FastQCFastQC Report
Sat 31 Dec 2022
SRR5844265.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844265.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23192
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGTATGCCGTCT4011.7290444981027941No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGTATGC3641.5695067264573992No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTGTCTCTTATACA1150.4958606416005519No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTGTCTCTT910.3923766816143498No Hit
ATGCGAGGGACAAGGAATATAGGCAGATGTACAGAACTGAAATTCGAATGGC840.3621938599517075No Hit
AGCTAGGGAGACCTAGTTCTATTATCATTTGTTAGTTCACTGTGCTCAAGTC840.3621938599517075No Hit
AGGCACGGGATGGCCGCGGGCCGCCTCTTTCTAAGTCGGCTTCGAGCACCCT780.336322869955157No Hit
GGAACTGGGGATGGACCAGGAGCCAGTGGGCGGTGTGGAACGAGGAGAAGCC770.3320110382890652No Hit
GAAACGGGGACAGTCCCTATTTCCTTTGCTGTGTTCCCCAATGAAGATGGCT770.3320110382890652No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGG750.3233873749568817No Hit
TGTGCTGGGAATCTACTGTTTGTGAATGTCTGTATTATCAGGAAACATGTCT750.3233873749568817No Hit
CAGTATGGGATTTAAGCACTTAGGCAGAGTAGACACAATCAAAGGTACAAAG740.3190755432907899No Hit
ATACAGGGAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAACAA730.3147637116246982No Hit
TTAGCTGGGATACATGGTACACAGAGAGGGGTGTCACTTCAGAAAATCTTCC720.3104518799586064No Hit
AGAGCCGGGACCCCGTTTCTACCAAAAATACAAAAATTAGCCGGGCGTGGTG700.3018282166264229No Hit
GCATATGGGAATTGAAATGAGATGAGTTGAAATGATGAGATGTAATGGTGAA690.29751638496033117No Hit
CGTTACGGGGGGAAAGACGGTGAATGGAAAGGGGATGGCTGGTGGTCTTGGG670.28889272162814766No Hit
TGACAAGGGACCTGACATTATCTCAGTTTTCTCCTATATTTTAGGAATTTTA670.28889272162814766No Hit
ATCGCTGGGATTGTAAATTTTGAGATTTTATTTAGAAATGAATTGTCTAATT660.2845808899620559No Hit
TCATCCGGGAGGGATGGAGATGGGTCAGGCCTGGGCTTTGCCTGGGGAAGCC650.2802690582959641No Hit
GTCGCCTGGGAGAGGCCATGGGCACGGCAGGCGGGCCTGGCCACCCTGTACA650.2802690582959641No Hit
TGCTGGGGGCAAATTTAGTTTTCACAGCATGGTTTTGTTGTTTTCCCCTTGT640.2759572266298724No Hit
GAAGCCGGGGGGAGAGGGCTACCATGGGGGTGAGGGGAGGCCGCCCGCCGCG630.2716453949637806No Hit
TCTTCCATAGGGCTCCTGCTTCAGCCTGTCGAGTAGCTGGGATTATAGGCAT620.26733356329768887No Hit
TTTGCAGGGAGGCTTTGTTGGTCATACAGTCTCTGTCACAACTAATTAACTC620.26733356329768887No Hit
ATTTTCGGGAGCACTCAAACCTGGAGCTGAGGAATCTAATTCAGACAGAGAC620.26733356329768887No Hit
TGTGGCGGGATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTT620.26733356329768887No Hit
ACTGCTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC610.2630217316315971No Hit
TTTTGCGGGCCCTCTGACATCCGGCCTGCTTCTTCTCACATGACAAAAACTA600.25870989996550536No Hit
AACACAGGGCAATTGTCATTAGCAACATGGCTGAGCCTTTCAGTGCCACAGA570.2457744049672301No Hit
AACCATGGGACCTCTGTAAACTATTCCTAAAATGTGTATGATATATTTGAAA560.2414625733011383No Hit
GCCCGGGGGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAA560.2414625733011383No Hit
AAGTCTGGGCACAGCATGCATAAATTGCACATTTTGTTTTACTTTCTTTATA540.2328389099689548No Hit
TTTGCCGGGATTTCATCTTGGGAAGTGCCCCAATCCTCCGGTGCAGGAGAAT540.2328389099689548No Hit
GTCTCTGGGGGTAACAACTGAAAGGGAAAAGTGCTTCAGCCATCTTTTGAAA530.22852707830286306No Hit
TGTGTCTGGGCTGTGGTGACAGTTTGTGACATTCTGTCTTCATGAGGTCTCA530.22852707830286306No Hit
TAAAATGGGATTAATACTAGGTTTTACATTTACCTATGTAGTTACCTTATCA520.2242152466367713No Hit
AATTCAGGGAAATTAAAAATATGTGGGGGAGAGATCAGTGAAAAGTGGTTTT520.2242152466367713No Hit
TTTTAGGGGATACTAAAATGCTGCGTTAATTTTAGGCCATTGAAAGCTGTAC520.2242152466367713No Hit
TATTGGGGGAGCTAGGAATACCACTGATTTAGAAACAAAACTGTTTGTCTCT510.21990341497067956No Hit
ATACTTGGGAAGACAGCCGTCTGTGACATCCCACCTCGTGGCCTCAAGATGG510.21990341497067956No Hit
TCAACTGGGATGTTAAACACCAGTAGAGAAACAACTATGGTTGTGATTAAAT500.2155915833045878No Hit
GGCTTAGGGAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTC490.21127975163849605No Hit
ATTTGGGATTGGATGCTAAAGCTGATATGAGCCTCCAAGTGCTCAGAGGCTG490.21127975163849605No Hit
TAAGCAGGGAGCTCATCTGTCAATTCTGTCAACTACTCAGGATCCTCTCAAG490.21127975163849605No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGA480.2069679199724043No Hit
GTTAGGGGGGGCAGATCACTTGAGCTTGGGGGTTTGAGACCAGCCTGGGCAA480.2069679199724043No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGT470.20265608830631254No Hit
TATGTTGGGACCCTTAAGACTGACATTTTCTCGTTAGGGAAAGAGACTCGAT470.20265608830631254No Hit
CAATAGGGGATGGGAATAAAGTTATTTCAATAGTTGACTCTTTTGAAAAGTT470.20265608830631254No Hit
TCTTGCGGGCATACAGACAAGGGGAAAAAGACGGCCTGGTTTTAAACCCCAG470.20265608830631254No Hit
GCTTATGGGCTGATGCTGAACTGACACCAGGCGAATGTCAGGGCTCCCAAAC460.19834425664022073No Hit
TTACATGGGAGGATGAATAATAGCAGTTCTACCGTACAACCCTAACATAACC450.194032424974129No Hit
GTTAAAGGATTTAAGGTAAATTATAAAACCTAAAATTAGTATCAGTATTTTA450.194032424974129No Hit
AGAGTTGGGATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC440.18972059330803726No Hit
GGCTTCGGGGAAGAAAACTTCCAGTTAAAAAAAATTGGATAATGTTTTTGAT440.18972059330803726No Hit
AGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACACCACCTTC440.18972059330803726No Hit
ATAATAGGGATCTCATCCTTTATTTTTAATCACTTTTTTTCTATGATGGATA430.1854087616419455No Hit
ATCTTGGGGGAGGCGAGAGCTGCGAAAGGCGAGAGCTGCGAAGGGCCAGGTG430.1854087616419455No Hit
AAGATAGGGAAGCCTGTGGGAGTAGAAAAATCTCATTCCCAGAGTCAGAGGA420.18109692997585375No Hit
ACTTACGGGAGGATCAAAATTAATTTCAAACCTTCCAACCAACCCTATAAGC420.18109692997585375No Hit
ATTTGCGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAA410.176785098309762No Hit
ATTCGGGGAGAACATTTGTTAAAACACAGATCAGAAACAGAGTTTCTGACTC410.176785098309762No Hit
TGACAAGGGGGGGGCCCAGGTTGGATGGGGAAAGGCTAATGGGGTCAGACCC410.176785098309762No Hit
ACTTCTGGGACTGCAACTAACTGTTGCCCCCCAACCCCGAACCCCCAGTTGA410.176785098309762No Hit
GTTAGTGGGAATCCCAGTACTCTGGGAGGCCGAGGTGGGAGGATCACCTGAG410.176785098309762No Hit
GTCTTAGGGCAAAACTGTAAGCAGGATTGTCTGTAAGAATCACATTTCTATC400.17247326664367024No Hit
GTAGGCGGGTAAAAAATGTATTTAAAAGAAAATTGAGAGAAAGGACTACAGA400.17247326664367024No Hit
AAAAGTGGGCACTAGACTTGGCTTATACAAAATGCTAAAGGGAGTCCTTCAA400.17247326664367024No Hit
ACCATAGGGAGTCAGTGTCCGGCCAACTCTGCAGCTCGGTCCAGCCCTGCCC400.17247326664367024No Hit
TGCTATGGGAATAATGGAGTAGCTGTTGCCCTTCTCCGGTTGTGTGTACAGT390.1681614349775785No Hit
AGTTATGGGATCTTGCCTTTTTTCAGGCTAGCTGCAATAATTTTTTTCTTCT390.1681614349775785No Hit
CGCGCAGGGAAACCTTTGAGTCTAATAAAGCTTTCTGCAACTGAGGAACTTC390.1681614349775785No Hit
AGCGATGGGATTGCCGAGACTGGTCTCAAACTCCTGGAGTCAAGTGATCCTC390.1681614349775785No Hit
TCAGCCGGGATCTTAGGTTGGAGATGACAGAAATATGATTGATTTGAAGTGG390.1681614349775785No Hit
GGTGCAGGGGAGCGCCTGTTAAAGCAACAGTGTAAGATTCTTTATATTGCAT390.1681614349775785No Hit
AGTTCTGGGAGTGTGGGTCCACAGGGCATAGGAACTTATGTTTTTGTTCCTA380.1638496033114867No Hit
GTCAACGGGATTGCTTGGATTTCTAGTGAGAGCTGTTGAATTTGGTGATGTC380.1638496033114867No Hit
TTCGCAGGGCACTAGCAGAGACCAACCGAACCCCCTTCGACCTTGCCGAAGG380.1638496033114867No Hit
CCCGCGGTACTACAAGAACATCGGTCTGGGCTTCAAGACACCCAAGGAGGCT380.1638496033114867No Hit
CTTTATGGGATGGATGGTAGGAGGCACCCGGGTCTAAGGTGAGACTCTTTGT380.1638496033114867No Hit
AGATCAGGGAAAATTGTCAACTTGAAACTAAAACATGCCAAGGTTTTGATAT370.15953777164539495No Hit
TTATGTGGGCATAGCTTATTTGTCAAAGCAGCACACCTGTGAGGATTCCTGT370.15953777164539495No Hit
AAGCGTGGGCTGATATAAATTTGGTGGCTGATGATTCAATTTAATGTAACAA370.15953777164539495No Hit
AATAGTGGGGGGGTGGGGAACATGTCTGAGTCGGAGCTCGGCAGGAAGTGGG370.15953777164539495No Hit
ATAGCAGGAGTAATGGTGCAGCATTGCCTGATGGAAATATAGCTGAGAGCTT370.15953777164539495No Hit
AGACAAGGGATGTGGCCTCTCCTTGAAAATATTGCATGTGCTTATGAAAAAA370.15953777164539495No Hit
CTGATCGGGGTGCATAACCAGTCATGAGATAAAACAACACAATGCATGTTGC360.1552259399793032No Hit
AGCGCGGGAAATTTGAATATTTTAATCCGTTTATATACTATTTAATATTGCT360.1552259399793032No Hit
TTTGCGGAGACAATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACAT350.15091410831321145No Hit
GGATGTGGGACCTATGGAAAAAATCAATAATCTTTGGGATCTAATTTCTACA350.15091410831321145No Hit
TGGTACCGGGATTGTATATTTTATGTTTGTAAATTGTTTAGAAAGATAATTG350.15091410831321145No Hit
CAGCCAGGGAGAATAACTTATTTCAGAAATGAGGAAACTGAGACTTATGATC350.15091410831321145No Hit
TCACACGGGGAGGAAAACTAAATTCTCAAATAATTCATAATGGGCGAAGTGC350.15091410831321145No Hit
TTGCCAGGGATACGGGGGAGGGTCAGGGAAGAACGAACCTTGACGTTGCAGT340.1466022766471197No Hit
GGTCTTGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCT340.1466022766471197No Hit
TCTAGCGGGAGATCATGGATATAAAGAGATACCTGATTTTTATTAAAAAGAT340.1466022766471197No Hit
CTTAATCGGGGTCTTAGCATACTCCTCAATTACCCACATAGGATGAATAATA330.14229044498102794No Hit
CTTCACGGGAAGTGACACTACAGAAAAACACAAAAAGGTGATGGGTTGTGTT330.14229044498102794No Hit
GATACTGGGAGATATATAAATAATCACACACACACTTGCTGTCAATGTTTTG320.1379786133149362No Hit
GCAATGGGACTCTAACAAGCTCTGTCCACGGGTCTCCCCATTCCCACCAGGC320.1379786133149362No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGAACTCGTATGC320.1379786133149362No Hit
TGCCTCGGGAAGCTATTGCATGATTAGCCCCCTATCTGGCAAGGAAACCCCA310.13366678164884443No Hit
TTCTTAGGGAGCCCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCC310.13366678164884443No Hit
GCTACTGGGACTGCATGACACATGGCCTGGTTACCTGTCATGGAATGCTGCA310.13366678164884443No Hit
AGAAATGGGAAGAAGTGACTGTTGTTAAGATAGACATTGAAGGACAGCAAAA310.13366678164884443No Hit
TTATTGGGACTCTGGCAAGACTGAAGATGAGGTACTTTTGTTTGTTTGGGTG310.13366678164884443No Hit
CAATCAGGGAGAGTAGGGAGCTGATAACAGTCCCAAGCCCTCCTCTTTCTCT310.13366678164884443No Hit
TAGAGGGGCCTAGGAAGTTACTATGGGAAAAGTGGAAATAGTTGAGGCCCAG300.12935494998275268No Hit
AGTACAGGGAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT300.12935494998275268No Hit
AGTGAGGGGAAATTGATAACTTGCCTCTTTATCTTTGGCAACTCTCCCATGT300.12935494998275268No Hit
CGAAGGGACCTTACAAATTTCAAAGAATACAAACCATATAAAGTGTGCTCTC300.12935494998275268No Hit
TCTATCGGGAGGTGGGGATAAAATCTCATACATGGATTTTTTCGTGTCCGCT290.12504311831666093No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCCATGGATCTCGT290.12504311831666093No Hit
ACCATGGGCACCTTGTCCTCTCAAAATGCTTACAGGCATGAACCACTGTGAC290.12504311831666093No Hit
TAAAGAGGGCACAGGTGCACCCTTCCCACACCCTTTACATTCCCCAGAGCCA280.12073128665056915No Hit
CGTATGGGGGAATATTTAGGATTTGGTGAAGTTCACTGTATTGCTATATTTT280.12073128665056915No Hit
GTCAATGGGTGCCAAATTTGTACTTTGGAATCCTCCTCTGCATGTTCATAAG280.12073128665056915No Hit
CTTGCTAGGGACATCAATGTAGAACTCAGCCTTCTTGGAAAGAAAAAAAAGA280.12073128665056915No Hit
GAACGTGGGAACTCACAGATGTTTCAGTTCATATTAGAATAAATTGCCCAAT280.12073128665056915No Hit
ATGCACGGGAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACC280.12073128665056915No Hit
CGGTTAGGGCACTTAATTTTTTACACAAACGCCCAACTATTTTCCTGAGAGT270.1164194549844774No Hit
TCTTGGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG270.1164194549844774No Hit
ACCCAAGGGATGACTAATCATCAGGGTTTTAGTCTAATTAAAACCACAGTGA270.1164194549844774No Hit
CCTTTAGGGAAACATCTTCAGGTTTTAGAAAACTAGACCCATGTTGAGTCCA260.11210762331838565No Hit
TCCTAGGGGATTATACCAGGATAGGGAAATTTTTCAAGGATGATTTAGGAAA260.11210762331838565No Hit
TAAGGTGGGATTATTCATTTTGATGCACAAATTGTTTCAAATTTAACAAGAG260.11210762331838565No Hit
CACGGAGGGAATGGGTGGACTCACACTTGCTCAGTTCAGGCAGCTCTGCTGG260.11210762331838565No Hit
GTGATGGGCGATCTCAAAATGGGAGAGGTAACAGATGCTCAGGACAGCTGCA260.11210762331838565No Hit
TTGCCAGGGAGTGAAATGTGGAAGTCAACCAGACCAATGATGGAATTAAATG260.11210762331838565No Hit
TCTAGAGGGATAAAATGAAAAGCTATACTACTTTTTATAAAAGGGCAAACTG260.11210762331838565No Hit
TAGTGCGGGAGGGTGGCTCATTTGGACGAAGTATTGTTAAGGTGGTTATTAG250.1077957916522939No Hit
GTCAACGGGGAGTTGCACAAATCTAACTGGTATTTTGTTTTTGTTTTTAGGA250.1077957916522939No Hit
GCTGTAGGGAGATTACTAAGTCATTTGTATGAATATGTGTGGCAGTGAAGAG250.1077957916522939No Hit
GGCACAGGGAGTGGGAGAACACAGAGACTAACTGGCATAAGGAAAAGATGGA250.1077957916522939No Hit
GGCTGCTCGGGACAGATGGATGTAGTGAATGCTTTCCAGAGTGGAAGTTCCA250.1077957916522939No Hit
GTAGCCGGGATTATAATTTAGTTCATACTGAAAAAGAAATTAACAGAACATT240.10348395998620215No Hit
CAAGGAGGGATTCTCACCCTATTAAATGGAAATTTAGAGCACCCTTAAATTC240.10348395998620215No Hit
CAAACCGGGCTCTACGGGGGGACAAGAAATCTGAGAGATCTAGTGTAGATAA240.10348395998620215No Hit
GACCCGGGAGATTTACAAACATGTCATAGTTGACTTAAGCGCATGAGACACC240.10348395998620215No Hit
TTCAAAGGGAGTTCACTGCCATGTTCCGCCGGAAGGCCTTCCTCCACTGGTA240.10348395998620215No Hit
TATACGGGGTGACTATAAAAGATTGGGGGGAAATAATTAAACTACTCTAAGC240.10348395998620215No Hit
CTGTACGGGATCCCATCACCCCAAACATACCCTAGTCCTTCAGCCTGGGGCT240.10348395998620215No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)