FastQCFastQC Report
Sat 31 Dec 2022
SRR5844300.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844300.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24081
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTACGGGCTCCAAGACTTCATCAATATTAAACTCAATTCAGCTTCTATCT2320.9634151405672522No Hit
CCGACTGGGCATACAACAATTGAAAGTGTTTGCTCATGAAATCAGTTATAAA2160.8969727170798555No Hit
AAGGCTGGGCTCTGCCGGTGCAGAATATGAACAATGCCATGTTCTTGCAGAA2130.8845147626759685No Hit
TTCACTGGGAGATTATTTCTGTAGTTATATTTCTCCACTCTTTCTCGAATTT2110.8762094597400439No Hit
GGCTGCGGGTGGGGTTCAAGGTGATAAAGTTTTTCTAGTAAGGCTCCCCTCG1970.8180723391885719No Hit
ACTTTTGGGAAGAAAATAGTGGTGTGATTTGGGGATATTTTTTTCTTTTCTT1860.7723931730409866No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGTATGC1800.7474772642332128No Hit
TTATCTGGGATACTCTTCAATCAGCCACATAGCCCTCGTAGTAACAGCCATT1740.7225613554254391No Hit
TAGCTTGGGAGTGTGTAAGCTAAACAAAAACTAAAACTAAGAATTCTCAAAA1730.7184087039574768No Hit
TTAAAGGGGTGCTTCTTTCTTTGGGAATATTTGAATCTTGTCTCAATGCTCA1700.70595074955359No Hit
ATTCATGGGGCAGGTGCTCCTCGATGTCCTTGCGGTCGTAGGTGATGCCACT1660.6893401436817408No Hit
CTTATGGGGTACTTATTTTATTGATTCAGTTTTGCTAATTTTGCTAGTAGGA1640.6810348407458162No Hit
CATAGGGGGGCAAATAGGGAGATTATCCTGAATTATCTCTTAGAATCCAAGG1630.6768821892778539No Hit
CGTTGAGGGGACTGAAGAAACCCGCCGGCTGCTGGGGCAGGGCGGCGAAAGG1590.6602715834060047No Hit
ATTAGTGGGGTGTTCCTTGGCTTAAAGCCCCTTCCTCCATCTGCAAAGCCAG1580.6561189319380424No Hit
TCAATTGGGATCAGCTCAAGTTGTACTCAGAGAAGCAGAGGAACTGGGAGGA1580.6561189319380424No Hit
TATTAGGGGACAGTTTCTTGTTTCGAGTGCTGACTGACCCGTTTCTGAAATG1560.6478136290021179No Hit
CTTCAAGAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC1530.635355674598231No Hit
GACAGGGAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGA1520.6312030231302687No Hit
TCACAGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1480.6145924172584195No Hit
TCGAATGGGGGCGCTCTTCCTTTCCAACTTGGACGCTGCAGAATGGCTCCCG1480.6145924172584195No Hit
TGATATGGGGGGGACGGACCGGAAGTGCCCGAGGGCGGCCGCAGAACGGTCA1470.6104397657904572No Hit
AGCCTAGGGTCTGTCGTTGGGCTTTGGTATGATGTTAGCCATGGGTTTGTCA1450.6021344628545326No Hit
ATACCTGGGGTTATGACTTTGATGTGCATACAATGAAGACACTAAAAAACAT1430.593829159918608No Hit
GAAACAGGGCATCATGAACTTGGACCAAAATGAATTCGCTGGCTTCTCTTAT1420.5896765084506458No Hit
AGTCAAGGGTGGGAATGATGATTTCACAGACTAGAGTCTCCGATGCTGGTCA1400.5813712055147212No Hit
ACAGCTGGGACAAGTTTTTTTCTTAGTAGTTTTTTTGTTTTGAGTAAATATA1380.5730659025787965No Hit
TATTGCGGGGTGCTCTGCTGTGTTGTCTTCTCATGGTGAGCTTTTCTGTGCC1330.5523026452389851No Hit
GCCGCAGGGATTTTCCTTCTAGTCTATTTCAGCCTAGAAGAAAAGATAATGA1300.5398446908350982No Hit
AGGACCGGGGTTCCTGTCCTGTTTCCCCTTCCTCTTTGGGGCTGAGGAGGAG1290.5356920393671358No Hit
AAGCCAGGGACCTACCCAGTATTGTGTCAAGCTTGAACAATAAAATAGAGTT1260.5232340849632491No Hit
TAAACAAGGGGTACAATAGAGGACTGTAACTACTATTTAGGTTGTACAGATT1260.5232340849632491No Hit
TGCTGTGGGACTTTATAGTAAGAATATAGCTTACTTTTTTTTCCCTAAATAC1240.5149287820273244No Hit
TTAAGCAGGGTTATATTTTCATGTTTTTATAGATACAACATGACAAGAATAC1220.5066234790913998No Hit
ATACGAGGGACCAGCCCTCCAGCATCTAATAGACTTGAATCTACTCTAAACG1200.4983181761554753No Hit
TTTATTGGGGGCAGTGACAATGAGGAGGAGGACAAGGAGGCGGCACAGCTGC1200.4983181761554753No Hit
GCTAGTGGGTAATCCCAACACTTTGGGAGGCCAGGAGTTTTGAGACCAGCCT1190.49416552468751296No Hit
CATTAGGGAATTTGTGCCATGTGTGAATAAGTGCACAAAATGCTCAAATAAG1180.49001287321955067No Hit
AGATTAGGGATAGTTAGTGCTGTTGGCCATGAGTTCAATGTTAATGAATCAA1170.4858602217515884No Hit
GTCAATGGGAATGCTTTTTTGTTTTTGCTTTTTCTAACAAAACAATCTTTTT1140.47340226734770147No Hit
AGACAAGGACCTGACGTTCCACAAAAACAAGATGAAACTGTGATGCTTGTGA1120.4650969644117769No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGTATGCCGTCT1100.4567916614758524No Hit
TGTGGTGGGATCTCACTTGAAGTAAATGGACTTAATATTACCCAACTGTGTA1080.44848635853992774No Hit
GCGCAAGGGGCCCATTAGCACCCAGATAATTCTATCATGTTAGTTTCCCATC1070.44433370707196546No Hit
TGCCTAGGGGCCCTCGGAGCGGGCGGCGGCGTTGGCGGCTTGTGCAGCAATG1070.44433370707196546No Hit
GTTTATGGGAATCTTTCCATATTGAGGACAGCCTCTGCATATAAAAATGTTC1060.44018105560400317No Hit
CGCTATGGGGTCACACATCAAAAAAAGATTACCCTTAGTGTATGTGTTTTAA1060.44018105560400317No Hit
GTTTTAGGGCAAATATGTGTGATCAACCATCTAAAATAAATAAATGCTAACC1020.42357044973215396No Hit
GGCAACGGGATGTAAGGGCTTTAAATAAAAGAGGTCCATTAATACTTCCTTA1020.42357044973215396No Hit
TGTTGAGGGGGTTTTTGGCCTAGGTAACGGGGCAGAGATGTGGTTCGAGATT990.41111249532826716No Hit
TTAGGCGGTGGTAAAATAATTGAAAAAATACGTTTTCAGGAACAGGTCTTGA980.40695984386030476No Hit
TTAGATGGGAGGTTCAGTCATTCCAGTGAGCTCCAAAGAGGGGCCACCTGTT960.39865454092438024No Hit
TGCCGAGGGTCACTGAGAACATCAGCTCAAATCAGGAAGGATTCTGCTGTAT950.39450188945641795No Hit
TGTGCTGGCATGAATATTCTAAAAGAGGTATCTGTGCTTTTTTTGTGACCAC940.3903492379884556No Hit
ACAAATGGGCTCACGACGGGGAGAGCCGGACGGCGCTTCCCGGTGGCGGCGG920.38204393505253104No Hit
TTCTCCCGGGGTGCTTTTCTAAGCATTTAAAACATAATTGCCAATTGAAACC890.3695859806486442No Hit
CGTTAAGGGAATCTTGACAATGAGCAGCTGCCATCTTGGGGGATTTCATTCT840.3488227233088327No Hit
AGTAGCGGGCCGGCCAACAGGCGCACCACGCCCGTCTGAACTGACCTCTGAC840.3488227233088327No Hit
ATAGGTGGGTATAAAAGATGGGGTCTGTAAAATCTTTCTTTCTTAGAAATTT820.3405174203729081No Hit
CGCTGCGGGATGTTTGAGGCTTTTGCTTTAGTTTATAGACTGGTTTATTATA810.3363647689049458No Hit
GCTTTTGGGGGTTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTCG780.32390681450105896No Hit
GTTTCTGGGCACAGATCCACCTGTCTCTTGAAAATATAAAAAGACAAAACAG780.32390681450105896No Hit
TACTGCGGGAGTTTCTCACAGAATCATTGCAATTATGTTAAAAGAAATCTCT780.32390681450105896No Hit
AAGTCAGGGCTTCTGGGTTTGGCTAGATAGGGTTGTGTCCCTCTATGGAATG770.3197541630330966No Hit
CTTGCTGGGAGCACTCAAACCTGGAGCTGAGGAATCTAATTCAGACAGAGAC770.3197541630330966No Hit
TATTGTGGGGGATAATCCTGAATTGAGCTGGTCAATAAACTCACAGGGAACA760.31560151156513433No Hit
TTACATGGGTGAGTAACAGAATAAATATTATATATATCAAAAGCCAAAATCT750.31144886009717204No Hit
CGAATAGGGGATGGATGAGAAAAAACACCACCAAATGACAAGTCACACCCTC740.30729620862920975No Hit
ATTCGGGGCTCTTTTACCAAGGACCCGCCAACATGGGCCGCGTTCGCACCAA730.30314355716124747No Hit
TGACGAGGGAGTTTCATGTTGGGTTTAGAAACGCATGACCGAAAATCAGAAG730.30314355716124747No Hit
TGGCACGGGCTCTGATCCTGTAGAAGTTAAGTTCTACCAGATAACTTGCTGC730.30314355716124747No Hit
TGTTAAGGGTGGATTCTCTTCTTCACTTTTTCTGACTTTGGGTCCATTTTCT720.2989909056932851No Hit
CAACAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC720.2989909056932851No Hit
CCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGACT720.2989909056932851No Hit
GTCACTGGGGGTTTACAGATGAGGAAGTGGATGAGCTGTACAGAGAAGCACC720.2989909056932851No Hit
GTGCAGGGGAAGCTCTGAAAATCTGAGGAACATTGTAGGAAAGGTTTGTTAA710.2948382542253229No Hit
GCTTGTGGGACAAAGACAGATGTTTATGGCATTATGCTTGGTTTATTTGTGT690.28653295128939826No Hit
AGAACGGGGTGTAAAACAGAAGTTTAAGACCACAAGTTAGAAGAAAGGTCAC660.2740749968855114No Hit
AGTAATGGGATTCATCGTAAGTCTCCCTTCACCTGTTACAGAGTTTCAGATC650.2699223454175491No Hit
GTAGCAGGGCTGTTACTTAAATAGCATCTGCTCTTCTCTTACGCTCTCTGTC620.2574643910136622No Hit
TGGTCGGGTGACTCTTCCAAATTGTATGAATTTATCAATGTATTACACAAAT620.2574643910136622No Hit
TGCCGGTGGGCAGATCAATGAATTTCAACTTCTCCCGTAAGGTTTGCTGTAA610.2533117395456999No Hit
TCACTGGGGAATATTTTGCTGAACTCGTTTTATATTTCTCATGTCTCATATT610.2533117395456999No Hit
CAACTAGGGACCGACACCTCAAGGACACTTCTACTTATGAGCAGTTCATCAT610.2533117395456999No Hit
AAATATGGGAGCATTTCTTTTTGGAGGGTTTAGAGATTCTTACTAATATGTT580.24085378514181305No Hit
AACTATGGATATGCAATTCTTCTGGTAAATAGCAATAGAATAAAACATATTT570.23670113367385073No Hit
CTGGGATTTGTTATGAATGTTATCACAAGTCATTCATCAAGTTATCTTTATA570.23670113367385073No Hit
AACTTCGGGAGTGTTAAAAGAAAATAAATTACAATTGATCTGTCATCCATAT560.23254848220588845No Hit
GTTTTTGGGTAATAAGACATTTCCTTGTGAGAGGTGGGGGAGAAAAAGGGAA560.23254848220588845No Hit
GAGTTAGGGTCAAATCTGGACCGACAGCAAAGTCCTCTGACTAGAGCCATCG540.22424317926996387No Hit
GGCCAAGGGAGGAAAGCTGGAGGCGCGGGTGGGGAACATGTCTGAGTCGGAG530.22009052780200158No Hit
GCCAAAGGGACATGTCAGAGACCACAATGAAAGAATTATTTTAACTTGCATT530.22009052780200158No Hit
GAAGCTGGGATCCTTCTCCAGTTCCTCCCCCGGCATCGTCCCCGTCCCCACT510.21178522486607698No Hit
CATCGCGGGAGTAGCTTTCCAGCTAAGATAATTGCGTGAACTCTGTTAAAAC500.2076325733981147No Hit
ACTATAGGGCTTCATGTGGAGCCCTCACCACAATCCCTGACTCCGGTCATTT490.20347992193015238No Hit
TGCATAGGGGGGGGCGGGAGGAGCAAACGCAGGAGGCCATGGCCGCCGCCGC490.20347992193015238No Hit
TGTAGCGGGATTAGAATGAAATTAGGGCCGGGTGCCATGGCTCACACCTGTA480.19932727046219012No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTGTCTCTTATACA480.19932727046219012No Hit
TAGCGGGATGTCAATCTGTTTTGTTTCTTTTAGTATGGGAAAATTGACATAT480.19932727046219012No Hit
GTGTAGGGGGGCCAGACCCTAGGATAACAACACAAACAAGGCCCTTGCTATC460.19102196752626552No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTGTCTCTT460.19102196752626552No Hit
GTGCTGGGAATGTATAGTTAATGCTAACATGTATTTATTTTCATATATGTTG450.1868693160583032No Hit
CATATTGGGCGACGCTCTTCCGATCTATCTAAGGGATCAGTGAAAAGGATCA450.1868693160583032No Hit
AGTTCTGGGACCCTCACTTTGCATGAGGAGCTGAAACTGACCAGTGTCCAGT430.17856401312237863No Hit
GTCATAGGGAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGG430.17856401312237863No Hit
TCGAGTAGGGATGGCATGTTTAGGACAGTGAAACTATTCTCTATGATACTGT430.17856401312237863No Hit
AGAGAGGGCTCCACTTGGATTCTTAGCACTATTGAATTTTGTCTCCATGCCT420.17441136165441634No Hit
TTATGGGGGATGTCTCCTTTGAACACTGATTTTTTTTTTTTAAATTGTCAAA400.16610605871849177No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGG390.16195340725052948No Hit
CCAAGAGGGGTCTAAGAGCACAAACATAATTCTCCCAAAATTAATTTTGAGA390.16195340725052948No Hit
ATGTGCGGGCGGTAGAATGCTTCATACTTGAGTGATGCTGGATAAGGTATTG390.16195340725052948No Hit
TGAATTGGGTAAAATTTATCAAAACATATACACACTCAAACCACATGGTTCC380.15780075578256716No Hit
TCGTATGGGCACGGCTGCGGTGGGCGGGGTCTTCGACGTCTCCAACGCTGAC370.15364810431460488No Hit
TTTAGCGGGAATGTGCTACCTTCTATTTAAGAATAATTCCTTCACTCTCTCA370.15364810431460488No Hit
CATACTGGGTCTGTACACAGAACTCTTATCTAGGATGTGGTTCTGTTCATGC370.15364810431460488No Hit
GAATCCCCACATCGTCCTCAACATTGACCTGGCCCCCACCATCCTGGACATT360.14949545284664256No Hit
CTAAACGGGAAAATCACAAATTCTAGTGAATTAAAGTACTTGTGTAGGTGTG360.14949545284664256No Hit
GGTTCCGGGTTGCCCAGCCGTGGTGGCCCCCAGGCCTGGTCCCCAAGAGCTT340.141190149910718No Hit
TAGCCCGGGCTACGATGGGGATTGGTACTATATGAAAGTGAGTCAACTAATT340.141190149910718No Hit
CTTTCTGGGTACCCAGAGGTGGTCACCTACAAGTAAACCCATGTCAGGATAT330.1370374984427557No Hit
AATTCAGGGACGCTGATACATAACTAAATTTGATACTTATATTTTCGTATGA320.1328848469747934No Hit
GGCTTAGGGAGGGTTAATAATTGTTTCTCCTTTTTAATTTGCTTTATTTCCT310.1287321955068311No Hit
ATGGCCGGGACAGTCTGCTTGTGAAATGCATGCACGTGTATCCACCTCCAGG300.12457954403886883No Hit
GCTATAGGGGATTACCGTCTGGTTGTCGTCTGTCATCTCCTCCTGTCTGGCC300.12457954403886883No Hit
GACGCTGGGGGATTCATATGGGAGCGATATGTCATTTAAGTAAAGTTACTGT300.12457954403886883No Hit
TTAGATGGGATATCCTGGGGTATAAAATAAATGTTAGTAATTTATTAAATAA290.12042689257090652No Hit
TTGTATGGGTGCGACTTGATACTTGGAAATGTGTGTGACTTTTACAAAGAGC290.12042689257090652No Hit
TTAACAGGGTGTGAGCGTGGACTTGATGCAGTGATGACAAATCATTGCTTAG280.11627424110294422No Hit
GGCCAGAAAGCAGTGCTGAATACTTGAAACTGTGTGCTCTGTTCTACTTAAT280.11627424110294422No Hit
AACCGGGGCAGTTTCATATTTTAGCTATACCTTCTGATACACGTATCTTTCA270.11212158963498194No Hit
GCCTCCCGTGATGTCCCACCTTTCCTTCAAGGTCTGTCATATGAGTCCTCCC270.11212158963498194No Hit
TACTCTGGGTACTCCCGGGGTCTGCAGACCCTCTGAATAGCCATCTCAAAAT260.10796893816701965No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGA250.10381628669905735No Hit
GTGGCTGGGATGAACTATAGAAAAAAATCTATATATAATGTACATAAATGTT250.10381628669905735No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)