FastQCFastQC Report
Sat 31 Dec 2022
SRR5844299.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844299.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24556
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGGGCTCTGCCGGTGCAGAATATGAACAATGCCATGTTCTTGCAGAA2350.9569962534614759No Hit
CATAGGGGGGCAAATAGGGAGATTATCCTGAATTATCTCTTAGAATCCAAGG2280.9284899820817722No Hit
GGCTGCGGGTGGGGTTCAAGGTGATAAAGTTTTTCTAGTAAGGCTCCCCTCG2090.8511158169082912No Hit
TTATCTGGGATACTCTTCAATCAGCCACATAGCCCTCGTAGTAACAGCCATT2070.8429711679426617No Hit
AGTTACGGGCTCCAAGACTTCATCAATATTAAACTCAATTCAGCTTCTATCT2060.8388988434598468No Hit
TTCACTGGGAGATTATTTCTGTAGTTATATTTCTCCACTCTTTCTCGAATTT1990.8103925720801434No Hit
ATTAGTGGGGTGTTCCTTGGCTTAAAGCCCCTTCCTCCATCTGCAAAGCCAG1950.7941032741488842No Hit
ACTTTTGGGAAGAAAATAGTGGTGTGATTTGGGGATATTTTTTTCTTTTCTT1920.7818863007004397No Hit
TTAAAGGGGTGCTTCTTTCTTTGGGAATATTTGAATCTTGTCTCAATGCTCA1870.7615246782863658No Hit
TAGCTTGGGAGTGTGTAAGCTAAACAAAAACTAAAACTAAGAATTCTCAAAA1820.7411630558722919No Hit
TCAATTGGGATCAGCTCAAGTTGTACTCAGAGAAGCAGAGGAACTGGGAGGA1820.7411630558722919No Hit
CCGACTGGGCATACAACAATTGAAAGTGTTTGCTCATGAAATCAGTTATAAA1820.7411630558722919No Hit
CTTATGGGGTACTTATTTTATTGATTCAGTTTTGCTAATTTTGCTAGTAGGA1730.7045121355269588No Hit
GACAGGGAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTTAAGA1710.6963674865613292No Hit
CTTCAAGAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC1650.6719335396644405No Hit
TCACAGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1580.643427268284737No Hit
ATTCATGGGGCAGGTGCTCCTCGATGTCCTTGCGGTCGTAGGTGATGCCACT1560.6352826193191073No Hit
GAAACAGGGCATCATGAACTTGGACCAAAATGAATTCGCTGGCTTCTCTTAT1530.6230656458706629No Hit
CGTTGAGGGGACTGAAGAAACCCGCCGGCTGCTGGGGCAGGGCGGCGAAAGG1520.6189933213878481No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGTATGC1500.6108486724222186No Hit
AGTCAAGGGTGGGAATGATGATTTCACAGACTAGAGTCTCCGATGCTGGTCA1460.5945593744909595No Hit
TGCTGTGGGACTTTATAGTAAGAATATAGCTTACTTTTTTTTCCCTAAATAC1450.5904870500081446No Hit
AAGCCAGGGACCTACCCAGTATTGTGTCAAGCTTGAACAATAAAATAGAGTT1420.5782700765597002No Hit
TAAACAAGGGGTACAATAGAGGACTGTAACTACTATTTAGGTTGTACAGATT1410.5741977520768855No Hit
TATTAGGGGACAGTTTCTTGTTTCGAGTGCTGACTGACCCGTTTCTGAAATG1390.5660531031112559No Hit
TATTGCGGGGTGCTCTGCTGTGTTGTCTTCTCATGGTGAGCTTTTCTGTGCC1380.5619807786284411No Hit
ACAGCTGGGACAAGTTTTTTTCTTAGTAGTTTTTTTGTTTTGAGTAAATATA1350.5497638051799967No Hit
AGCCTAGGGTCTGTCGTTGGGCTTTGGTATGATGTTAGCCATGGGTTTGTCA1350.5497638051799967No Hit
TTAAGCAGGGTTATATTTTCATGTTTTTATAGATACAACATGACAAGAATAC1340.545691480697182No Hit
AGACAAGGACCTGACGTTCCACAAAAACAAGATGAAACTGTGATGCTTGTGA1340.545691480697182No Hit
TGATATGGGGGGGACGGACCGGAAGTGCCCGAGGGCGGCCGCAGAACGGTCA1310.5334745072487376No Hit
ATACGAGGGACCAGCCCTCCAGCATCTAATAGACTTGAATCTACTCTAAACG1290.5253298582831081No Hit
GTCAATGGGAATGCTTTTTTGTTTTTGCTTTTTCTAACAAAACAATCTTTTT1260.5131128848346637No Hit
TTAGATGGGAGGTTCAGTCATTCCAGTGAGCTCCAAAGAGGGGCCACCTGTT1240.504968235869034No Hit
TTTATTGGGGGCAGTGACAATGAGGAGGAGGACAAGGAGGCGGCACAGCTGC1230.5008959113862193No Hit
GCTAGTGGGTAATCCCAACACTTTGGGAGGCCAGGAGTTTTGAGACCAGCCT1230.5008959113862193No Hit
GTTTATGGGAATCTTTCCATATTGAGGACAGCCTCTGCATATAAAAATGTTC1220.4968235869034045No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGTATGCCGTCT1220.4968235869034045No Hit
TCGAATGGGGGCGCTCTTCCTTTCCAACTTGGACGCTGCAGAATGGCTCCCG1150.4683173155237009No Hit
CGCTATGGGGTCACACATCAAAAAAAGATTACCCTTAGTGTATGTGTTTTAA1150.4683173155237009No Hit
TACTGCGGGAGTTTCTCACAGAATCATTGCAATTATGTTAAAAGAAATCTCT1140.4642449910408861No Hit
GTTTTAGGGCAAATATGTGTGATCAACCATCTAAAATAAATAAATGCTAACC1140.4642449910408861No Hit
GCCGCAGGGATTTTCCTTCTAGTCTATTTCAGCCTAGAAGAAAAGATAATGA1130.4601726665580713No Hit
ATACCTGGGGTTATGACTTTGATGTGCATACAATGAAGACACTAAAAAACAT1120.45610034207525657No Hit
GGCAACGGGATGTAAGGGCTTTAAATAAAAGAGGTCCATTAATACTTCCTTA1120.45610034207525657No Hit
AGGACCGGGGTTCCTGTCCTGTTTCCCCTTCCTCTTTGGGGCTGAGGAGGAG1090.4438833686268122No Hit
TGTGGTGGGATCTCACTTGAAGTAAATGGACTTAATATTACCCAACTGTGTA1080.4398110441439974No Hit
TTCTCCCGGGGTGCTTTTCTAAGCATTTAAAACATAATTGCCAATTGAAACC1070.43573871966118255No Hit
TGCCTAGGGGCCCTCGGAGCGGGCGGCGGCGTTGGCGGCTTGTGCAGCAATG1070.43573871966118255No Hit
CGCTGCGGGATGTTTGAGGCTTTTGCTTTAGTTTATAGACTGGTTTATTATA1050.42759407069555305No Hit
GCGCAAGGGGCCCATTAGCACCCAGATAATTCTATCATGTTAGTTTCCCATC1030.4194494217299234No Hit
TGTTGAGGGGGTTTTTGGCCTAGGTAACGGGGCAGAGATGTGGTTCGAGATT1000.40723244828147903No Hit
AGATTAGGGATAGTTAGTGCTGTTGGCCATGAGTTCAATGTTAATGAATCAA950.3868708258674051No Hit
CATTAGGGAATTTGTGCCATGTGTGAATAAGTGCACAAAATGCTCAAATAAG940.3827985013845903No Hit
ACAAATGGGCTCACGACGGGGAGAGCCGGACGGCGCTTCCCGGTGGCGGCGG930.3787261769017755No Hit
ATAGGTGGGTATAAAAGATGGGGTCTGTAAAATCTTTCTTTCTTAGAAATTT930.3787261769017755No Hit
TGCCGAGGGTCACTGAGAACATCAGCTCAAATCAGGAAGGATTCTGCTGTAT920.3746538524189607No Hit
TGTGCTGGCATGAATATTCTAAAAGAGGTATCTGTGCTTTTTTTGTGACCAC890.3624368789705164No Hit
GCTTTTGGGGGTTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTCG870.35429223000488674No Hit
AACTATGGATATGCAATTCTTCTGGTAAATAGCAATAGAATAAAACATATTT870.35429223000488674No Hit
TATTGTGGGGGATAATCCTGAATTGAGCTGGTCAATAAACTCACAGGGAACA870.35429223000488674No Hit
TTAGGCGGTGGTAAAATAATTGAAAAAATACGTTTTCAGGAACAGGTCTTGA860.350219905522072No Hit
GTTTCTGGGCACAGATCCACCTGTCTCTTGAAAATATAAAAAGACAAAACAG850.3461475810392572No Hit
ATTCGGGGCTCTTTTACCAAGGACCCGCCAACATGGGCCGCGTTCGCACCAA840.34207525655644244No Hit
TGTTAAGGGTGGATTCTCTTCTTCACTTTTTCTGACTTTGGGTCCATTTTCT820.33393060759081283No Hit
AGTAGCGGGCCGGCCAACAGGCGCACCACGCCCGTCTGAACTGACCTCTGAC810.32985828310799803No Hit
CAACAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC800.3257859586251832No Hit
AACTTCGGGAGTGTTAAAAGAAAATAAATTACAATTGATCTGTCATCCATAT790.3217136341423685No Hit
TGCCGGTGGGCAGATCAATGAATTTCAACTTCTCCCGTAAGGTTTGCTGTAA770.31356898517673887No Hit
TGACGAGGGAGTTTCATGTTGGGTTTAGAAACGCATGACCGAAAATCAGAAG760.30949666069392406No Hit
AAGTCAGGGCTTCTGGGTTTGGCTAGATAGGGTTGTGTCCCTCTATGGAATG760.30949666069392406No Hit
CGTTAAGGGAATCTTGACAATGAGCAGCTGCCATCTTGGGGGATTTCATTCT760.30949666069392406No Hit
CAACTAGGGACCGACACCTCAAGGACACTTCTACTTATGAGCAGTTCATCAT760.30949666069392406No Hit
GCTTGTGGGACAAAGACAGATGTTTATGGCATTATGCTTGGTTTATTTGTGT730.29727968724547976No Hit
TTACATGGGTGAGTAACAGAATAAATATTATATATATCAAAAGCCAAAATCT710.2891350382798501No Hit
GTGCAGGGGAAGCTCTGAAAATCTGAGGAACATTGTAGGAAAGGTTTGTTAA710.2891350382798501No Hit
GTCACTGGGGGTTTACAGATGAGGAAGTGGATGAGCTGTACAGAGAAGCACC710.2891350382798501No Hit
CGAATAGGGGATGGATGAGAAAAAACACCACCAAATGACAAGTCACACCCTC700.28506271379703535No Hit
TAGCGGGATGTCAATCTGTTTTGTTTCTTTTAGTATGGGAAAATTGACATAT700.28506271379703535No Hit
CTGGGATTTGTTATGAATGTTATCACAAGTCATTCATCAAGTTATCTTTATA690.28099038931422055No Hit
AGTAATGGGATTCATCGTAAGTCTCCCTTCACCTGTTACAGAGTTTCAGATC660.2687734158657762No Hit
TGGCACGGGCTCTGATCCTGTAGAAGTTAAGTTCTACCAGATAACTTGCTGC660.2687734158657762No Hit
CTTGCTGGGAGCACTCAAACCTGGAGCTGAGGAATCTAATTCAGACAGAGAC650.2647010913829614No Hit
TGGTCGGGTGACTCTTCCAAATTGTATGAATTTATCAATGTATTACACAAAT630.25655644241733183No Hit
AAATATGGGAGCATTTCTTTTTGGAGGGTTTAGAGATTCTTACTAATATGTT620.252484117934517No Hit
CATCGCGGGAGTAGCTTTCCAGCTAAGATAATTGCGTGAACTCTGTTAAAAC610.24841179345170225No Hit
GAGTTAGGGTCAAATCTGGACCGACAGCAAAGTCCTCTGACTAGAGCCATCG600.24433946896888745No Hit
AGAACGGGGTGTAAAACAGAAGTTTAAGACCACAAGTTAGAAGAAAGGTCAC580.23619482000325787No Hit
AGAGAGGGCTCCACTTGGATTCTTAGCACTATTGAATTTTGTCTCCATGCCT570.23212249552044306No Hit
AGTTCTGGGACCCTCACTTTGCATGAGGAGCTGAAACTGACCAGTGTCCAGT530.2158331975891839No Hit
TCGAGTAGGGATGGCATGTTTAGGACAGTGAAACTATTCTCTATGATACTGT530.2158331975891839No Hit
GAAGCTGGGATCCTTCTCCAGTTCCTCCCCCGGCATCGTCCCCGTCCCCACT520.21176087310636912No Hit
GCCAAAGGGACATGTCAGAGACCACAATGAAAGAATTATTTTAACTTGCATT520.21176087310636912No Hit
TTAGATGGGATATCCTGGGGTATAAAATAAATGTTAGTAATTTATTAAATAA510.20768854862355432No Hit
ACTATAGGGCTTCATGTGGAGCCCTCACCACAATCCCTGACTCCGGTCATTT500.20361622414073952No Hit
ATGTGCGGGCGGTAGAATGCTTCATACTTGAGTGATGCTGGATAAGGTATTG500.20361622414073952No Hit
CCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGACT480.19547157517510994No Hit
TGCATAGGGGGGGGCGGGAGGAGCAAACGCAGGAGGCCATGGCCGCCGCCGC460.18732692620948035No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGG450.18325460172666558No Hit
AATTCAGGGACGCTGATACATAACTAAATTTGATACTTATATTTTCGTATGA450.18325460172666558No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTGTCTCTTATACA450.18325460172666558No Hit
GGCCAAGGGAGGAAAGCTGGAGGCGCGGGTGGGGAACATGTCTGAGTCGGAG440.1791822772438508No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTGTCTCTT440.1791822772438508No Hit
CCAAGAGGGGTCTAAGAGCACAAACATAATTCTCCCAAAATTAATTTTGAGA430.175109952761036No Hit
GCTATAGGGGATTACCGTCTGGTTGTCGTCTGTCATCTCCTCCTGTCTGGCC430.175109952761036No Hit
GTAGCAGGGCTGTTACTTAAATAGCATCTGCTCTTCTCTTACGCTCTCTGTC420.17103762827822122No Hit
TGTAGCGGGATTAGAATGAAATTAGGGCCGGGTGCCATGGCTCACACCTGTA410.16696530379540642No Hit
GCCTCCCGTGATGTCCCACCTTTCCTTCAAGGTCTGTCATATGAGTCCTCCC410.16696530379540642No Hit
GTGCTGGGAATGTATAGTTAATGCTAACATGTATTTATTTTCATATATGTTG400.1628929793125916No Hit
GTTTTTGGGTAATAAGACATTTCCTTGTGAGAGGTGGGGGAGAAAAAGGGAA400.1628929793125916No Hit
CATACTGGGTCTGTACACAGAACTCTTATCTAGGATGTGGTTCTGTTCATGC390.15882065482977684No Hit
GTGGCTGGGATGAACTATAGAAAAAAATCTATATATAATGTACATAAATGTT390.15882065482977684No Hit
TCACTGGGGAATATTTTGCTGAACTCGTTTTATATTTCTCATGTCTCATATT380.15474833034696203No Hit
TCGTATGGGCACGGCTGCGGTGGGCGGGGTCTTCGACGTCTCCAACGCTGAC370.15067600586414726No Hit
GGTTCCGGGTTGCCCAGCCGTGGTGGCCCCCAGGCCTGGTCCCCAAGAGCTT360.14660368138133248No Hit
GCGATTGGGTCTGTAACATCTTTGTGGTAGTCCTGTTTTCCTAATAACTTTG360.14660368138133248No Hit
TGAATTGGGTAAAATTTATCAAAACATATACACACTCAAACCACATGGTTCC360.14660368138133248No Hit
TTTAGCGGGAATGTGCTACCTTCTATTTAAGAATAATTCCTTCACTCTCTCA350.14253135689851767No Hit
TTATGGGGGATGTCTCCTTTGAACACTGATTTTTTTTTTTTAAATTGTCAAA350.14253135689851767No Hit
TACACTGGGCACCAGGCTGTCTTTAATTTGAGGCCTTTATTGGAATTCTGAA350.14253135689851767No Hit
GTCATAGGGAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGG350.14253135689851767No Hit
CATATTGGGCGACGCTCTTCCGATCTATCTAAGGGATCAGTGAAAAGGATCA330.1343867079328881No Hit
TTAACAGGGTGTGAGCGTGGACTTGATGCAGTGATGACAAATCATTGCTTAG320.1303143834500733No Hit
GTGTAGGGGGGCCAGACCCTAGGATAACAACACAAACAAGGCCCTTGCTATC320.1303143834500733No Hit
TTTTTAGGGACTTTTAATTGAGTGTGCAATCGTTTTCCATGGAGTTTGGTCC320.1303143834500733No Hit
ATGGCCGGGACAGTCTGCTTGTGAAATGCATGCACGTGTATCCACCTCCAGG320.1303143834500733No Hit
TAGCCCGGGCTACGATGGGGATTGGTACTATATGAAAGTGAGTCAACTAATT290.11809741000162893No Hit
GCTAATGGGAGGCCTCTTGAATGCACACAAAAAATGTGGACTTGCCTGGGGC290.11809741000162893No Hit
GAATCCCCACATCGTCCTCAACATTGACCTGGCCCCCACCATCCTGGACATT290.11809741000162893No Hit
AACCGGGGCAGTTTCATATTTTAGCTATACCTTCTGATACACGTATCTTTCA290.11809741000162893No Hit
GGCAAGAGGGCTTGGGCCCCAGCCCAAGGGTGGAGAGGGGGCCAAGGGCTCC280.11402508551881414No Hit
GAATAGGGGACACACACACACACACACACAAACACGAATTAAGAAACTGGAG260.10588043655318456No Hit
GGCCAGAAAGCAGTGCTGAATACTTGAAACTGTGTGCTCTGTTCTACTTAAT260.10588043655318456No Hit
CTTTCTGGGTACCCAGAGGTGGTCACCTACAAGTAAACCCATGTCAGGATAT260.10588043655318456No Hit
GACGCTGGGGGATTCATATGGGAGCGATATGTCATTTAAGTAAAGTTACTGT260.10588043655318456No Hit
TTAGTGGGGTCTGACAAGCTGATGGGAGAGAAGAATAGAGCAGATTGCAGTA250.10180811207036976No Hit
TTGTATGGGTGCGACTTGATACTTGGAAATGTGTGTGACTTTTACAAAGAGC250.10180811207036976No Hit
GGTAACGGGATATTGGGTTATTCACCATAAAGGATCCAATAGCAATTCTAAA250.10180811207036976No Hit
ACGCCTGGGTGGAAATTGACGAAGTATAATTATTCTAAGATGGAATAATTAA250.10180811207036976No Hit
GGCTTAGGGAGGGTTAATAATTGTTTCTCCTTTTTAATTTGCTTTATTTCCT250.10180811207036976No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)