATCAAGTTATCTTTATA680.2762879895985698No Hit
TGGCACGGGCTCTGATCCTGTAGAAGTTAAGTTCTACCAGATAACTTGCTGC650.2640988135868682No Hit
TTACATGGGTGAGTAACAGAATAAATATTATATATATCAAAAGCCAAAATCT640.260035754916301No Hit
TCACTGGGGAATATTTTGCTGAACTCGTTTTATATTTCTCATGTCTCATATT630.25597269624573377No Hit
AGAACGGGGTGTAAAACAGAAGTTTAAGACCACAAGTTAGAAGAAAGGTCAC630.25597269624573377No Hit
AAATATGGGAGCATTTCTTTTTGGAGGGTTTAGAGATTCTTACTAATATGTT620.25190963757516655No Hit
CAACAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC610.24784657890459938No Hit
AGTTCTGGGACCCTCACTTTGCATGAGGAGCTGAAACTGACCAGTGTCCAGT590.23972046156346496No Hit
GTTTTTGGGTAATAAGACATTTCCTTGTGAGAGGTGGGGGAGAAAAAGGGAA570.23159434422233058No Hit
TAGCGGGATGTCAATCTGTTTTGTTTCTTTTAGTATGGGAAAATTGACATAT570.23159434422233058No Hit
CCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGACT560.22753128555176336No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTGTCTCTT550.2234682268811962No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTGTCTCTTATACA550.2234682268811962No Hit
CAACTAGGGACCGACACCTCAAGGACACTTCTACTTATGAGCAGTTCATCAT550.2234682268811962No Hit
ACTATAGGGCTTCATGTGGAGCCCTCACCACAATCCCTGACTCCGGTCATTT530.21534210954006175No Hit
GAGTTAGGGTCAAATCTGGACCGACAGCAAAGTCCTCTGACTAGAGCCATCG530.21534210954006175No Hit
ATGTGCGGGCGGTAGAATGCTTCATACTTGAGTGATGCTGGATAAGGTATTG520.21127905086949453No Hit
AGAGAGGGCTCCACTTGGATTCTTAGCACTATTGAATTTTGTCTCCATGCCT510.20721599219892736No Hit
CATCGCGGGAGTAGCTTTCCAGCTAAGATAATTGCGTGAACTCTGTTAAAAC500.20315293352836014No Hit
GAAGCTGGGATCCTTCTCCAGTTCCTCCCCCGGCATCGTCCCCGTCCCCACT500.20315293352836014No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGG460.18690069884609134No Hit
GTGCTGGGAATGTATAGTTAATGCTAACATGTATTTATTTTCATATATGTTG450.18283764017552412No Hit
CATACTGGGTCTGTACACAGAACTCTTATCTAGGATGTGGTTCTGTTCATGC450.18283764017552412No Hit
GCCAAAGGGACATGTCAGAGACCACAATGAAAGAATTATTTTAACTTGCATT440.17877458150495693No Hit
GGCCAAGGGAGGAAAGCTGGAGGCGCGGGTGGGGAACATGTCTGAGTCGGAG430.17471152283438973No Hit
TACACTGGGCACCAGGCTGTCTTTAATTTGAGGCCTTTATTGGAATTCTGAA430.17471152283438973No Hit
GTCATAGGGAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGG430.17471152283438973No Hit
TGCATAGGGGGGGGCGGGAGGAGCAAACGCAGGAGGCCATGGCCGCCGCCGC420.17064846416382254No Hit
TCGAGTAGGGATGGCATGTTTAGGACAGTGAAACTATTCTCTATGATACTGT410.16658540549325535No Hit
TTAACAGGGTGTGAGCGTGGACTTGATGCAGTGATGACAAATCATTGCTTAG400.16252234682268812No Hit
GTGTAGGGGGGCCAGACCCTAGGATAACAACACAAACAAGGCCCTTGCTATC400.16252234682268812No Hit
AACCGGGGCAGTTTCATATTTTAGCTATACCTTCTGATACACGTATCTTTCA380.1543962294815537No Hit
AATTCAGGGACGCTGATACATAACTAAATTTGATACTTATATTTTCGTATGA370.15033317081098652No Hit
TTAGATGGGATATCCTGGGGTATAAAATAAATGTTAGTAATTTATTAAATAA370.15033317081098652No Hit
GCCTCCCGTGATGTCCCACCTTTCCTTCAAGGTCTGTCATATGAGTCCTCCC350.1422070534698521No Hit
TGTAGCGGGATTAGAATGAAATTAGGGCCGGGTGCCATGGCTCACACCTGTA340.1381439947992849No Hit
ATGGCCGGGACAGTCTGCTTGTGAAATGCATGCACGTGTATCCACCTCCAGG340.1381439947992849No Hit
TTATGGGGGATGTCTCCTTTGAACACTGATTTTTTTTTTTTAAATTGTCAAA340.1381439947992849No Hit
GCTATAGGGGATTACCGTCTGGTTGTCGTCTGTCATCTCCTCCTGTCTGGCC330.1340809361287177No Hit
CCAAGAGGGGTCTAAGAGCACAAACATAATTCTCCCAAAATTAATTTTGAGA320.1300178774581505No Hit
TTTAGCGGGAATGTGCTACCTTCTATTTAAGAATAATTCCTTCACTCTCTCA300.12189176011701609No Hit
GCTAATGGGAGGCCTCTTGAATGCACACAAAAAATGTGGACTTGCCTGGGGC300.12189176011701609No Hit
TCGACTGGGCGATCTGACGAGGGGGAGAATTTCCCCTCAAGTATGTGGCAGT300.12189176011701609No Hit
GCGATTGGGTCTGTAACATCTTTGTGGTAGTCCTGTTTTCCTAATAACTTTG300.12189176011701609No Hit
GACGCTGGGGGATTCATATGGGAGCGATATGTCATTTAAGTAAAGTTACTGT300.12189176011701609No Hit
ACTAAGGGATTCTTTACCAGTTTTGAAACACTTTGAAATATCCTAAGGTAAC290.1178287014464489No Hit
TCGTATGGGCACGGCTGCGGTGGGCGGGGTCTTCGACGTCTCCAACGCTGAC290.1178287014464489No Hit
GGCCAGAAAGCAGTGCTGAATACTTGAAACTGTGTGCTCTGTTCTACTTAAT290.1178287014464489No Hit
CTTTCTGGGTACCCAGAGGTGGTCACCTACAAGTAAACCCATGTCAGGATAT290.1178287014464489No Hit
TGAATTGGGTAAAATTTATCAAAACATATACACACTCAAACCACATGGTTCC280.11376564277588168No Hit
CTAAACGGGAAAATCACAAATTCTAGTGAATTAAAGTACTTGTGTAGGTGTG270.10970258410531449No Hit
GTGGCTGGGATGAACTATAGAAAAAAATCTATATATAATGTACATAAATGTT270.10970258410531449No Hit
GAATCCCCACATCGTCCTCAACATTGACCTGGCCCCCACCATCCTGGACATT260.10563952543474726No Hit
GGTAACGGGATATTGGGTTATTCACCATAAAGGATCCAATAGCAATTCTAAA260.10563952543474726No Hit
ACGCCTGGGTGGAAATTGACGAAGTATAATTATTCTAAGATGGAATAATTAA260.10563952543474726No Hit
GGTTCCGGGTTGCCCAGCCGTGGTGGCCCCCAGGCCTGGTCCCCAAGAGCTT250.10157646676418007No Hit
CATATTGGGCGACGCTCTTCCGATCTATCTAAGGGATCAGTGAAAAGGATCA250.10157646676418007No Hit
GTAGCAGGGCTGTTACTTAAATAGCATCTGCTCTTCTCTTACGCTCTCTGTC250.10157646676418007No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)